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UniProtKB - P32340 (NDI1_YEAST)
Protein
Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
Gene
NDI1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the oxidation of NADH generated inside the Mitochondrion.
Miscellaneous
Present with 5240 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.6.5.9
Cofactori
FADBy similarityNote: Binds 1 FAD per subunit.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 55 – 85 | FADBy similarityAdd BLAST | 31 | |
Nucleotide bindingi | 229 – 265 | NADBy similarityAdd BLAST | 37 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- NADH dehydrogenase (ubiquinone) activity Source: SGD
GO - Biological processi
- mitochondrial electron transport, NADH to ubiquinone Source: SGD
- NADH oxidation Source: SGD
- positive regulation of apoptotic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NAD, Ubiquinone |
Enzyme and pathway databases
BRENDAi | 1.6.5.9, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial (EC:1.6.5.9)Alternative name(s): Internal NADH dehydrogenase NADH:ubiquinone reductase (non-electrogenic) |
Gene namesi | Name:NDI1 Ordered Locus Names:YML120C ORF Names:YM7056.06C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004589, NDI1 |
VEuPathDBi | FungiDB:YML120C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication
Note: Bound to the mitochondrial inner membrane facing the matrix site.
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | Mitochondrion1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000021793 | 27 – 513 | Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrialAdd BLAST | 487 |
Proteomic databases
MaxQBi | P32340 |
PaxDbi | P32340 |
PRIDEi | P32340 |
2D gel databases
UCD-2DPAGEi | P32340 |
PTM databases
iPTMneti | P32340 |
Interactioni
Binary interactionsi
P32340
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-11961,EBI-11961 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 35084, 78 interactors |
DIPi | DIP-5554N |
IntActi | P32340, 15 interactors |
MINTi | P32340 |
STRINGi | 4932.YML120C |
Miscellaneous databases
RNActi | P32340, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P32340 |
SMRi | P32340 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the NADH dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2495, Eukaryota |
HOGENOMi | CLU_021377_1_0_1 |
InParanoidi | P32340 |
OMAi | PYGTLIW |
Family and domain databases
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR045024, NDH-2 |
PANTHERi | PTHR43706, PTHR43706, 1 hit |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32340-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH
60 70 80 90 100
SDKPNVLILG SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG
110 120 130 140 150
TVDEKSIIEP IVNFALKKKG NVTYYEAEAT SINPDRNTVT IKSLSAVSQL
160 170 180 190 200
YQPENHLGLH QAEPAEIKYD YLISAVGAEP NTFGIPGVTD YGHFLKEIPN
210 220 230 240 250
SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG VEAAGELQDY
260 270 280 290 300
VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV
310 320 330 340 350
HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL
360 370 380 390 400
FKKIPEQNSS KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA
410 420 430 440 450
EYLAKNFDKM AQIPNFQKNL SSRKDKIDLL FEENNFKPFK YNDLGALAYL
460 470 480 490 500
GSERAIATIR SGKRTFYTGG GLMTFYLWRI LYLSMILSAR SRLKVFFDWI
510
KLAFFKRDFF KGL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 10 | K → R. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61590 Genomic DNA Translation: CAA43787.1 Sequence problems. Z49218 Genomic DNA Translation: CAA89160.1 AY723851 Genomic DNA Translation: AAU09768.1 BK006946 Genomic DNA Translation: DAA09778.1 |
PIRi | S26704 |
RefSeqi | NP_013586.1, NM_001182483.1 |
Genome annotation databases
EnsemblFungii | YML120C_mRNA; YML120C; YML120C |
GeneIDi | 854919 |
KEGGi | sce:YML120C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61590 Genomic DNA Translation: CAA43787.1 Sequence problems. Z49218 Genomic DNA Translation: CAA89160.1 AY723851 Genomic DNA Translation: AAU09768.1 BK006946 Genomic DNA Translation: DAA09778.1 |
PIRi | S26704 |
RefSeqi | NP_013586.1, NM_001182483.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4G6G | X-ray | 2.39 | A/B | 24-513 | [»] | |
4G6H | X-ray | 2.26 | A/B | 24-513 | [»] | |
4G73 | X-ray | 2.52 | A/B | 24-513 | [»] | |
4G74 | X-ray | 2.48 | A/B | 24-513 | [»] | |
4G9K | X-ray | 2.70 | A/B | 43-513 | [»] | |
4GAP | X-ray | 2.90 | A/B | 43-513 | [»] | |
4GAV | X-ray | 3.00 | A/B | 43-513 | [»] | |
5YJW | X-ray | 1.85 | A | 30-513 | [»] | |
5YJX | X-ray | 3.21 | A/B | 28-513 | [»] | |
AlphaFoldDBi | P32340 | |||||
SMRi | P32340 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35084, 78 interactors |
DIPi | DIP-5554N |
IntActi | P32340, 15 interactors |
MINTi | P32340 |
STRINGi | 4932.YML120C |
PTM databases
iPTMneti | P32340 |
2D gel databases
UCD-2DPAGEi | P32340 |
Proteomic databases
MaxQBi | P32340 |
PaxDbi | P32340 |
PRIDEi | P32340 |
Genome annotation databases
EnsemblFungii | YML120C_mRNA; YML120C; YML120C |
GeneIDi | 854919 |
KEGGi | sce:YML120C |
Organism-specific databases
SGDi | S000004589, NDI1 |
VEuPathDBi | FungiDB:YML120C |
Phylogenomic databases
eggNOGi | KOG2495, Eukaryota |
HOGENOMi | CLU_021377_1_0_1 |
InParanoidi | P32340 |
OMAi | PYGTLIW |
Enzyme and pathway databases
BRENDAi | 1.6.5.9, 984 |
Miscellaneous databases
PROi | PR:P32340 |
RNActi | P32340, protein |
Family and domain databases
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR045024, NDH-2 |
PANTHERi | PTHR43706, PTHR43706, 1 hit |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF51905, SSF51905, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NDI1_YEAST | |
Accessioni | P32340Primary (citable) accession number: P32340 Secondary accession number(s): D6W0G4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families