UniProtKB - P32461 (DPH2_YEAST)
Protein
2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
Gene
DPH2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.1 Publication
Miscellaneous
Present with 3486 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + H+ + S-methyl-5'-thioadenosine2 PublicationsEC:2.5.1.1082 Publications
: peptidyl-diphthamide biosynthesis Pathwayi
This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.
GO - Molecular functioni
- transferase activity Source: UniProtKB-KW
GO - Biological processi
Keywordsi
Molecular function | Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-15579 |
BRENDAi | 2.5.1.108, 984 |
Reactomei | R-SCE-5358493, Synthesis of diphthamide-EEF2 |
UniPathwayi | UPA00559 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2Curated (EC:2.5.1.1082 Publications)Alternative name(s): Diphthamide biosynthesis protein 2 Diphtheria toxin resistance protein 2 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2 |
Gene namesi | Name:DPH2 Ordered Locus Names:YKL191W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001674, DPH2 |
VEuPathDBi | FungiDB:YKL191W |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083394 | 1 – 534 | 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2Add BLAST | 534 |
Proteomic databases
MaxQBi | P32461 |
PaxDbi | P32461 |
PRIDEi | P32461 |
PTM databases
iPTMneti | P32461 |
Interactioni
Subunit structurei
Interacts with the diphthamide biosynthesis proteins DPH1 and KTI11/DPH3.
2 PublicationsBinary interactionsi
P32461
With | #Exp. | IntAct |
---|---|---|
DPH1 [P40487] | 3 | EBI-6090,EBI-25162 |
Protein-protein interaction databases
BioGRIDi | 33930, 123 interactors |
DIPi | DIP-5654N |
IntActi | P32461, 2 interactors |
MINTi | P32461 |
STRINGi | 4932.YKL191W |
Miscellaneous databases
RNActi | P32461, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2648, Eukaryota |
GeneTreei | ENSGT00940000153694 |
HOGENOMi | CLU_015210_1_0_1 |
InParanoidi | P32461 |
OMAi | FGNACRS |
Family and domain databases
Gene3Di | 3.40.50.11840, 1 hit 3.40.50.11850, 1 hit 3.40.50.11860, 1 hit |
InterProi | View protein in InterPro IPR010014, DHP2 IPR016435, DPH1/DPH2 IPR042263, DPH1/DPH2_1 IPR042264, DPH1/DPH2_2 IPR042265, DPH1/DPH2_3 |
PANTHERi | PTHR10762, PTHR10762, 1 hit PTHR10762:SF2, PTHR10762:SF2, 1 hit |
Pfami | View protein in Pfam PF01866, Diphthamide_syn, 1 hit |
SFLDi | SFLDS00032, Radical_SAM_3-amino-3-carboxyp, 1 hit |
TIGRFAMsi | TIGR00322, diphth2_R, 1 hit TIGR00272, DPH2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32461-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEVAPALSTT QSDVAFQKVE THEIDRSSYL GPCYNSDELM QLISAYYNVE
60 70 80 90 100
PLVGYLEQHP EYQNVTLQFP DDLIKDSSLI VRLLQSKFPH GKIKFWVLAD
110 120 130 140 150
TAYSACCVDE VAAEHVHAEV VVHFGDACLN AIQNLPVVYS FGTPFLDLAL
160 170 180 190 200
VVENFQRAFP DLSSKICLMA NAPFSKHLSQ LYNILKGDLH YTNIIYSQVN
210 220 230 240 250
TSAVEEKFVT ILDTFHVPED VDQVGVFEKN SVLFGQHDKA DNISPEDYHL
260 270 280 290 300
FHLTTPQDPR LLYLSTVFQS VHIFDPALPG MVTGPFPSLM RRYKYMHVAR
310 320 330 340 350
TAGCIGILVN TLSLRNTRET INELVKLIKT REKKHYLFVV GKPNVAKLAN
360 370 380 390 400
FEDIDIWCIL GCSQSGIIVD QFNEFYKPII TPYELNLALS EEVTWTGKWV
410 420 430 440 450
VDFRDAIDEI EQNLGGQDTI SASTTSDEPE FDVVRGRYTS TSRPLRALTH
460 470 480 490 500
LELEAADDDD SKQLTTRHTA SGAVIKGTVS TSASALQNRS WKGLGSDFDS
510 520 530
TEVDNTGADI EEGISGVARG YGFDREDAMK KENK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01424 Genomic DNA Translation: AAA64990.1 X69765 Genomic DNA Translation: CAA49420.1 X74151 Genomic DNA Translation: CAA52247.1 Z28191 Genomic DNA Translation: CAA82035.1 Z26521 Genomic DNA Translation: CAA81289.1 BK006944 Genomic DNA Translation: DAA08975.1 |
PIRi | B59228, S34679 |
RefSeqi | NP_012730.1, NM_001179757.1 |
Genome annotation databases
EnsemblFungii | YKL191W_mRNA; YKL191W; YKL191W |
GeneIDi | 853643 |
KEGGi | sce:YKL191W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01424 Genomic DNA Translation: AAA64990.1 X69765 Genomic DNA Translation: CAA49420.1 X74151 Genomic DNA Translation: CAA52247.1 Z28191 Genomic DNA Translation: CAA82035.1 Z26521 Genomic DNA Translation: CAA81289.1 BK006944 Genomic DNA Translation: DAA08975.1 |
PIRi | B59228, S34679 |
RefSeqi | NP_012730.1, NM_001179757.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33930, 123 interactors |
DIPi | DIP-5654N |
IntActi | P32461, 2 interactors |
MINTi | P32461 |
STRINGi | 4932.YKL191W |
PTM databases
iPTMneti | P32461 |
Proteomic databases
MaxQBi | P32461 |
PaxDbi | P32461 |
PRIDEi | P32461 |
Genome annotation databases
EnsemblFungii | YKL191W_mRNA; YKL191W; YKL191W |
GeneIDi | 853643 |
KEGGi | sce:YKL191W |
Organism-specific databases
SGDi | S000001674, DPH2 |
VEuPathDBi | FungiDB:YKL191W |
Phylogenomic databases
eggNOGi | KOG2648, Eukaryota |
GeneTreei | ENSGT00940000153694 |
HOGENOMi | CLU_015210_1_0_1 |
InParanoidi | P32461 |
OMAi | FGNACRS |
Enzyme and pathway databases
UniPathwayi | UPA00559 |
BioCyci | MetaCyc:MONOMER-15579 |
BRENDAi | 2.5.1.108, 984 |
Reactomei | R-SCE-5358493, Synthesis of diphthamide-EEF2 |
Miscellaneous databases
PROi | PR:P32461 |
RNActi | P32461, protein |
Family and domain databases
Gene3Di | 3.40.50.11840, 1 hit 3.40.50.11850, 1 hit 3.40.50.11860, 1 hit |
InterProi | View protein in InterPro IPR010014, DHP2 IPR016435, DPH1/DPH2 IPR042263, DPH1/DPH2_1 IPR042264, DPH1/DPH2_2 IPR042265, DPH1/DPH2_3 |
PANTHERi | PTHR10762, PTHR10762, 1 hit PTHR10762:SF2, PTHR10762:SF2, 1 hit |
Pfami | View protein in Pfam PF01866, Diphthamide_syn, 1 hit |
SFLDi | SFLDS00032, Radical_SAM_3-amino-3-carboxyp, 1 hit |
TIGRFAMsi | TIGR00322, diphth2_R, 1 hit TIGR00272, DPH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPH2_YEAST | |
Accessioni | P32461Primary (citable) accession number: P32461 Secondary accession number(s): D6VX09 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families