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Protein

Importin subunit beta-3

Gene

PSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for classical and arginine/glycine-rich nuclear localization signals (cNLS and rg-NLS) in cargo substrates (PubMed:15367670). Its predominant cargo substrate seems to be ribosomal proteins and ribosome biogenesis trans- and cis-acting factors (PubMed:9182759, PubMed:9321403, PubMed:15367670). Required for nuclear transport of YAP1, NOP1 and SOF1 (PubMed:11274141, PubMed:15367670). Mediates the nuclear import of histones H3 and H4 (PubMed:11694505). Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo (PubMed:9321403). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication5 Publications
Plays a role in protein secretion.1 Publication

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • NLS-bearing protein import into nucleus Source: GO_Central
  • protein import into nucleus Source: SGD
  • regulation of mitotic nuclear division Source: SGD
  • regulation of protein desumoylation Source: SGD
  • ribosomal protein import into nucleus Source: GO_Central

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32972-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-3Curated
Alternative name(s):
Karyopherin subunit beta-3Curated
Karyopherin-1211 Publication
Protein secretion enhancer 11 Publication
Gene namesi
Name:PSE11 Publication
Synonyms:KAP1211 Publication
Ordered Locus Names:YMR308CImported
ORF Names:YM9952.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004925 PSE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001207742 – 1089Importin subunit beta-3Add BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei830PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32337
PaxDbiP32337
PRIDEiP32337

PTM databases

iPTMnetiP32337

Interactioni

Subunit structurei

Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with RPL25; this interaction is dissociated by binding to Ran-GTP (PubMed:9321403). Interacts with YAP1; this interaction is dissociated by binding to Ran-GTP (PubMed:11274141). Interacts with NOP1; via its rg-NLS (PubMed:15367670). Interacts with SOF1; via its cNLS (PubMed:15367670). Interacts with histones H3 and H4; via their NLS (PubMed:11694505). Interacts with ABF1 (PubMed:15522095).4 Publications

Protein-protein interaction databases

BioGridi35488, 224 interactors
DIPiDIP-1533N
IntActiP32337, 76 interactors
MINTiP32337
STRINGi4932.YMR308C

Structurei

Secondary structure

11089
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32337
SMRiP32337
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati6 – 39HEAT 11 PublicationAdd BLAST34
Repeati44 – 78HEAT 21 PublicationAdd BLAST35
Repeati96 – 129HEAT 31 PublicationAdd BLAST34
Repeati138 – 165HEAT 41 PublicationAdd BLAST28
Repeati175 – 207HEAT 51 PublicationAdd BLAST33
Repeati216 – 252HEAT 61 PublicationAdd BLAST37
Repeati260 – 295HEAT 71 PublicationAdd BLAST36
Repeati304 – 359HEAT 81 PublicationAdd BLAST56
Repeati361 – 395HEAT 91 PublicationAdd BLAST35
Repeati399 – 439HEAT 101 PublicationAdd BLAST41
Repeati441 – 481HEAT 111 PublicationAdd BLAST41
Repeati484 – 524HEAT 121 PublicationAdd BLAST41
Repeati526 – 568HEAT 131 PublicationAdd BLAST43
Repeati571 – 613HEAT 141 PublicationAdd BLAST43
Repeati615 – 689HEAT 151 PublicationAdd BLAST75
Repeati692 – 735HEAT 161 PublicationAdd BLAST44
Repeati742 – 781HEAT 171 PublicationAdd BLAST40
Repeati788 – 849HEAT 181 PublicationAdd BLAST62
Repeati852 – 890HEAT 191 PublicationAdd BLAST39
Repeati898 – 930HEAT 201 PublicationAdd BLAST33
Repeati938 – 978HEAT 211 PublicationAdd BLAST41
Repeati986 – 1017HEAT 221 PublicationAdd BLAST32
Repeati1028 – 1063HEAT 231 PublicationAdd BLAST36
Repeati1066 – 1089HEAT 241 PublicationAdd BLAST24

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074773
HOGENOMiHOG000209725
InParanoidiP32337
KOiK20222
OMAiNCNLPRI
OrthoDBiEOG092C0HC0

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
SUPFAMiSSF48371 SSF48371, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32337-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF
60 70 80 90 100
LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA
110 120 130 140 150
QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS
160 170 180 190 200
GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA
210 220 230 240 250
FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV
260 270 280 290 300
ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC
310 320 330 340 350
KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ
360 370 380 390 400
ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD
410 420 430 440 450
VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR
460 470 480 490 500
ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV
510 520 530 540 550
LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN
560 570 580 590 600
SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS
610 620 630 640 650
YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ
660 670 680 690 700
QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV
710 720 730 740 750
MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA
760 770 780 790 800
SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA
810 820 830 840 850
NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN
860 870 880 890 900
GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA
910 920 930 940 950
FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV
960 970 980 990 1000
DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT
1010 1020 1030 1040 1050
DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG
1060 1070 1080
QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA
Length:1,089
Mass (Da):121,031
Last modified:October 1, 1996 - v2
Checksum:i9C5F7FB5B8824C37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65A → S in CAA77639 (PubMed:1522152).Curated1
Sequence conflicti65A → S in AAA10665 (PubMed:1522152).Curated1
Sequence conflicti1077P → A in CAA77639 (PubMed:1522152).Curated1
Sequence conflicti1077P → A in AAA10665 (PubMed:1522152).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11538 Genomic DNA Translation: CAA77639.1
S45357 Genomic DNA Translation: AAA10665.1
Z49212 Genomic DNA Translation: CAA89141.1
BK006946 Genomic DNA Translation: DAA10209.1
PIRiS53978
RefSeqiNP_014039.1, NM_001182819.1

Genome annotation databases

EnsemblFungiiYMR308C; YMR308C; YMR308C
GeneIDi855356
KEGGisce:YMR308C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11538 Genomic DNA Translation: CAA77639.1
S45357 Genomic DNA Translation: AAA10665.1
Z49212 Genomic DNA Translation: CAA89141.1
BK006946 Genomic DNA Translation: DAA10209.1
PIRiS53978
RefSeqiNP_014039.1, NM_001182819.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W3TX-ray2.90A1-1089[»]
3W3UX-ray2.60A1-1089[»]
3W3VX-ray3.20A1-1089[»]
3W3WX-ray2.20A1-1089[»]
3W3XX-ray2.90A1-1089[»]
3W3YX-ray2.80A1-1089[»]
3W3ZX-ray2.70A1-1089[»]
4ZJ7X-ray2.40A1-1089[»]
5H2VX-ray2.80A1-1089[»]
ProteinModelPortaliP32337
SMRiP32337
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35488, 224 interactors
DIPiDIP-1533N
IntActiP32337, 76 interactors
MINTiP32337
STRINGi4932.YMR308C

PTM databases

iPTMnetiP32337

Proteomic databases

MaxQBiP32337
PaxDbiP32337
PRIDEiP32337

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR308C; YMR308C; YMR308C
GeneIDi855356
KEGGisce:YMR308C

Organism-specific databases

SGDiS000004925 PSE1

Phylogenomic databases

GeneTreeiENSGT00550000074773
HOGENOMiHOG000209725
InParanoidiP32337
KOiK20222
OMAiNCNLPRI
OrthoDBiEOG092C0HC0

Enzyme and pathway databases

BioCyciYEAST:G3O-32972-MONOMER

Miscellaneous databases

PROiPR:P32337

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIMB3_YEAST
AccessioniPrimary (citable) accession number: P32337
Secondary accession number(s): D6W0D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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