UniProtKB - P32491 (MKK2_YEAST)
Protein
MAP kinase kinase MKK2/SSP33
Gene
MKK2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Serine/threonine protein kinase involved in a signal transduction pathway that plays a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involves the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.
Miscellaneous
Present with 1950 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 243 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 342 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 220 – 228 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase kinase activity Source: SGD
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein tyrosine kinase activity Source: UniProtKB-KW
GO - Biological processi
- activation of MAPK activity Source: GO_Central
- autophagy of peroxisome Source: SGD
- cell wall integrity MAPK cascade Source: SGD
- protein phosphorylation Source: SGD
- regulation of fungal-type cell wall organization Source: SGD
- signal transduction Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-112411, MAPK1 (ERK2) activation R-SCE-168638, NOD1/2 Signaling Pathway R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-2871796, FCERI mediated MAPK activation R-SCE-445144, Signal transduction by L1 R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-6811555, PI5P Regulates TP53 Acetylation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MKK2 Synonyms:SSP33 Ordered Locus Names:YPL140C ORF Names:LPI6C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YPL140C |
SGDi | S000006061, MKK2 |
Subcellular locationi
Cytosol
- cytosol Source: SGD
Other locations
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- cytoplasm Source: SGD
- mating projection tip Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086333 | 1 – 506 | MAP kinase kinase MKK2/SSP33Add BLAST | 506 |
Proteomic databases
MaxQBi | P32491 |
PaxDbi | P32491 |
PRIDEi | P32491 |
PTM databases
iPTMneti | P32491 |
Interactioni
Binary interactionsi
P32491
With | #Exp. | IntAct |
---|---|---|
SLT2 [Q00772] | 5 | EBI-10973,EBI-17372 |
Protein-protein interaction databases
BioGRIDi | 36042, 142 interactors |
DIPi | DIP-1447N |
IntActi | P32491, 53 interactors |
MINTi | P32491 |
STRINGi | 4932.YPL140C |
Miscellaneous databases
RNActi | P32491, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 214 – 481 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 268 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000176752 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P32491 |
OMAi | MFRPPES |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P32491-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASMFRPPES NRSHQKTPKL TLPVNLVQNA KSTNDGQHLN RSPYSSVNES
60 70 80 90 100
PYSNNSTSAT STTSSMASNS TLLYNRSSTT TIKNRPVPPP LPPLVLTQKK
110 120 130 140 150
DGIEYRVAGD SQLSERFSNL HVDITYKELL SSAPISTKLS NIDTTFIKKD
160 170 180 190 200
LDTPEGEDSY PSTLLSAYDF SSSGSNSAPL SANNIISCSN LIQGKDVDQL
210 220 230 240 250
EEEAWRFGHL KDEITTLGIL GEGAGGSVAK CRLKNGKKVF ALKTINTMNT
260 270 280 290 300
DPEYQKQIFR ELQFNKSFKS DYIVQYYGMF TDEQSSSIYI AMEYMGGKSL
310 320 330 340 350
EATYKNLLKR GGRISERVIG KIAESVLRGL SYLHERKVIH RDIKPQNILL
360 370 380 390 400
NEKGEIKLCD FGVSGEAVNS LAMTFTGTSF YMAPERIQGQ PYSVTCDVWS
410 420 430 440 450
LGLTLLEVAG GRFPFESDKI TQNVAPIELL TMILTFSPQL KDEPELDISW
460 470 480 490 500
SKTFRSFIDY CLKKDARERP SPRQMLKHPW IVGQMKKKVN MERFVKKCWE
KEKDGI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 252 | P → S in BAA02933 (PubMed:8386320).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13785 Genomic DNA Translation: BAA02933.1 U43703 Genomic DNA Translation: AAB68220.1 U10280 Genomic DNA Translation: AAB40938.1 BK006949 Genomic DNA Translation: DAA11294.1 |
PIRi | S69045 |
RefSeqi | NP_015185.1, NM_001183954.1 |
Genome annotation databases
EnsemblFungii | YPL140C_mRNA; YPL140C; YPL140C |
GeneIDi | 855963 |
KEGGi | sce:YPL140C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13785 Genomic DNA Translation: BAA02933.1 U43703 Genomic DNA Translation: AAB68220.1 U10280 Genomic DNA Translation: AAB40938.1 BK006949 Genomic DNA Translation: DAA11294.1 |
PIRi | S69045 |
RefSeqi | NP_015185.1, NM_001183954.1 |
3D structure databases
SMRi | P32491 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36042, 142 interactors |
DIPi | DIP-1447N |
IntActi | P32491, 53 interactors |
MINTi | P32491 |
STRINGi | 4932.YPL140C |
PTM databases
iPTMneti | P32491 |
Proteomic databases
MaxQBi | P32491 |
PaxDbi | P32491 |
PRIDEi | P32491 |
Genome annotation databases
EnsemblFungii | YPL140C_mRNA; YPL140C; YPL140C |
GeneIDi | 855963 |
KEGGi | sce:YPL140C |
Organism-specific databases
EuPathDBi | FungiDB:YPL140C |
SGDi | S000006061, MKK2 |
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000176752 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P32491 |
OMAi | MFRPPES |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-112411, MAPK1 (ERK2) activation R-SCE-168638, NOD1/2 Signaling Pathway R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-2871796, FCERI mediated MAPK activation R-SCE-445144, Signal transduction by L1 R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-6811555, PI5P Regulates TP53 Acetylation |
Miscellaneous databases
PROi | PR:P32491 |
RNActi | P32491, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MKK2_YEAST | |
Accessioni | P32491Primary (citable) accession number: P32491 Secondary accession number(s): D6W3M8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD