UniProtKB - P32321 (DCTD_HUMAN)
Protein
Deoxycytidylate deaminase
Gene
DCTD
Organism
Homo sapiens (Human)
Status
Functioni
Supplies the nucleotide substrate for thymidylate synthetase.
Catalytic activityi
- EC:3.5.4.12
Cofactori
Activity regulationi
Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 84 | Zinc; catalytic1 Publication | 1 | |
Active sitei | 86 | Proton donorBy similarity | 1 | |
Metal bindingi | 110 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 113 | Zinc; catalytic1 Publication | 1 |
GO - Molecular functioni
- dCMP deaminase activity Source: GO_Central
- identical protein binding Source: IntAct
- zinc ion binding Source: InterPro
GO - Biological processi
- dTMP biosynthetic process Source: GO_Central
- dUMP biosynthetic process Source: GO_Central
- nucleobase-containing small molecule interconversion Source: Reactome
- pyrimidine nucleotide metabolic process Source: ProtInc
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase |
Biological process | Nucleotide biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05252-MONOMER |
PathwayCommonsi | P32321 |
Reactomei | R-HSA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P32321 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DCTD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000129187.14 |
HGNCi | HGNC:2710, DCTD |
MIMi | 607638, gene |
neXtProti | NX_P32321 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 1635 |
OpenTargetsi | ENSG00000129187 |
PharmGKBi | PA138 |
Miscellaneous databases
Pharosi | P32321, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5675 |
DrugBanki | DB00987, Cytarabine |
Polymorphism and mutation databases
BioMutai | DCTD |
DMDMi | 23503055 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171691 | 1 – 178 | Deoxycytidylate deaminaseAdd BLAST | 178 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 174 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P32321 |
jPOSTi | P32321 |
MassIVEi | P32321 |
MaxQBi | P32321 |
PaxDbi | P32321 |
PeptideAtlasi | P32321 |
PRIDEi | P32321 |
ProteomicsDBi | 54870 [P32321-1] 54871 [P32321-2] |
PTM databases
iPTMneti | P32321 |
MetOSitei | P32321 |
PhosphoSitePlusi | P32321 |
Expressioni
Gene expression databases
Bgeei | ENSG00000129187, Expressed in stromal cell of endometrium and 237 other tissues |
ExpressionAtlasi | P32321, baseline and differential |
Genevisiblei | P32321, HS |
Organism-specific databases
HPAi | ENSG00000129187, Low tissue specificity |
Interactioni
Subunit structurei
Homohexamer.
1 PublicationBinary interactionsi
Hide detailsP32321
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 108003, 18 interactors |
IntActi | P32321, 14 interactors |
STRINGi | 9606.ENSP00000349576 |
Miscellaneous databases
RNActi | P32321, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32321 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32321 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 145 | CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST | 132 |
Sequence similaritiesi
Belongs to the cytidine and deoxycytidylate deaminase family.Curated
Phylogenomic databases
eggNOGi | KOG3127, Eukaryota |
GeneTreei | ENSGT00940000153676 |
InParanoidi | P32321 |
OMAi | VKDRMII |
OrthoDBi | 1569973at2759 |
PhylomeDBi | P32321 |
TreeFami | TF105971 |
Family and domain databases
CDDi | cd01286, deoxycytidylate_deaminase, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR016473, dCMP_deaminase IPR015517, dCMP_deaminase-rel IPR035105, Deoxycytidylate_deaminase_dom |
PANTHERi | PTHR11086, PTHR11086, 1 hit |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit |
PIRSFi | PIRSF006019, dCMP_deaminase, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P32321-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG
60 70 80 90 100
IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG
110 120 130 140 150
CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV
160 170
TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6R9S0 | D6R9S0_HUMAN | Deoxycytidylate deaminase | DCTD | 119 | Annotation score: | ||
D6RBJ9 | D6RBJ9_HUMAN | Deoxycytidylate deaminase | DCTD | 169 | Annotation score: | ||
D6RBN2 | D6RBN2_HUMAN | Deoxycytidylate deaminase | DCTD | 120 | Annotation score: | ||
D6RAD7 | D6RAD7_HUMAN | Deoxycytidylate deaminase | DCTD | 59 | Annotation score: | ||
D6RAR9 | D6RAR9_HUMAN | Deoxycytidylate deaminase | DCTD | 73 | Annotation score: | ||
D6RC36 | D6RC36_HUMAN | Deoxycytidylate deaminase | DCTD | 98 | Annotation score: | ||
D6RD72 | D6RD72_HUMAN | Deoxycytidylate deaminase | DCTD | 41 | Annotation score: | ||
D6RJA9 | D6RJA9_HUMAN | Deoxycytidylate deaminase | DCTD | 23 | Annotation score: | ||
D6R9P0 | D6R9P0_HUMAN | Deoxycytidylate deaminase | DCTD | 44 | Annotation score: | ||
D6RIG3 | D6RIG3_HUMAN | Deoxycytidylate deaminase | DCTD | 17 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | S → L in AAH01286 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 128 | M → T in AAA35755 (PubMed:7685356).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038094 | 1 | M → MVGGGQPCGPNM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12136 mRNA Translation: AAA35755.1 L39874 Genomic DNA Translation: AAC37579.1 AK313221 mRNA Translation: BAG36033.1 AC079766 Genomic DNA No translation available. CH471056 Genomic DNA Translation: EAX04697.1 CH471056 Genomic DNA Translation: EAX04698.1 CH471056 Genomic DNA Translation: EAX04700.1 CH471056 Genomic DNA Translation: EAX04701.1 BC001286 mRNA Translation: AAH01286.1 BC088357 mRNA Translation: AAH88357.2 |
CCDSi | CCDS34108.1 [P32321-2] CCDS3831.1 [P32321-1] |
PIRi | A47288 I55434 |
RefSeqi | NP_001012750.1, NM_001012732.1 [P32321-2] NP_001912.2, NM_001921.2 [P32321-1] XP_005262836.1, XM_005262779.3 XP_005262837.1, XM_005262780.3 XP_011529976.1, XM_011531674.2 [P32321-1] XP_016863305.1, XM_017007816.1 XP_016863306.1, XM_017007817.1 XP_016863307.1, XM_017007818.1 XP_016863308.1, XM_017007819.1 XP_016863309.1, XM_017007820.1 [P32321-1] XP_016863310.1, XM_017007821.1 XP_016863311.1, XM_017007822.1 [P32321-1] |
Genome annotation databases
Ensembli | ENST00000357067; ENSP00000349576; ENSG00000129187 [P32321-2] ENST00000438320; ENSP00000398194; ENSG00000129187 [P32321-1] ENST00000510370; ENSP00000424017; ENSG00000129187 [P32321-1] |
GeneIDi | 1635 |
KEGGi | hsa:1635 |
UCSCi | uc003ivf.4, human [P32321-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12136 mRNA Translation: AAA35755.1 L39874 Genomic DNA Translation: AAC37579.1 AK313221 mRNA Translation: BAG36033.1 AC079766 Genomic DNA No translation available. CH471056 Genomic DNA Translation: EAX04697.1 CH471056 Genomic DNA Translation: EAX04698.1 CH471056 Genomic DNA Translation: EAX04700.1 CH471056 Genomic DNA Translation: EAX04701.1 BC001286 mRNA Translation: AAH01286.1 BC088357 mRNA Translation: AAH88357.2 |
CCDSi | CCDS34108.1 [P32321-2] CCDS3831.1 [P32321-1] |
PIRi | A47288 I55434 |
RefSeqi | NP_001012750.1, NM_001012732.1 [P32321-2] NP_001912.2, NM_001921.2 [P32321-1] XP_005262836.1, XM_005262779.3 XP_005262837.1, XM_005262780.3 XP_011529976.1, XM_011531674.2 [P32321-1] XP_016863305.1, XM_017007816.1 XP_016863306.1, XM_017007817.1 XP_016863307.1, XM_017007818.1 XP_016863308.1, XM_017007819.1 XP_016863309.1, XM_017007820.1 [P32321-1] XP_016863310.1, XM_017007821.1 XP_016863311.1, XM_017007822.1 [P32321-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2W4L | X-ray | 2.10 | A/B/C/D/E/F | 5-173 | [»] | |
SMRi | P32321 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108003, 18 interactors |
IntActi | P32321, 14 interactors |
STRINGi | 9606.ENSP00000349576 |
Chemistry databases
ChEMBLi | CHEMBL5675 |
DrugBanki | DB00987, Cytarabine |
PTM databases
iPTMneti | P32321 |
MetOSitei | P32321 |
PhosphoSitePlusi | P32321 |
Polymorphism and mutation databases
BioMutai | DCTD |
DMDMi | 23503055 |
Proteomic databases
EPDi | P32321 |
jPOSTi | P32321 |
MassIVEi | P32321 |
MaxQBi | P32321 |
PaxDbi | P32321 |
PeptideAtlasi | P32321 |
PRIDEi | P32321 |
ProteomicsDBi | 54870 [P32321-1] 54871 [P32321-2] |
Protocols and materials databases
Antibodypediai | 28704, 184 antibodies |
DNASUi | 1635 |
Genome annotation databases
Ensembli | ENST00000357067; ENSP00000349576; ENSG00000129187 [P32321-2] ENST00000438320; ENSP00000398194; ENSG00000129187 [P32321-1] ENST00000510370; ENSP00000424017; ENSG00000129187 [P32321-1] |
GeneIDi | 1635 |
KEGGi | hsa:1635 |
UCSCi | uc003ivf.4, human [P32321-1] |
Organism-specific databases
CTDi | 1635 |
DisGeNETi | 1635 |
EuPathDBi | HostDB:ENSG00000129187.14 |
GeneCardsi | DCTD |
HGNCi | HGNC:2710, DCTD |
HPAi | ENSG00000129187, Low tissue specificity |
MIMi | 607638, gene |
neXtProti | NX_P32321 |
OpenTargetsi | ENSG00000129187 |
PharmGKBi | PA138 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3127, Eukaryota |
GeneTreei | ENSGT00940000153676 |
InParanoidi | P32321 |
OMAi | VKDRMII |
OrthoDBi | 1569973at2759 |
PhylomeDBi | P32321 |
TreeFami | TF105971 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05252-MONOMER |
PathwayCommonsi | P32321 |
Reactomei | R-HSA-499943, Interconversion of nucleotide di- and triphosphates |
SABIO-RKi | P32321 |
Miscellaneous databases
BioGRID-ORCSi | 1635, 11 hits in 848 CRISPR screens |
ChiTaRSi | DCTD, human |
EvolutionaryTracei | P32321 |
GenomeRNAii | 1635 |
Pharosi | P32321, Tbio |
PROi | PR:P32321 |
RNActi | P32321, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000129187, Expressed in stromal cell of endometrium and 237 other tissues |
ExpressionAtlasi | P32321, baseline and differential |
Genevisiblei | P32321, HS |
Family and domain databases
CDDi | cd01286, deoxycytidylate_deaminase, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR016473, dCMP_deaminase IPR015517, dCMP_deaminase-rel IPR035105, Deoxycytidylate_deaminase_dom |
PANTHERi | PTHR11086, PTHR11086, 1 hit |
Pfami | View protein in Pfam PF00383, dCMP_cyt_deam_1, 1 hit |
PIRSFi | PIRSF006019, dCMP_deaminase, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCTD_HUMAN | |
Accessioni | P32321Primary (citable) accession number: P32321 Secondary accession number(s): B2R836 Q9BVD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | September 19, 2002 | |
Last modified: | December 2, 2020 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families