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Protein

Urea amidolyase

Gene

DUR1,2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of urea to ammonia and CO2.

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate.
Urea-1-carboxylate + H2O = 2 CO2 + 2 NH3.

Cofactori

Pathwayi: urea degradation

This protein is involved in step 1 and 2 of the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Urea amidolyase (DUR1,2)
  2. Urea amidolyase (DUR1,2)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (allophanate route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei747ATPBy similarity1
Binding sitei830ATPBy similarity1
Binding sitei865ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi122 – 129ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • allophanate hydrolase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: InterPro
  • urea carboxylase activity Source: SGD

GO - Biological processi

  • allantoin catabolic process Source: SGD
  • arginine metabolic process Source: UniProtKB-KW
  • urea catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase, Ligase, Multifunctional enzyme
Biological processArginine metabolism
LigandATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YBR208C-MONOMER
YEAST:YBR208C-MONOMER
BRENDAi6.3.4.6 984
ReactomeiR-SCE-8951664 Neddylation
UniPathwayiUPA00258; UER00371
UPA00258; UER00372

Names & Taxonomyi

Protein namesi
Recommended name:
Urea amidolyase
Including the following 2 domains:
Urea carboxylase (EC:6.3.4.6)
Allophanate hydrolase (EC:3.5.1.54)
Gene namesi
Name:DUR1,2
Ordered Locus Names:YBR208C
ORF Names:YBR1448
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR208C
SGDiS000000412 DUR1,2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001468321 – 1835Urea amidolyaseAdd BLAST1835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei803PhosphoserineCombined sources1
Modified residuei1798N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32528
PaxDbiP32528
PRIDEiP32528

PTM databases

iPTMnetiP32528

Expressioni

Inductioni

By allophanate or its non-metabolized analog oxalurate. Repressed in the presence of readily used nitrogen sources.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi32904, 39 interactors
DIPiDIP-6296N
IntActiP32528, 13 interactors
STRINGi4932.YBR208C

Structurei

3D structure databases

ProteinModelPortaliP32528
SMRiP32528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini632 – 1075Biotin carboxylationAdd BLAST444
Domaini751 – 948ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini1754 – 1832Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

GeneTreeiENSGT00550000074675
HOGENOMiHOG000251581
InParanoidiP32528
KOiK14541
OMAiHYNSNRL
OrthoDBiEOG092C0324

Family and domain databases

Gene3Di2.40.100.10, 2 hits
3.90.1300.10, 2 hits
InterProiView protein in InterPro
IPR014085 Allophanate_hydrolase
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR003778 CT_A_B
IPR003833 CT_C_D
IPR029000 Cyclophilin-like_dom_sf
IPR016185 PreATP-grasp_dom_sf
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
IPR014084 Urea_COase
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF02626 CT_A_B, 1 hit
PF02682 CT_C_D, 1 hit
SMARTiView protein in SMART
SM00796 AHS1, 1 hit
SM00797 AHS2, 1 hit
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF50891 SSF50891, 2 hits
SSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR02713 allophanate_hyd, 1 hit
TIGR00724 urea_amlyse_rel, 1 hit
TIGR02712 urea_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00866 CPSASE_1, 1 hit
PS00867 CPSASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVSSDTTAE ISLGWSIQDW IDFHKSSSSQ ASLRLLESLL DSQNVAPVDN
60 70 80 90 100
AWISLISKEN LLHQFQILKS RENKETLPLY GVPIAVKDNI DVRGLPTTAA
110 120 130 140 150
CPSFAYEPSK DSKVVELLRN AGAIIVGKTN LDQFATGLVG TRSPYGKTPC
160 170 180 190 200
AFSKEHVSGG SSAGSASVVA RGIVPIALGT DTAGSGRVPA ALNNLIGLKP
210 220 230 240 250
TKGVFSCQGV VPACKSLDCV SIFALNLSDA ERCFRIMCQP DPDNDEYSRP
260 270 280 290 300
YVSNPLKKFS SNVTIAIPKN IPWYGETKNP VLFSNAVENL SRTGANVIEI
310 320 330 340 350
DFEPLLELAR CLYEGTWVAE RYQAIQSFLD SKPPKESLDP TVISIIEGAK
360 370 380 390 400
KYSAVDCFSF EYKRQGILQK VRRLLESVDV LCVPTCPLNP TMQQVADEPV
410 420 430 440 450
LVNSRQGTWT NFVNLADLAA LAVPAGFRDD GLPNGITLIG KKFTDYALLE
460 470 480 490 500
LANRYFQNIF PNGSRTYGTF TSSSVKPAND QLVGPDYDPS TSIKLAVVGA
510 520 530 540 550
HLKGLPLHWQ LEKVNATYLC TTKTSKAYQL FALPKNGPVL KPGLRRVQDS
560 570 580 590 600
NGSQIELEVY SVPKELFGAF ISMVPEPLGI GSVELESGEW IKSFICEESG
610 620 630 640 650
YKAKGTVDIT KYGGFRAYFE MLKKKESQKK KLFDTVLIAN RGEIAVRIIK
660 670 680 690 700
TLKKLGIRSV AVYSDPDKYS QHVTDADVSV PLHGTTAAQT YLDMNKIIDA
710 720 730 740 750
AKQTNAQAII PGYGFLSENA DFSDACTSAG ITFVGPSGDI IRGLGLKHSA
760 770 780 790 800
RQIAQKAGVP LVPGSLLITS VEEAKKVAAE LEYPVMVKST AGGGGIGLQK
810 820 830 840 850
VDSEEDIEHI FETVKHQGET FFGDAGVFLE RFIENARHVE VQLMGDGFGK
860 870 880 890 900
AIALGERDCS LQRRNQKVIE ETPAPNLPEK TRLALRKAAE SLGSLLNYKC
910 920 930 940 950
AGTVEFIYDE KKDEFYFLEV NTRLQVEHPI TEMVTGLDLV EWMIRIAAND
960 970 980 990 1000
APDFDSTKVE VNGVSMEARL YAENPLKNFR PSPGLLVDVK FPDWARVDTW
1010 1020 1030 1040 1050
VKKGTNISPE YDPTLAKIIV HGKDRDDAIS KLNQALEETK VYGCITNIDY
1060 1070 1080 1090 1100
LKSIITSDFF AKAKVSTNIL NSYQYEPTAI EITLPGAHTS IQDYPGRVGY
1110 1120 1130 1140 1150
WRIGVPPSGP MDAYSFRLAN RIVGNDYRTP AIEVTLTGPS IVFHCETVIA
1160 1170 1180 1190 1200
ITGGTALCTL DGQEIPQHKP VEVKRGSTLS IGKLTSGCRA YLGIRGGIDV
1210 1220 1230 1240 1250
PKYLGSYSTF TLGNVGGYNG RVLKLGDVLF LPSNEENKSV ECLPQNIPQS
1260 1270 1280 1290 1300
LIPQISETKE WRIGVTCGPH GSPDFFKPES IEEFFSEKWK VHYNSNRFGV
1310 1320 1330 1340 1350
RLIGPKPKWA RSNGGEGGMH PSNTHDYVYS LGAINFTGDE PVIITCDGPS
1360 1370 1380 1390 1400
LGGFVCQAVV PEAELWKVGQ VKPGDSIQFV PLSYESSRSL KESQDVAIKS
1410 1420 1430 1440 1450
LDGTKLRRLD SVSILPSFET PILAQMEKVN ELSPKVVYRQ AGDRYVLVEY
1460 1470 1480 1490 1500
GDNEMNFNIS YRIECLISLV KKNKTIGIVE MSQGVRSVLI EFDGYKVTQK
1510 1520 1530 1540 1550
ELLKVLVAYE TEIQFDENWK ITSNIIRLPM AFEDSKTLAC VQRYQETIRS
1560 1570 1580 1590 1600
SAPWLPNNVD FIANVNGISR NEVYDMLYSA RFMVLGLGDV FLGSPCAVPL
1610 1620 1630 1640 1650
DPRHRFLGSK YNPSRTYTER GAVGIGGMYM CIYAANSPGG YQLVGRTIPI
1660 1670 1680 1690 1700
WDKLCLAASS EVPWLMNPFD QVEFYPVSEE DLDKMTEDCD NGVYKVNIEK
1710 1720 1730 1740 1750
SVFDHQEYLR WINANKDSIT AFQEGQLGER AEEFAKLIQN ANSELKESVT
1760 1770 1780 1790 1800
VKPDEEEDFP EGAEIVYSEY SGRFWKSIAS VGDVIEAGQG LLIIEAMKAE
1810 1820 1830
MIISAPKSGK IIKICHGNGD MVDSGDIVAV IETLA
Length:1,835
Mass (Da):201,832
Last modified:October 1, 1994 - v2
Checksum:iF52B0DD0FE42CD65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96P → R in AAC41643 (PubMed:1802034).Curated1
Sequence conflicti256 – 258LKK → KKN in AAC41643 (PubMed:1802034).Curated3
Sequence conflicti459I → M in AAC41643 (PubMed:1802034).Curated1
Sequence conflicti830E → K in AAC41643 (PubMed:1802034).Curated1
Sequence conflicti1395D → E in AAC41643 (PubMed:1802034).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64926 Genomic DNA Translation: AAC41643.1
Z36077 Genomic DNA Translation: CAA85172.1
Z21487 Genomic DNA Translation: CAA79695.1
BK006936 Genomic DNA Translation: DAA07325.1
PIRiS46082
RefSeqiNP_009767.1, NM_001178556.1

Genome annotation databases

EnsemblFungiiYBR208C; YBR208C; YBR208C
GeneIDi852507
KEGGisce:YBR208C

Similar proteinsi

Entry informationi

Entry nameiDUR1_YEAST
AccessioniPrimary (citable) accession number: P32528
Secondary accession number(s): D6VQK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: July 18, 2018
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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