UniProtKB - P32325 (DBF4_YEAST)
Protein
DDK kinase regulatory subunit DBF4
Gene
DBF4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK has also postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD.12 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 654 – 703 | DBF4-typePROSITE-ProRule annotationAdd BLAST | 50 |
GO - Molecular functioni
- DNA replication origin binding Source: SGD
- protein serine/threonine kinase activator activity Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-KW
- DNA replication initiation Source: SGD
- mitotic DNA replication checkpoint Source: SGD
- negative regulation of exit from mitosis Source: SGD
- positive regulation of DNA replication initiation Source: SGD
- positive regulation of meiosis I Source: SGD
- positive regulation of nuclear cell cycle DNA replication Source: GO_Central
- positive regulation of protein kinase activity Source: SGD
- positive regulation of protein serine/threonine kinase activity Source: SGD
- premeiotic DNA replication Source: SGD
- regulation of cell cycle phase transition Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, Chromosome partition, DNA replication, Meiosis, Mitosis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-68962, Activation of the pre-replicative complex |
Protein family/group databases
MoonDBi | P32325, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: DDK kinase regulatory subunit DBF4Alternative name(s): Dumbbell forming protein 4 |
Gene namesi | Name:DBF4 Synonyms:DNA52 Ordered Locus Names:YDR052C ORF Names:D4205, YD9609.07C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002459, DBF4 |
VEuPathDBi | FungiDB:YDR052C |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- chromosome, centromeric region Source: SGD
- cytoplasm Source: SGD
- Dbf4-dependent protein kinase complex Source: SGD
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 83 | R → A or E: Defective for interaction with CDC5. 1 Publication | 1 | |
Mutagenesisi | 84 | S → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 85 | I → A: Defective for interaction with CDC5. 1 Publication | 1 | |
Mutagenesisi | 86 | E → K: No effect. 1 Publication | 1 | |
Mutagenesisi | 87 | G → A: Defective for interaction with CDC5. 1 Publication | 1 | |
Mutagenesisi | 88 | A → V: Defective for interaction with CDC5. 1 Publication | 1 | |
Mutagenesisi | 661 | C → A in DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-664. 1 Publication | 1 | |
Mutagenesisi | 664 | C → A in DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-661. 1 Publication | 1 | |
Mutagenesisi | 674 | H → A in DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-680. 1 Publication | 1 | |
Mutagenesisi | 680 | H → A: Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands. In DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-674. 1 Publication | 1 | |
Mutagenesisi | 680 | H → C: Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079791 | 1 – 704 | DDK kinase regulatory subunit DBF4Add BLAST | 704 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 59 | PhosphoserineCombined sources | 1 | |
Modified residuei | 84 | PhosphoserineCombined sources | 1 | |
Modified residuei | 235 | PhosphoserineCombined sources | 1 | |
Modified residuei | 623 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by CDC7 and by CDC5.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32325 |
PaxDbi | P32325 |
PRIDEi | P32325 |
PTM databases
iPTMneti | P32325 |
Expressioni
Inductioni
Cell cycle-regulated. Protein levels increase as cells begin S phase and remain high through late mitosis.2 Publications
Interactioni
Subunit structurei
Heterodimer with CDC7 to form the DBF4-dependent kinase (DDK) complex.
Interacts (via PBD-binding motif) with CDC5 (via POLO box domains).
Interacts (via N-terminus) with ORC2, ORC3 and RAD53. Binds to ARS1 origin DNA.
6 PublicationsBinary interactionsi
P32325
With | #Exp. | IntAct |
---|---|---|
CDC7 [P06243] | 8 | EBI-5575,EBI-4451 |
Protein-protein interaction databases
BioGRIDi | 32106, 824 interactors |
ComplexPortali | CPX-867, DBF4-dependent CDC7 kinase complex |
DIPi | DIP-2290N |
IntActi | P32325, 5 interactors |
MINTi | P32325 |
STRINGi | 4932.YDR052C |
Miscellaneous databases
RNActi | P32325, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32325 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32325 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 10 – 19 | D box 1 | 10 | |
Regioni | 62 – 70 | D box 2 | 9 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 83 – 88 | POLO box domain (PBD)-binding | 6 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 654 – 703 | DBF4-typePROSITE-ProRule annotationAdd BLAST | 50 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4139, Eukaryota |
GeneTreei | ENSGT00530000063909 |
HOGENOMi | CLU_023948_0_0_1 |
InParanoidi | P32325 |
OMAi | PIITLEW |
Family and domain databases
Gene3Di | 3.30.160.680, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR036420, BRCT_dom_sf IPR013939, Regulatory_Dfp1/Him1 IPR006572, Znf_DBF IPR038545, Znf_DBF_sf |
Pfami | View protein in Pfam PF08630, Dfp1_Him1_M, 1 hit PF07535, zf-DBF, 1 hit |
SMARTi | View protein in SMART SM00586, ZnF_DBF, 1 hit |
PROSITEi | View protein in PROSITE PS51265, ZF_DBF4, 1 hit |
i Sequence
Sequence statusi: Complete.
P32325-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSPTKMIIR SPLKETDTNL KHNNGIAAST TAAGHLNVFS NDNNCNNNNT
60 70 80 90 100
TESFPKKRSL ERLELQQQQH LHEKKRARIE RARSIEGAVQ VSKGTGLKNV
110 120 130 140 150
EPRVTPKELL EWQTNWKKIM KRDSRIYFDI TDDVEMNTYN KSKMDKRRDL
160 170 180 190 200
LKRGFLTLGA QITQFFDTTV TIVITRRSVE NIYLLKDTDI LSRAKKNYMK
210 220 230 240 250
VWSYEKAARF LKNLDVDLDH LSKTKSASLA APTLSNLLHN EKLYGPTDRD
260 270 280 290 300
PRTKRDDIHY FKYPHVYLYD LWQTWAPIIT LEWKPQELTN LDELPYPILK
310 320 330 340 350
IGSFGRCPFI GDRNYDESSY KRVVKRYSRD KANKKYALQL RALFQYHADT
360 370 380 390 400
LLNTSSVNDQ TKNLIFIPHT CNDSTKSFKK WMQEKAKNFE KTELKKTDDS
410 420 430 440 450
AVQDVRNEHA DQTDEKNSIL LNETETKEPP LKEEKENKQS IAEESNKYPQ
460 470 480 490 500
RKELAATPKL NHPVLATFAR QETEEVPDDL CTLKTKSRQA FEIKASGAHQ
510 520 530 540 550
SNDVATSFGN GLGPTRASVM SKNMKSLSRL MVDRKLGVKQ TNGNNKNYTA
560 570 580 590 600
TIATTAETSK ENRHRLDFNA LKKDEAPSKE TGKDSAVHLE TNRKPQNFPK
610 620 630 640 650
VATKSVSADS KVHNDIKITT TESPTASKKS TSTNVTLHFN AQTAQTAQPV
660 670 680 690 700
KKETVKNSGY CENCRVKYES LEQHIVSEKH LSFAENDLNF EAIDSLIENL
RFQI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 – 160 | GA → NT in CAA42819 (PubMed:1592236).Curated | 2 | |
Sequence conflicti | 177 | R → RR in CAA42819 (PubMed:1592236).Curated | 1 | |
Sequence conflicti | 197 | N → K in CAA42819 (PubMed:1592236).Curated | 1 | |
Sequence conflicti | 256 | D → G in CAA42819 (PubMed:1592236).Curated | 1 | |
Sequence conflicti | 416 – 425 | Missing in CAA42819 (PubMed:1592236).Curated | 10 | |
Sequence conflicti | 439 | Q → R in CAA42819 (PubMed:1592236).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60279 Genomic DNA Translation: CAA42819.1 M83539 Genomic DNA Translation: AAA34573.1 X84162 Genomic DNA Translation: CAA58969.1 Z49209 Genomic DNA Translation: CAA89082.1 Z74348 Genomic DNA Translation: CAA98869.1 BK006938 Genomic DNA Translation: DAA11899.1 |
PIRi | S25371 |
RefSeqi | NP_010337.3, NM_001180360.3 |
Genome annotation databases
EnsemblFungii | YDR052C_mRNA; YDR052C; YDR052C |
GeneIDi | 851623 |
KEGGi | sce:YDR052C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60279 Genomic DNA Translation: CAA42819.1 M83539 Genomic DNA Translation: AAA34573.1 X84162 Genomic DNA Translation: CAA58969.1 Z49209 Genomic DNA Translation: CAA89082.1 Z74348 Genomic DNA Translation: CAA98869.1 BK006938 Genomic DNA Translation: DAA11899.1 |
PIRi | S25371 |
RefSeqi | NP_010337.3, NM_001180360.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OQ0 | X-ray | 2.70 | A/B/C/D/E/F/G/H/I/J | 120-250 | [»] | |
3OQ4 | X-ray | 2.40 | A/B/C/D/E | 120-250 | [»] | |
3QBZ | X-ray | 2.69 | A | 66-221 | [»] | |
5T2F | X-ray | 2.66 | A/B/C/D | 105-220 | [»] | |
5T2S | X-ray | 2.40 | A/C | 105-220 | [»] | |
6MF6 | X-ray | 3.40 | C/D | 76-96 | [»] | |
SMRi | P32325 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32106, 824 interactors |
ComplexPortali | CPX-867, DBF4-dependent CDC7 kinase complex |
DIPi | DIP-2290N |
IntActi | P32325, 5 interactors |
MINTi | P32325 |
STRINGi | 4932.YDR052C |
Protein family/group databases
MoonDBi | P32325, Predicted |
PTM databases
iPTMneti | P32325 |
Proteomic databases
MaxQBi | P32325 |
PaxDbi | P32325 |
PRIDEi | P32325 |
Genome annotation databases
EnsemblFungii | YDR052C_mRNA; YDR052C; YDR052C |
GeneIDi | 851623 |
KEGGi | sce:YDR052C |
Organism-specific databases
SGDi | S000002459, DBF4 |
VEuPathDBi | FungiDB:YDR052C |
Phylogenomic databases
eggNOGi | KOG4139, Eukaryota |
GeneTreei | ENSGT00530000063909 |
HOGENOMi | CLU_023948_0_0_1 |
InParanoidi | P32325 |
OMAi | PIITLEW |
Enzyme and pathway databases
Reactomei | R-SCE-68962, Activation of the pre-replicative complex |
Miscellaneous databases
EvolutionaryTracei | P32325 |
PROi | PR:P32325 |
RNActi | P32325, protein |
Family and domain databases
Gene3Di | 3.30.160.680, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR036420, BRCT_dom_sf IPR013939, Regulatory_Dfp1/Him1 IPR006572, Znf_DBF IPR038545, Znf_DBF_sf |
Pfami | View protein in Pfam PF08630, Dfp1_Him1_M, 1 hit PF07535, zf-DBF, 1 hit |
SMARTi | View protein in SMART SM00586, ZnF_DBF, 1 hit |
PROSITEi | View protein in PROSITE PS51265, ZF_DBF4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DBF4_YEAST | |
Accessioni | P32325Primary (citable) accession number: P32325 Secondary accession number(s): D6VS39, P32355 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references