UniProtKB - P32337 (IMB3_YEAST)
Importin subunit beta-3
PSE1
Functioni
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for classical and arginine/glycine-rich nuclear localization signals (cNLS and rg-NLS) in cargo substrates (PubMed:15367670).
Its predominant cargo substrate seems to be ribosomal proteins and ribosome biogenesis trans- and cis-acting factors (PubMed:9182759, PubMed:9321403, PubMed:15367670).
Required for nuclear transport of YAP1, NOP1 and SOF1 (PubMed:11274141, PubMed:15367670).
Mediates the nuclear import of histones H3 and H4 (PubMed:11694505).
Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015).
At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo (PubMed:9321403).
The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).
1 Publication5 PublicationsPlays a role in protein secretion.
1 PublicationMiscellaneous
GO - Molecular functioni
- nuclear import signal receptor activity Source: SGD
- nuclear localization sequence binding Source: SGD
GO - Biological processi
- mRNA export from nucleus Source: SGD
- protein import into nucleus Source: SGD
- regulation of mitotic nuclear division Source: SGD
- regulation of protein desumoylation Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit beta-3CuratedAlternative name(s): Karyopherin subunit beta-3Curated Karyopherin-1211 Publication Protein secretion enhancer 11 Publication |
Gene namesi | Ordered Locus Names:YMR308CImported ORF Names:YM9952.10C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004925, PSE1 |
VEuPathDBi | FungiDB:YMR308C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000120774 | 2 – 1089 | Importin subunit beta-3Add BLAST | 1088 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 830 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P32337 |
PaxDbi | P32337 |
PRIDEi | P32337 |
PTM databases
iPTMneti | P32337 |
Interactioni
Subunit structurei
Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403).
Interacts with RPL25; this interaction is dissociated by binding to Ran-GTP (PubMed:9321403).
Interacts with YAP1; this interaction is dissociated by binding to Ran-GTP (PubMed:11274141).
Interacts with NOP1; via its rg-NLS (PubMed:15367670).
Interacts with SOF1; via its cNLS (PubMed:15367670).
Interacts with histones H3 and H4; via their NLS (PubMed:11694505).
Interacts with ABF1 (PubMed:15522095).
4 PublicationsProtein-protein interaction databases
BioGRIDi | 35488, 242 interactors |
DIPi | DIP-1533N |
IntActi | P32337, 76 interactors |
MINTi | P32337 |
STRINGi | 4932.YMR308C |
Miscellaneous databases
RNActi | P32337, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P32337 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 6 – 39 | HEAT 11 PublicationAdd BLAST | 34 | |
Repeati | 44 – 78 | HEAT 21 PublicationAdd BLAST | 35 | |
Repeati | 96 – 129 | HEAT 31 PublicationAdd BLAST | 34 | |
Repeati | 138 – 165 | HEAT 41 PublicationAdd BLAST | 28 | |
Repeati | 175 – 207 | HEAT 51 PublicationAdd BLAST | 33 | |
Repeati | 216 – 252 | HEAT 61 PublicationAdd BLAST | 37 | |
Repeati | 260 – 295 | HEAT 71 PublicationAdd BLAST | 36 | |
Repeati | 304 – 359 | HEAT 81 PublicationAdd BLAST | 56 | |
Repeati | 361 – 395 | HEAT 91 PublicationAdd BLAST | 35 | |
Repeati | 399 – 439 | HEAT 101 PublicationAdd BLAST | 41 | |
Repeati | 441 – 481 | HEAT 111 PublicationAdd BLAST | 41 | |
Repeati | 484 – 524 | HEAT 121 PublicationAdd BLAST | 41 | |
Repeati | 526 – 568 | HEAT 131 PublicationAdd BLAST | 43 | |
Repeati | 571 – 613 | HEAT 141 PublicationAdd BLAST | 43 | |
Repeati | 615 – 689 | HEAT 151 PublicationAdd BLAST | 75 | |
Repeati | 692 – 735 | HEAT 161 PublicationAdd BLAST | 44 | |
Repeati | 742 – 781 | HEAT 171 PublicationAdd BLAST | 40 | |
Repeati | 788 – 849 | HEAT 181 PublicationAdd BLAST | 62 | |
Repeati | 852 – 890 | HEAT 191 PublicationAdd BLAST | 39 | |
Repeati | 898 – 930 | HEAT 201 PublicationAdd BLAST | 33 | |
Repeati | 938 – 978 | HEAT 211 PublicationAdd BLAST | 41 | |
Repeati | 986 – 1017 | HEAT 221 PublicationAdd BLAST | 32 | |
Repeati | 1028 – 1063 | HEAT 231 PublicationAdd BLAST | 36 | |
Repeati | 1066 – 1089 | HEAT 241 PublicationAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2171, Eukaryota |
GeneTreei | ENSGT00940000169161 |
HOGENOMi | CLU_003794_0_1_1 |
InParanoidi | P32337 |
OMAi | AQYLMHC |
Family and domain databases
DisProti | DP02144 |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR040122, Importin_beta IPR041653, Importin_rep_4 IPR040928, Importin_rep_5 IPR041389, Importin_rep_6 |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF18808, Importin_rep_4, 1 hit PF18816, Importin_rep_5, 1 hit PF18829, Importin_rep_6, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF
60 70 80 90 100
LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA
110 120 130 140 150
QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS
160 170 180 190 200
GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA
210 220 230 240 250
FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV
260 270 280 290 300
ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC
310 320 330 340 350
KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ
360 370 380 390 400
ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD
410 420 430 440 450
VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR
460 470 480 490 500
ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV
510 520 530 540 550
LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN
560 570 580 590 600
SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS
610 620 630 640 650
YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ
660 670 680 690 700
QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV
710 720 730 740 750
MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA
760 770 780 790 800
SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA
810 820 830 840 850
NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN
860 870 880 890 900
GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA
910 920 930 940 950
FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV
960 970 980 990 1000
DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT
1010 1020 1030 1040 1050
DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG
1060 1070 1080
QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 65 | A → S in CAA77639 (PubMed:1522152).Curated | 1 | |
Sequence conflicti | 65 | A → S in AAA10665 (PubMed:1522152).Curated | 1 | |
Sequence conflicti | 1077 | P → A in CAA77639 (PubMed:1522152).Curated | 1 | |
Sequence conflicti | 1077 | P → A in AAA10665 (PubMed:1522152).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11538 Genomic DNA Translation: CAA77639.1 S45357 Genomic DNA Translation: AAA10665.1 Z49212 Genomic DNA Translation: CAA89141.1 BK006946 Genomic DNA Translation: DAA10209.1 |
PIRi | S53978 |
RefSeqi | NP_014039.1, NM_001182819.1 |
Genome annotation databases
EnsemblFungii | YMR308C_mRNA; YMR308C; YMR308C |
GeneIDi | 855356 |
KEGGi | sce:YMR308C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11538 Genomic DNA Translation: CAA77639.1 S45357 Genomic DNA Translation: AAA10665.1 Z49212 Genomic DNA Translation: CAA89141.1 BK006946 Genomic DNA Translation: DAA10209.1 |
PIRi | S53978 |
RefSeqi | NP_014039.1, NM_001182819.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3W3T | X-ray | 2.90 | A | 1-1089 | [»] | |
3W3U | X-ray | 2.60 | A | 1-1089 | [»] | |
3W3V | X-ray | 3.20 | A | 1-1089 | [»] | |
3W3W | X-ray | 2.20 | A | 1-1089 | [»] | |
3W3X | X-ray | 2.90 | A | 1-1089 | [»] | |
3W3Y | X-ray | 2.80 | A | 1-1089 | [»] | |
3W3Z | X-ray | 2.70 | A | 1-1089 | [»] | |
4ZJ7 | X-ray | 2.40 | A | 1-1089 | [»] | |
5H2V | X-ray | 2.80 | A | 1-1089 | [»] | |
SMRi | P32337 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35488, 242 interactors |
DIPi | DIP-1533N |
IntActi | P32337, 76 interactors |
MINTi | P32337 |
STRINGi | 4932.YMR308C |
PTM databases
iPTMneti | P32337 |
Proteomic databases
MaxQBi | P32337 |
PaxDbi | P32337 |
PRIDEi | P32337 |
Genome annotation databases
EnsemblFungii | YMR308C_mRNA; YMR308C; YMR308C |
GeneIDi | 855356 |
KEGGi | sce:YMR308C |
Organism-specific databases
SGDi | S000004925, PSE1 |
VEuPathDBi | FungiDB:YMR308C |
Phylogenomic databases
eggNOGi | KOG2171, Eukaryota |
GeneTreei | ENSGT00940000169161 |
HOGENOMi | CLU_003794_0_1_1 |
InParanoidi | P32337 |
OMAi | AQYLMHC |
Miscellaneous databases
PROi | PR:P32337 |
RNActi | P32337, protein |
Family and domain databases
DisProti | DP02144 |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR040122, Importin_beta IPR041653, Importin_rep_4 IPR040928, Importin_rep_5 IPR041389, Importin_rep_6 |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF18808, Importin_rep_4, 1 hit PF18816, Importin_rep_5, 1 hit PF18829, Importin_rep_6, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IMB3_YEAST | |
Accessioni | P32337Primary (citable) accession number: P32337 Secondary accession number(s): D6W0D5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families