UniProtKB - P32499 (NUP2_YEAST)
Nucleoporin NUP2
NUP2
Functioni
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). As one of the FG repeat nucleoporins NUP2 is involved in interactions with and guidance of nuclear transport receptors such as SRP1-KAP95 (importin alpha and beta) through the NPC. Like the closely related NUP1 it also plays an important role in disassembling and recycling SRP1-KAP95 to the cytoplasm after nuclear import. Upon entry of the heterotrimeric SRP1-KAP95-cargo complex in the nucleus, NUP2 binds through its N-terminus to the SRP1 nuclear localization signal (NLS) binding site, thus accelerating the release of the NLS-cargo. SRP1 in turn is released from NUP2 by binding of the GSP1-GTP associated export factor CSE1. NUP2 may also have a chromatin boundary/insulator activity through indirect interaction with genomic DNA via CSE1 and blocking of heterochromatin spreading.
13 PublicationsMiscellaneous
GO - Molecular functioni
- importin-alpha family protein binding Source: CAFA
- small GTPase binding Source: SGD
- structural constituent of nuclear pore Source: SGD
GO - Biological processi
- mRNA export from nucleus in response to heat stress Source: SGD
- NLS-bearing protein import into nucleus Source: SGD
- nucleocytoplasmic transport Source: ComplexPortal
- poly(A)+ mRNA export from nucleus Source: SGD
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
- protein export from nucleus Source: SGD
- protein localization to nuclear inner membrane Source: SGD
- regulation of catalytic activity Source: GOC
- silent mating-type cassette heterochromatin assembly Source: SGD
- subtelomeric heterochromatin assembly Source: SGD
- transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin NUP2Alternative name(s): Nuclear pore protein NUP2 p95 |
Gene namesi | Name:NUP2 Ordered Locus Names:YLR335W ORF Names:L8300.9 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004327, NUP2 |
VEuPathDBi | FungiDB:YLR335W |
Subcellular locationi
Nucleus
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nuclear envelope Source: ComplexPortal
- nuclear membrane Source: UniProtKB-SubCell
- nuclear pore Source: GO_Central
- nuclear pore cytoplasmic filaments Source: SGD
- nuclear pore nuclear basket Source: SGD
Other locations
- chromosome, telomeric region Source: GOC
- cytoplasm Source: CAFA
- NLS-dependent protein nuclear import complex Source: CAFA
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204903 | 1 – 720 | Nucleoporin NUP2Add BLAST | 720 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 165 | PhosphoserineCombined sources | 1 | |
Modified residuei | 203 | PhosphoserineCombined sources | 1 | |
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 348 | PhosphoserineCombined sources | 1 | |
Modified residuei | 351 | PhosphoserineCombined sources | 1 | |
Modified residuei | 361 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 581 | PhosphoserineCombined sources | 1 | |
Modified residuei | 590 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32499 |
PaxDbi | P32499 |
PRIDEi | P32499 |
PTM databases
iPTMneti | P32499 |
Interactioni
Subunit structurei
Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Binds to the nuclear basket of the NPC through NUP60 in a (GSP1, GSP2) GTPase-GTP-dependent manner.
Interacts through its FG repeats with nuclear transport factors.
Interacts with KAP122.
7 PublicationsBinary interactionsi
P32499
With | #Exp. | IntAct |
---|---|---|
KAP95 [Q06142] | 5 | EBI-12401,EBI-9145 |
NUP60 [P39705] | 2 | EBI-12401,EBI-20731 |
SRP1 [Q02821] | 4 | EBI-12401,EBI-1797 |
GO - Molecular functioni
- importin-alpha family protein binding Source: CAFA
- small GTPase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31599, 208 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-859N |
IntActi | P32499, 31 interactors |
MINTi | P32499 |
STRINGi | 4932.YLR335W |
Miscellaneous databases
RNActi | P32499, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P32499 |
SMRi | P32499 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32499 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 67 – 69 | FXF 1 | 3 | |
Repeati | 189 – 192 | FXFG 1 | 4 | |
Repeati | 216 – 218 | FXF 2 | 3 | |
Repeati | 247 – 249 | FXF 3 | 3 | |
Repeati | 285 – 288 | FXFG 2 | 4 | |
Repeati | 302 – 305 | FXFG 3 | 4 | |
Repeati | 318 – 321 | FXFG 4 | 4 | |
Repeati | 352 – 354 | FXF 4 | 3 | |
Repeati | 369 – 372 | FXFG 5 | 4 | |
Repeati | 386 – 389 | FXFG 6 | 4 | |
Repeati | 438 – 441 | FXFG 7 | 4 | |
Repeati | 474 – 477 | FXFG 8 | 4 | |
Repeati | 493 – 496 | FXFG 9 | 4 | |
Repeati | 511 – 514 | FXFG 10 | 4 | |
Repeati | 524 – 527 | FXFG 11 | 4 | |
Repeati | 550 – 552 | FXF 5 | 3 | |
Domaini | 583 – 720 | RanBD1PROSITE-ProRule annotationAdd BLAST | 138 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 33 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 35 – 50 | Interaction with SRP1 NLS binding site 1Add BLAST | 16 | |
Regioni | 52 – 92 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 136 – 278 | DisorderedSequence analysisAdd BLAST | 143 | |
Regioni | 315 – 604 | DisorderedSequence analysisAdd BLAST | 290 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 23 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 58 – 92 | Polar residuesSequence analysisAdd BLAST | 35 | |
Compositional biasi | 149 – 175 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 192 – 213 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 243 – 278 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 315 – 330 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 338 – 400 | Polar residuesSequence analysisAdd BLAST | 63 | |
Compositional biasi | 437 – 453 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 454 – 470 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 474 – 499 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 510 – 547 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 554 – 585 | Polar residuesSequence analysisAdd BLAST | 32 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0864, Eukaryota KOG4719, Eukaryota |
HOGENOMi | CLU_018357_0_0_1 |
InParanoidi | P32499 |
OMAi | SDGMGHI |
Family and domain databases
DisProti | DP00222 |
Gene3Di | 2.30.29.30, 1 hit |
IDEALi | IID50007 |
InterProi | View protein in InterPro IPR015007, NUP2/50/61 IPR011993, PH-like_dom_sf IPR000156, Ran_bind_dom IPR045255, RanBP1-like |
PANTHERi | PTHR23138, PTHR23138, 1 hit |
Pfami | View protein in Pfam PF08911, NUP50, 1 hit PF00638, Ran_BP1, 1 hit |
SMARTi | View protein in SMART SM00160, RanBD, 1 hit |
PROSITEi | View protein in PROSITE PS50196, RANBD1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAKRVADAQI QRETYDSNES DDDVTPSTKV ASSAVMNRRK IAMPKRRMAF
60 70 80 90 100
KPFGSAKSDE TKQASSFSFL NRADGTGEAQ VDNSPTTESN SRLKALNLQF
110 120 130 140 150
KAKVDDLVLG KPLADLRPLF TRYELYIKNI LEAPVKSIEN PTQTKGNDAK
160 170 180 190 200
PAKVEDVQKS SDSSSEDEVK VEGPKFTIDA KPPISDSVFS FGPKKENRKK
210 220 230 240 250
DESDSENDIE IKGPEFKFSG TVSSDVFKLN PSTDKNEKKT ETNAKPFSFS
260 270 280 290 300
SATSTTEQTK SKNPLSLTEA TKTNVDNNSK AEASFTFGTK HAADSQNNKP
310 320 330 340 350
SFVFGQAAAK PSLEKSSFTF GSTTIEKKND ENSTSNSKPE KSSDSNDSNP
360 370 380 390 400
SFSFSIPSKN TPDASKPSFS FGVPNSSKNE TSKPVFSFGA ATPSAKEASQ
410 420 430 440 450
EDDNNNVEKP SSKPAFNLIS NAGTEKEKES KKDSKPAFSF GISNGSESKD
460 470 480 490 500
SDKPSLPSAV DGENDKKEAT KPAFSFGINT NTTKTADTKA PTFTFGSSAL
510 520 530 540 550
ADNKEDVKKP FSFGTSQPNN TPSFSFGKTT ANLPANSSTS PAPSIPSTGF
560 570 580 590 600
KFSLPFEQKG SQTTTNDSKE ESTTEATGNE SQDATKVDAT PEESKPINLQ
610 620 630 640 650
NGEEDEVALF SQKAKLMTFN AETKSYDSRG VGEMKLLKKK DDPSKVRLLC
660 670 680 690 700
RSDGMGNVLL NATVVDSFKY EPLAPGNDNL IKAPTVAADG KLVTYIVKFK
710 720
QKEEGRSFTK AIEDAKKEMK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 137 | S → F in CAA49587 (PubMed:8443417).Curated | 1 | |
Sequence conflicti | 370 | S → N in CAA49587 (PubMed:8443417).Curated | 1 | |
Sequence conflicti | 418 | L → F in CAA49587 (PubMed:8443417).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69964 Genomic DNA Translation: CAA49587.1 U19028 Genomic DNA Translation: AAB67259.1 BK006945 Genomic DNA Translation: DAA09641.1 |
PIRi | S51340 |
RefSeqi | NP_013439.1, NM_001182224.1 |
Genome annotation databases
EnsemblFungii | YLR335W_mRNA; YLR335W; YLR335W |
GeneIDi | 851048 |
KEGGi | sce:YLR335W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69964 Genomic DNA Translation: CAA49587.1 U19028 Genomic DNA Translation: AAB67259.1 BK006945 Genomic DNA Translation: DAA09641.1 |
PIRi | S51340 |
RefSeqi | NP_013439.1, NM_001182224.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UN0 | X-ray | 2.60 | C/D | 1-51 | [»] | |
2C1T | X-ray | 2.60 | C/D | 1-51 | [»] | |
AlphaFoldDBi | P32499 | |||||
SMRi | P32499 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31599, 208 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-859N |
IntActi | P32499, 31 interactors |
MINTi | P32499 |
STRINGi | 4932.YLR335W |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | P32499 |
Proteomic databases
MaxQBi | P32499 |
PaxDbi | P32499 |
PRIDEi | P32499 |
Protocols and materials databases
DNASUi | 851048 |
Genome annotation databases
EnsemblFungii | YLR335W_mRNA; YLR335W; YLR335W |
GeneIDi | 851048 |
KEGGi | sce:YLR335W |
Organism-specific databases
SGDi | S000004327, NUP2 |
VEuPathDBi | FungiDB:YLR335W |
Phylogenomic databases
eggNOGi | KOG0864, Eukaryota KOG4719, Eukaryota |
HOGENOMi | CLU_018357_0_0_1 |
InParanoidi | P32499 |
OMAi | SDGMGHI |
Miscellaneous databases
EvolutionaryTracei | P32499 |
PROi | PR:P32499 |
RNActi | P32499, protein |
Family and domain databases
DisProti | DP00222 |
Gene3Di | 2.30.29.30, 1 hit |
IDEALi | IID50007 |
InterProi | View protein in InterPro IPR015007, NUP2/50/61 IPR011993, PH-like_dom_sf IPR000156, Ran_bind_dom IPR045255, RanBP1-like |
PANTHERi | PTHR23138, PTHR23138, 1 hit |
Pfami | View protein in Pfam PF08911, NUP50, 1 hit PF00638, Ran_BP1, 1 hit |
SMARTi | View protein in SMART SM00160, RanBD, 1 hit |
PROSITEi | View protein in PROSITE PS50196, RANBD1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NUP2_YEAST | |
Accessioni | P32499Primary (citable) accession number: P32499 Secondary accession number(s): D6VYX5, Q06130 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | May 16, 2003 | |
Last modified: | May 25, 2022 | |
This is version 215 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references