Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 170 (07 Oct 2020)
Sequence version 2 (01 Feb 2005)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

H/ACA ribonucleoprotein complex subunit NHP2

Gene

NHP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Non-catalytic component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP), which catalyzes pseudouridylation of rRNA and is required for ribosome biogenesis (PubMed:9843512, PubMed:9848653). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1 (PubMed:9843512, PubMed:9848653). Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs (PubMed:9843512, PubMed:9848653). The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs (PubMed:21131909). The H/ACA snoRNP complex mediates pseudouridylation at position 93 in U2 snRNA (PubMed:21131909). Essential for growth (PubMed:9843512). Directly binds H/ACA snoRNAs (PubMed:11433018).4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • box H/ACA snoRNA binding Source: UniProtKB
  • RNA binding Source: GO_Central

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-29590-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit NHP2
Alternative name(s):
H/ACA snoRNP protein NHP2
High mobility group-like nuclear protein 2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NHP2
Ordered Locus Names:YDL208W
ORF Names:D1045
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YDL208W

Saccharomyces Genome Database

More...
SGDi
S000002367, NHP2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi56V → K: No effect. 1 Publication1
Mutagenesisi59G → E: Significant growth impairment at 30 and 37 degrees Celsius. Impaired association with H/ACA snoRNAs. 1 Publication1
Mutagenesisi68R → A: No effect. 1 Publication1
Mutagenesisi76 – 77Missing : Lethal. Impaired association with H/ACA snoRNAs. Accumulation of NHP2 within the nucleolus. 1 Publication2
Mutagenesisi80D → A: No effect. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001367761 – 156H/ACA ribonucleoprotein complex subunit NHP2Add BLAST156

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P32495

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32495

PRoteomics IDEntifications database

More...
PRIDEi
P32495

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P32495

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs) (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909). The protein component of the H/ACA snoRNP contains CBF5, GAR1, NHP2 and NOP10 (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909). The complex contains a stable core composed of CBF5 and NOP10, to which GAR1 and NHP2 subsequently bind (PubMed:21708174, PubMed:9843512, PubMed:9848653, PubMed:21131909).

Interacts with SHQ1 (PubMed:12228251).

Interacts with NAF1 (PubMed:12515383).

6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31838, 261 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-737, Box H/ACA ribonucleoprotein complex

Database of interacting proteins

More...
DIPi
DIP-5134N

Protein interaction database and analysis system

More...
IntActi
P32495, 52 interactors

Molecular INTeraction database

More...
MINTi
P32495

STRING: functional protein association networks

More...
STRINGi
4932.YDL208W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P32495, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...
BMRBi
P32495

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P32495

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P32495

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3167, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00550000074939

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_084513_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32495

KEGG Orthology (KO)

More...
KOi
K11129

Identification of Orthologs from Complete Genome Data

More...
OMAi
IPYVWVP

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1330.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002415, H/ACA_rnp_Nhp2_euk
IPR029064, L30e-like
IPR004038, Ribosomal_L7Ae/L30e/S12e/Gad45
IPR018492, Ribosomal_L7Ae/L8/Nhp2
IPR004037, Ribosomal_L7Ae_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01248, Ribosomal_L7Ae, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00881, L7ARS6FAMILY
PR00883, NUCLEARHMG

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55315, SSF55315, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS01082, RIBOSOMAL_L7AE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32495-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKDNKEHKE SKESKTVDNY EARMPAVLPF AKPLASKKLN KKVLKTVKKA
60 70 80 90 100
SKAKNVKRGV KEVVKALRKG EKGLVVIAGD ISPADVISHI PVLCEDHSVP
110 120 130 140 150
YIFIPSKQDL GAAGATKRPT SVVFIVPGSN KKKDGKNKEE EYKESFNEVV

KEVQAL
Length:156
Mass (Da):17,122
Last modified:February 1, 2005 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i008188132B494E5D
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA40885 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA67483 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA98786 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X57714 Genomic DNA Translation: CAA40885.1 Different initiation.
X99000 Genomic DNA Translation: CAA67483.1 Different initiation.
Z74256 Genomic DNA Translation: CAA98786.1 Different initiation.
BK006938 Genomic DNA Translation: DAA11656.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S67767

NCBI Reference Sequences

More...
RefSeqi
NP_010073.2, NM_001180268.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YDL208W_mRNA; YDL208W; YDL208W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
851319

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YDL208W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57714 Genomic DNA Translation: CAA40885.1 Different initiation.
X99000 Genomic DNA Translation: CAA67483.1 Different initiation.
Z74256 Genomic DNA Translation: CAA98786.1 Different initiation.
BK006938 Genomic DNA Translation: DAA11656.1
PIRiS67767
RefSeqiNP_010073.2, NM_001180268.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LBWNMR-A36-156[»]
2LBXNMR-A36-156[»]
BMRBiP32495
SMRiP32495
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi31838, 261 interactors
ComplexPortaliCPX-737, Box H/ACA ribonucleoprotein complex
DIPiDIP-5134N
IntActiP32495, 52 interactors
MINTiP32495
STRINGi4932.YDL208W

PTM databases

iPTMnetiP32495

Proteomic databases

MaxQBiP32495
PaxDbiP32495
PRIDEiP32495

Genome annotation databases

EnsemblFungiiYDL208W_mRNA; YDL208W; YDL208W
GeneIDi851319
KEGGisce:YDL208W

Organism-specific databases

EuPathDBiFungiDB:YDL208W
SGDiS000002367, NHP2

Phylogenomic databases

eggNOGiKOG3167, Eukaryota
GeneTreeiENSGT00550000074939
HOGENOMiCLU_084513_1_1_1
InParanoidiP32495
KOiK11129
OMAiIPYVWVP

Enzyme and pathway databases

BioCyciYEAST:G3O-29590-MONOMER

Miscellaneous databases

EvolutionaryTraceiP32495

Protein Ontology

More...
PROi
PR:P32495
RNActiP32495, protein

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
InterProiView protein in InterPro
IPR002415, H/ACA_rnp_Nhp2_euk
IPR029064, L30e-like
IPR004038, Ribosomal_L7Ae/L30e/S12e/Gad45
IPR018492, Ribosomal_L7Ae/L8/Nhp2
IPR004037, Ribosomal_L7Ae_CS
PfamiView protein in Pfam
PF01248, Ribosomal_L7Ae, 1 hit
PRINTSiPR00881, L7ARS6FAMILY
PR00883, NUCLEARHMG
SUPFAMiSSF55315, SSF55315, 1 hit
PROSITEiView protein in PROSITE
PS01082, RIBOSOMAL_L7AE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNHP2_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32495
Secondary accession number(s): D6VRE6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 2005
Last modified: October 7, 2020
This is version 170 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Ribosomal proteins
    Ribosomal proteins families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again