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UniProtKB - P32584 (STE14_YEAST)
Protein
Protein-S-isoprenylcysteine O-methyltransferase
Gene
STE14
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins (PubMed:8289819, PubMed:11451995, PubMed:15611058).
Does not have a preference for the farnesyl or geranylgeranyl moieties in the model substrates N-acetyl-S-farnesyl-L-cysteine (AFC) and N-acetyl-S-geranylgeranyl-L-cysteine (AGGC) in vitro (PubMed:15611058).
3 PublicationsMiscellaneous
Present with 2690 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1001 Publication
Kineticsi
- KM=3 µM for N-acetyl-S-farnesyl-L-cysteine (AFC)1 Publication
- KM=3.2 µM for N-acetyl-S-geranylgeranyl-L-cysteine (AGGC)1 Publication
- Vmax=50782 pmol/min/mg enzyme for N-acetyl-S-farnesyl-L-cysteine (AFC)1 Publication
- Vmax=56667 pmol/min/mg enzyme for N-acetyl-S-geranylgeranyl-L-cysteine (AGGC)1 Publication
pH dependencei
Optimum pH is 7.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 167 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 209 | SubstrateBy similarity | 1 | |
Binding sitei | 213 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
GO - Biological processi
- C-terminal protein methylation Source: SGD
- peptide pheromone maturation Source: SGD
- response to pheromone Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Pheromone response |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
Reactomei | R-SCE-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)Short name: ICMT Alternative name(s): Isoprenylcysteine carboxylmethyltransferase Prenylated protein carboxyl methyltransferase Short name: PPMT Prenylcysteine carboxyl methyltransferase Short name: pcCMT |
Gene namesi | Name:STE14 Ordered Locus Names:YDR410C ORF Names:D9461.1 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002818, STE14 |
VEuPathDBi | FungiDB:YDR410C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: SGD
Nucleus
- nuclear inner membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 23 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 23 | |
Transmembranei | 24 – 44 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 45 – 47 | Lumenal2 Publications | 3 | |
Transmembranei | 48 – 68 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 88 | Cytoplasmic2 PublicationsAdd BLAST | 20 | |
Transmembranei | 89 – 109 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 110 | Lumenal2 Publications | 1 | |
Transmembranei | 111 – 131 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 132 – 175 | Cytoplasmic2 PublicationsAdd BLAST | 44 | |
Intramembranei | 176 – 206 | Helical1 PublicationAdd BLAST | 31 | |
Topological domaini | 207 – 239 | Cytoplasmic2 PublicationsAdd BLAST | 33 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | G → E: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 81 | L → F: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 132 | G → R: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 173 | P → L: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 213 | E → D: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 214 | E → D or Q: Abolishes function. 1 Publication | 1 | |
Mutagenesisi | 217 | L → S: Abolishes function. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4385 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209899 | 1 – 239 | Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST | 239 |
Proteomic databases
MaxQBi | P32584 |
PaxDbi | P32584 |
PRIDEi | P32584 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32470, 19 interactors |
DIPi | DIP-2678N |
IntActi | P32584, 3 interactors |
MINTi | P32584 |
STRINGi | 4932.YDR410C |
Chemistry databases
BindingDBi | P32584 |
Miscellaneous databases
RNActi | P32584, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 159 – 162 | S-adenosyl-L-methionine bindingBy similarity | 4 | |
Regioni | 172 – 175 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2628, Eukaryota |
GeneTreei | ENSGT00390000017394 |
HOGENOMi | CLU_065200_0_2_1 |
InParanoidi | P32584 |
OMAi | FFYWGLG |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
i Sequence
Sequence statusi: Complete.
P32584-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN
60 70 80 90 100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL
110 120 130 140 150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI
160 170 180 190 200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF
210 220 230
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07952 Unassigned DNA Translation: AAA16520.1 L15442 Unassigned DNA Translation: AAA16840.1 U33007 Genomic DNA Translation: AAB64880.1 BK006938 Genomic DNA Translation: DAA12252.1 |
PIRi | S37604 |
RefSeqi | NP_010698.1, NM_001180718.1 |
Genome annotation databases
EnsemblFungii | YDR410C_mRNA; YDR410C; YDR410C |
GeneIDi | 852019 |
KEGGi | sce:YDR410C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07952 Unassigned DNA Translation: AAA16520.1 L15442 Unassigned DNA Translation: AAA16840.1 U33007 Genomic DNA Translation: AAB64880.1 BK006938 Genomic DNA Translation: DAA12252.1 |
PIRi | S37604 |
RefSeqi | NP_010698.1, NM_001180718.1 |
3D structure databases
SMRi | P32584 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32470, 19 interactors |
DIPi | DIP-2678N |
IntActi | P32584, 3 interactors |
MINTi | P32584 |
STRINGi | 4932.YDR410C |
Chemistry databases
BindingDBi | P32584 |
ChEMBLi | CHEMBL4385 |
Proteomic databases
MaxQBi | P32584 |
PaxDbi | P32584 |
PRIDEi | P32584 |
Genome annotation databases
EnsemblFungii | YDR410C_mRNA; YDR410C; YDR410C |
GeneIDi | 852019 |
KEGGi | sce:YDR410C |
Organism-specific databases
SGDi | S000002818, STE14 |
VEuPathDBi | FungiDB:YDR410C |
Phylogenomic databases
eggNOGi | KOG2628, Eukaryota |
GeneTreei | ENSGT00390000017394 |
HOGENOMi | CLU_065200_0_2_1 |
InParanoidi | P32584 |
OMAi | FFYWGLG |
Enzyme and pathway databases
Reactomei | R-SCE-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation |
Miscellaneous databases
PROi | PR:P32584 |
RNActi | P32584, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STE14_YEAST | |
Accessioni | P32584Primary (citable) accession number: P32584 Secondary accession number(s): D6VT42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - SIMILARITY comments
Index of protein domains and families