UniProtKB - P32476 (ERG1_YEAST)
Protein
Squalene monooxygenase
Gene
ERG1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.3 Publications
Miscellaneous
Present with 65400 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- O2 + reduced [NADPH—hemoprotein reductase] + squalene = (S)-2,3-epoxysqualene + H+ + H2O + oxidized [NADPH—hemoprotein reductase]3 PublicationsEC:1.14.14.173 Publications
Cofactori
FADBy similarity
Activity regulationi
Inhibited by the allylamine antimycotic drugs.
Kineticsi
- KM=13.5 µM for squalene1 Publication
pH dependencei
Optimum pH is 7.5.1 Publication
: lanosterol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.3 PublicationsProteins known to be involved in the 3 steps of the subpathway in this organism are:
- Squalene synthase (ERG9)
- Squalene monooxygenase (ERG1)
- Lanosterol synthase (ERG7)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 56 | FADBy similarity | 1 | |
Sitei | 90 | Important for enzyme activityBy similarity | 1 | |
Binding sitei | 158 | FADBy similarity | 1 | |
Binding sitei | 335 | FADBy similarity | 1 | |
Binding sitei | 348 | FAD; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 28 – 29 | FADBy similarity | 2 | |
Nucleotide bindingi | 48 – 49 | FADBy similarity | 2 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- squalene monooxygenase activity Source: SGD
GO - Biological processi
- ergosterol biosynthetic process Source: SGD
- sterol biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | MetaCyc:YGR175C-MONOMER |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
UniPathwayi | UPA00767;UER00752 |
Names & Taxonomyi
Protein namesi | Recommended name: Squalene monooxygenase (EC:1.14.14.173 Publications)Alternative name(s): Squalene epoxidase1 Publication Short name: SE |
Gene namesi | Name:ERG1 Ordered Locus Names:YGR175C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003407, ERG1 |
VEuPathDBi | FungiDB:YGR175C |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane 1 Publication; Multi-pass membrane protein Curated
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Curated
Other locations
- Lipid droplet 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- lipid droplet Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 474 | LumenalSequence analysisAdd BLAST | 437 | |
Transmembranei | 475 – 495 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 496 | CytoplasmicSequence analysis | 1 |
Keywords - Cellular componenti
Endoplasmic reticulum, Lipid droplet, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209851 | 1 – 496 | Squalene monooxygenaseAdd BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 284 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 289 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 311 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P32476 |
PaxDbi | P32476 |
PRIDEi | P32476 |
TopDownProteomicsi | P32476 |
PTM databases
iPTMneti | P32476 |
Interactioni
Subunit structurei
Interacts with ERG28.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33428, 61 interactors |
DIPi | DIP-6325N |
IntActi | P32476, 17 interactors |
MINTi | P32476 |
STRINGi | 4932.YGR175C |
Miscellaneous databases
RNActi | P32476, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the squalene monooxygenase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1298, Eukaryota |
GeneTreei | ENSGT00390000011759 |
HOGENOMi | CLU_026390_0_0_1 |
InParanoidi | P32476 |
OMAi | LILWKAC |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
P32476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAVNVAPEL INADNTITYD AIVIGAGVIG PCVATGLARK GKKVLIVERD
60 70 80 90 100
WAMPDRIVGE LMQPGGVRAL RSLGMIQSIN NIEAYPVTGY TVFFNGEQVD
110 120 130 140 150
IPYPYKADIP KVEKLKDLVK DGNDKVLEDS TIHIKDYEDD ERERGVAFVH
160 170 180 190 200
GRFLNNLRNI TAQEPNVTRV QGNCIEILKD EKNEVVGAKV DIDGRGKVEF
210 220 230 240 250
KAHLTFICDG IFSRFRKELH PDHVPTVGSS FVGMSLFNAK NPAPMHGHVI
260 270 280 290 300
LGSDHMPILV YQISPEETRI LCAYNSPKVP ADIKSWMIKD VQPFIPKSLR
310 320 330 340 350
PSFDEAVSQG KFRAMPNSYL PARQNDVTGM CVIGDALNMR HPLTGGGMTV
360 370 380 390 400
GLHDVVLLIK KIGDLDFSDR EKVLDELLDY HFERKSYDSV INVLSVALYS
410 420 430 440 450
LFAADSDNLK ALQKGCFKYF QRGGDCVNKP VEFLSGVLPK PLQLTRVFFA
460 470 480 490
VAFYTIYLNM EERGFLGLPM ALLEGIMILI TAIRVFTPFL FGELIG
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 251 | L → F in strain: A2-M8; confers resistance to the allylamine antifungal terbinafine. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64994 Genomic DNA Translation: AAA34592.1 Z72960 Genomic DNA Translation: CAA97201.1 BK006941 Genomic DNA Translation: DAA08271.1 |
PIRi | S64489 |
RefSeqi | NP_011691.1, NM_001181304.1 |
Genome annotation databases
EnsemblFungii | YGR175C_mRNA; YGR175C; YGR175C |
GeneIDi | 853086 |
KEGGi | sce:YGR175C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64994 Genomic DNA Translation: AAA34592.1 Z72960 Genomic DNA Translation: CAA97201.1 BK006941 Genomic DNA Translation: DAA08271.1 |
PIRi | S64489 |
RefSeqi | NP_011691.1, NM_001181304.1 |
3D structure databases
SMRi | P32476 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33428, 61 interactors |
DIPi | DIP-6325N |
IntActi | P32476, 17 interactors |
MINTi | P32476 |
STRINGi | 4932.YGR175C |
Chemistry databases
ChEMBLi | CHEMBL1888 |
PTM databases
iPTMneti | P32476 |
Proteomic databases
MaxQBi | P32476 |
PaxDbi | P32476 |
PRIDEi | P32476 |
TopDownProteomicsi | P32476 |
Genome annotation databases
EnsemblFungii | YGR175C_mRNA; YGR175C; YGR175C |
GeneIDi | 853086 |
KEGGi | sce:YGR175C |
Organism-specific databases
SGDi | S000003407, ERG1 |
VEuPathDBi | FungiDB:YGR175C |
Phylogenomic databases
eggNOGi | KOG1298, Eukaryota |
GeneTreei | ENSGT00390000011759 |
HOGENOMi | CLU_026390_0_0_1 |
InParanoidi | P32476 |
OMAi | LILWKAC |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00752 |
BioCyci | MetaCyc:YGR175C-MONOMER |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
Miscellaneous databases
PROi | PR:P32476 |
RNActi | P32476, protein |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ERG1_YEAST | |
Accessioni | P32476Primary (citable) accession number: P32476 Secondary accession number(s): D6VUW0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families