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UniProtKB - P32528 (DUR1_YEAST)
Protein
Urea amidolyase
Gene
DUR1,2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Hydrolysis of urea to ammonia and CO2.
Miscellaneous
Present with 952 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
: urea degradation Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route). This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route), the pathway urea degradation and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 747 | ATPBy similarity | 1 | |
Binding sitei | 830 | ATPBy similarity | 1 | |
Binding sitei | 865 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 122 – 129 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- allophanate hydrolase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: InterPro
- urea carboxylase activity Source: SGD
GO - Biological processi
- allantoin catabolic process Source: SGD
- urea catabolic process Source: SGD
Keywordsi
Molecular function | Hydrolase, Ligase, Multifunctional enzyme |
Ligand | ATP-binding, Biotin, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.5.1.54, 984 6.3.4.6, 984 |
UniPathwayi | UPA00258;UER00371 UPA00258;UER00372 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DUR1,2 Ordered Locus Names:YBR208C ORF Names:YBR1448 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000412, DUR1,2 |
VEuPathDBi | FungiDB:YBR208C |
Subcellular locationi
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146832 | 1 – 1835 | Urea amidolyaseAdd BLAST | 1835 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 803 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1798 | N6-biotinyllysinePROSITE-ProRule annotationBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32528 |
PaxDbi | P32528 |
PRIDEi | P32528 |
PTM databases
iPTMneti | P32528 |
Expressioni
Inductioni
By allophanate or its non-metabolized analog oxalurate. Repressed in the presence of readily used nitrogen sources.
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 32904, 40 interactors |
DIPi | DIP-6296N |
IntActi | P32528, 9 interactors |
STRINGi | 4932.YBR208C |
Miscellaneous databases
RNActi | P32528, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 632 – 1075 | Biotin carboxylationAdd BLAST | 444 | |
Domaini | 751 – 948 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 198 | |
Domaini | 1754 – 1832 | Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST | 79 |
Phylogenomic databases
eggNOGi | KOG0238, Eukaryota KOG1211, Eukaryota |
HOGENOMi | CLU_002162_4_1_1 |
InParanoidi | P32528 |
OMAi | HDCEYAI |
Family and domain databases
Gene3Di | 2.40.100.10, 2 hits 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR014085, Allophanate_hydrolase IPR023631, Amidase_dom IPR036928, AS_sf IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR003778, CT_A_B IPR003833, CT_C_D IPR029000, Cyclophilin-like_dom_sf IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif IPR014084, Urea_COase |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF02626, CT_A_B, 1 hit PF02682, CT_C_D, 1 hit |
SMARTi | View protein in SMART SM00796, AHS1, 1 hit SM00797, AHS2, 1 hit SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF50891, SSF50891, 2 hits SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR02713, allophanate_hyd, 1 hit TIGR00724, urea_amlyse_rel, 1 hit TIGR02712, urea_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32528-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVSSDTTAE ISLGWSIQDW IDFHKSSSSQ ASLRLLESLL DSQNVAPVDN
60 70 80 90 100
AWISLISKEN LLHQFQILKS RENKETLPLY GVPIAVKDNI DVRGLPTTAA
110 120 130 140 150
CPSFAYEPSK DSKVVELLRN AGAIIVGKTN LDQFATGLVG TRSPYGKTPC
160 170 180 190 200
AFSKEHVSGG SSAGSASVVA RGIVPIALGT DTAGSGRVPA ALNNLIGLKP
210 220 230 240 250
TKGVFSCQGV VPACKSLDCV SIFALNLSDA ERCFRIMCQP DPDNDEYSRP
260 270 280 290 300
YVSNPLKKFS SNVTIAIPKN IPWYGETKNP VLFSNAVENL SRTGANVIEI
310 320 330 340 350
DFEPLLELAR CLYEGTWVAE RYQAIQSFLD SKPPKESLDP TVISIIEGAK
360 370 380 390 400
KYSAVDCFSF EYKRQGILQK VRRLLESVDV LCVPTCPLNP TMQQVADEPV
410 420 430 440 450
LVNSRQGTWT NFVNLADLAA LAVPAGFRDD GLPNGITLIG KKFTDYALLE
460 470 480 490 500
LANRYFQNIF PNGSRTYGTF TSSSVKPAND QLVGPDYDPS TSIKLAVVGA
510 520 530 540 550
HLKGLPLHWQ LEKVNATYLC TTKTSKAYQL FALPKNGPVL KPGLRRVQDS
560 570 580 590 600
NGSQIELEVY SVPKELFGAF ISMVPEPLGI GSVELESGEW IKSFICEESG
610 620 630 640 650
YKAKGTVDIT KYGGFRAYFE MLKKKESQKK KLFDTVLIAN RGEIAVRIIK
660 670 680 690 700
TLKKLGIRSV AVYSDPDKYS QHVTDADVSV PLHGTTAAQT YLDMNKIIDA
710 720 730 740 750
AKQTNAQAII PGYGFLSENA DFSDACTSAG ITFVGPSGDI IRGLGLKHSA
760 770 780 790 800
RQIAQKAGVP LVPGSLLITS VEEAKKVAAE LEYPVMVKST AGGGGIGLQK
810 820 830 840 850
VDSEEDIEHI FETVKHQGET FFGDAGVFLE RFIENARHVE VQLMGDGFGK
860 870 880 890 900
AIALGERDCS LQRRNQKVIE ETPAPNLPEK TRLALRKAAE SLGSLLNYKC
910 920 930 940 950
AGTVEFIYDE KKDEFYFLEV NTRLQVEHPI TEMVTGLDLV EWMIRIAAND
960 970 980 990 1000
APDFDSTKVE VNGVSMEARL YAENPLKNFR PSPGLLVDVK FPDWARVDTW
1010 1020 1030 1040 1050
VKKGTNISPE YDPTLAKIIV HGKDRDDAIS KLNQALEETK VYGCITNIDY
1060 1070 1080 1090 1100
LKSIITSDFF AKAKVSTNIL NSYQYEPTAI EITLPGAHTS IQDYPGRVGY
1110 1120 1130 1140 1150
WRIGVPPSGP MDAYSFRLAN RIVGNDYRTP AIEVTLTGPS IVFHCETVIA
1160 1170 1180 1190 1200
ITGGTALCTL DGQEIPQHKP VEVKRGSTLS IGKLTSGCRA YLGIRGGIDV
1210 1220 1230 1240 1250
PKYLGSYSTF TLGNVGGYNG RVLKLGDVLF LPSNEENKSV ECLPQNIPQS
1260 1270 1280 1290 1300
LIPQISETKE WRIGVTCGPH GSPDFFKPES IEEFFSEKWK VHYNSNRFGV
1310 1320 1330 1340 1350
RLIGPKPKWA RSNGGEGGMH PSNTHDYVYS LGAINFTGDE PVIITCDGPS
1360 1370 1380 1390 1400
LGGFVCQAVV PEAELWKVGQ VKPGDSIQFV PLSYESSRSL KESQDVAIKS
1410 1420 1430 1440 1450
LDGTKLRRLD SVSILPSFET PILAQMEKVN ELSPKVVYRQ AGDRYVLVEY
1460 1470 1480 1490 1500
GDNEMNFNIS YRIECLISLV KKNKTIGIVE MSQGVRSVLI EFDGYKVTQK
1510 1520 1530 1540 1550
ELLKVLVAYE TEIQFDENWK ITSNIIRLPM AFEDSKTLAC VQRYQETIRS
1560 1570 1580 1590 1600
SAPWLPNNVD FIANVNGISR NEVYDMLYSA RFMVLGLGDV FLGSPCAVPL
1610 1620 1630 1640 1650
DPRHRFLGSK YNPSRTYTER GAVGIGGMYM CIYAANSPGG YQLVGRTIPI
1660 1670 1680 1690 1700
WDKLCLAASS EVPWLMNPFD QVEFYPVSEE DLDKMTEDCD NGVYKVNIEK
1710 1720 1730 1740 1750
SVFDHQEYLR WINANKDSIT AFQEGQLGER AEEFAKLIQN ANSELKESVT
1760 1770 1780 1790 1800
VKPDEEEDFP EGAEIVYSEY SGRFWKSIAS VGDVIEAGQG LLIIEAMKAE
1810 1820 1830
MIISAPKSGK IIKICHGNGD MVDSGDIVAV IETLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 96 | P → R in AAC41643 (PubMed:1802034).Curated | 1 | |
Sequence conflicti | 256 – 258 | LKK → KKN in AAC41643 (PubMed:1802034).Curated | 3 | |
Sequence conflicti | 459 | I → M in AAC41643 (PubMed:1802034).Curated | 1 | |
Sequence conflicti | 830 | E → K in AAC41643 (PubMed:1802034).Curated | 1 | |
Sequence conflicti | 1395 | D → E in AAC41643 (PubMed:1802034).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64926 Genomic DNA Translation: AAC41643.1 Z36077 Genomic DNA Translation: CAA85172.1 Z21487 Genomic DNA Translation: CAA79695.1 BK006936 Genomic DNA Translation: DAA07325.1 |
PIRi | S46082 |
RefSeqi | NP_009767.1, NM_001178556.1 |
Genome annotation databases
EnsemblFungii | YBR208C_mRNA; YBR208C; YBR208C |
GeneIDi | 852507 |
KEGGi | sce:YBR208C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64926 Genomic DNA Translation: AAC41643.1 Z36077 Genomic DNA Translation: CAA85172.1 Z21487 Genomic DNA Translation: CAA79695.1 BK006936 Genomic DNA Translation: DAA07325.1 |
PIRi | S46082 |
RefSeqi | NP_009767.1, NM_001178556.1 |
3D structure databases
AlphaFoldDBi | P32528 |
SMRi | P32528 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32904, 40 interactors |
DIPi | DIP-6296N |
IntActi | P32528, 9 interactors |
STRINGi | 4932.YBR208C |
PTM databases
iPTMneti | P32528 |
Proteomic databases
MaxQBi | P32528 |
PaxDbi | P32528 |
PRIDEi | P32528 |
Genome annotation databases
EnsemblFungii | YBR208C_mRNA; YBR208C; YBR208C |
GeneIDi | 852507 |
KEGGi | sce:YBR208C |
Organism-specific databases
SGDi | S000000412, DUR1,2 |
VEuPathDBi | FungiDB:YBR208C |
Phylogenomic databases
eggNOGi | KOG0238, Eukaryota KOG1211, Eukaryota |
HOGENOMi | CLU_002162_4_1_1 |
InParanoidi | P32528 |
OMAi | HDCEYAI |
Enzyme and pathway databases
UniPathwayi | UPA00258;UER00371 UPA00258;UER00372 |
BRENDAi | 3.5.1.54, 984 6.3.4.6, 984 |
Miscellaneous databases
PROi | PR:P32528 |
RNActi | P32528, protein |
Family and domain databases
Gene3Di | 2.40.100.10, 2 hits 3.90.1300.10, 1 hit |
InterProi | View protein in InterPro IPR014085, Allophanate_hydrolase IPR023631, Amidase_dom IPR036928, AS_sf IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR003778, CT_A_B IPR003833, CT_C_D IPR029000, Cyclophilin-like_dom_sf IPR016185, PreATP-grasp_dom_sf IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif IPR014084, Urea_COase |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF02626, CT_A_B, 1 hit PF02682, CT_C_D, 1 hit |
SMARTi | View protein in SMART SM00796, AHS1, 1 hit SM00797, AHS2, 1 hit SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF50891, SSF50891, 2 hits SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR02713, allophanate_hyd, 1 hit TIGR00724, urea_amlyse_rel, 1 hit TIGR02712, urea_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS00866, CPSASE_1, 1 hit PS00867, CPSASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DUR1_YEAST | |
Accessioni | P32528Primary (citable) accession number: P32528 Secondary accession number(s): D6VQK5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 204 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways