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UniProtKB - P32499 (NUP2_YEAST)

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Protein

Nucleoporin NUP2

Gene

NUP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). As one of the FG repeat nucleoporins NUP2 is involved in interactions with and guidance of nuclear transport receptors such as SRP1-KAP95 (importin alpha and beta) through the NPC. Like the closely related NUP1 it also plays an important role in disassembling and recycling SRP1-KAP95 to the cytoplasm after nuclear import. Upon entry of the heterotrimeric SRP1-KAP95-cargo complex in the nucleus, NUP2 binds through its N-terminus to the SRP1 nuclear localization signal (NLS) binding site, thus accelerating the release of the NLS-cargo. SRP1 in turn is released from NUP2 by binding of the GSP1-GTP associated export factor CSE1. NUP2 may also have a chromatin boundary/insulator activity through indirect interaction with genomic DNA via CSE1 and blocking of heterochromatin spreading.12 Publications

Miscellaneous

Present with 2960 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • importin-alpha family protein binding Source: CAFA
  • Ran GTPase binding Source: SGD
  • structural constituent of nuclear pore Source: SGD
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • G1/S transition of mitotic cell cycle Source: GO_Central
  • maintenance of chromatin silencing at telomere Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • positive regulation of GTPase activity Source: GOC
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein export from nucleus Source: SGD
  • protein localization to nuclear inner membrane Source: SGD
  • spindle organization Source: GO_Central
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • ubiquitin-dependent protein catabolic process Source: GO_Central
View the complete GO annotation on QuickGO ...

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32414-MONOMER

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP2
Alternative name(s):
Nuclear pore protein NUP2
p95
Gene namesi
Name:NUP2
Ordered Locus Names:YLR335W
ORF Names:L8300.9
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR335W
SGDiS000004327 NUP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002049031 – 720Nucleoporin NUP2Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1
Modified residuei348PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei361PhosphothreonineCombined sources1
Modified residuei581PhosphoserineCombined sources1
Modified residuei590PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32499
PaxDbiP32499
PRIDEiP32499

PTM databases

iPTMnetiP32499

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Binds to the nuclear basket of the NPC through NUP60 in a (GSP1, GSP2) GTPase-GTP-dependent manner. Interacts through its FG repeats with nuclear transport factors. Interacts with KAP122.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • importin-alpha family protein binding Source: CAFA
  • Ran GTPase binding Source: SGD
View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi31599, 200 interactors
ComplexPortaliCPX-824 Nuclear pore complex
DIPiDIP-859N
IntActiP32499, 43 interactors
MINTiP32499
STRINGi4932.YLR335W

Structurei

Secondary structure

1720
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Helixi34 – 37Combined sources4
Turni47 – 49Combined sources3

3D structure databases

DisProtiDP00222
ProteinModelPortaliP32499
SMRiP32499
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32499

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 69FXF 13
Repeati189 – 192FXFG 14
Repeati216 – 218FXF 23
Repeati247 – 249FXF 33
Repeati285 – 288FXFG 24
Repeati302 – 305FXFG 34
Repeati318 – 321FXFG 44
Repeati352 – 354FXF 43
Repeati369 – 372FXFG 54
Repeati386 – 389FXFG 64
Repeati438 – 441FXFG 74
Repeati474 – 477FXFG 84
Repeati493 – 496FXFG 94
Repeati511 – 514FXFG 104
Repeati524 – 527FXFG 114
Repeati550 – 552FXF 53
Domaini583 – 720RanBD1PROSITE-ProRule annotationAdd BLAST138

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 50Interaction with SRP1 NLS binding site 1Add BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 377Ser-richAdd BLAST45

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: FXFG repeats are especially abundant in NUPs on the nucleoplasmic side (in a highly charged environment and enriched in Ser and Thr).

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000113442
HOGENOMiHOG000248337
InParanoidiP32499
KOiK18722
OMAiDEPKPAF
OrthoDBiEOG092C4THQ

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037642 Nup2
IPR015007 NUP2/50/61
IPR011993 PH-like_dom_sf
IPR000156 Ran_bind_dom
PANTHERiPTHR23138:SF101 PTHR23138:SF101, 1 hit
PfamiView protein in Pfam
PF08911 NUP50, 1 hit
PF00638 Ran_BP1, 1 hit
SMARTiView protein in SMART
SM00160 RanBD, 1 hit
PROSITEiView protein in PROSITE
PS50196 RANBD1, 1 hit

Sequencei

Sequence statusi: Complete.

P32499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRVADAQI QRETYDSNES DDDVTPSTKV ASSAVMNRRK IAMPKRRMAF 
        60         70         80         90        100
KPFGSAKSDE TKQASSFSFL NRADGTGEAQ VDNSPTTESN SRLKALNLQF 
       110        120        130        140        150
KAKVDDLVLG KPLADLRPLF TRYELYIKNI LEAPVKSIEN PTQTKGNDAK 
       160        170        180        190        200
PAKVEDVQKS SDSSSEDEVK VEGPKFTIDA KPPISDSVFS FGPKKENRKK 
       210        220        230        240        250
DESDSENDIE IKGPEFKFSG TVSSDVFKLN PSTDKNEKKT ETNAKPFSFS 
       260        270        280        290        300
SATSTTEQTK SKNPLSLTEA TKTNVDNNSK AEASFTFGTK HAADSQNNKP 
       310        320        330        340        350
SFVFGQAAAK PSLEKSSFTF GSTTIEKKND ENSTSNSKPE KSSDSNDSNP 
       360        370        380        390        400
SFSFSIPSKN TPDASKPSFS FGVPNSSKNE TSKPVFSFGA ATPSAKEASQ 
       410        420        430        440        450
EDDNNNVEKP SSKPAFNLIS NAGTEKEKES KKDSKPAFSF GISNGSESKD 
       460        470        480        490        500
SDKPSLPSAV DGENDKKEAT KPAFSFGINT NTTKTADTKA PTFTFGSSAL 
       510        520        530        540        550
ADNKEDVKKP FSFGTSQPNN TPSFSFGKTT ANLPANSSTS PAPSIPSTGF 
       560        570        580        590        600
KFSLPFEQKG SQTTTNDSKE ESTTEATGNE SQDATKVDAT PEESKPINLQ 
       610        620        630        640        650
NGEEDEVALF SQKAKLMTFN AETKSYDSRG VGEMKLLKKK DDPSKVRLLC 
       660        670        680        690        700
RSDGMGNVLL NATVVDSFKY EPLAPGNDNL IKAPTVAADG KLVTYIVKFK 
       710        720
QKEEGRSFTK AIEDAKKEMK
Length:720
Mass (Da):77,881
Last modified:May 16, 2003 - v2
Checksum:i467210F14FA73311
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137S → F in CAA49587 (PubMed:8443417).Curated1
Sequence conflicti370S → N in CAA49587 (PubMed:8443417).Curated1
Sequence conflicti418L → F in CAA49587 (PubMed:8443417).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69964 Genomic DNA Translation: CAA49587.1
U19028 Genomic DNA Translation: AAB67259.1
BK006945 Genomic DNA Translation: DAA09641.1
PIRiS51340
RefSeqiNP_013439.1, NM_001182224.1

Genome annotation databases

EnsemblFungiiYLR335W; YLR335W; YLR335W
GeneIDi851048
KEGGisce:YLR335W

Similar proteinsi

Entry informationi

Entry nameiNUP2_YEAST
AccessioniPrimary (citable) accession number: P32499
Secondary accession number(s): D6VYX5, Q06130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 16, 2003
Last modified: July 18, 2018
This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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