We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P32490 (MKK1_YEAST)
Protein
MAP kinase kinase MKK1/SSP32
Gene
MKK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in a signal transduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act on the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the MPK1 kinase.
Miscellaneous
Present with 1040 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 250 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 349 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 227 – 235 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase kinase activity Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
- protein tyrosine kinase activity Source: RHEA
GO - Biological processi
- autophagy of peroxisome Source: SGD
- cell wall integrity MAPK cascade Source: SGD
- MAPK cascade Source: GO_Central
- protein phosphorylation Source: SGD
- regulation of fungal-type cell wall organization Source: SGD
- signal transduction Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 984 |
Reactomei | R-SCE-168638, NOD1/2 Signaling Pathway R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-2871796, FCERI mediated MAPK activation R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-SCE-6811555, PI5P Regulates TP53 Acetylation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MKK1 Synonyms:SSP32 Ordered Locus Names:YOR231W ORF Names:O5095 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005757, MKK1 |
VEuPathDBi | FungiDB:YOR231W |
Subcellular locationi
Other locations
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- cytoplasm Source: SGD
- mating projection tip Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086332 | 1 – 508 | MAP kinase kinase MKK1/SSP32Add BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 192 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32490 |
PaxDbi | P32490 |
PRIDEi | P32490 |
PTM databases
iPTMneti | P32490 |
Interactioni
Binary interactionsi
P32490
With | #Exp. | IntAct |
---|---|---|
GIN4 [Q12263] | 2 | EBI-10968,EBI-7595 |
SLT2 [Q00772] | 4 | EBI-10968,EBI-17372 |
STE11 [P23561] | 2 | EBI-10968,EBI-18259 |
Protein-protein interaction databases
BioGRIDi | 34624, 171 interactors |
DIPi | DIP-2224N |
IntActi | P32490, 19 interactors |
MINTi | P32490 |
STRINGi | 4932.YOR231W |
Miscellaneous databases
RNActi | P32490, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 221 – 488 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 268 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 35 – 95 | DisorderedSequence analysisAdd BLAST | 61 | |
Regioni | 130 – 158 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 35 – 81 | Polar residuesSequence analysisAdd BLAST | 47 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000176752 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P32490 |
OMAi | KCWEDEA |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P32490-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASLFRPPES AKCNPNSPRL KLPLLRNNQV DENNIYLTSN GSSTTAYSSH
60 70 80 90 100
TPEPLTSSTS TLFSQTRLHP SDSSMTLNTM KKRPAPPSLP SLSINSQSKC
110 120 130 140 150
KTLPELVPIA DVSDGKHDLG LKQRVIAENE LSGNSDLTPS SMASPFSHTN
160 170 180 190 200
TSSPYLRNDL SNSVGSDFSN LISAYEQSSS PIKSSSQPKS SSESYIDLNS
210 220 230 240 250
VRDVDQLDEN GWKYANLKDR IETLGILGEG AGGSVSKCKL KNGSKIFALK
260 270 280 290 300
VINTLNTDPE YQKQIFRELQ FNRSFQSEYI VRYYGMFTDD ENSSIYIAME
310 320 330 340 350
YMGGRSLDAI YKNLLERGGR ISEKVLGKIA EAVLRGLSYL HEKKVIHRDI
360 370 380 390 400
KPQNILLNEN GQVKLCDFGV SGEAVNSLAT TFTGTSFYMA PERIQGQPYS
410 420 430 440 450
VTSDVWSLGL TILEVANGKF PCSSEKMAAN IAPFELLMWI LTFTPELKDE
460 470 480 490 500
PESNIIWSPS FKSFIDYCLK KDSRERPSPR QMINHPWIKG QMKKNVNMEK
FVRKCWKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13001 Genomic DNA Translation: BAA02364.1 Z75139 Genomic DNA Translation: CAA99451.1 AY899252 mRNA Translation: AAX83937.1 BK006948 Genomic DNA Translation: DAA11001.1 |
PIRi | A48069 |
RefSeqi | NP_014874.1, NM_001183650.1 |
Genome annotation databases
EnsemblFungii | YOR231W_mRNA; YOR231W; YOR231W |
GeneIDi | 854406 |
KEGGi | sce:YOR231W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13001 Genomic DNA Translation: BAA02364.1 Z75139 Genomic DNA Translation: CAA99451.1 AY899252 mRNA Translation: AAX83937.1 BK006948 Genomic DNA Translation: DAA11001.1 |
PIRi | A48069 |
RefSeqi | NP_014874.1, NM_001183650.1 |
3D structure databases
SMRi | P32490 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34624, 171 interactors |
DIPi | DIP-2224N |
IntActi | P32490, 19 interactors |
MINTi | P32490 |
STRINGi | 4932.YOR231W |
PTM databases
iPTMneti | P32490 |
Proteomic databases
MaxQBi | P32490 |
PaxDbi | P32490 |
PRIDEi | P32490 |
Genome annotation databases
EnsemblFungii | YOR231W_mRNA; YOR231W; YOR231W |
GeneIDi | 854406 |
KEGGi | sce:YOR231W |
Organism-specific databases
SGDi | S000005757, MKK1 |
VEuPathDBi | FungiDB:YOR231W |
Phylogenomic databases
eggNOGi | KOG0581, Eukaryota |
GeneTreei | ENSGT00940000176752 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | P32490 |
OMAi | KCWEDEA |
Enzyme and pathway databases
BRENDAi | 2.7.12.2, 984 |
Reactomei | R-SCE-168638, NOD1/2 Signaling Pathway R-SCE-2559580, Oxidative Stress Induced Senescence R-SCE-2871796, FCERI mediated MAPK activation R-SCE-450302, activated TAK1 mediates p38 MAPK activation R-SCE-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-SCE-6811555, PI5P Regulates TP53 Acetylation |
Miscellaneous databases
PROi | PR:P32490 |
RNActi | P32490, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MKK1_YEAST | |
Accessioni | P32490Primary (citable) accession number: P32490 Secondary accession number(s): D6W2T5, Q2VQW6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families