UniProtKB - P32356 (TREA_YEAST)
Protein
Neutral trehalase
Gene
NTH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 1840 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.2.1.28
Activity regulationi
Activated by cAMP-dependent phosphorylation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 302 | SubstrateBy similarity | 1 | |
Binding sitei | 346 | SubstrateBy similarity | 1 | |
Binding sitei | 355 | SubstrateBy similarity | 1 | |
Binding sitei | 476 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 478 | Proton donor/acceptorBy similarity | 1 | |
Active sitei | 674 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- alpha,alpha-trehalase activity Source: SGD
- calcium ion binding Source: InterPro
GO - Biological processi
- cellular response to desiccation Source: SGD
- trehalose catabolic process Source: SGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | MetaCyc:YDR001C-MONOMER |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
Names & Taxonomyi
Protein namesi | Recommended name: Neutral trehalase (EC:3.2.1.28)Alternative name(s): Alpha,alpha-trehalase Alpha,alpha-trehalose glucohydrolase |
Gene namesi | Name:NTH1 Synonyms:NTH Ordered Locus Names:YDR001C ORF Names:YD8119.07C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDR001C |
SGDi | S000002408, NTH1 |
Subcellular locationi
Other locations
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000173798 | 2 – 751 | Neutral trehalaseAdd BLAST | 750 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 21 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 60 | PhosphoserineCombined sources | 1 | |
Modified residuei | 66 | PhosphoserineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P32356 |
PaxDbi | P32356 |
PRIDEi | P32356 |
PTM databases
iPTMneti | P32356 |
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
Hide detailsP32356
With | #Exp. | IntAct |
---|---|---|
BMH1 [P29311] | 7 | EBI-19509,EBI-3661 |
BMH2 [P34730] | 8 | EBI-19509,EBI-3672 |
DCS1 [Q06151] | 3 | EBI-19509,EBI-38973 |
Protein-protein interaction databases
BioGRIDi | 32054, 94 interactors |
DIPi | DIP-1479N |
IntActi | P32356, 16 interactors |
MINTi | P32356 |
STRINGi | 4932.YDR001C |
Miscellaneous databases
RNActi | P32356, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32356 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 309 – 310 | Substrate bindingBy similarity | 2 | |
Regioni | 355 – 357 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 37 family.Curated
Phylogenomic databases
eggNOGi | KOG0602, Eukaryota |
GeneTreei | ENSGT00390000006949 |
HOGENOMi | CLU_006451_1_1_1 |
InParanoidi | P32356 |
OMAi | PFGWAPQ |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS IPR011120, Trehalase_Ca-bd |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit PF07492, Trehalase_Ca-bi, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32356-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQVNTSQGP VAQGRQRRLS SLSEFNDPFS NAEVYYGPPT DPRKQKQAKP
60 70 80 90 100
AKINRTRTMS VFDNVSPFKK TGFGKLQQTR RGSEDDTYSS SQGNRRFFIE
110 120 130 140 150
DVDKTLNELL AAEDTDKNYQ ITIEDTGPKV LKVGTANSYG YKHINIRGTY
160 170 180 190 200
MLSNLLQELT IAKSFGRHQI FLDEARINEN PVNRLSRLIN TQFWNSLTRR
210 220 230 240 250
VDLNNVGEIA KDTKIDTPGA KNPRIYVPYD CPEQYEFYVQ ASQMHPSLKL
260 270 280 290 300
EVEYLPKKIT AEYVKSVNDT PGLLALAMEE HFNPSTGEKT LIGYPYAVPG
310 320 330 340 350
GRFNELYGWD SYMMALGLLE ANKTDVARGM VEHFIFEINH YGKILNANRS
360 370 380 390 400
YYLCRSQPPF LTEMALVVFK KLGGRSNPDA VDLLKRAFQA SIKEYKTVWT
410 420 430 440 450
ASPRLDPETG LSRYHPNGLG IPPETESDHF DTVLLPYASK HGVTLDEFKQ
460 470 480 490 500
LYNDGKIKEP KLDEFFLHDR GVRESGHDTT YRFEGVCAYL ATIDLNSLLY
510 520 530 540 550
KYEIDIADFI KEFCDDKYED PLDHSITTSA MWKEMAKIRQ EKITKYMWDD
560 570 580 590 600
ESGFFFDYNT KIKHRTSYES ATTFWALWAG LATKEQAQKM VEKALPKLEM
610 620 630 640 650
LGGLAACTER SRGPISISRP IRQWDYPFGW APHQILAWEG LRSYGYLTVT
660 670 680 690 700
NRLAYRWLFM MTKAFVDYNG IVVEKYDVTR GTDPHRVEAE YGNQGADFKG
710 720 730 740 750
AATEGFGWVN ASYILGLKYM NSHARRALGA CIPPISFFSS LRPQERNLYG
L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 514 – 515 | CD → NN in AAA66896 (PubMed:3537685).Curated | 2 | |
Sequence conflicti | 712 | S → R in CAA46718 (PubMed:8444853).Curated | 1 | |
Sequence conflicti | 712 | S → R in AAA66896 (PubMed:3537685).Curated | 1 | |
Sequence conflicti | 723 – 724 | HA → YE in CAA46718 (PubMed:8444853).Curated | 2 | |
Sequence conflicti | 723 – 724 | HA → YE in AAA66896 (PubMed:3537685).Curated | 2 | |
Sequence conflicti | 727 – 728 | AL → EI in CAA46718 (PubMed:8444853).Curated | 2 | |
Sequence conflicti | 727 – 728 | AL → EI in AAA66896 (PubMed:3537685).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65925 Genomic DNA Translation: CAA46718.1 Z48008 Genomic DNA Translation: CAA88061.1 M13000 Genomic DNA Translation: AAA66896.1 BK006938 Genomic DNA Translation: DAA11848.1 |
PIRi | S50982 |
RefSeqi | NP_010284.1, NM_001180309.1 |
Genome annotation databases
EnsemblFungii | YDR001C_mRNA; YDR001C; YDR001C |
GeneIDi | 851564 |
KEGGi | sce:YDR001C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65925 Genomic DNA Translation: CAA46718.1 Z48008 Genomic DNA Translation: CAA88061.1 M13000 Genomic DNA Translation: AAA66896.1 BK006938 Genomic DNA Translation: DAA11848.1 |
PIRi | S50982 |
RefSeqi | NP_010284.1, NM_001180309.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5JTA | X-ray | 2.72 | A | 1-751 | [»] | |
5M4A | X-ray | 2.90 | A | 153-751 | [»] | |
5N6N | X-ray | 2.29 | C | 1-751 | [»] | |
5NIS | X-ray | 3.15 | A | 100-751 | [»] | |
SMRi | P32356 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32054, 94 interactors |
DIPi | DIP-1479N |
IntActi | P32356, 16 interactors |
MINTi | P32356 |
STRINGi | 4932.YDR001C |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
PTM databases
iPTMneti | P32356 |
Proteomic databases
MaxQBi | P32356 |
PaxDbi | P32356 |
PRIDEi | P32356 |
Genome annotation databases
EnsemblFungii | YDR001C_mRNA; YDR001C; YDR001C |
GeneIDi | 851564 |
KEGGi | sce:YDR001C |
Organism-specific databases
EuPathDBi | FungiDB:YDR001C |
SGDi | S000002408, NTH1 |
Phylogenomic databases
eggNOGi | KOG0602, Eukaryota |
GeneTreei | ENSGT00390000006949 |
HOGENOMi | CLU_006451_1_1_1 |
InParanoidi | P32356 |
OMAi | PFGWAPQ |
Enzyme and pathway databases
BioCyci | MetaCyc:YDR001C-MONOMER |
Miscellaneous databases
PROi | PR:P32356 |
RNActi | P32356, protein |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS IPR011120, Trehalase_Ca-bd |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit PF07492, Trehalase_Ca-bi, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TREA_YEAST | |
Accessioni | P32356Primary (citable) accession number: P32356 Secondary accession number(s): D6VRY8, E9P9U7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries