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Protein

Guanylate-binding protein 1

Gene

GBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52GTP8
Nucleotide bindingi67 – 69GTP3
Nucleotide bindingi97 – 101GTP5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAntiviral defense, Immunity
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate-binding protein 1 (EC:3.6.5.-2 Publications)
Alternative name(s):
GTP-binding protein 1
Short name:
GBP-1
Short name:
HuGBP-1
Guanine nucleotide-binding protein 1
Interferon-induced guanylate-binding protein 1
Gene namesi
Name:GBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000117228.9
HGNCiHGNC:4182 GBP1
MIMi600411 gene
neXtProtiNX_P32455

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51K → A: Loss of GTPase activity. Constitutively monomeric. Expressed throughout the cytoplasm, loss of vesicular accumulation. 1 Publication1
Mutagenesisi227 – 228RK → EE: Constitutively dimeric. Localizes at vesicle-like structures at the plasma membrane. 1 Publication2
Mutagenesisi589 – 592Missing : Loss of association with membranes. 1 Publication4

Organism-specific databases

DisGeNETi2633
OpenTargetsiENSG00000117228
PharmGKBiPA28596

Polymorphism and mutation databases

BioMutaiGBP1
DMDMi311033383

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001909631 – 589Guanylate-binding protein 1Add BLAST589
PropeptideiPRO_0000396777590 – 592Removed in mature form3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei589Cysteine methyl esterBy similarity1
Lipidationi589S-farnesyl cysteine1 Publication1

Post-translational modificationi

Isoprenylation is required for proper subcellular location.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP32455
MaxQBiP32455
PaxDbiP32455
PeptideAtlasiP32455
PRIDEiP32455
ProteomicsDBi54877

PTM databases

iPTMnetiP32455
PhosphoSitePlusiP32455
SwissPalmiP32455

Expressioni

Inductioni

By IFNG during macrophage activation, and by TNF and IL1B.1 Publication

Gene expression databases

BgeeiENSG00000117228 Expressed in 222 organ(s), highest expression level in upper lobe of lung
CleanExiHS_GBP1
GenevisibleiP32455 HS

Organism-specific databases

HPAiCAB015450

Interactioni

Subunit structurei

Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself9EBI-2869161,EBI-2869161

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108903, 20 interactors
DIPiDIP-60423N
IntActiP32455, 2 interactors
MINTiP32455
STRINGi9606.ENSP00000359504

Structurei

Secondary structure

1592
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32455
SMRiP32455
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32455

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 278GB1/RHD3-type GAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 311GTPase domain (Globular)Add BLAST311

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2037 Eukaryota
ENOG410XR6Z LUCA
GeneTreeiENSGT00550000074475
HOGENOMiHOG000266974
HOVERGENiHBG001979
InParanoidiP32455
KOiK20899
OMAiMAGLYRT
OrthoDBiEOG091G03P3
PhylomeDBiP32455
TreeFamiTF331602

Family and domain databases

CDDicd16269 GBP_C, 1 hit
InterProiView protein in InterPro
IPR030386 G_GB1_RHD3_dom
IPR037684 GBP_C
IPR003191 Guanylate-bd/ATL_C
IPR036543 Guanylate-bd_C_sf
IPR015894 Guanylate-bd_N
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF02263 GBP, 1 hit
PF02841 GBP_C, 1 hit
SUPFAMiSSF48340 SSF48340, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51715 G_GB1_RHD3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32455-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASEIHMTGP MCLIENTNGR LMANPEALKI LSAITQPMVV VAIVGLYRTG
60 70 80 90 100
KSYLMNKLAG KKKGFSLGST VQSHTKGIWM WCVPHPKKPG HILVLLDTEG
110 120 130 140 150
LGDVEKGDNQ NDSWIFALAV LLSSTFVYNS IGTINQQAMD QLYYVTELTH
160 170 180 190 200
RIRSKSSPDE NENEVEDSAD FVSFFPDFVW TLRDFSLDLE ADGQPLTPDE
210 220 230 240 250
YLTYSLKLKK GTSQKDETFN LPRLCIRKFF PKKKCFVFDR PVHRRKLAQL
260 270 280 290 300
EKLQDEELDP EFVQQVADFC SYIFSNSKTK TLSGGIQVNG PRLESLVLTY
310 320 330 340 350
VNAISSGDLP CMENAVLALA QIENSAAVQK AIAHYEQQMG QKVQLPTETL
360 370 380 390 400
QELLDLHRDS EREAIEVFIR SSFKDVDHLF QKELAAQLEK KRDDFCKQNQ
410 420 430 440 450
EASSDRCSAL LQVIFSPLEE EVKAGIYSKP GGYRLFVQKL QDLKKKYYEE
460 470 480 490 500
PRKGIQAEEI LQTYLKSKES MTDAILQTDQ TLTEKEKEIE VERVKAESAQ
510 520 530 540 550
ASAKMLQEMQ RKNEQMMEQK ERSYQEHLKQ LTEKMENDRV QLLKEQERTL
560 570 580 590
ALKLQEQEQL LKEGFQKESR IMKNEIQDLQ TKMRRRKACT IS
Length:592
Mass (Da):67,931
Last modified:November 2, 2010 - v2
Checksum:i3A06741218360732
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03395078I → V. Corresponds to variant dbSNP:rs1048401Ensembl.1
Natural variantiVAR_033951166E → D. Corresponds to variant dbSNP:rs17130717Ensembl.1
Natural variantiVAR_014849349T → S3 PublicationsCorresponds to variant dbSNP:rs1048425Ensembl.1
Natural variantiVAR_046550409A → G3 PublicationsCorresponds to variant dbSNP:rs1048443Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55542 mRNA Translation: AAA35871.1
BT006847 mRNA Translation: AAP35493.1
AK291783 mRNA Translation: BAF84472.1
AL160008 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW73148.1
CH471097 Genomic DNA Translation: EAW73150.1
BC002666 mRNA Translation: AAH02666.1
CCDSiCCDS718.1
PIRiA41268
RefSeqiNP_002044.2, NM_002053.2
UniGeneiHs.62661

Genome annotation databases

EnsembliENST00000370473; ENSP00000359504; ENSG00000117228
GeneIDi2633
KEGGihsa:2633
UCSCiuc001dmx.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55542 mRNA Translation: AAA35871.1
BT006847 mRNA Translation: AAP35493.1
AK291783 mRNA Translation: BAF84472.1
AL160008 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW73148.1
CH471097 Genomic DNA Translation: EAW73150.1
BC002666 mRNA Translation: AAH02666.1
CCDSiCCDS718.1
PIRiA41268
RefSeqiNP_002044.2, NM_002053.2
UniGeneiHs.62661

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DG3X-ray1.80A1-592[»]
1F5NX-ray1.70A1-592[»]
2B8WX-ray2.22A/B1-317[»]
2B92X-ray3.20A/B1-317[»]
2BC9X-ray2.80A1-317[»]
2D4HX-ray2.90A/B1-317[»]
ProteinModelPortaliP32455
SMRiP32455
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108903, 20 interactors
DIPiDIP-60423N
IntActiP32455, 2 interactors
MINTiP32455
STRINGi9606.ENSP00000359504

PTM databases

iPTMnetiP32455
PhosphoSitePlusiP32455
SwissPalmiP32455

Polymorphism and mutation databases

BioMutaiGBP1
DMDMi311033383

Proteomic databases

EPDiP32455
MaxQBiP32455
PaxDbiP32455
PeptideAtlasiP32455
PRIDEiP32455
ProteomicsDBi54877

Protocols and materials databases

DNASUi2633
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370473; ENSP00000359504; ENSG00000117228
GeneIDi2633
KEGGihsa:2633
UCSCiuc001dmx.3 human

Organism-specific databases

CTDi2633
DisGeNETi2633
EuPathDBiHostDB:ENSG00000117228.9
GeneCardsiGBP1
H-InvDBiHIX0018119
HIX0200036
HGNCiHGNC:4182 GBP1
HPAiCAB015450
MIMi600411 gene
neXtProtiNX_P32455
OpenTargetsiENSG00000117228
PharmGKBiPA28596
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2037 Eukaryota
ENOG410XR6Z LUCA
GeneTreeiENSGT00550000074475
HOGENOMiHOG000266974
HOVERGENiHBG001979
InParanoidiP32455
KOiK20899
OMAiMAGLYRT
OrthoDBiEOG091G03P3
PhylomeDBiP32455
TreeFamiTF331602

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling

Miscellaneous databases

ChiTaRSiGBP1 human
EvolutionaryTraceiP32455
GeneWikiiGBP1
GenomeRNAii2633
PROiPR:P32455
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117228 Expressed in 222 organ(s), highest expression level in upper lobe of lung
CleanExiHS_GBP1
GenevisibleiP32455 HS

Family and domain databases

CDDicd16269 GBP_C, 1 hit
InterProiView protein in InterPro
IPR030386 G_GB1_RHD3_dom
IPR037684 GBP_C
IPR003191 Guanylate-bd/ATL_C
IPR036543 Guanylate-bd_C_sf
IPR015894 Guanylate-bd_N
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF02263 GBP, 1 hit
PF02841 GBP_C, 1 hit
SUPFAMiSSF48340 SSF48340, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51715 G_GB1_RHD3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGBP1_HUMAN
AccessioniPrimary (citable) accession number: P32455
Secondary accession number(s): D3DT26, Q5T8M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 2, 2010
Last modified: November 7, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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