Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase PP1-2

Gene

GLC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in control of glycogen metabolism, meiosis, translation, chromosome segregation, cell polarity and G2/M cell cycle progression. PP1 may act in opposition to the IPL1 protein kinase in regulating chromosome segregation by dephosphorylating H3S10ph. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex.
Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation.

Miscellaneous

Present with 14600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Manganese 1By similarity1
Metal bindingi65Manganese 1By similarity1
Metal bindingi91Manganese 1By similarity1
Metal bindingi91Manganese 2By similarity1
Metal bindingi123Manganese 2By similarity1
Active sitei124Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCarbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism, Mitosis, mRNA processing
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YER133W-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-2 (EC:3.1.3.16)
Gene namesi
Name:GLC7
Synonyms:CID1, DIS2
Ordered Locus Names:YER133W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

SGDiS000000935 GLC7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73R → C in GLC7-1; decreased glycogen accumulation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588201 – 312Serine/threonine-protein phosphatase PP1-2Add BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki47Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32598
PaxDbiP32598
PRIDEiP32598

PTM databases

iPTMnetiP32598

Interactioni

Subunit structurei

Probably complexed with regulatory or targeting subunits. Interacts with BUD14. Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2. Interacts with YPI1.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi36877, 680 interactors
ComplexPortaliCPX-1053 Cleavage and polyadenylation specificity factor complex
CPX-1230 BNI4-GLC7 phosphatase complex
CPX-1231 GAC1-GLC7 phosphatase complex
CPX-1249 SDS22-GLC7 phosphatase complex
CPX-1260 SDS22-GLC7-YPI1 phosphatase complex
CPX-1266 REG1-GLC7 phosphatase complex
CPX-1267 REG2-GLC7 phosphatase complex
CPX-1705 BUD14-GLC7 phosphatase complex
DIPiDIP-1336N
ELMiP32598
IntActiP32598, 182 interactors
MINTiP32598
STRINGi4932.YER133W

Structurei

3D structure databases

ProteinModelPortaliP32598
SMRiP32598
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000062911
HOGENOMiHOG000172697
InParanoidiP32598
KOiK06269
OMAiEEHEIRY
OrthoDBiEOG092C37EX

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR037981 PPP1CC
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PANTHERiPTHR11668:SF204 PTHR11668:SF204, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P32598-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSQPVDVDN IIDRLLEVRG SKPGQQVDLE ENEIRYLCSK ARSIFIKQPI
60 70 80 90 100
LLELEAPIKI CGDIHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFIL RGNHECASIN RIYGFYDECK RRYNIKLWKT
160 170 180 190 200
FTDCFNCLPI AAIIDEKIFC MHGGLSPDLN SMEQIRRVMR PTDIPDVGLL
210 220 230 240 250
CDLLWSDPDK DIVGWSENDR GVSFTFGPDV VNRFLQKQDM ELICRAHQVV
260 270 280 290 300
EDGYEFFSKR QLVTLFSAPN YCGEFDNAGA MMSVDESLLC SFQILKPAQK
310
SLPRQAGGRK KK
Length:312
Mass (Da):35,907
Last modified:October 1, 1993 - v1
Checksum:i71B20E3A4ED93235
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137D → H in AAA34570 (PubMed:2544298).Curated1
Sequence conflicti273G → V in AAA34570 (PubMed:2544298).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77175 Genomic DNA Translation: AAB59322.1
M27070 Genomic DNA Translation: AAA34570.1
U18916 Genomic DNA Translation: AAC03231.1
BK006939 Genomic DNA Translation: DAA07793.1
PIRiS32595
RefSeqiNP_011059.3, NM_001179023.3

Genome annotation databases

EnsemblFungiiYER133W; YER133W; YER133W
GeneIDi856870
KEGGisce:YER133W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77175 Genomic DNA Translation: AAB59322.1
M27070 Genomic DNA Translation: AAA34570.1
U18916 Genomic DNA Translation: AAC03231.1
BK006939 Genomic DNA Translation: DAA07793.1
PIRiS32595
RefSeqiNP_011059.3, NM_001179023.3

3D structure databases

ProteinModelPortaliP32598
SMRiP32598
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36877, 680 interactors
ComplexPortaliCPX-1053 Cleavage and polyadenylation specificity factor complex
CPX-1230 BNI4-GLC7 phosphatase complex
CPX-1231 GAC1-GLC7 phosphatase complex
CPX-1249 SDS22-GLC7 phosphatase complex
CPX-1260 SDS22-GLC7-YPI1 phosphatase complex
CPX-1266 REG1-GLC7 phosphatase complex
CPX-1267 REG2-GLC7 phosphatase complex
CPX-1705 BUD14-GLC7 phosphatase complex
DIPiDIP-1336N
ELMiP32598
IntActiP32598, 182 interactors
MINTiP32598
STRINGi4932.YER133W

PTM databases

iPTMnetiP32598

Proteomic databases

MaxQBiP32598
PaxDbiP32598
PRIDEiP32598

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER133W; YER133W; YER133W
GeneIDi856870
KEGGisce:YER133W

Organism-specific databases

SGDiS000000935 GLC7

Phylogenomic databases

GeneTreeiENSGT00530000062911
HOGENOMiHOG000172697
InParanoidiP32598
KOiK06269
OMAiEEHEIRY
OrthoDBiEOG092C37EX

Enzyme and pathway databases

BioCyciYEAST:YER133W-MONOMER

Miscellaneous databases

PROiPR:P32598

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR037981 PPP1CC
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PANTHERiPTHR11668:SF204 PTHR11668:SF204, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPP12_YEAST
AccessioniPrimary (citable) accession number: P32598
Secondary accession number(s): D3DM39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again