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Protein

Mitogen-activated protein kinase HOG1

Gene

HOG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription via the stress response element (STRE) in promoters of target genes. Upon osmotic shock, associates with the SKO1-SSN6-TUP1 complex, phosphorylates SKO1, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. Activates the SMP1 transcription factor and the RCK2 kinase, both also involved in the regulation of the expression of a subset of osmotic stress-related genes. Phosphorylation of HSL1 by HOG1 leads to a G2 arrest essential for cell survival at high osmolarity. Mediates also cell-cycle arrest in G1 phase by the dual targeting of SIC1. Regulates MFA2 ARE-mediated translation in response to carbon source. Targets RDP3 histone deacetylase to osmoresponsive promoters to induce gene expression on stress. Plays an essential role in maintaining water homeostasis, arsenite detoxification, copper-resistance, hydrogen peroxide response, adaptation to citric acid stress, and repression of the mating pathway activity. Required for the Golgi apparatus localization of MNN1.36 Publications

Miscellaneous

Present with 6780 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Activity regulationi

Activated by tyrosine and threonine phosphorylation. Is phosphorylated on Tyr-176 by PBS2. Inactivated by dephosphorylation by NBP2 after adaptation to osmotic stress. PTP2 and PTP3 inactivate HOG1 by dephosphorylating Tyr-176, while the PP2Cs PTC1 and PTC2 or PTC3 dephosphorylate Thr-174 in the activation loop.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 37ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: SGD
  • chromatin binding Source: SGD
  • MAP kinase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionActivator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32258-MONOMER
BRENDAi2.7.11.24 984
ReactomeiR-SCE-168638 NOD1/2 Signaling Pathway
R-SCE-171007 p38MAPK events
R-SCE-193648 NRAGE signals death through JNK
R-SCE-198753 ERK/MAPK targets
R-SCE-2559580 Oxidative Stress Induced Senescence
R-SCE-2871796 FCERI mediated MAPK activation
R-SCE-375170 CDO in myogenesis
R-SCE-418592 ADP signalling through P2Y purinoceptor 1
R-SCE-4420097 VEGFA-VEGFR2 Pathway
R-SCE-450302 activated TAK1 mediates p38 MAPK activation
R-SCE-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
R-SCE-6798695 Neutrophil degranulation
R-SCE-9007892 Interleukin-38 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1 (EC:2.7.11.24)
Short name:
MAP kinase HOG1
Alternative name(s):
High osmolarity glycerol response protein 1
Gene namesi
Name:HOG1
Synonyms:SSK3
Ordered Locus Names:YLR113W
ORF Names:L2931, L9354.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR113W
SGDiS000004103 HOG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52K → R: Impairs catalytic activity, nuclear translocation, expression of CTT1 and increases sensitivity to osmotic shock. 4 Publications1
Mutagenesisi68Y → H: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi144D → A: Impairs catalytic activity and nuclear translocation. 1 Publication1
Mutagenesisi170D → A: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi174T → A: Impairs catalytic activity, expression of CTT1 and increases sensitivity to osmotic shock. 2 Publications1
Mutagenesisi176Y → F: Impairs expression of CTT1 and increases sensitivity to osmotic shock. 2 Publications1
Mutagenesisi314A → T: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi318F → L or S: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi320W → R: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi322F → L: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi332W → R: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1
Mutagenesisi391N → D: Activates HOG1 in a constitutive manner, without the need of a stimulating stress. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001863312 – 435Mitogen-activated protein kinase HOG1Add BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei174Phosphothreonine1 Publication1
Modified residuei176PhosphotyrosineCombined sources1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme.14 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32485
PaxDbiP32485
PRIDEiP32485

PTM databases

iPTMnetiP32485

Expressioni

Inductioni

By osmotic stress, cold stress, citric acid, and in presence of bacterial lipopolysaccharides (LPS).1 Publication

Interactioni

Subunit structurei

Interacts with CDC37, HOT1, KIN28, PTP2, PTP3, RBP1, RCK2, RPD3, SIC1, SMP1 and SIN4.10 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31384, 535 interactors
DIPiDIP-1558N
ELMiP32485
IntActiP32485, 32 interactors
MINTiP32485
STRINGi4932.YLR113W

Structurei

3D structure databases

ProteinModelPortaliP32485
SMRiP32485
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 302Protein kinasePROSITE-ProRule annotationAdd BLAST280

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi174 – 176TXY3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi364 – 379Ala-richAdd BLAST16

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
InParanoidiP32485
KOiK04441
OMAiDVIDTIC
OrthoDBiEOG092C2FL8

Family and domain databases

CDDicd07856 STKc_Sty1_Hog1, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR038783 MAPK_Sty1/Hog1
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24055:SF247 PTHR24055:SF247, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32485-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTNEEFIRT QIFGTVFEIT NRYNDLNPVG MGAFGLVCSA TDTLTSQPVA
60 70 80 90 100
IKKIMKPFST AVLAKRTYRE LKLLKHLRHE NLICLQDIFL SPLEDIYFVT
110 120 130 140 150
ELQGTDLHRL LQTRPLEKQF VQYFLYQILR GLKYVHSAGV IHRDLKPSNI
160 170 180 190 200
LINENCDLKI CDFGLARIQD PQMTGYVSTR YYRAPEIMLT WQKYDVEVDI
210 220 230 240 250
WSAGCIFAEM IEGKPLFPGK DHVHQFSIIT DLLGSPPKDV INTICSENTL
260 270 280 290 300
KFVTSLPHRD PIPFSERFKT VEPDAVDLLE KMLVFDPKKR ITAADALAHP
310 320 330 340 350
YSAPYHDPTD EPVADAKFDW HFNDADLPVD TWRVMMYSEI LDFHKIGGSD
360 370 380 390 400
GQIDISATFD DQVAAATAAA AQAQAQAQAQ VQLNMAAHSH NGAGTTGNDH
410 420 430
SDIAGGNKVS DHVAANDTIT DYGNQAIQYA NEFQQ
Length:435
Mass (Da):48,858
Last modified:November 1, 1997 - v2
Checksum:iD95A20242587CE06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9R → G in AAB67558 (PubMed:9090053).Curated1
Sequence conflicti409 – 435VSDHV…NEFQQ → GQRSCSCK in AAA34680 (PubMed:7681220).CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06279 Genomic DNA Translation: AAA34680.1
U53878 Genomic DNA Translation: AAB67558.1
Z73285 Genomic DNA Translation: CAA97680.1
X89514 Genomic DNA Translation: CAA61691.1
BK006945 Genomic DNA Translation: DAA09427.1
PIRiS64950
RefSeqiNP_013214.1, NM_001182000.1

Genome annotation databases

EnsemblFungiiYLR113W; YLR113W; YLR113W
GeneIDi850803
KEGGisce:YLR113W

Similar proteinsi

Entry informationi

Entry nameiHOG1_YEAST
AccessioniPrimary (citable) accession number: P32485
Secondary accession number(s): D6VYB1, Q06232, Q12294
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 191 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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