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Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

PDB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.

Miscellaneous

Present with 9970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95Thiamine pyrophosphateBy similarity1

GO - Molecular functioni

  • pyruvate dehydrogenase (acetyl-transferring) activity Source: SGD

GO - Biological processi

  • acetyl-CoA biosynthetic process from pyruvate Source: SGD
  • glycolytic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandPyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YBR221C-MONOMER
ReactomeiR-SCE-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-SCE-389661 Glyoxylate metabolism and glycine degradation
R-SCE-5362517 Signaling by Retinoic Acid
R-SCE-70268 Pyruvate metabolism
R-SCE-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
Alternative name(s):
Pyruvate dehydrogenase complex component E1 beta
Short name:
PDHE1-B
Gene namesi
Name:PDB1
Ordered Locus Names:YBR221C
ORF Names:YBR1511
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR221C
SGDiS000000425 PDB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000002046134 – 366Pyruvate dehydrogenase E1 component subunit beta, mitochondrialAdd BLAST333

Proteomic databases

MaxQBiP32473
PaxDbiP32473
PRIDEiP32473

2D gel databases

SWISS-2DPAGEiP32473

PTM databases

iPTMnetiP32473

Interactioni

Subunit structurei

Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32918, 359 interactors
ComplexPortaliCPX-3207 Mitochondrial pyruvate dehydrogenase complex
DIPiDIP-1499N
IntActiP32473, 37 interactors
MINTiP32473
STRINGi4932.YBR221C

Structurei

3D structure databases

ProteinModelPortaliP32473
SMRiP32473
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063423
HOGENOMiHOG000281450
InParanoidiP32473
KOiK00162
OMAiSRMRHHC
OrthoDBiEOG092C45LK

Family and domain databases

Gene3Di3.40.50.920, 1 hit
InterProiView protein in InterPro
IPR027110 PDHB
IPR029061 THDP-binding
IPR009014 Transketo_C/PFOR_II
IPR005475 Transketolase-like_Pyr-bd
IPR033248 Transketolase_C
PANTHERiPTHR11624:SF94 PTHR11624:SF94, 1 hit
PfamiView protein in Pfam
PF02779 Transket_pyr, 1 hit
PF02780 Transketolase_C, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 1 hit
SSF52922 SSF52922, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA
60 70 80 90 100
EELDRDDDVF LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG
110 120 130 140 150
LAVGAALKGL KPIVEFMSFN FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF
160 170 180 190 200
RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV LVPYSAEDAR GLLKAAIRDP
210 220 230 240 250
NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT DISIVTYTRN
260 270 280 290 300
VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES
310 320 330 340 350
TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP
360
DTPTIVKAVK EVLSIE
Length:366
Mass (Da):40,054
Last modified:October 1, 1994 - v2
Checksum:i547FD49ED2CB8A97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160V → L in AAA34583 (PubMed:8433986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98476 Genomic DNA Translation: AAA34583.1
Z36090 Genomic DNA Translation: CAA85184.1
AY692982 Genomic DNA Translation: AAT93001.1
BK006936 Genomic DNA Translation: DAA07337.1
PIRiS46097
RefSeqiNP_009780.1, NM_001178569.1

Genome annotation databases

EnsemblFungiiYBR221C; YBR221C; YBR221C
GeneIDi852522
KEGGisce:YBR221C

Similar proteinsi

Entry informationi

Entry nameiODPB_YEAST
AccessioniPrimary (citable) accession number: P32473
Secondary accession number(s): D6VQL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: July 18, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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