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Entry version 173 (02 Jun 2021)
Sequence version 1 (01 Oct 1993)
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Protein

Diphthine methyl ester synthase

Gene

DPH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

2 Publications

Miscellaneous

2-[3-carboxy-3-(methylammonio)propyl]-L-histidine and the corresponding dimethyl compound can also act as acceptors.
Present with 13480 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei9S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei88S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei164S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei222S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei247S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • diphthine synthase activity Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-15582

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.1.1.314, 984

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-5358493, Synthesis of diphthamide-EEF2

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00559

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Diphthine methyl ester synthase (EC:2.1.1.3141 Publication)
Alternative name(s):
Diphthamide biosynthesis methyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DPH5
Ordered Locus Names:YLR172C
ORF Names:L9470.17
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000004162, DPH5

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YLR172C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001561471 – 300Diphthine methyl ester synthaseAdd BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei172PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P32469

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32469

PRoteomics IDEntifications database

More...
PRIDEi
P32469

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P32469

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31443, 208 interactors

Database of interacting proteins

More...
DIPi
DIP-4237N

Protein interaction database and analysis system

More...
IntActi
P32469, 7 interactors

Molecular INTeraction database

More...
MINTi
P32469

STRING: functional protein association networks

More...
STRINGi
4932.YLR172C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P32469, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P32469

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni113 – 114S-adenosyl-L-methionine bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the diphthine synthase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3123, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000010568

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_066040_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32469

Identification of Orthologs from Complete Genome Data

More...
OMAi
TAGDPMV

Family and domain databases

Conserved Domains Database

More...
CDDi
cd11647, DHP5_DphB, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.950.10, 1 hit
3.40.1010.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01084, Diphthine_synth, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000878, 4pyrrol_Mease
IPR035996, 4pyrrol_Methylase_sf
IPR014777, 4pyrrole_Mease_sub1
IPR014776, 4pyrrole_Mease_sub2
IPR004551, Dphthn_synthase

The PANTHER Classification System

More...
PANTHERi
PTHR10882, PTHR10882, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00590, TP_methylase, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF036432, Diphthine_synth, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53790, SSF53790, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00522, dph5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32469-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLYLIGLGLS YKSDITVRGL EAIKKCSRVY LEHYTSILMA ASQEELESYY
60 70 80 90 100
GKEIILADRE LVETGSKQIL NNADKEDVAF LVVGDPFGAT THTDLVLRAK
110 120 130 140 150
REAIPVEIIH NASVMNAVGA CGLQLYNFGQ TVSMVFFTDN WRPDSWYDKI
160 170 180 190 200
WENRKIGLHT LVLLDIKVKE QSIENMARGR LIYEPPRYMS IAQCCEQLLE
210 220 230 240 250
IEEKRGTKAY TPDTPAVAIS RLGSSSQSFK SGTISELANY DSGEPLHSLV
260 270 280 290 300
ILGRQCHELE LEYLLEFADD KEKFGKDVAN DQEYFKPAAW VPPTEDDSDE
Length:300
Mass (Da):33,847
Last modified:October 1, 1993 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i405411F6BF2E6DF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti287P → L in AAS56273 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M83375 Genomic DNA Translation: AAA34577.1
U17246 Genomic DNA Translation: AAB67469.1
AY557947 Genomic DNA Translation: AAS56273.1
X70279 Genomic DNA Translation: CAA49764.1
BK006945 Genomic DNA Translation: DAA09492.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S30890

NCBI Reference Sequences

More...
RefSeqi
NP_013273.1, NM_001182059.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLR172C_mRNA; YLR172C; YLR172C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850869

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLR172C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83375 Genomic DNA Translation: AAA34577.1
U17246 Genomic DNA Translation: AAB67469.1
AY557947 Genomic DNA Translation: AAS56273.1
X70279 Genomic DNA Translation: CAA49764.1
BK006945 Genomic DNA Translation: DAA09492.1
PIRiS30890
RefSeqiNP_013273.1, NM_001182059.1

3D structure databases

SMRiP32469
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi31443, 208 interactors
DIPiDIP-4237N
IntActiP32469, 7 interactors
MINTiP32469
STRINGi4932.YLR172C

PTM databases

iPTMnetiP32469

Proteomic databases

MaxQBiP32469
PaxDbiP32469
PRIDEiP32469

Genome annotation databases

EnsemblFungiiYLR172C_mRNA; YLR172C; YLR172C
GeneIDi850869
KEGGisce:YLR172C

Organism-specific databases

SGDiS000004162, DPH5
VEuPathDBiFungiDB:YLR172C

Phylogenomic databases

eggNOGiKOG3123, Eukaryota
GeneTreeiENSGT00390000010568
HOGENOMiCLU_066040_1_0_1
InParanoidiP32469
OMAiTAGDPMV

Enzyme and pathway databases

UniPathwayiUPA00559
BioCyciMetaCyc:MONOMER-15582
BRENDAi2.1.1.314, 984
ReactomeiR-SCE-5358493, Synthesis of diphthamide-EEF2

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32469
RNActiP32469, protein

Family and domain databases

CDDicd11647, DHP5_DphB, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01084, Diphthine_synth, 1 hit
InterProiView protein in InterPro
IPR000878, 4pyrrol_Mease
IPR035996, 4pyrrol_Methylase_sf
IPR014777, 4pyrrole_Mease_sub1
IPR014776, 4pyrrole_Mease_sub2
IPR004551, Dphthn_synthase
PANTHERiPTHR10882, PTHR10882, 1 hit
PfamiView protein in Pfam
PF00590, TP_methylase, 1 hit
PIRSFiPIRSF036432, Diphthine_synth, 1 hit
SUPFAMiSSF53790, SSF53790, 1 hit
TIGRFAMsiTIGR00522, dph5, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDPH5_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32469
Secondary accession number(s): D6VYH6, Q6Q5L4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 2, 2021
This is version 173 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families
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