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Protein

Guanosine-diphosphatase

Gene

GDA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.1 Publication

Miscellaneous

Present with 8680 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GDP + H2O = GMP + phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei216Proton acceptorBy similarity1

GO - Molecular functioni

  • guanosine-diphosphatase activity Source: SGD
  • uridine-diphosphatase activity Source: SGD

GO - Biological processi

  • protein glycosylation Source: SGD

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciYEAST:YEL042W-MONOMER
ReactomeiR-SCE-8850843 Phosphate bond hydrolysis by NTPDase proteins
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-diphosphatase (EC:3.6.1.42)
Short name:
GDPase
Gene namesi
Name:GDA1
Ordered Locus Names:YEL042W
ORF Names:SYGP-ORF16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL042W
SGDiS000000768 GDA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST15
Topological domaini25 – 518LumenalSequence analysisAdd BLAST494

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002099191 – 518Guanosine-diphosphataseAdd BLAST518

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32621
PaxDbiP32621
PRIDEiP32621

PTM databases

iPTMnetiP32621

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA2P105922EBI-7511,EBI-8603

Protein-protein interaction databases

BioGridi36687, 187 interactors
DIPiDIP-5284N
IntActiP32621, 17 interactors
MINTiP32621
STRINGi4932.YEL042W

Structurei

3D structure databases

ProteinModelPortaliP32621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046675
HOGENOMiHOG000206583
InParanoidiP32621
KOiK01526
OMAiTPRGYLT
OrthoDBiEOG092C1F34

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit

Sequencei

Sequence statusi: Complete.

P32621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPIFRNYRF AIGAFAVIML ILLIKTSSIG PPSIARTVTP NASIPKTPED
60 70 80 90 100
ISILPVNDEP GYLQDSKTEQ NYPELADAVK SQTSQTCSEE HKYVIMIDAG
110 120 130 140 150
STGSRVHIYK FDVCTSPPTL LDEKFDMLEP GLSSFDTDSV GAANSLDPLL
160 170 180 190 200
KVAMNYVPIK ARSCTPVAVK ATAGLRLLGD AKSSKILSAV RDHLEKDYPF
210 220 230 240 250
PVVEGDGVSI MGGDEEGVFA WITTNYLLGN IGANGPKLPT AAVFDLGGGS
260 270 280 290 300
TQIVFEPTFP INEKMVDGEH KFDLKFGDEN YTLYQFSHLG YGLKEGRNKV
310 320 330 340 350
NSVLVENALK DGKILKGDNT KTHQLSSPCL PPKVNATNEK VTLESKETYT
360 370 380 390 400
IDFIGPDEPS GAQCRFLTDE ILNKDAQCQS PPCSFNGVHQ PSLVRTFKES
410 420 430 440 450
NDIYIFSYFY DRTRPLGMPL SFTLNELNDL ARIVCKGEET WNSVFSGIAG
460 470 480 490 500
SLDELESDSH FCLDLSFQVS LLHTGYDIPL QRELRTGKKI ANKEIGWCLG
510
ASLPLLKADN WKCKIQSA
Length:518
Mass (Da):56,822
Last modified:October 1, 1993 - v1
Checksum:i9A61182D5ED22ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19560 Genomic DNA Translation: AAA34656.1
U18779 Genomic DNA Translation: AAB65000.1
AY723798 Genomic DNA Translation: AAU09715.1
BK006939 Genomic DNA Translation: DAA07611.1
PIRiA40732
RefSeqiNP_010872.1, NM_001178857.1

Genome annotation databases

EnsemblFungiiYEL042W; YEL042W; YEL042W
GeneIDi856669
KEGGisce:YEL042W

Similar proteinsi

Entry informationi

Entry nameiGDA1_YEAST
AccessioniPrimary (citable) accession number: P32621
Secondary accession number(s): D3DLK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 18, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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