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Protein

UV excision repair protein RAD23

Gene

RAD23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role both in proteasomal degradation of misfolded proteins and DNA repair. Central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in DNA excision repair. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes.

Miscellaneous

Present with 10900 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: SGD
  • polyubiquitin modification-dependent protein binding Source: GO_Central
  • proteasome binding Source: SGD
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30158-MONOMER
ReactomeiR-SCE-5696394 DNA Damage Recognition in GG-NER
R-SCE-5696395 Formation of Incision Complex in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23
Gene namesi
Name:RAD23
Ordered Locus Names:YEL037C
ORF Names:SYGP-ORF29
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

SGDiS000000763 RAD23

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149021 – 398UV excision repair protein RAD23Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei94PhosphothreonineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei139PhosphothreonineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32628
PaxDbiP32628
PRIDEiP32628

PTM databases

iPTMnetiP32628

Interactioni

Subunit structurei

Interacts directly with PNG1.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36692, 225 interactors
ComplexPortaliCPX-1320 Peptide:N-glycanase-Rad23 complex
CPX-1323 CDC48-RAD23-UFD2 complex
CPX-1640 Nucleotide excision repair factor 2 complex
DIPiDIP-1548N
IntActiP32628, 39 interactors
MINTiP32628
STRINGi4932.YEL037C

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32628
SMRiP32628
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32628

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 77Ubiquitin-likePROSITE-ProRule annotationAdd BLAST77
Domaini146 – 186UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini355 – 395UBA 2PROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000012078
HOGENOMiHOG000172162
InParanoidiP32628
KOiK10839
OMAiDFLVLMV
OrthoDBiEOG092C4GFV

Family and domain databases

Gene3Di1.10.10.540, 1 hit
InterProiView protein in InterPro
IPR004806 Rad23
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR015360 XPC-bd
IPR036353 XPC-bd_sf
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00240 ubiquitin, 1 hit
PF09280 XPC-binding, 1 hit
PRINTSiPR01839 RAD23PROTEIN
SMARTiView protein in SMART
SM00727 STI1, 1 hit
SM00165 UBA, 2 hits
SM00213 UBQ, 1 hit
SUPFAMiSSF101238 SSF101238, 1 hit
SSF46934 SSF46934, 2 hits
SSF54236 SSF54236, 1 hit
TIGRFAMsiTIGR00601 rad23, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32628-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV
60 70 80 90 100
LQDSKTVSEC GLKDGDQVVF MVSQKKSTKT KVTEPPIAPE SATTPGRENS
110 120 130 140 150
TEASPSTDAS AAPAATAPEG SQPQEEQTAT TERTESASTP GFVVGTERNE
160 170 180 190 200
TIERIMEMGY QREEVERALR AAFNNPDRAV EYLLMGIPEN LRQPEPQQQT
210 220 230 240 250
AAAAEQPSTA ATTAEQPAED DLFAQAAQGG NASSGALGTT GGATDAAQGG
260 270 280 290 300
PPGSIGLTVE DLLSLRQVVS GNPEALAPLL ENISARYPQL REHIMANPEV
310 320 330 340 350
FVSMLLEAVG DNMQDVMEGA DDMVEGEDIE VTGEAAAAGL GQGEGEGSFQ
360 370 380 390
VDYTPEDDQA ISRLCELGFE RDLVIQVYFA CDKNEEAAAN ILFSDHAD
Length:398
Mass (Da):42,367
Last modified:October 1, 1993 - v1
Checksum:iB3F0436DAB60B833
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277A → R in AAA34935 (PubMed:8411151).Curated1
Sequence conflicti277A → R in AAA34938 (PubMed:8411151).Curated1
Sequence conflicti277A → R in AAD13972 (PubMed:8411151).Curated1
Sequence conflicti277A → R in AAB28441 (PubMed:8411151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25428 Unassigned DNA Translation: AAA16070.1
L22172 Genomic DNA Translation: AAA34935.1
L22173 Genomic DNA Translation: AAA34938.1
S65964 Genomic DNA Translation: AAD13972.1
S66117 mRNA Translation: AAB28441.1
U18779 Genomic DNA Translation: AAB65005.1
AY693018 Genomic DNA Translation: AAT93037.1
BK006939 Genomic DNA Translation: DAA07616.1
PIRiS50507
RefSeqiNP_010877.3, NM_001178852.3

Genome annotation databases

EnsemblFungiiYEL037C; YEL037C; YEL037C
GeneIDi856674
KEGGisce:YEL037C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25428 Unassigned DNA Translation: AAA16070.1
L22172 Genomic DNA Translation: AAA34935.1
L22173 Genomic DNA Translation: AAA34938.1
S65964 Genomic DNA Translation: AAD13972.1
S66117 mRNA Translation: AAB28441.1
U18779 Genomic DNA Translation: AAB65005.1
AY693018 Genomic DNA Translation: AAT93037.1
BK006939 Genomic DNA Translation: DAA07616.1
PIRiS50507
RefSeqiNP_010877.3, NM_001178852.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X3WX-ray3.00B238-309[»]
1X3ZX-ray2.80B238-309[»]
2NBUNMR-A1-78[»]
2NBWNMR-B1-78[»]
2QSFX-ray2.35X230-398[»]
2QSGX-ray3.10X230-398[»]
2QSHX-ray2.81X230-398[»]
3ESWX-ray3.40B254-308[»]
3M62X-ray2.40B1-84[»]
4YIRX-ray3.05X230-398[»]
ProteinModelPortaliP32628
SMRiP32628
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36692, 225 interactors
ComplexPortaliCPX-1320 Peptide:N-glycanase-Rad23 complex
CPX-1323 CDC48-RAD23-UFD2 complex
CPX-1640 Nucleotide excision repair factor 2 complex
DIPiDIP-1548N
IntActiP32628, 39 interactors
MINTiP32628
STRINGi4932.YEL037C

PTM databases

iPTMnetiP32628

Proteomic databases

MaxQBiP32628
PaxDbiP32628
PRIDEiP32628

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL037C; YEL037C; YEL037C
GeneIDi856674
KEGGisce:YEL037C

Organism-specific databases

SGDiS000000763 RAD23

Phylogenomic databases

GeneTreeiENSGT00390000012078
HOGENOMiHOG000172162
InParanoidiP32628
KOiK10839
OMAiDFLVLMV
OrthoDBiEOG092C4GFV

Enzyme and pathway databases

BioCyciYEAST:G3O-30158-MONOMER
ReactomeiR-SCE-5696394 DNA Damage Recognition in GG-NER
R-SCE-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

EvolutionaryTraceiP32628
PROiPR:P32628

Family and domain databases

Gene3Di1.10.10.540, 1 hit
InterProiView protein in InterPro
IPR004806 Rad23
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR015360 XPC-bd
IPR036353 XPC-bd_sf
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00240 ubiquitin, 1 hit
PF09280 XPC-binding, 1 hit
PRINTSiPR01839 RAD23PROTEIN
SMARTiView protein in SMART
SM00727 STI1, 1 hit
SM00165 UBA, 2 hits
SM00213 UBQ, 1 hit
SUPFAMiSSF101238 SSF101238, 1 hit
SSF46934 SSF46934, 2 hits
SSF54236 SSF54236, 1 hit
TIGRFAMsiTIGR00601 rad23, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS50053 UBIQUITIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD23_YEAST
AccessioniPrimary (citable) accession number: P32628
Secondary accession number(s): D3DLL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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Main funding by: National Institutes of Health

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