UniProtKB - P32455 (GBP1_HUMAN)
Protein
Guanylate-binding protein 1
Gene
GBP1
Organism
Homo sapiens (Human)
Status
Functioni
Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.1 Publication
Catalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | GTP | 8 | |
Nucleotide bindingi | 67 – 69 | GTP | 3 | |
Nucleotide bindingi | 97 – 101 | GTP | 5 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- cytokine binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- GDP binding Source: CAFA
- GTPase activity Source: CAFA
- GTP binding Source: CAFA
- Hsp90 protein binding Source: UniProtKB
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
- spectrin binding Source: UniProtKB
GO - Biological processi
- cellular response to interferon-gamma Source: UniProtKB
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to tumor necrosis factor Source: UniProtKB
- defense response to virus Source: UniProtKB-KW
- interferon-gamma-mediated signaling pathway Source: Reactome
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of interleukin-2 production Source: UniProtKB
- negative regulation of protein localization to plasma membrane Source: UniProtKB
- negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
- negative regulation of T cell receptor signaling pathway Source: UniProtKB
- protein localization to vacuole Source: UniProtKB
- regulation of calcium-mediated signaling Source: UniProtKB
- regulation of protein localization to plasma membrane Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Antiviral defense, Immunity |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P32455 |
Reactomei | R-HSA-877300, Interferon gamma signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Guanylate-binding protein 1 (EC:3.6.5.-2 Publications)Alternative name(s): GTP-binding protein 1 Short name: GBP-1 Short name: HuGBP-1 Guanine nucleotide-binding protein 1 Interferon-induced guanylate-binding protein 1 |
Gene namesi | Name:GBP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000117228.9 |
HGNCi | HGNC:4182, GBP1 |
MIMi | 600411, gene |
neXtProti | NX_P32455 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Extracellular region or secreted
Golgi apparatus
- Golgi apparatus membrane ; Lipid-anchor 1 Publication; Cytoplasmic side
Other locations
- Cytoplasm 1 Publication
Note: Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.
Cytoskeleton
- actin cytoskeleton Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: GO_Central
- vesicle membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Golgi apparatus, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | K → A: Loss of GTPase activity. Constitutively monomeric. Expressed throughout the cytoplasm, loss of vesicular accumulation. 1 Publication | 1 | |
Mutagenesisi | 227 – 228 | RK → EE: Constitutively dimeric. Localizes at vesicle-like structures at the plasma membrane. 1 Publication | 2 | |
Mutagenesisi | 589 – 592 | Missing : Loss of association with membranes. 1 Publication | 4 |
Organism-specific databases
DisGeNETi | 2633 |
OpenTargetsi | ENSG00000117228 |
PharmGKBi | PA28596 |
Miscellaneous databases
Pharosi | P32455, Tbio |
Polymorphism and mutation databases
BioMutai | GBP1 |
DMDMi | 311033383 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190963 | 1 – 589 | Guanylate-binding protein 1Add BLAST | 589 | |
PropeptideiPRO_0000396777 | 590 – 592 | Removed in mature form | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 589 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 589 | S-farnesyl cysteine1 Publication | 1 |
Post-translational modificationi
Isoprenylation is required for proper subcellular location.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | P32455 |
jPOSTi | P32455 |
MassIVEi | P32455 |
MaxQBi | P32455 |
PaxDbi | P32455 |
PeptideAtlasi | P32455 |
PRIDEi | P32455 |
ProteomicsDBi | 54877 |
PTM databases
iPTMneti | P32455 |
MetOSitei | P32455 |
PhosphoSitePlusi | P32455 |
SwissPalmi | P32455 |
Expressioni
Inductioni
By IFNG during macrophage activation, and by TNF and IL1B.1 Publication
Gene expression databases
Bgeei | ENSG00000117228, Expressed in upper lobe of lung and 234 other tissues |
Genevisiblei | P32455, HS |
Organism-specific databases
HPAi | ENSG00000117228, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.
3 PublicationsBinary interactionsi
P32455
With | #Exp. | IntAct |
---|---|---|
itself | 9 | EBI-2869161,EBI-2869161 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- cytokine binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- Hsp90 protein binding Source: UniProtKB
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
- spectrin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 108903, 22 interactors |
DIPi | DIP-60423N |
IntActi | P32455, 11 interactors |
MINTi | P32455 |
STRINGi | 9606.ENSP00000359504 |
Miscellaneous databases
RNActi | P32455, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P32455 |
SMRi | P32455 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32455 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 278 | GB1/RHD3-type GPROSITE-ProRule annotationAdd BLAST | 244 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 311 | GTPase domain (Globular)Add BLAST | 311 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. GB1 subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG2037, Eukaryota |
GeneTreei | ENSGT00940000156840 |
HOGENOMi | CLU_018608_2_2_1 |
InParanoidi | P32455 |
OMAi | KSSMLNW |
OrthoDBi | 1027269at2759 |
PhylomeDBi | P32455 |
TreeFami | TF331602 |
Family and domain databases
CDDi | cd16269, GBP_C, 1 hit |
InterProi | View protein in InterPro IPR030386, G_GB1_RHD3_dom IPR037684, GBP_C IPR003191, Guanylate-bd/ATL_C IPR036543, Guanylate-bd_C_sf IPR015894, Guanylate-bd_N IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02263, GBP, 1 hit PF02841, GBP_C, 1 hit |
SUPFAMi | SSF48340, SSF48340, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51715, G_GB1_RHD3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32455-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASEIHMTGP MCLIENTNGR LMANPEALKI LSAITQPMVV VAIVGLYRTG
60 70 80 90 100
KSYLMNKLAG KKKGFSLGST VQSHTKGIWM WCVPHPKKPG HILVLLDTEG
110 120 130 140 150
LGDVEKGDNQ NDSWIFALAV LLSSTFVYNS IGTINQQAMD QLYYVTELTH
160 170 180 190 200
RIRSKSSPDE NENEVEDSAD FVSFFPDFVW TLRDFSLDLE ADGQPLTPDE
210 220 230 240 250
YLTYSLKLKK GTSQKDETFN LPRLCIRKFF PKKKCFVFDR PVHRRKLAQL
260 270 280 290 300
EKLQDEELDP EFVQQVADFC SYIFSNSKTK TLSGGIQVNG PRLESLVLTY
310 320 330 340 350
VNAISSGDLP CMENAVLALA QIENSAAVQK AIAHYEQQMG QKVQLPTETL
360 370 380 390 400
QELLDLHRDS EREAIEVFIR SSFKDVDHLF QKELAAQLEK KRDDFCKQNQ
410 420 430 440 450
EASSDRCSAL LQVIFSPLEE EVKAGIYSKP GGYRLFVQKL QDLKKKYYEE
460 470 480 490 500
PRKGIQAEEI LQTYLKSKES MTDAILQTDQ TLTEKEKEIE VERVKAESAQ
510 520 530 540 550
ASAKMLQEMQ RKNEQMMEQK ERSYQEHLKQ LTEKMENDRV QLLKEQERTL
560 570 580 590
ALKLQEQEQL LKEGFQKESR IMKNEIQDLQ TKMRRRKACT IS
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033950 | 78 | I → V. Corresponds to variant dbSNP:rs1048401Ensembl. | 1 | |
Natural variantiVAR_033951 | 166 | E → D. Corresponds to variant dbSNP:rs17130717Ensembl. | 1 | |
Natural variantiVAR_014849 | 349 | T → S3 PublicationsCorresponds to variant dbSNP:rs1048425Ensembl. | 1 | |
Natural variantiVAR_046550 | 409 | A → G3 PublicationsCorresponds to variant dbSNP:rs1048443Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55542 mRNA Translation: AAA35871.1 BT006847 mRNA Translation: AAP35493.1 AK291783 mRNA Translation: BAF84472.1 AL160008 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW73148.1 CH471097 Genomic DNA Translation: EAW73150.1 BC002666 mRNA Translation: AAH02666.1 |
CCDSi | CCDS718.1 |
PIRi | A41268 |
RefSeqi | NP_002044.2, NM_002053.2 |
Genome annotation databases
Ensembli | ENST00000370473; ENSP00000359504; ENSG00000117228 |
GeneIDi | 2633 |
KEGGi | hsa:2633 |
UCSCi | uc001dmx.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55542 mRNA Translation: AAA35871.1 BT006847 mRNA Translation: AAP35493.1 AK291783 mRNA Translation: BAF84472.1 AL160008 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW73148.1 CH471097 Genomic DNA Translation: EAW73150.1 BC002666 mRNA Translation: AAH02666.1 |
CCDSi | CCDS718.1 |
PIRi | A41268 |
RefSeqi | NP_002044.2, NM_002053.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DG3 | X-ray | 1.80 | A | 1-592 | [»] | |
1F5N | X-ray | 1.70 | A | 1-592 | [»] | |
2B8W | X-ray | 2.22 | A/B | 1-317 | [»] | |
2B92 | X-ray | 3.20 | A/B | 1-317 | [»] | |
2BC9 | X-ray | 2.80 | A | 1-317 | [»] | |
2D4H | X-ray | 2.90 | A/B | 1-317 | [»] | |
6K1Z | X-ray | 2.31 | A | 1-592 | [»] | |
6K2D | X-ray | 3.60 | A | 1-479 | [»] | |
SASBDBi | P32455 | |||||
SMRi | P32455 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108903, 22 interactors |
DIPi | DIP-60423N |
IntActi | P32455, 11 interactors |
MINTi | P32455 |
STRINGi | 9606.ENSP00000359504 |
PTM databases
iPTMneti | P32455 |
MetOSitei | P32455 |
PhosphoSitePlusi | P32455 |
SwissPalmi | P32455 |
Polymorphism and mutation databases
BioMutai | GBP1 |
DMDMi | 311033383 |
Proteomic databases
EPDi | P32455 |
jPOSTi | P32455 |
MassIVEi | P32455 |
MaxQBi | P32455 |
PaxDbi | P32455 |
PeptideAtlasi | P32455 |
PRIDEi | P32455 |
ProteomicsDBi | 54877 |
Protocols and materials databases
Antibodypediai | 4224, 301 antibodies |
DNASUi | 2633 |
Genome annotation databases
Ensembli | ENST00000370473; ENSP00000359504; ENSG00000117228 |
GeneIDi | 2633 |
KEGGi | hsa:2633 |
UCSCi | uc001dmx.3, human |
Organism-specific databases
CTDi | 2633 |
DisGeNETi | 2633 |
EuPathDBi | HostDB:ENSG00000117228.9 |
GeneCardsi | GBP1 |
HGNCi | HGNC:4182, GBP1 |
HPAi | ENSG00000117228, Low tissue specificity |
MIMi | 600411, gene |
neXtProti | NX_P32455 |
OpenTargetsi | ENSG00000117228 |
PharmGKBi | PA28596 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2037, Eukaryota |
GeneTreei | ENSGT00940000156840 |
HOGENOMi | CLU_018608_2_2_1 |
InParanoidi | P32455 |
OMAi | KSSMLNW |
OrthoDBi | 1027269at2759 |
PhylomeDBi | P32455 |
TreeFami | TF331602 |
Enzyme and pathway databases
PathwayCommonsi | P32455 |
Reactomei | R-HSA-877300, Interferon gamma signaling |
Miscellaneous databases
BioGRID-ORCSi | 2633, 6 hits in 842 CRISPR screens |
ChiTaRSi | GBP1, human |
EvolutionaryTracei | P32455 |
GeneWikii | GBP1 |
GenomeRNAii | 2633 |
Pharosi | P32455, Tbio |
PROi | PR:P32455 |
RNActi | P32455, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117228, Expressed in upper lobe of lung and 234 other tissues |
Genevisiblei | P32455, HS |
Family and domain databases
CDDi | cd16269, GBP_C, 1 hit |
InterProi | View protein in InterPro IPR030386, G_GB1_RHD3_dom IPR037684, GBP_C IPR003191, Guanylate-bd/ATL_C IPR036543, Guanylate-bd_C_sf IPR015894, Guanylate-bd_N IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02263, GBP, 1 hit PF02841, GBP_C, 1 hit |
SUPFAMi | SSF48340, SSF48340, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51715, G_GB1_RHD3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBP1_HUMAN | |
Accessioni | P32455Primary (citable) accession number: P32455 Secondary accession number(s): D3DT26, Q5T8M1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | November 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations