STE14

Functionⁱ

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functionⁱ

protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity
Source: SGD
Inferred from direct assayⁱ
- 2050108

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

C-terminal protein methylation
Source: SGD
Inferred from mutant phenotypeⁱ
- 2173693
peptide pheromone maturation
Source: SGD
Inferred from mutant phenotypeⁱ
- 2050108
response to pheromone Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Pheromone response
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29953-MONOMER
Reactomeⁱ	R-SCE-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100) Alternative name(s): Isoprenylcysteine carboxylmethyltransferase Prenylated protein carboxyl methyltransferase Short name: PPMT Prenylcysteine carboxyl methyltransferase Short name: pcCMT
Gene namesⁱ	Name:STE14 Ordered Locus Names:YDR410C ORF Names:D9461.1
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000002818 STE14

SGDⁱ

S000002818 STE14

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 23	ExtracellularSequence analysisAdd BLAST	23
Transmembraneⁱ	24 – 44	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	45 – 47	CytoplasmicSequence analysis	3
Transmembraneⁱ	48 – 68	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	69 – 88	ExtracellularSequence analysisAdd BLAST	20
Transmembraneⁱ	89 – 109	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	110	CytoplasmicSequence analysis	1
Transmembraneⁱ	111 – 131	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	132 – 182	ExtracellularSequence analysisAdd BLAST	51
Transmembraneⁱ	183 – 203	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	204 – 239	CytoplasmicSequence analysisAdd BLAST	36

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL4385

ChEMBLⁱ

CHEMBL4385

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000209899	1 – 239	Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST		239

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P32584
PaxDbⁱ	P32584
PRIDEⁱ	P32584

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	32470, 19 interactors
Database of interacting proteins More...DIPⁱ	DIP-2678N
IntActⁱ	P32584, 3 interactors
Molecular INTeraction database More...MINTⁱ	P32584
STRINGⁱ	4932.YDR410C

Chemistry databases

BindingDBⁱ

P32584

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P32584
SMRⁱ	P32584
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017394
HOGENOMⁱ	HOG000213961
InParanoidⁱ	P32584
KEGG Orthology (KO) More...KOⁱ	K00587
OMAⁱ	LPFIRGY
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C5DVA

Family and domain databases

InterProⁱ	View protein in InterPro IPR007269 ICMT_MeTrfase IPR025770 PPMT_MeTrfase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04140 ICMT, 1 hit
PROSITEⁱ	View protein in PROSITE PS51564 SAM_ICMT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P32584-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN 
        60         70         80         90        100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL 
       110        120        130        140        150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI 
       160        170        180        190        200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF 
       210        220        230 
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI

Length:239

Mass (Da):27,888

Last modified:October 1, 1993 - v1

Checksum:ⁱ189829AFD0BD6BD5

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L07952 Unassigned DNA Translation: AAA16520.1 L15442 Unassigned DNA Translation: AAA16840.1 U33007 Genomic DNA Translation: AAB64880.1 BK006938 Genomic DNA Translation: DAA12252.1
PIRⁱ	S37604
NCBI Reference Sequences More...RefSeqⁱ	NP_010698.1, NM_001180718.1

Genome annotation databases

EnsemblFungiⁱ	YDR410C; YDR410C; YDR410C
GeneIDⁱ	852019
KEGGⁱ	sce:YDR410C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P32584	Protein-S-isoprenylcysteine O-methyltransferase		YEASC		239	UniRef100_P32584

Protein	Similar proteins		Species	Score	Length	Source
P32584	Protein-S-isoprenylcysteine O-methyltransferase		YEASC		239	UniRef90_P32584
Protein-S-isoprenylcysteine O-methyltransferase		YEASO		255
Protein-S-isoprenylcysteine O-methyltransferase		YEASL		239
Protein-S-isoprenylcysteine O-methyltransferase		YEASV		239
Protein-S-isoprenylcysteine O-methyltransferase		SACCK		151
+10

Protein	Similar proteins		Species	Score	Length	Source
P32584	Protein-S-isoprenylcysteine O-methyltransferase		YEASC		239	UniRef50_P32584
Protein-S-isoprenylcysteine O-methyltransferase		YEASO		255
Protein-S-isoprenylcysteine O-methyltransferase		YEASL		239
Protein-S-isoprenylcysteine O-methyltransferase		YEASV		239
Protein-S-isoprenylcysteine O-methyltransferase		SACCK		151
+31

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L07952 Unassigned DNA Translation: AAA16520.1 L15442 Unassigned DNA Translation: AAA16840.1 U33007 Genomic DNA Translation: AAB64880.1 BK006938 Genomic DNA Translation: DAA12252.1
PIRⁱ	S37604
RefSeqⁱ	NP_010698.1, NM_001180718.1

3D structure databases

ProteinModelPortalⁱ	P32584
SMRⁱ	P32584
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32470, 19 interactors
DIPⁱ	DIP-2678N
IntActⁱ	P32584, 3 interactors
MINTⁱ	P32584
STRINGⁱ	4932.YDR410C

Chemistry databases

BindingDBⁱ	P32584
ChEMBLⁱ	CHEMBL4385

Proteomic databases

MaxQBⁱ	P32584
PaxDbⁱ	P32584
PRIDEⁱ	P32584

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDR410C; YDR410C; YDR410C
GeneIDⁱ	852019
KEGGⁱ	sce:YDR410C

Organism-specific databases

SGDⁱ	S000002818 STE14

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000017394
HOGENOMⁱ	HOG000213961
InParanoidⁱ	P32584
KOⁱ	K00587
OMAⁱ	LPFIRGY
OrthoDBⁱ	EOG092C5DVA

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29953-MONOMER
Reactomeⁱ	R-SCE-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P32584

PROⁱ

PR:P32584

Family and domain databases

InterProⁱ	View protein in InterPro IPR007269 ICMT_MeTrfase IPR025770 PPMT_MeTrfase
Pfamⁱ	View protein in Pfam PF04140 ICMT, 1 hit
PROSITEⁱ	View protein in PROSITE PS51564 SAM_ICMT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	STE14_YEAST
Accessionⁱ	P32584Primary (citable) accession number: P32584 Secondary accession number(s): D6VT42
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
	Last sequence update:	October 1, 1993
	Last modified:	November 7, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

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UniRef

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Supporting data

UniProtKB - P32584 (STE14_YEAST)

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Protein-S-isoprenylcysteine O-methyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Endoplasmic reticulum

Nucleus

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ