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UniProtKB - P32467 (HXT4_YEAST)
Protein
Low-affinity glucose transporter HXT4
Gene
HXT4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Low-affinity glucose transporter. Can also transport xylose.
1 PublicationMiscellaneous
Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.
Activity regulationi
Xylose uptake is strongly inhibited by glucose.
Kineticsi
- KM=170 mM for xylose uptake1 Publication
- Vmax=190 nmol/min/mg enzyme for xylose uptake1 Publication
GO - Molecular functioni
- carbohydrate:proton symporter activity Source: GO_Central
- fructose transmembrane transporter activity Source: SGD
- glucose transmembrane transporter activity Source: SGD
- mannose transmembrane transporter activity Source: SGD
- pentose transmembrane transporter activity Source: SGD
GO - Biological processi
- carbohydrate transport Source: GO_Central
- hexose transmembrane transport Source: SGD
Keywordsi
Biological process | Sugar transport, Transport |
Protein family/group databases
TCDBi | 2.A.1.1.30, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Low-affinity glucose transporter HXT4Alternative name(s): Low-affinity glucose transporter LGT1 |
Gene namesi | Name:HXT4 Synonyms:LGT1, RAG1 Ordered Locus Names:YHR092C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001134, HXT4 |
VEuPathDBi | FungiDB:YHR092C |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 66 | CytoplasmicSequence analysisAdd BLAST | 66 | |
Transmembranei | 67 – 87 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 88 – 122 | ExtracellularSequence analysisAdd BLAST | 35 | |
Transmembranei | 123 – 143 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 144 – 149 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 150 – 170 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 171 – 180 | ExtracellularSequence analysis | 10 | |
Transmembranei | 181 – 201 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 202 – 207 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 208 – 228 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 229 – 242 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 243 – 263 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 264 – 346 | CytoplasmicSequence analysisAdd BLAST | 83 | |
Transmembranei | 347 – 363 | Helical; Name=7Sequence analysisAdd BLAST | 17 | |
Topological domaini | 364 – 369 | ExtracellularSequence analysis | 6 | |
Transmembranei | 370 – 387 | Helical; Name=8Sequence analysisAdd BLAST | 18 | |
Topological domaini | 388 – 394 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 395 – 415 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 416 – 437 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 438 – 458 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 459 – 475 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 476 – 496 | Helical; Name=11Sequence analysisAdd BLAST | 21 | |
Topological domaini | 497 | ExtracellularSequence analysis | 1 | |
Transmembranei | 498 – 518 | Helical; Name=12Sequence analysisAdd BLAST | 21 | |
Topological domaini | 519 – 576 | CytoplasmicSequence analysisAdd BLAST | 58 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050394 | 1 – 576 | Low-affinity glucose transporter HXT4Add BLAST | 576 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 45 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Glycosylationi | 425 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P32467 |
PaxDbi | P32467 |
PRIDEi | P32467 |
PTM databases
iPTMneti | P32467 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36527, 69 interactors |
DIPi | DIP-7908N |
IntActi | P32467, 11 interactors |
STRINGi | 4932.YHR092C |
Miscellaneous databases
RNActi | P32467, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 56 | DisorderedSequence analysisAdd BLAST | 56 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 37 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 38 – 56 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0254, Eukaryota |
GeneTreei | ENSGT00940000176280 |
HOGENOMi | CLU_001265_30_1_1 |
InParanoidi | P32467 |
OMAi | HIGWTSP |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32467-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEEAAYQED TAVQNTPADA LSPVESDSNS ALSTPSNKAE RDDMKDFDEN
60 70 80 90 100
HEESNNYVEI PKKPASAYVT VSICCLMVAF GGFVFGWDTG TISGFVAQTD
110 120 130 140 150
FIRRFGMKHH DGTYYLSKVR TGLIVSIFNI GCAIGGIILA KLGDMYGRKM
160 170 180 190 200
GLIVVVVIYI IGIIIQIASI NKWYQYFIGR IISGLGVGGI AVLSPMLISE
210 220 230 240 250
VSPKHIRGTL VSCYQLMITL GIFLGYCTNY GTKTYTNSVQ WRVPLGLGFA
260 270 280 290 300
WALFMIGGMT FVPESPRYLV EVGKIEEAKR SIALSNKVSA DDPAVMAEVE
310 320 330 340 350
VVQATVEAEK LAGNASWGEI FSTKTKVFQR LIMGAMIQSL QQLTGDNYFF
360 370 380 390 400
YYGTTVFTAV GLEDSFETSI VLGIVNFAST FVGIFLVERY GRRRCLLWGA
410 420 430 440 450
ASMTACMVVF ASVGVTRLWP NGKKNGSSKG AGNCMIVFTC FYLFCFATTW
460 470 480 490 500
APIPFVVNSE TFPLRVKSKC MAIAQACNWI WGFLIGFFTP FISGAIDFYY
510 520 530 540 550
GYVFMGCLVF SYFYVFFFVP ETKGLTLEEV NTLWEEGVLP WKSPSWVPPN
560 570
KRGTDYNADD LMHDDQPFYK KMFGKK
Sequence cautioni
The sequence AAB68932 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81960 Genomic DNA Translation: AAA20997.1 X67321 Genomic DNA Translation: CAA47735.1 U00060 Genomic DNA Translation: AAB68932.1 Frameshift. BK006934 Genomic DNA Translation: DAA06788.2 |
PIRi | S46724 |
RefSeqi | NP_011960.2, NM_001179222.2 |
Genome annotation databases
EnsemblFungii | YHR092C_mRNA; YHR092C; YHR092C |
GeneIDi | 856492 |
KEGGi | sce:YHR092C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81960 Genomic DNA Translation: AAA20997.1 X67321 Genomic DNA Translation: CAA47735.1 U00060 Genomic DNA Translation: AAB68932.1 Frameshift. BK006934 Genomic DNA Translation: DAA06788.2 |
PIRi | S46724 |
RefSeqi | NP_011960.2, NM_001179222.2 |
3D structure databases
AlphaFoldDBi | P32467 |
SMRi | P32467 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36527, 69 interactors |
DIPi | DIP-7908N |
IntActi | P32467, 11 interactors |
STRINGi | 4932.YHR092C |
Protein family/group databases
TCDBi | 2.A.1.1.30, the major facilitator superfamily (mfs) |
PTM databases
iPTMneti | P32467 |
Proteomic databases
MaxQBi | P32467 |
PaxDbi | P32467 |
PRIDEi | P32467 |
Genome annotation databases
EnsemblFungii | YHR092C_mRNA; YHR092C; YHR092C |
GeneIDi | 856492 |
KEGGi | sce:YHR092C |
Organism-specific databases
SGDi | S000001134, HXT4 |
VEuPathDBi | FungiDB:YHR092C |
Phylogenomic databases
eggNOGi | KOG0254, Eukaryota |
GeneTreei | ENSGT00940000176280 |
HOGENOMi | CLU_001265_30_1_1 |
InParanoidi | P32467 |
OMAi | HIGWTSP |
Miscellaneous databases
PROi | PR:P32467 |
RNActi | P32467, protein |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HXT4_YEAST | |
Accessioni | P32467Primary (citable) accession number: P32467 Secondary accession number(s): D3DL44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names - SIMILARITY comments
Index of protein domains and families