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Entry version 163 (11 Dec 2019)
Sequence version 1 (01 Oct 1993)
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Protein

Centromere DNA-binding protein complex CBF3 subunit A

Gene

CBF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.

Miscellaneous

Present with 1350 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-30844-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Centromere DNA-binding protein complex CBF3 subunit A
Alternative name(s):
Centromere-binding factor 2
Chromosome transmission fidelity protein 14
Kinetochore protein CTF14
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CBF2
Synonyms:CBF3A, CEP2, CTF14, NDC10
Ordered Locus Names:YGR140W
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome VII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YGR140W

Saccharomyces Genome Database

More...
SGDi
S000003372 CBF2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000893691 – 956Centromere DNA-binding protein complex CBF3 subunit AAdd BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei566PhosphoserineCombined sources1
Modified residuei706PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P32504

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32504

PRoteomics IDEntifications database

More...
PRIDEi
P32504

PTM databases

CarbonylDB database of protein carbonylation sites

More...
CarbonylDBi
P32504

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P32504

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly interacts with BIR1.

Interacts with the central kinetochore protein CTF19.

2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
33386, 342 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1898 CBF3 complex

Database of interacting proteins

More...
DIPi
DIP-5955N

Protein interaction database and analysis system

More...
IntActi
P32504, 39 interactors

Molecular INTeraction database

More...
MINTi
P32504

STRING: functional protein association networks

More...
STRINGi
4932.YGR140W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P32504 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1956
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P32504

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000111344

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32504

KEGG Orthology (KO)

More...
KOi
K11574

Identification of Orthologs from Complete Genome Data

More...
OMAi
LEWFTPN

Family and domain databases

Database of protein disorder

More...
DisProti
DP02033

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.150.130, 1 hit
1.10.443.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR010998 Integrase_recombinase_N
IPR038279 Ndc10_dom2_sf
IPR031872 NDC10_II
IPR022210 TF_GCR1-like

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12550 GCR1_C, 1 hit
PF16787 NDC10_II, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32504-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT
60 70 80 90 100
KYIQWCQLNQ IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG
110 120 130 140 150
EKREETEDLD EEEENSFKIA TLKKIIGSLN FLSKLCKVHE NPNANIDTKY
160 170 180 190 200
LESVTKLHTH WIDSQKAITT NETNNTNTQV LCPPLLKVSL NLWNPETNHL
210 220 230 240 250
SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK LGKRDRDAII
260 270 280 290 300
YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV
310 320 330 340 350
SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH
360 370 380 390 400
LPYKRREYFN KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP
410 420 430 440 450
FDFKKIMNFK SPYTSYSTNA KKDPFPPPKD LLVQIFPEID EYKRHDYEGL
460 470 480 490 500
SQNSRDFLDL MEVLRERFLS NLPWIYKFFP NHDIFQDPIF GNSDFQSYFN
510 520 530 540 550
DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ LTFASSHNPD
560 570 580 590 600
THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF
610 620 630 640 650
NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI
660 670 680 690 700
KKEENIVNED GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES
710 720 730 740 750
IEDGAYGENE DENDSEMQEQ LKSMINELIN SKISTFLRDQ MDQFELKINA
760 770 780 790 800
LLDKILEEKV TRIIEQKLGS HTGKFSTLKR PQLYMTEEHN VGFDMEVPKK
810 820 830 840 850
LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE QEFMLDKSID
860 870 880 890 900
SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY
910 920 930 940 950
IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL

LVYLSN
Length:956
Mass (Da):111,917
Last modified:October 1, 1993 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC93FB7E033931E3D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti418T → A in AAU09733 (PubMed:17322287).Curated1
Sequence conflicti774K → Q in CAA79753 (PubMed:8486733).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z21627 Genomic DNA Translation: CAA79753.1
X69300 Genomic DNA Translation: CAA49158.1
Z72925 Genomic DNA Translation: CAA97153.1
AY723816 Genomic DNA Translation: AAU09733.1
BK006941 Genomic DNA Translation: DAA08231.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S64449

NCBI Reference Sequences

More...
RefSeqi
NP_011656.3, NM_001181269.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YGR140W_mRNA; YGR140W; YGR140W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
853041

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YGR140W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21627 Genomic DNA Translation: CAA79753.1
X69300 Genomic DNA Translation: CAA49158.1
Z72925 Genomic DNA Translation: CAA97153.1
AY723816 Genomic DNA Translation: AAU09733.1
BK006941 Genomic DNA Translation: DAA08231.1
PIRiS64449
RefSeqiNP_011656.3, NM_001181269.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ACOX-ray1.89A1-956[»]
6GSAelectron microscopy4.20E2-553[»]
6GYPelectron microscopy3.60E1-956[»]
6GYSelectron microscopy4.40E/L1-956[»]
6GYUelectron microscopy3.00E1-956[»]
SMRiP32504
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi33386, 342 interactors
ComplexPortaliCPX-1898 CBF3 complex
DIPiDIP-5955N
IntActiP32504, 39 interactors
MINTiP32504
STRINGi4932.YGR140W

PTM databases

CarbonylDBiP32504
iPTMnetiP32504

Proteomic databases

MaxQBiP32504
PaxDbiP32504
PRIDEiP32504

Genome annotation databases

EnsemblFungiiYGR140W_mRNA; YGR140W; YGR140W
GeneIDi853041
KEGGisce:YGR140W

Organism-specific databases

EuPathDBiFungiDB:YGR140W
SGDiS000003372 CBF2

Phylogenomic databases

HOGENOMiHOG000111344
InParanoidiP32504
KOiK11574
OMAiLEWFTPN

Enzyme and pathway databases

BioCyciYEAST:G3O-30844-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32504
RNActiP32504 protein

Family and domain databases

DisProtiDP02033
Gene3Di1.10.150.130, 1 hit
1.10.443.20, 1 hit
InterProiView protein in InterPro
IPR010998 Integrase_recombinase_N
IPR038279 Ndc10_dom2_sf
IPR031872 NDC10_II
IPR022210 TF_GCR1-like
PfamiView protein in Pfam
PF12550 GCR1_C, 1 hit
PF16787 NDC10_II, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCBF3A_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32504
Secondary accession number(s): D6VUS0, E9P954
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 11, 2019
This is version 163 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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