UniProtKB - P32582 (CBS_YEAST)
Protein
Cystathionine beta-synthase
Gene
CYS4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 41900 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:4.2.1.22
Cofactori
: L-cysteine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Cystathionine beta-synthase (CYS4)
- Cystathionine gamma-lyase (CYS3)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 84 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 289 | Pyridoxal phosphateBy similarity | 1 |
GO - Molecular functioni
- cystathionine beta-synthase activity Source: SGD
- mRNA binding Source: SGD
- pyridoxal phosphate binding Source: GO_Central
GO - Biological processi
- cysteine biosynthetic process from serine Source: SGD
- cysteine biosynthetic process via cystathionine Source: SGD
- homocysteine metabolic process Source: GO_Central
- hydrogen sulfide biosynthetic process Source: SGD
- transsulfuration Source: SGD
- traversing start control point of mitotic cell cycle Source: SGD
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Cysteine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:YGR155W-MONOMER |
BRENDAi | 4.2.1.22, 984 |
Reactomei | R-SCE-1614603, Cysteine formation from homocysteine |
SABIO-RKi | P32582 |
UniPathwayi | UPA00136;UER00201 |
Names & Taxonomyi
Protein namesi | Recommended name: Cystathionine beta-synthase (EC:4.2.1.22)Alternative name(s): Beta-thionase Serine sulfhydrase Sulfur transfer protein 4 |
Gene namesi | Name:CYS4 Synonyms:STR4 Ordered Locus Names:YGR155W ORF Names:G6667 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003387, CYS4 |
VEuPathDBi | FungiDB:YGR155W |
Subcellular locationi
Mitochondrion
- mitochondrion Source: SGD
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000167135 | 1 – 507 | Cystathionine beta-synthaseAdd BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 134 | PhosphoserineCombined sources | 1 | |
Modified residuei | 350 | PhosphoserineCombined sources | 1 | |
Modified residuei | 424 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32582 |
PaxDbi | P32582 |
PRIDEi | P32582 |
PTM databases
iPTMneti | P32582 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33403, 192 interactors |
DIPi | DIP-1282N |
IntActi | P32582, 31 interactors |
MINTi | P32582 |
STRINGi | 4932.YGR155W |
Miscellaneous databases
RNActi | P32582, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32582 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 373 – 432 | CBSPROSITE-ProRule annotationAdd BLAST | 60 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 196 – 200 | Pyridoxal phosphate bindingBy similarity | 5 |
Sequence similaritiesi
Belongs to the cysteine synthase/cystathionine beta-synthase family.Curated
Keywords - Domaini
CBS domainPhylogenomic databases
eggNOGi | KOG1252, Eukaryota |
GeneTreei | ENSGT00510000047027 |
HOGENOMi | CLU_021018_0_0_1 |
InParanoidi | P32582 |
OMAi | WYVNQYD |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR005857, Cysta_beta_synth IPR001216, P-phosphate_BS IPR001926, PLP-dep IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00291, PALP, 1 hit |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR01137, cysta_beta, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 1 hit PS00901, CYS_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P32582-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKSEQQADS RHNVIDLVGN TPLIALKKLP KALGIKPQIY AKLELYNPGG
60 70 80 90 100
SIKDRIAKSM VEEAEASGRI HPSRSTLIEP TSGNTGIGLA LIGAIKGYRT
110 120 130 140 150
IITLPEKMSN EKVSVLKALG AEIIRTPTAA AWDSPESHIG VAKKLEKEIP
160 170 180 190 200
GAVILDQYNN MMNPEAHYFG TGREIQRQLE DLNLFDNLRA VVAGAGTGGT
210 220 230 240 250
ISGISKYLKE QNDKIQIVGA DPFGSILAQP ENLNKTDITD YKVEGIGYDF
260 270 280 290 300
VPQVLDRKLI DVWYKTDDKP SFKYARQLIS NEGVLVGGSS GSAFTAVVKY
310 320 330 340 350
CEDHPELTED DVIVAIFPDS IRSYLTKFVD DEWLKKNNLW DDDVLARFDS
360 370 380 390 400
SKLEASTTKY ADVFGNATVK DLHLKPVVSV KETAKVTDVI KILKDNGFDQ
410 420 430 440 450
LPVLTEDGKL SGLVTLSELL RKLSINNSNN DNTIKGKYLD FKKLNNFNDV
460 470 480 490 500
SSYNENKSGK KKFIKFDENS KLSDLNRFFE KNSSAVITDG LKPIHIVTKM
DLLSYLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | T → A in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 8 | A → T in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 63 | Missing in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 104 | L → W in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 129 | A → V in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 163 | N → T in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 407 | D → Y in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 436 – 437 | GK → VE in AAC37401 (PubMed:8022826).Curated | 2 | |
Sequence conflicti | 441 | F → V in AAC37401 (PubMed:8022826).Curated | 1 | |
Sequence conflicti | 481 | K → E in AAC37401 (PubMed:8022826).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 504 | S → N in strain: UCD932. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72922 Genomic DNA Translation: CAA51426.1 D16502 Genomic DNA Translation: BAA03952.1 L14578 Unassigned DNA Translation: AAC37401.1 DQ393806 Genomic DNA Translation: ABD57960.1 DQ393807 Genomic DNA Translation: ABD57961.1 DQ393808 Genomic DNA Translation: ABD57962.1 DQ393809 Genomic DNA Translation: ABD57963.1 X85807 Genomic DNA Translation: CAA59812.1 Z72940 Genomic DNA Translation: CAA97169.1 BK006941 Genomic DNA Translation: DAA08246.1 |
PIRi | A48661 |
RefSeqi | NP_011671.3, NM_001181284.3 |
Genome annotation databases
EnsemblFungii | YGR155W_mRNA; YGR155W; YGR155W |
GeneIDi | 853059 |
KEGGi | sce:YGR155W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72922 Genomic DNA Translation: CAA51426.1 D16502 Genomic DNA Translation: BAA03952.1 L14578 Unassigned DNA Translation: AAC37401.1 DQ393806 Genomic DNA Translation: ABD57960.1 DQ393807 Genomic DNA Translation: ABD57961.1 DQ393808 Genomic DNA Translation: ABD57962.1 DQ393809 Genomic DNA Translation: ABD57963.1 X85807 Genomic DNA Translation: CAA59812.1 Z72940 Genomic DNA Translation: CAA97169.1 BK006941 Genomic DNA Translation: DAA08246.1 |
PIRi | A48661 |
RefSeqi | NP_011671.3, NM_001181284.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6C2H | X-ray | 1.49 | A | 1-353 | [»] | |
6C2Q | X-ray | 2.17 | A | 1-353 | [»] | |
6C2Z | X-ray | 1.37 | A | 1-353 | [»] | |
6C4P | X-ray | 2.30 | A | 1-353 | [»] | |
SMRi | P32582 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33403, 192 interactors |
DIPi | DIP-1282N |
IntActi | P32582, 31 interactors |
MINTi | P32582 |
STRINGi | 4932.YGR155W |
PTM databases
iPTMneti | P32582 |
Proteomic databases
MaxQBi | P32582 |
PaxDbi | P32582 |
PRIDEi | P32582 |
Genome annotation databases
EnsemblFungii | YGR155W_mRNA; YGR155W; YGR155W |
GeneIDi | 853059 |
KEGGi | sce:YGR155W |
Organism-specific databases
SGDi | S000003387, CYS4 |
VEuPathDBi | FungiDB:YGR155W |
Phylogenomic databases
eggNOGi | KOG1252, Eukaryota |
GeneTreei | ENSGT00510000047027 |
HOGENOMi | CLU_021018_0_0_1 |
InParanoidi | P32582 |
OMAi | WYVNQYD |
Enzyme and pathway databases
UniPathwayi | UPA00136;UER00201 |
BioCyci | MetaCyc:YGR155W-MONOMER |
BRENDAi | 4.2.1.22, 984 |
Reactomei | R-SCE-1614603, Cysteine formation from homocysteine |
SABIO-RKi | P32582 |
Miscellaneous databases
PROi | PR:P32582 |
RNActi | P32582, protein |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR005857, Cysta_beta_synth IPR001216, P-phosphate_BS IPR001926, PLP-dep IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00571, CBS, 2 hits PF00291, PALP, 1 hit |
SMARTi | View protein in SMART SM00116, CBS, 2 hits |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR01137, cysta_beta, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 1 hit PS00901, CYS_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBS_YEAST | |
Accessioni | P32582Primary (citable) accession number: P32582 Secondary accession number(s): D6VUT5 Q27JK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names