We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P32565 (RPN2_YEAST)
Protein
26S proteasome regulatory subunit RPN2
Gene
RPN2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
1 PublicationMiscellaneous
Present with 4750 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- enzyme regulator activity Source: InterPro
- ubiquitin protein ligase binding Source: SGD
GO - Biological processi
- proteasome assembly Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: ComplexPortal
- regulation of protein catabolic process Source: InterPro
- ubiquitin-dependent protein catabolic process Source: SGD
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-382556, ABC-family proteins mediated transport R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5687128, MAPK6/MAPK4 signaling R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation R-SCE-68949, Orc1 removal from chromatin R-SCE-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SCE-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-SCE-75815, Ubiquitin-dependent degradation of Cyclin D R-SCE-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SCE-8948751, Regulation of PTEN stability and activity R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit RPN2 |
Gene namesi | Name:RPN2 Synonyms:SEN3 Ordered Locus Names:YIL075C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001337, RPN2 |
VEuPathDBi | FungiDB:YIL075C |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- proteasome complex Source: ComplexPortal
- proteasome regulatory particle, base subcomplex Source: SGD
- proteasome storage granule Source: SGD
Keywords - Cellular componenti
ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000173808 | 2 – 945 | 26S proteasome regulatory subunit RPN2Add BLAST | 944 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 801 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 932 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
N-acetylated by NAT1.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P32565 |
PaxDbi | P32565 |
PRIDEi | P32565 |
PTM databases
iPTMneti | P32565 |
Interactioni
Subunit structurei
Interacts with UBR1.
1 PublicationBinary interactionsi
GO - Molecular functioni
- ubiquitin protein ligase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 34917, 144 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-4638N |
IntActi | P32565, 84 interactors |
MINTi | P32565 |
STRINGi | 4932.YIL075C |
Miscellaneous databases
RNActi | P32565, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32565 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 366 – 399 | PC 1Add BLAST | 34 | |
Repeati | 403 – 440 | PC 2Add BLAST | 38 | |
Repeati | 445 – 479 | PC 3Add BLAST | 35 | |
Repeati | 480 – 514 | PC 4Add BLAST | 35 | |
Repeati | 516 – 549 | PC 5Add BLAST | 34 | |
Repeati | 550 – 585 | PC 6Add BLAST | 36 | |
Repeati | 586 – 618 | PC 7Add BLAST | 33 | |
Repeati | 620 – 654 | PC 8Add BLAST | 35 | |
Repeati | 655 – 692 | PC 9Add BLAST | 38 | |
Repeati | 698 – 734 | PC 10Add BLAST | 37 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 810 – 851 | DisorderedSequence analysisAdd BLAST | 42 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 818 – 851 | Basic and acidic residuesSequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the proteasome subunit S1 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2062, Eukaryota |
GeneTreei | ENSGT00940000153386 |
HOGENOMi | CLU_002323_0_0_1 |
InParanoidi | P32565 |
OMAi | CMDIVQE |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR016642, 26S_Psome_Rpn2 IPR011989, ARM-like IPR016024, ARM-type_fold IPR002015, Proteasome/cyclosome_rpt IPR035266, PSMD1 IPR040623, RPN2_C |
PANTHERi | PTHR10943:SF2, PTHR10943:SF2, 1 hit |
Pfami | View protein in Pfam PF01851, PC_rep, 2 hits PF18004, RPN2_C, 1 hit |
PIRSFi | PIRSF015947, 26S_Psome_Rpn2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32565-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLTTAAPLL ALLRENQDSV KTYALESINN VVDQLWSEIS NELPDIEALY
60 70 80 90 100
DDDTFSDREM AALIASKVYY NLGEYESAVK YALAAKDRFD IDEKSQFVET
110 120 130 140 150
IVSKSIEMYV QEASKQYTKD EQFYTKDIID PKLTSIFERM IEKCLKASEL
160 170 180 190 200
KLALGIALEG YRLDIIESAL KSKLDQDSTS ENVKIINYLL TLAITTVTNS
210 220 230 240 250
KFRSSILRKS FDFLMNMPNC DYLTLNKVVV NLNDAGLALQ LFKKLKEEND
260 270 280 290 300
EGLSAQIAFD LVSSASQQLL EILVTELTAQ GYDPALLNIL SGLPTCDYYN
310 320 330 340 350
TFLLNNKNID IGLLNKSKSS LDGKFSLFHT AVSVANGFMH AGTTDNSFIK
360 370 380 390 400
ANLPWLGKAQ NWAKFTATAS LGVIHKGNLL EGKKVMAPYL PGSRASSRFI
410 420 430 440 450
KGGSLYGLGL IYAGFGRDTT DYLKNIIVEN SGTSGDEDVD VLLHGASLGI
460 470 480 490 500
GLAAMGSANI EVYEALKEVL YNDSATSGEA AALGMGLCML GTGKPEAIHD
510 520 530 540 550
MFTYSQETQH GNITRGLAVG LALINYGRQE LADDLITKML ASDESLLRYG
560 570 580 590 600
GAFTIALAYA GTGNNSAVKR LLHVAVSDSN DDVRRAAVIA LGFVLLRDYT
610 620 630 640 650
TVPRIVQLLS KSHNAHVRCG TAFALGIACA GKGLQSAIDV LDPLTKDPVD
660 670 680 690 700
FVRQAAMIAL SMILIQQTEK LNPQVADINK NFLSVITNKH QEGLAKFGAC
710 720 730 740 750
VAQGIMNAGG RNVTIQLENA DTGTLDTKSV VGLVMFSQFW YWFPLAHFLS
760 770 780 790 800
LSFTPTTVIG IRGSDQAIPK FQMNCYAKED AFSYPRMYEE ASGKEVEKVA
810 820 830 840 850
TAVLSTTARA KARAKKTKKE KGPNEEEKKK EHEEKEKERE TNKKGIKETK
860 870 880 890 900
ENDEEFYKNK YSSKPYKVDN MTRILPQQSR YISFIKDDRF VPVRKFKGNN
910 920 930 940
GVVVLRDREP KEPVALIETV RQMKDVNAPL PTPFKVDDNV DFPSA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 333 – 334 | SV → RL in AAA87613 (PubMed:7565784).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06321 Genomic DNA Translation: AAA87613.1 Z37997 Genomic DNA Translation: CAA86095.1 BK006942 Genomic DNA Translation: DAA08475.1 |
PIRi | S48369 |
RefSeqi | NP_012190.1, NM_001179425.1 |
Genome annotation databases
EnsemblFungii | YIL075C_mRNA; YIL075C; YIL075C |
GeneIDi | 854735 |
KEGGi | sce:YIL075C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06321 Genomic DNA Translation: AAA87613.1 Z37997 Genomic DNA Translation: CAA86095.1 BK006942 Genomic DNA Translation: DAA08475.1 |
PIRi | S48369 |
RefSeqi | NP_012190.1, NM_001179425.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCO | electron microscopy | 4.80 | N | 1-945 | [»] | |
3JCP | electron microscopy | 4.60 | N | 1-945 | [»] | |
4ADY | X-ray | 2.70 | A/B | 2-945 | [»] | |
4CR2 | electron microscopy | 7.70 | N | 1-945 | [»] | |
4CR3 | electron microscopy | 9.30 | N | 1-945 | [»] | |
4CR4 | electron microscopy | 8.80 | N | 1-945 | [»] | |
5A5B | electron microscopy | 9.50 | N | 1-945 | [»] | |
5MPB | electron microscopy | 7.80 | N | 1-945 | [»] | |
5MPC | electron microscopy | 7.70 | N | 1-945 | [»] | |
5MPD | electron microscopy | 4.10 | N | 1-945 | [»] | |
5MPE | electron microscopy | 4.50 | N | 1-945 | [»] | |
5WVI | electron microscopy | 6.30 | N | 1-945 | [»] | |
5WVK | electron microscopy | 4.20 | N | 1-945 | [»] | |
6FVT | electron microscopy | 4.10 | N | 4-925 | [»] | |
6FVU | electron microscopy | 4.50 | N | 4-925 | [»] | |
6FVV | electron microscopy | 5.40 | N | 4-925 | [»] | |
6FVW | electron microscopy | 4.50 | N | 4-925 | [»] | |
6FVX | electron microscopy | 4.90 | N | 4-925 | [»] | |
6FVY | electron microscopy | 6.10 | N | 4-925 | [»] | |
6J2C | electron microscopy | 7.00 | N | 1-945 | [»] | |
6J2N | electron microscopy | 7.50 | N | 1-945 | [»] | |
6J2Q | electron microscopy | 3.80 | N | 1-945 | [»] | |
6J2X | electron microscopy | 3.80 | N | 1-945 | [»] | |
6J30 | electron microscopy | 4.50 | N | 1-945 | [»] | |
SMRi | P32565 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34917, 144 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-4638N |
IntActi | P32565, 84 interactors |
MINTi | P32565 |
STRINGi | 4932.YIL075C |
PTM databases
iPTMneti | P32565 |
Proteomic databases
MaxQBi | P32565 |
PaxDbi | P32565 |
PRIDEi | P32565 |
Genome annotation databases
EnsemblFungii | YIL075C_mRNA; YIL075C; YIL075C |
GeneIDi | 854735 |
KEGGi | sce:YIL075C |
Organism-specific databases
SGDi | S000001337, RPN2 |
VEuPathDBi | FungiDB:YIL075C |
Phylogenomic databases
eggNOGi | KOG2062, Eukaryota |
GeneTreei | ENSGT00940000153386 |
HOGENOMi | CLU_002323_0_0_1 |
InParanoidi | P32565 |
OMAi | CMDIVQE |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-382556, ABC-family proteins mediated transport R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5687128, MAPK6/MAPK4 signaling R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation R-SCE-68949, Orc1 removal from chromatin R-SCE-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SCE-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-SCE-75815, Ubiquitin-dependent degradation of Cyclin D R-SCE-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SCE-8948751, Regulation of PTEN stability and activity R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P32565 |
RNActi | P32565, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR016642, 26S_Psome_Rpn2 IPR011989, ARM-like IPR016024, ARM-type_fold IPR002015, Proteasome/cyclosome_rpt IPR035266, PSMD1 IPR040623, RPN2_C |
PANTHERi | PTHR10943:SF2, PTHR10943:SF2, 1 hit |
Pfami | View protein in Pfam PF01851, PC_rep, 2 hits PF18004, RPN2_C, 1 hit |
PIRSFi | PIRSF015947, 26S_Psome_Rpn2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPN2_YEAST | |
Accessioni | P32565Primary (citable) accession number: P32565 Secondary accession number(s): D6VVK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families