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Protein

Guanylate-binding protein 2

Gene

GBP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (PubMed:8706832). Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes (By similarity).By similarity1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.1 Publication

Kineticsi

  1. KM=313 µM for GTP1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi45 – 52GTPBy similarity8
    Nucleotide bindingi67 – 69GTPBy similarity3
    Nucleotide bindingi97 – 101GTPBy similarity5

    GO - Molecular functioni

    • GTPase activity Source: InterPro
    • GTP binding Source: ProtInc
    • protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    • cellular response to interferon-gamma Source: UniProtKB
    • cellular response to interleukin-1 Source: UniProtKB
    • cellular response to tumor necrosis factor Source: UniProtKB
    • immune response Source: ProtInc
    • interferon-gamma-mediated signaling pathway Source: Reactome
    • protein localization to nucleus Source: UniProtKB
    • type I interferon signaling pathway Source: Reactome

    Keywordsi

    Molecular functionHydrolase
    LigandGTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-HSA-877300 Interferon gamma signaling
    R-HSA-909733 Interferon alpha/beta signaling

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanylate-binding protein 2 (EC:3.6.5.-1 Publication)
    Alternative name(s):
    GTP-binding protein 2
    Short name:
    GBP-2
    Short name:
    HuGBP-2
    Guanine nucleotide-binding protein 2
    Interferon-induced guanylate-binding protein 2
    Gene namesi
    Name:GBP2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000162645.12
    HGNCiHGNC:4183 GBP2
    MIMi600412 gene
    neXtProtiNX_P32456

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi588 – 591Missing : No effect on subcellular location by confocal microscopy, but loss of membrane-association by subcellular fractionation. 1 Publication4

    Organism-specific databases

    DisGeNETi2634
    OpenTargetsiENSG00000162645
    PharmGKBiPA28597

    Polymorphism and mutation databases

    BioMutaiGBP2
    DMDMi226694187

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001909641 – 588Guanylate-binding protein 2Add BLAST588
    PropeptideiPRO_0000370782589 – 591Removed in mature formBy similarity3

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei588Cysteine methyl esterBy similarity1
    Lipidationi588S-geranylgeranyl cysteine1 Publication1

    Post-translational modificationi

    Isoprenylation is required for proper subcellular location.1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    EPDiP32456
    MaxQBiP32456
    PaxDbiP32456
    PeptideAtlasiP32456
    PRIDEiP32456
    ProteomicsDBi54878

    PTM databases

    iPTMnetiP32456
    PhosphoSitePlusiP32456

    Expressioni

    Inductioni

    By IFNG/IFN-gamma during macrophage activation, and by TNF and IL1B.1 Publication

    Gene expression databases

    BgeeiENSG00000162645
    CleanExiHS_GBP2
    GenevisibleiP32456 HS

    Organism-specific databases

    HPAiCAB045975
    HPA042682

    Interactioni

    Subunit structurei

    Homodimer; homodimerization occurs upon GTP-binding and is required for the association with membranous structures. Heterodimer with other family members, including GBP1, GBP3, GBP4 and GBP5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q9WMX22EBI-714388,EBI-6863741From Hepatitis C virus genotype 1b (isolate Con1).

    GO - Molecular functioni

    • protein homodimerization activity Source: UniProtKB

    Protein-protein interaction databases

    BioGridi108904, 45 interactors
    IntActiP32456, 24 interactors
    MINTiP32456
    STRINGi9606.ENSP00000359497

    Structurei

    3D structure databases

    ProteinModelPortaliP32456
    SMRiP32456
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini35 – 276GB1/RHD3-type GAdd BLAST242

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 309GTPase domain (Globular)By similarityAdd BLAST309

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG2037 Eukaryota
    ENOG410XR6Z LUCA
    GeneTreeiENSGT00550000074475
    HOGENOMiHOG000266974
    HOVERGENiHBG001979
    InParanoidiP32456
    KOiK20897
    OMAiFINDETY
    OrthoDBiEOG091G03P3
    PhylomeDBiP32456
    TreeFamiTF331602

    Family and domain databases

    CDDicd16269 GBP_C, 1 hit
    InterProiView protein in InterPro
    IPR030386 G_GB1_RHD3_dom
    IPR037684 GBP_C
    IPR003191 Guanylate-bd/ATL_C
    IPR036543 Guanylate-bd_C_sf
    IPR015894 Guanylate-bd_N
    IPR027417 P-loop_NTPase
    PfamiView protein in Pfam
    PF02263 GBP, 1 hit
    PF02841 GBP_C, 1 hit
    SUPFAMiSSF48340 SSF48340, 1 hit
    SSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS51715 G_GB1_RHD3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32456-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAPEINLPGP MSLIDNTKGQ LVVNPEALKI LSAITQPVVV VAIVGLYRTG
    60 70 80 90 100
    KSYLMNKLAG KKNGFSLGST VKSHTKGIWM WCVPHPKKPE HTLVLLDTEG
    110 120 130 140 150
    LGDIEKGDNE NDSWIFALAI LLSSTFVYNS MGTINQQAMD QLHYVTELTD
    160 170 180 190 200
    RIKANSSPGN NSVDDSADFV SFFPAFVWTL RDFTLELEVD GEPITADDYL
    210 220 230 240 250
    ELSLKLRKGT DKKSKSFNDP RLCIRKFFPK RKCFVFDWPA PKKYLAHLEQ
    260 270 280 290 300
    LKEEELNPDF IEQVAEFCSY ILSHSNVKTL SGGIPVNGPR LESLVLTYVN
    310 320 330 340 350
    AISSGDLPCM ENAVLALAQI ENSAAVEKAI AHYEQQMGQK VQLPTETLQE
    360 370 380 390 400
    LLDLHRDSER EAIEVFMKNS FKDVDQMFQR KLGAQLEARR DDFCKQNSKA
    410 420 430 440 450
    SSDCCMALLQ DIFGPLEEDV KQGTFSKPGG YRLFTQKLQE LKNKYYQVPR
    460 470 480 490 500
    KGIQAKEVLK KYLESKEDVA DALLQTDQSL SEKEKAIEVE RIKAESAEAA
    510 520 530 540 550
    KKMLEEIQKK NEEMMEQKEK SYQEHVKQLT EKMERDRAQL MAEQEKTLAL
    560 570 580 590
    KLQEQERLLK EGFENESKRL QKDIWDIQMR SKSLEPICNI L
    Length:591
    Mass (Da):67,209
    Last modified:April 14, 2009 - v3
    Checksum:iB09B3C2F3C3E1EA2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti310M → R in AAH73163 (PubMed:15489334).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_054815281S → P. Corresponds to variant dbSNP:rs2230336Ensembl.1
    Natural variantiVAR_054816285P → A5 PublicationsCorresponds to variant dbSNP:rs1803632Ensembl.1
    Natural variantiVAR_054817303S → G4 PublicationsCorresponds to variant dbSNP:rs2230338Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M55543 mRNA Translation: AAA67323.1
    AL832451 mRNA Translation: CAD89925.1
    AK314325 mRNA Translation: BAG36973.1
    CR457062 mRNA Translation: CAG33343.1
    AC104459 Genomic DNA No translation available.
    CH471097 Genomic DNA Translation: EAW73146.1
    BC073163 mRNA Translation: AAH73163.1
    CCDSiCCDS719.1
    PIRiS70524
    RefSeqiNP_004111.2, NM_004120.4
    UniGeneiHs.386567
    Hs.732899
    Hs.734162

    Genome annotation databases

    EnsembliENST00000370466; ENSP00000359497; ENSG00000162645
    ENST00000464839; ENSP00000434282; ENSG00000162645
    GeneIDi2634
    KEGGihsa:2634
    UCSCiuc001dmz.3 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiGBP2_HUMAN
    AccessioniPrimary (citable) accession number: P32456
    Secondary accession number(s): Q6GPH0, Q6IAU2, Q86TB0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 14, 2009
    Last modified: June 20, 2018
    This is version 161 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

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