We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P32465 (HXT1_YEAST)
Protein
Low-affinity glucose transporter HXT1
Gene
HXT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Low-affinity glucose transporter. HXT1 is as well involved in the transport of mannose.
Miscellaneous
Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.
Present with 23300 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- carbohydrate:proton symporter activity Source: GO_Central
- fructose transmembrane transporter activity Source: SGD
- galactose transmembrane transporter activity Source: SGD
- glucose transmembrane transporter activity Source: SGD
- mannose transmembrane transporter activity Source: SGD
- pentose transmembrane transporter activity Source: SGD
GO - Biological processi
- carbohydrate transport Source: GO_Central
- glucose transmembrane transport Source: SGD
- mannose transmembrane transport Source: SGD
Keywordsi
Biological process | Sugar transport, Transport |
Protein family/group databases
TCDBi | 2.A.1.1.108, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Low-affinity glucose transporter HXT1 |
Gene namesi | Name:HXT1 Synonyms:HOR4 Ordered Locus Names:YHR094C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001136, HXT1 |
VEuPathDBi | FungiDB:YHR094C |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 60 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 61 – 81 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 82 – 116 | ExtracellularSequence analysisAdd BLAST | 35 | |
Transmembranei | 117 – 137 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 138 – 143 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 144 – 164 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 165 – 174 | ExtracellularSequence analysis | 10 | |
Transmembranei | 175 – 195 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 196 – 201 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 202 – 222 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 223 – 236 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 237 – 257 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 258 – 340 | CytoplasmicSequence analysisAdd BLAST | 83 | |
Transmembranei | 341 – 357 | Helical; Name=7Sequence analysisAdd BLAST | 17 | |
Topological domaini | 358 – 363 | ExtracellularSequence analysis | 6 | |
Transmembranei | 364 – 381 | Helical; Name=8Sequence analysisAdd BLAST | 18 | |
Topological domaini | 382 – 388 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 389 – 409 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 410 – 431 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 432 – 452 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 453 – 469 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 470 – 490 | Helical; Name=11Sequence analysisAdd BLAST | 21 | |
Topological domaini | 491 | ExtracellularSequence analysis | 1 | |
Transmembranei | 492 – 512 | Helical; Name=12Sequence analysisAdd BLAST | 21 | |
Topological domaini | 513 – 570 | CytoplasmicSequence analysisAdd BLAST | 58 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050391 | 1 – 570 | Low-affinity glucose transporter HXT1Add BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 38 | PhosphoserineCombined sources | 1 | |
Modified residuei | 44 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 228 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P32465 |
PaxDbi | P32465 |
PRIDEi | P32465 |
PTM databases
iPTMneti | P32465 |
Expressioni
Developmental stagei
Expression is maximal during lag and early exponential phases of growth, decreasing upon further entry into exponential growth.
Inductioni
Repressed at high glucose concentrations.
Interactioni
Protein-protein interaction databases
BioGRIDi | 36528, 128 interactors |
DIPi | DIP-5593N |
IntActi | P32465, 38 interactors |
MINTi | P32465 |
STRINGi | 4932.YHR094C |
Miscellaneous databases
RNActi | P32465, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 51 | DisorderedSequence analysisAdd BLAST | 51 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0254, Eukaryota |
GeneTreei | ENSGT00940000176280 |
HOGENOMi | CLU_001265_30_1_1 |
InParanoidi | P32465 |
OMAi | SFFTRFI |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32465-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSTPDLISP QKSNSSNSYE LESGRSKAMN TPEGKNESFH DNLSESQVQP
60 70 80 90 100
AVAPPNTGKG VYVTVSICCV MVAFGGFIFG WDTGTISGFV AQTDFLRRFG
110 120 130 140 150
MKHHDGSHYL SKVRTGLIVS IFNIGCAIGG IVLAKLGDMY GRRIGLIVVV
160 170 180 190 200
VIYTIGIIIQ IASINKWYQY FIGRIISGLG VGGITVLSPM LISEVAPSEM
210 220 230 240 250
RGTLVSCYQV MITLGIFLGY CTNFGTKNYS NSVQWRVPLG LCFAWALFMI
260 270 280 290 300
GGMMFVPESP RYLVEAGRID EARASLAKVN KCPPDHPYIQ YELETIEASV
310 320 330 340 350
EEMRAAGTAS WGELFTGKPA MFQRTMMGIM IQSLQQLTGD NYFFYYGTIV
360 370 380 390 400
FQAVGLSDSF ETSIVFGVVN FFSTCCSLYT VDRFGRRNCL MWGAVGMVCC
410 420 430 440 450
YVVYASVGVT RLWPNGQDQP SSKGAGNCMI VFACFYIFCF ATTWAPIAYV
460 470 480 490 500
VISECFPLRV KSKCMSIASA ANWIWGFLIS FFTPFITGAI NFYYGYVFMG
510 520 530 540 550
CMVFAYFYVF FFVPETKGLS LEEVNDMYAE GVLPWKSASW VPVSKRGADY
560 570
NADDLMHDDQ PFYKSLFSRK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | Missing in AAA34700 (PubMed:2046678).Curated | 1 | |
Sequence conflicti | 469 | S → T in AAA34700 (PubMed:2046678).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07079 Genomic DNA Translation: AAB59311.1 M82963 Genomic DNA Translation: AAA34700.1 U00060 Genomic DNA Translation: AAB68933.1 BK006934 Genomic DNA Translation: DAA06789.1 |
PIRi | S38798 |
RefSeqi | NP_011962.1, NM_001179224.1 |
Genome annotation databases
EnsemblFungii | YHR094C_mRNA; YHR094C; YHR094C |
GeneIDi | 856494 |
KEGGi | sce:YHR094C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07079 Genomic DNA Translation: AAB59311.1 M82963 Genomic DNA Translation: AAA34700.1 U00060 Genomic DNA Translation: AAB68933.1 BK006934 Genomic DNA Translation: DAA06789.1 |
PIRi | S38798 |
RefSeqi | NP_011962.1, NM_001179224.1 |
3D structure databases
SMRi | P32465 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36528, 128 interactors |
DIPi | DIP-5593N |
IntActi | P32465, 38 interactors |
MINTi | P32465 |
STRINGi | 4932.YHR094C |
Protein family/group databases
TCDBi | 2.A.1.1.108, the major facilitator superfamily (mfs) |
PTM databases
iPTMneti | P32465 |
Proteomic databases
MaxQBi | P32465 |
PaxDbi | P32465 |
PRIDEi | P32465 |
Genome annotation databases
EnsemblFungii | YHR094C_mRNA; YHR094C; YHR094C |
GeneIDi | 856494 |
KEGGi | sce:YHR094C |
Organism-specific databases
SGDi | S000001136, HXT1 |
VEuPathDBi | FungiDB:YHR094C |
Phylogenomic databases
eggNOGi | KOG0254, Eukaryota |
GeneTreei | ENSGT00940000176280 |
HOGENOMi | CLU_001265_30_1_1 |
InParanoidi | P32465 |
OMAi | SFFTRFI |
Miscellaneous databases
PROi | PR:P32465 |
RNActi | P32465, protein |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HXT1_YEAST | |
Accessioni | P32465Primary (citable) accession number: P32465 Secondary accession number(s): D3DL45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names - SIMILARITY comments
Index of protein domains and families