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Protein

H/ACA ribonucleoprotein complex subunit NHP2

Gene

NHP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP), which catalyzes pseudouridylation of rRNA and is required for ribosome biogenesis (PubMed:9843512, PubMed:9848653). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1 (PubMed:9843512, PubMed:9848653). Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs (PubMed:9843512, PubMed:9848653). The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs (PubMed:21131909). The H/ACA snoRNP complex mediates pseudouridylation at position 93 in U2 snRNA (PubMed:21131909). Essential for growth (PubMed:9843512). Directly binds H/ACA snoRNAs (PubMed:11433018).4 Publications

GO - Molecular functioni

  • box H/ACA snoRNA binding Source: UniProtKB

GO - Biological processi

  • box H/ACA snoRNP assembly Source: UniProtKB
  • cleavage involved in rRNA processing Source: SGD
  • maturation of LSU-rRNA Source: GO_Central
  • rRNA pseudouridine synthesis Source: SGD
  • snRNA pseudouridine synthesis Source: UniProtKB
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29590-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit NHP2
Alternative name(s):
H/ACA snoRNP protein NHP2
High mobility group-like nuclear protein 2
Gene namesi
Name:NHP2
Ordered Locus Names:YDL208W
ORF Names:D1045
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL208W
SGDiS000002367 NHP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56V → K: No effect. 1 Publication1
Mutagenesisi59G → E: Significant growth impairment at 30 and 37 degrees Celsius. Impaired association with H/ACA snoRNAs. 1 Publication1
Mutagenesisi68R → A: No effect. 1 Publication1
Mutagenesisi76 – 77Missing : Lethal. Impaired association with H/ACA snoRNAs. Accumulation of NHP2 within the nucleolus. 1 Publication2
Mutagenesisi80D → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367761 – 156H/ACA ribonucleoprotein complex subunit NHP2Add BLAST156

Proteomic databases

MaxQBiP32495
PaxDbiP32495
PRIDEiP32495

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10 (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909). The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909). Interacts with SHQ1 (PubMed:12228251). Interacts with NAF1 (PubMed:12515383).6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31838, 253 interactors
ComplexPortaliCPX-737 Box H/ACA ribonucleoprotein complex
DIPiDIP-5134N
IntActiP32495, 52 interactors
MINTiP32495
STRINGi4932.YDL208W

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32495
SMRiP32495
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32495

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074939
HOGENOMiHOG000055227
InParanoidiP32495
KOiK11129
OMAiNIPYCYT
OrthoDBiEOG092C5PRN

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
InterProiView protein in InterPro
IPR002415 H/ACA_rnp_Nhp2_euk
IPR029064 L30e-like
IPR004038 Ribosomal_L7Ae/L30e/S12e/Gad45
IPR018492 Ribosomal_L7Ae/L8/Nhp2
IPR004037 Ribosomal_L7Ae_CS
PfamiView protein in Pfam
PF01248 Ribosomal_L7Ae, 1 hit
PRINTSiPR00881 L7ARS6FAMILY
PR00883 NUCLEARHMG
SUPFAMiSSF55315 SSF55315, 1 hit
PROSITEiView protein in PROSITE
PS01082 RIBOSOMAL_L7AE, 1 hit

Sequencei

Sequence statusi: Complete.

P32495-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKDNKEHKE SKESKTVDNY EARMPAVLPF AKPLASKKLN KKVLKTVKKA
60 70 80 90 100
SKAKNVKRGV KEVVKALRKG EKGLVVIAGD ISPADVISHI PVLCEDHSVP
110 120 130 140 150
YIFIPSKQDL GAAGATKRPT SVVFIVPGSN KKKDGKNKEE EYKESFNEVV

KEVQAL
Length:156
Mass (Da):17,122
Last modified:February 1, 2005 - v2
Checksum:i008188132B494E5D
GO

Sequence cautioni

The sequence CAA40885 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA67483 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98786 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57714 Genomic DNA Translation: CAA40885.1 Different initiation.
X99000 Genomic DNA Translation: CAA67483.1 Different initiation.
Z74256 Genomic DNA Translation: CAA98786.1 Different initiation.
BK006938 Genomic DNA Translation: DAA11656.1
PIRiS67767
RefSeqiNP_010073.2, NM_001180268.1

Genome annotation databases

EnsemblFungiiYDL208W; YDL208W; YDL208W
GeneIDi851319
KEGGisce:YDL208W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57714 Genomic DNA Translation: CAA40885.1 Different initiation.
X99000 Genomic DNA Translation: CAA67483.1 Different initiation.
Z74256 Genomic DNA Translation: CAA98786.1 Different initiation.
BK006938 Genomic DNA Translation: DAA11656.1
PIRiS67767
RefSeqiNP_010073.2, NM_001180268.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LBWNMR-A36-156[»]
2LBXNMR-A36-156[»]
ProteinModelPortaliP32495
SMRiP32495
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31838, 253 interactors
ComplexPortaliCPX-737 Box H/ACA ribonucleoprotein complex
DIPiDIP-5134N
IntActiP32495, 52 interactors
MINTiP32495
STRINGi4932.YDL208W

Proteomic databases

MaxQBiP32495
PaxDbiP32495
PRIDEiP32495

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL208W; YDL208W; YDL208W
GeneIDi851319
KEGGisce:YDL208W

Organism-specific databases

EuPathDBiFungiDB:YDL208W
SGDiS000002367 NHP2

Phylogenomic databases

GeneTreeiENSGT00550000074939
HOGENOMiHOG000055227
InParanoidiP32495
KOiK11129
OMAiNIPYCYT
OrthoDBiEOG092C5PRN

Enzyme and pathway databases

BioCyciYEAST:G3O-29590-MONOMER

Miscellaneous databases

EvolutionaryTraceiP32495
PROiPR:P32495

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
InterProiView protein in InterPro
IPR002415 H/ACA_rnp_Nhp2_euk
IPR029064 L30e-like
IPR004038 Ribosomal_L7Ae/L30e/S12e/Gad45
IPR018492 Ribosomal_L7Ae/L8/Nhp2
IPR004037 Ribosomal_L7Ae_CS
PfamiView protein in Pfam
PF01248 Ribosomal_L7Ae, 1 hit
PRINTSiPR00881 L7ARS6FAMILY
PR00883 NUCLEARHMG
SUPFAMiSSF55315 SSF55315, 1 hit
PROSITEiView protein in PROSITE
PS01082 RIBOSOMAL_L7AE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNHP2_YEAST
AccessioniPrimary (citable) accession number: P32495
Secondary accession number(s): D6VRE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 2005
Last modified: October 10, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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