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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

STE14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functioni

GO - Biological processi

  • C-terminal protein methylation Source: SGD
  • peptide pheromone maturation Source: SGD
  • response to pheromone Source: UniProtKB-KW

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPheromone response
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER
ReactomeiR-SCE-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:STE14
Ordered Locus Names:YDR410C
ORF Names:D9461.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002818 STE14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23ExtracellularSequence analysisAdd BLAST23
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Topological domaini45 – 47CytoplasmicSequence analysis3
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 88ExtracellularSequence analysisAdd BLAST20
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110CytoplasmicSequence analysis1
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 182ExtracellularSequence analysisAdd BLAST51
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 239CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4385

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098991 – 239Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST239

Proteomic databases

MaxQBiP32584
PaxDbiP32584
PRIDEiP32584

Interactioni

Protein-protein interaction databases

BioGridi32470, 19 interactors
DIPiDIP-2678N
IntActiP32584, 3 interactors
MINTiP32584
STRINGi4932.YDR410C

Chemistry databases

BindingDBiP32584

Structurei

3D structure databases

ProteinModelPortaliP32584
SMRiP32584
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017394
HOGENOMiHOG000213961
InParanoidiP32584
KOiK00587
OMAiLPFIRGY
OrthoDBiEOG092C5DVA

Family and domain databases

InterProiView protein in InterPro
IPR007269 ICMT_MeTrfase
IPR025770 PPMT_MeTrfase
PfamiView protein in Pfam
PF04140 ICMT, 1 hit
PROSITEiView protein in PROSITE
PS51564 SAM_ICMT, 1 hit

Sequencei

Sequence statusi: Complete.

P32584-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN
60 70 80 90 100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL
110 120 130 140 150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI
160 170 180 190 200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF
210 220 230
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI
Length:239
Mass (Da):27,888
Last modified:October 1, 1993 - v1
Checksum:i189829AFD0BD6BD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA Translation: AAA16520.1
L15442 Unassigned DNA Translation: AAA16840.1
U33007 Genomic DNA Translation: AAB64880.1
BK006938 Genomic DNA Translation: DAA12252.1
PIRiS37604
RefSeqiNP_010698.1, NM_001180718.1

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C
GeneIDi852019
KEGGisce:YDR410C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA Translation: AAA16520.1
L15442 Unassigned DNA Translation: AAA16840.1
U33007 Genomic DNA Translation: AAB64880.1
BK006938 Genomic DNA Translation: DAA12252.1
PIRiS37604
RefSeqiNP_010698.1, NM_001180718.1

3D structure databases

ProteinModelPortaliP32584
SMRiP32584
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32470, 19 interactors
DIPiDIP-2678N
IntActiP32584, 3 interactors
MINTiP32584
STRINGi4932.YDR410C

Chemistry databases

BindingDBiP32584
ChEMBLiCHEMBL4385

Proteomic databases

MaxQBiP32584
PaxDbiP32584
PRIDEiP32584

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C
GeneIDi852019
KEGGisce:YDR410C

Organism-specific databases

SGDiS000002818 STE14

Phylogenomic databases

GeneTreeiENSGT00390000017394
HOGENOMiHOG000213961
InParanoidiP32584
KOiK00587
OMAiLPFIRGY
OrthoDBiEOG092C5DVA

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER
ReactomeiR-SCE-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation

Miscellaneous databases

PROiPR:P32584

Family and domain databases

InterProiView protein in InterPro
IPR007269 ICMT_MeTrfase
IPR025770 PPMT_MeTrfase
PfamiView protein in Pfam
PF04140 ICMT, 1 hit
PROSITEiView protein in PROSITE
PS51564 SAM_ICMT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTE14_YEAST
AccessioniPrimary (citable) accession number: P32584
Secondary accession number(s): D6VT42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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