UniProtKB - P32502 (EI2BB_YEAST)
Translation initiation factor eIF-2B subunit beta
GCD7
Functioni
Acts as a regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site.
2 PublicationsMiscellaneous
GO - Molecular functioni
- enzyme regulator activity Source: SGD
- translation initiation factor activity Source: SGD
GO - Biological processi
- positive regulation of GTPase activity Source: ComplexPortal
- regulation of translational initiation Source: SGD
- translational initiation Source: GO_Central
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis, Translation regulation |
Enzyme and pathway databases
Reactomei | R-SCE-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Translation initiation factor eIF-2B subunit betaAlternative name(s): GCD complex subunit GCD7 Guanine nucleotide exchange factor subunit GCD7 eIF-2B GDP-GTP exchange factor subunit beta |
Gene namesi | Name:GCD7 Synonyms:TIF222 Ordered Locus Names:YLR291C ORF Names:L8003.17 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004282, GCD7 |
VEuPathDBi | FungiDB:YLR291C |
Subcellular locationi
Cytosol
- cytosol Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Other locations
- eukaryotic translation initiation factor 2B complex Source: SGD
- guanyl-nucleotide exchange factor complex Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156066 | 1 – 381 | Translation initiation factor eIF-2B subunit betaAdd BLAST | 381 |
Proteomic databases
MaxQBi | P32502 |
PaxDbi | P32502 |
PRIDEi | P32502 |
PTM databases
iPTMneti | P32502 |
Interactioni
Subunit structurei
Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.
2 PublicationsBinary interactionsi
P32502
With | #Exp. | IntAct |
---|---|---|
GCD2 [P12754] | 10 | EBI-6260,EBI-6265 |
GCN3 [P14741] | 10 | EBI-6260,EBI-6253 |
Protein-protein interaction databases
BioGRIDi | 31557, 418 interactors |
ComplexPortali | CPX-429, Eukaryotic translation initiation factor 2B complex |
DIPi | DIP-1185N |
IntActi | P32502, 156 interactors |
MINTi | P32502 |
STRINGi | 4932.YLR291C |
Miscellaneous databases
RNActi | P32502, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 148 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1465, Eukaryota |
GeneTreei | ENSGT00550000074908 |
HOGENOMi | CLU_016218_4_3_1 |
InParanoidi | P32502 |
OMAi | TSHTSYA |
Family and domain databases
Gene3Di | 1.20.120.420, 1 hit 3.40.50.10470, 1 hit |
InterProi | View protein in InterPro IPR000649, IF-2B-related IPR042529, IF_2B-like_C IPR027363, M1Pi_N IPR037171, NagB/RpiA_transferase-like |
Pfami | View protein in Pfam PF01008, IF-2B, 1 hit |
SUPFAMi | SSF100950, SSF100950, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR
60 70 80 90 100
FISAARWNHV NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE
110 120 130 140 150
EDTMSTTVTS TSVAEPLISS MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY
160 170 180 190 200
RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI HDHEILLTPT PDSKTVLKFL
210 220 230 240 250
ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET LVVPDSAVFA
260 270 280 290 300
LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK
310 320 330 340 350
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI
360 370 380
TNVGGFNPSF IYRIAWDNYK QIDVHLDKNK A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07116 Genomic DNA Translation: AAA34634.1 U17243 Genomic DNA Translation: AAB67337.1 BK006945 Genomic DNA Translation: DAA09603.1 |
PIRi | B48156 |
RefSeqi | NP_013394.1, NM_001182179.1 |
Genome annotation databases
EnsemblFungii | YLR291C_mRNA; YLR291C; YLR291C |
GeneIDi | 850998 |
KEGGi | sce:YLR291C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07116 Genomic DNA Translation: AAA34634.1 U17243 Genomic DNA Translation: AAB67337.1 BK006945 Genomic DNA Translation: DAA09603.1 |
PIRi | B48156 |
RefSeqi | NP_013394.1, NM_001182179.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6I3M | electron microscopy | 3.93 | E/F | 1-381 | [»] | |
6I7T | electron microscopy | 4.61 | E/F | 1-381 | [»] | |
6QG0 | electron microscopy | 4.20 | C/D | 1-381 | [»] | |
6QG1 | electron microscopy | 4.20 | C/D | 1-381 | [»] | |
6QG2 | electron microscopy | 4.60 | C/D | 1-381 | [»] | |
6QG3 | electron microscopy | 9.40 | C/D | 1-381 | [»] | |
6QG5 | electron microscopy | 10.10 | C/D | 1-381 | [»] | |
6QG6 | electron microscopy | 4.65 | C/D | 1-381 | [»] | |
AlphaFoldDBi | P32502 | |||||
SMRi | P32502 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31557, 418 interactors |
ComplexPortali | CPX-429, Eukaryotic translation initiation factor 2B complex |
DIPi | DIP-1185N |
IntActi | P32502, 156 interactors |
MINTi | P32502 |
STRINGi | 4932.YLR291C |
PTM databases
iPTMneti | P32502 |
Proteomic databases
MaxQBi | P32502 |
PaxDbi | P32502 |
PRIDEi | P32502 |
Genome annotation databases
EnsemblFungii | YLR291C_mRNA; YLR291C; YLR291C |
GeneIDi | 850998 |
KEGGi | sce:YLR291C |
Organism-specific databases
SGDi | S000004282, GCD7 |
VEuPathDBi | FungiDB:YLR291C |
Phylogenomic databases
eggNOGi | KOG1465, Eukaryota |
GeneTreei | ENSGT00550000074908 |
HOGENOMi | CLU_016218_4_3_1 |
InParanoidi | P32502 |
OMAi | TSHTSYA |
Enzyme and pathway databases
Reactomei | R-SCE-72731, Recycling of eIF2:GDP |
Miscellaneous databases
ChiTaRSi | GCD7, yeast |
PROi | PR:P32502 |
RNActi | P32502, protein |
Family and domain databases
Gene3Di | 1.20.120.420, 1 hit 3.40.50.10470, 1 hit |
InterProi | View protein in InterPro IPR000649, IF-2B-related IPR042529, IF_2B-like_C IPR027363, M1Pi_N IPR037171, NagB/RpiA_transferase-like |
Pfami | View protein in Pfam PF01008, IF-2B, 1 hit |
SUPFAMi | SSF100950, SSF100950, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EI2BB_YEAST | |
Accessioni | P32502Primary (citable) accession number: P32502 Secondary accession number(s): D6VYT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families