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Protein

DNA-directed RNA polymerase III subunit RPC3

Gene

RPC82

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.

Miscellaneous

Present with 2520 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription
LigandZinc

Enzyme and pathway databases

BioCyciYEAST:G3O-34313-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC3
Short name:
RNA polymerase III subunit C3
Alternative name(s):
C82
DNA-directed III 74 kDa polypeptide
Short name:
C74
Gene namesi
Name:RPC82
Synonyms:RPC3
Ordered Locus Names:YPR190C
ORF Names:P9677.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR190C
SGDiS000006394 RPC82

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739661 – 654DNA-directed RNA polymerase III subunit RPC3Add BLAST654

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27PhosphothreonineCombined sources1
Modified residuei392PhosphoserineCombined sources1
Modified residuei394PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32349
PaxDbiP32349
PRIDEiP32349

PTM databases

iPTMnetiP32349

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPC34P329103EBI-15821,EBI-15835

Protein-protein interaction databases

BioGridi36362, 602 interactors
ComplexPortaliCPX-2660 DNA-directed RNA polymerase III complex
DIPiDIP-934N
IntActiP32349, 22 interactors
MINTiP32349
STRINGi4932.YPR190C

Structurei

Secondary structure

1654
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32349
SMRiP32349
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni581 – 602Leucine-zipperAdd BLAST22

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000002799
HOGENOMiHOG000066017
InParanoidiP32349
KOiK03023
OMAiGQWTVDF
OrthoDBiEOG092C1SNJ

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR013197 RNA_pol_III_RPC82-rel_HTH
IPR008806 RNA_pol_III_Rpc82_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF08221 HTH_9, 1 hit
PF05645 RNA_pol_Rpc82, 1 hit

Sequencei

Sequence statusi: Complete.

P32349-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDELLGEALS AENQTGESTV ESEKLVTPED VMTISSLEQR TLNPDLFLYK
60 70 80 90 100
ELVKAHLGER AASVIGMLVA LGRLSVRELV EKIDGMDVDS VKTTLVSLTQ
110 120 130 140 150
LRCVKYLQET AISGKKTTYY YYNEEGIHIL LYSGLIIDEI ITQMRVNDEE
160 170 180 190 200
EHKQLVAEIV QNVISLGSLT VEDYLSSVTS DSMKYTISSL FVQLCEMGYL
210 220 230 240 250
IQISKLHYTP IEDLWQFLYE KHYKNIPRNS PLSDLKKRSQ AKMNAKTDFA
260 270 280 290 300
KIINKPNELS QILTVDPKTS LRIVKPTVSL TINLDRFMKG RRSKQLINLA
310 320 330 340 350
KTRVGSVTAQ VYKIALRLTE QKSPKIRDPL TQTGLLQDLE EAKSFQDEAE
360 370 380 390 400
LVEEKTPGLT FNAIDLARHL PAELDLRGSL LSRKPSDNKK RSGSNAAASL
410 420 430 440 450
PSKKLKTEDG FVIPALPAAV SKSLQESGDT QEEDEEEEDL DADTEDPHSA
460 470 480 490 500
SLINSHLKIL ASSNFPFLNE TKPGVYYVPY SKLMPVLKSS VYEYVIASTL
510 520 530 540 550
GPSAMRLSRC IRDNKLVSEK IINSTALMKE KDIRSTLASL IRYNSVEIQE
560 570 580 590 600
VPRTADRSAS RAVFLFRCKE THSYNFMRQN LEWNMANLLF KKEKLKQENS
610 620 630 640 650
TLLKKANRDD VKGRENELLL PSELNQLKMV NERELNVFAR LSRLLSLWEV

FQMA
Length:654
Mass (Da):74,017
Last modified:December 15, 1998 - v2
Checksum:iB39FA74F4A18BADE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti637V → L in CAA45072 (PubMed:1406632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63500 Genomic DNA Translation: CAA45072.1
U25841 Genomic DNA Translation: AAB64619.1
BK006949 Genomic DNA Translation: DAA11606.1
PIRiS58820
RefSeqiNP_015516.1, NM_001184287.1

Genome annotation databases

EnsemblFungiiYPR190C; YPR190C; YPR190C
GeneIDi856320
KEGGisce:YPR190C

Similar proteinsi

Entry informationi

Entry nameiRPC3_YEAST
AccessioniPrimary (citable) accession number: P32349
Secondary accession number(s): D6W4J0, Q06591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: September 12, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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