UniProtKB - P32318 (THI4_YEAST)
Thiamine thiazole synthase
THI4
Functioni
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
UniRule annotation7 PublicationsMiscellaneous
Catalytic activityi
- [ADP-thiazole synthase]-L-cysteine + glycine + NAD+ = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 2 H+ + 3 H2O + nicotinamideUniRule annotation1 PublicationEC:2.4.2.60UniRule annotation1 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 76 | Substrate; via amide nitrogen | 1 | |
Binding sitei | 105 | Substrate; via amide nitrogen | 1 | |
Binding sitei | 170 | Substrate; via amide nitrogen and carbonyl oxygen | 1 | |
Binding sitei | 207 | Substrate | 1 | |
Binding sitei | 237 | Substrate | 1 | |
Binding sitei | 291 | Substrate; via amide nitrogen | 1 |
GO - Molecular functioni
- ferrous iron binding Source: SGD
- pentosyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- mitochondrial genome maintenance Source: SGD
- thiamine biosynthetic process Source: SGD
- thiazole biosynthetic process Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Thiamine biosynthesis |
Ligand | Iron, Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 2.4.2.60, 984 2.8.1.10, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Thiamine thiazole synthaseUniRule annotation (EC:2.4.2.60UniRule annotation1 Publication)Alternative name(s): Thiazole biosynthetic enzymeUniRule annotation |
Gene namesi | Ordered Locus Names:YGR144W ORF Names:G6620 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003376, THI4 |
VEuPathDBi | FungiDB:YGR144W |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 97 | E → A or Q: No activity. 1 Publication | 1 | |
Mutagenesisi | 200 | H → N: Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction. 1 Publication | 1 | |
Mutagenesisi | 204 | C → A: Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction. 1 Publication | 1 | |
Mutagenesisi | 205 | C → S: Partially active, with very weak activity toward NAD. 1 Publication | 1 | |
Mutagenesisi | 207 | D → A: No activity. 1 Publication | 1 | |
Mutagenesisi | 237 | H → A or N: No activity. 1 Publication | 1 | |
Mutagenesisi | 238 | D → A: Partially active, with very weak activity toward NAD. 1 Publication | 1 | |
Mutagenesisi | 301 | R → A or Q: No activity. 1 Publication | 1 | |
Mutagenesisi | 304 | P → A: No activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000034058 | 1 – 326 | Thiamine thiazole synthaseAdd BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 205 | 2,3-didehydroalanine (Cys)1 Publication | 1 |
Post-translational modificationi
Proteomic databases
PaxDbi | P32318 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homooctamer; tetramer of dimers.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33392, 86 interactors |
DIPi | DIP-1703N |
IntActi | P32318, 5 interactors |
MINTi | P32318 |
STRINGi | 4932.YGR144W |
Miscellaneous databases
RNActi | P32318, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P32318 |
SMRi | P32318 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32318 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 97 – 98 | Substrate binding | 2 | |
Regioni | 301 – 303 | Substrate binding | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2960, Eukaryota |
HOGENOMi | CLU_053727_0_0_1 |
InParanoidi | P32318 |
OMAi | MWGGGMM |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_03158, THI4, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR027495, Sti35 IPR002922, Thi4_fam |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00292, TIGR00292, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSATSTATST SASQLHLNST PVTHCLSDIV KKEDWSDFKF APIRESTVSR
60 70 80 90 100
AMTSRYFKDL DKFAVSDVII VGAGSSGLSA AYVIAKNRPD LKVCIIESSV
110 120 130 140 150
APGGGSWLGG QLFSAMVMRK PAHLFLQELE IPYEDEGDYV VVKHAALFIS
160 170 180 190 200
TVLSKVLQLP NVKLFNATCV EDLVTRPPTE KGEVTVAGVV TNWTLVTQAH
210 220 230 240 250
GTQCCMDPNV IELAGYKNDG TRDLSQKHGV ILSTTGHDGP FGAFCAKRIV
260 270 280 290 300
DIDQNQKLGG MKGLDMNHAE HDVVIHSGAY AGVDNMYFAG MEVAELDGLN
310 320
RMGPTFGAMA LSGVHAAEQI LKHFAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61669 Genomic DNA Translation: CAA43843.1 Z72929 Genomic DNA Translation: CAA97157.1 Z72930 Genomic DNA Translation: CAA97159.1 X85807 Genomic DNA Translation: CAA59802.1 BK006941 Genomic DNA Translation: DAA08235.1 |
PIRi | S25321 |
RefSeqi | NP_011660.1, NM_001181273.1 |
Genome annotation databases
EnsemblFungii | YGR144W_mRNA; YGR144W; YGR144W |
GeneIDi | 853047 |
KEGGi | sce:YGR144W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61669 Genomic DNA Translation: CAA43843.1 Z72929 Genomic DNA Translation: CAA97157.1 Z72930 Genomic DNA Translation: CAA97159.1 X85807 Genomic DNA Translation: CAA59802.1 BK006941 Genomic DNA Translation: DAA08235.1 |
PIRi | S25321 |
RefSeqi | NP_011660.1, NM_001181273.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FPZ | X-ray | 1.82 | A/B | 1-326 | [»] | |
4Y4L | X-ray | 2.00 | A/B/C/D | 1-326 | [»] | |
AlphaFoldDBi | P32318 | |||||
SMRi | P32318 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33392, 86 interactors |
DIPi | DIP-1703N |
IntActi | P32318, 5 interactors |
MINTi | P32318 |
STRINGi | 4932.YGR144W |
Proteomic databases
PaxDbi | P32318 |
Genome annotation databases
EnsemblFungii | YGR144W_mRNA; YGR144W; YGR144W |
GeneIDi | 853047 |
KEGGi | sce:YGR144W |
Organism-specific databases
SGDi | S000003376, THI4 |
VEuPathDBi | FungiDB:YGR144W |
Phylogenomic databases
eggNOGi | KOG2960, Eukaryota |
HOGENOMi | CLU_053727_0_0_1 |
InParanoidi | P32318 |
OMAi | MWGGGMM |
Enzyme and pathway databases
BRENDAi | 2.4.2.60, 984 2.8.1.10, 984 |
Miscellaneous databases
EvolutionaryTracei | P32318 |
PROi | PR:P32318 |
RNActi | P32318, protein |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_03158, THI4, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR027495, Sti35 IPR002922, Thi4_fam |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00292, TIGR00292, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THI4_YEAST | |
Accessioni | P32318Primary (citable) accession number: P32318 Secondary accession number(s): D6VUS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families