We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P32562 (CDC5_YEAST)
Protein
Cell cycle serine/threonine-protein kinase CDC5/MSD2
Gene
CDC5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Protein kinase required for the cell cycle where it is involved in mitotic exit. A component of the fear (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Phosphorylates SCC1/MCD1 and NET1.
3 PublicationsMiscellaneous
Present with 1480 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 110 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 204 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 88 – 96 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- centromeric DNA binding Source: SGD
- phosphoprotein binding Source: SGD
- protein-containing complex binding Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: SGD
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell division Source: UniProtKB-KW
- exit from mitosis Source: SGD
- meiotic spindle assembly Source: SGD
- mitotic cell cycle Source: GO_Central
- negative regulation of protein localization to nucleolus Source: SGD
- positive regulation of cohesin unloading Source: SGD
- positive regulation of mitotic spindle pole body separation Source: SGD
- positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly Source: SGD
- positive regulation of Rho protein signal transduction Source: SGD
- protein phosphorylation Source: SGD
- regulation of cytokinesis Source: GO_Central
- regulation of nucleus organization Source: SGD
- regulation of protein localization to mitotic spindle pole body Source: SGD
- resolution of meiotic recombination intermediates Source: SGD
- synaptonemal complex disassembly Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.21, 984 |
Reactomei | R-SCE-156711, Polo-like kinase mediated events R-SCE-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-SCE-2500257, Resolution of Sister Chromatid Cohesion R-SCE-2565942, Regulation of PLK1 Activity at G2/M Transition R-SCE-6804115, TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CDC5 Synonyms:MSD2, PKX2 Ordered Locus Names:YMR001C ORF Names:YM8270.03C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004603, CDC5 |
VEuPathDBi | FungiDB:YMR001C |
Subcellular locationi
Cytoskeleton
- spindle pole Source: SGD
- spindle pole body Source: GO_Central
Nucleus
- nucleus Source: SGD
Other locations
- cellular bud neck Source: SGD
- cytoplasm Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085762 | 1 – 705 | Cell cycle serine/threonine-protein kinase CDC5/MSD2Add BLAST | 705 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 419 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32562 |
PaxDbi | P32562 |
PRIDEi | P32562 |
PTM databases
iPTMneti | P32562 |
Interactioni
Subunit structurei
Interacts with CDC48; the interaction is likely to result in CDC5 degradation.
1 PublicationBinary interactionsi
P32562
GO - Molecular functioni
- phosphoprotein binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35171, 219 interactors |
DIPi | DIP-2321N |
IntActi | P32562, 65 interactors |
MINTi | P32562 |
STRINGi | 4932.YMR001C |
Miscellaneous databases
RNActi | P32562, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32562 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 337 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 | |
Domaini | 520 – 587 | POLO box 1PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 619 – 692 | POLO box 2PROSITE-ProRule annotationAdd BLAST | 74 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 63 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0575, Eukaryota |
GeneTreei | ENSGT00940000157752 |
HOGENOMi | CLU_000288_46_2_1 |
InParanoidi | P32562 |
OMAi | YIMDRLY |
Family and domain databases
CDDi | cd13118, POLO_box_1, 1 hit cd13117, POLO_box_2, 1 hit |
Gene3Di | 3.30.1120.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR033701, POLO_box_1 IPR033695, POLO_box_2 IPR000959, POLO_box_dom IPR036947, POLO_box_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00659, POLO_box, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50078, POLO_BOX, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P32562-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND
60 70 80 90 100
HHHQPAQKKK REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK
110 120 130 140 150
DDSGEIFAAK TVAKASIKSE KTRKKLLSEI QIHKSMSHPN IVQFIDCFED
160 170 180 190 200
DSNVYILLEI CPNGSLMELL KRRKVLTEPE VRFFTTQICG AIKYMHSRRV
210 220 230 240 250
IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC GTPNYIAPEV
260 270 280 290 300
LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF
310 320 330 340 350
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM
360 370 380 390 400
SEAPNFEDIP EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS
410 420 430 440 450
IDKLEEYHQN RPFLPHSLSP GGTKQKYKEV VDIEAQRRLN DLAREARIRR
460 470 480 490 500
AQQAVLRKEL IATSTNVIKS EISLRILASE CHLTLNGIVE AEAQYKMGGL
510 520 530 540 550
PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN NGTTVLRLAD
560 570 580 590 600
AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV
610 620 630 640 650
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG
660 670 680 690 700
KLVTYISPSH ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST
IVTVD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84220 Unassigned DNA Translation: AAA02576.1 Z48613 Genomic DNA Translation: CAA88516.1 BK006946 Genomic DNA Translation: DAA09899.1 |
PIRi | A48144 |
RefSeqi | NP_013714.1, NM_001182497.1 |
Genome annotation databases
EnsemblFungii | YMR001C_mRNA; YMR001C; YMR001C |
GeneIDi | 855013 |
KEGGi | sce:YMR001C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84220 Unassigned DNA Translation: AAA02576.1 Z48613 Genomic DNA Translation: CAA88516.1 BK006946 Genomic DNA Translation: DAA09899.1 |
PIRi | A48144 |
RefSeqi | NP_013714.1, NM_001182497.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6MF4 | X-ray | 1.80 | A | 418-705 | [»] | |
6MF5 | X-ray | 2.70 | A/B | 418-705 | [»] | |
6MF6 | X-ray | 3.40 | A/B | 418-705 | [»] | |
SMRi | P32562 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35171, 219 interactors |
DIPi | DIP-2321N |
IntActi | P32562, 65 interactors |
MINTi | P32562 |
STRINGi | 4932.YMR001C |
PTM databases
iPTMneti | P32562 |
Proteomic databases
MaxQBi | P32562 |
PaxDbi | P32562 |
PRIDEi | P32562 |
Genome annotation databases
EnsemblFungii | YMR001C_mRNA; YMR001C; YMR001C |
GeneIDi | 855013 |
KEGGi | sce:YMR001C |
Organism-specific databases
SGDi | S000004603, CDC5 |
VEuPathDBi | FungiDB:YMR001C |
Phylogenomic databases
eggNOGi | KOG0575, Eukaryota |
GeneTreei | ENSGT00940000157752 |
HOGENOMi | CLU_000288_46_2_1 |
InParanoidi | P32562 |
OMAi | YIMDRLY |
Enzyme and pathway databases
BRENDAi | 2.7.11.21, 984 |
Reactomei | R-SCE-156711, Polo-like kinase mediated events R-SCE-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-SCE-2500257, Resolution of Sister Chromatid Cohesion R-SCE-2565942, Regulation of PLK1 Activity at G2/M Transition R-SCE-6804115, TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain |
Miscellaneous databases
PROi | PR:P32562 |
RNActi | P32562, protein |
Family and domain databases
CDDi | cd13118, POLO_box_1, 1 hit cd13117, POLO_box_2, 1 hit |
Gene3Di | 3.30.1120.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR033701, POLO_box_1 IPR033695, POLO_box_2 IPR000959, POLO_box_dom IPR036947, POLO_box_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00659, POLO_box, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50078, POLO_BOX, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC5_YEAST | |
Accessioni | P32562Primary (citable) accession number: P32562 Secondary accession number(s): D6VZH5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 208 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families