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Protein

Delta-type opioid receptor

Gene

Oprd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.3 Publications

GO - Molecular functioni

  • enkephalin receptor activity Source: MGI
  • G protein-coupled receptor activity Source: GO_Central
  • neuropeptide binding Source: GO_Central
  • opioid receptor activity Source: MGI
  • receptor serine/threonine kinase binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-type opioid receptor
Short name:
D-OR-1
Short name:
DOR-1
Alternative name(s):
K56
MSL-2
Gene namesi
Name:Oprd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:97438 Oprd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47Extracellular1 PublicationAdd BLAST47
Transmembranei48 – 75Helical; Name=1Add BLAST28
Topological domaini76 – 85Cytoplasmic1 Publication10
Transmembranei86 – 110Helical; Name=2Add BLAST25
Topological domaini111 – 122Extracellular1 PublicationAdd BLAST12
Transmembranei123 – 144Helical; Name=3Add BLAST22
Topological domaini145 – 163Cytoplasmic1 PublicationAdd BLAST19
Transmembranei164 – 186Helical; Name=4Add BLAST23
Topological domaini187 – 206Extracellular1 PublicationAdd BLAST20
Transmembranei207 – 238Helical; Name=5Add BLAST32
Topological domaini239 – 261Cytoplasmic1 PublicationAdd BLAST23
Transmembranei262 – 284Helical; Name=6Add BLAST23
Topological domaini285 – 299Extracellular1 PublicationAdd BLAST15
Transmembranei300 – 321Helical; Name=7Add BLAST22
Topological domaini322 – 372Cytoplasmic1 PublicationAdd BLAST51

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate; they show no obvious phenotype and are fertile. Mutant mice show decreased analgesia in response to opioids, such as deltorphin-2. They do not develop analgesic tolerance to morphine.1 Publication

Chemistry databases

ChEMBLiCHEMBL3222
GuidetoPHARMACOLOGYi317

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699631 – 372Delta-type opioid receptorAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi18N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi121 ↔ 198PROSITE-ProRule annotation1 Publication
Lipidationi333S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Ubl conjugation

Proteomic databases

EPDiP32300
PaxDbiP32300
PRIDEiP32300

PTM databases

iPTMnetiP32300
PhosphoSitePlusiP32300

Expressioni

Tissue specificityi

Brain, with high concentrations in the basal ganglia and limbic regions.2 Publications

Gene expression databases

BgeeiENSMUSG00000050511 Expressed in 42 organ(s), highest expression level in Ammon's horn
CleanExiMM_OPRD1
ExpressionAtlasiP32300 baseline and differential
GenevisibleiP32300 MM

Interactioni

Subunit structurei

May form homooligomers. Forms a heterodimer with OPRM1 (PubMed:18836069). Interacts with GPRASP1 (By similarity). Interacts with RTP4; the interaction promotes cell surface localization of the OPRD1-OPRM1 heterodimer (PubMed:18836069).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Rtp4Q9ER802EBI-2615936,EBI-15731539

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46417N
IntActiP32300, 9 interactors
STRINGi10090.ENSMUSP00000050077

Chemistry databases

BindingDBiP32300

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32300
SMRiP32300
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230486
HOVERGENiHBG106919
InParanoidiP32300
KOiK04213
OMAiLCRAPCG
OrthoDBiEOG091G0HEN
PhylomeDBiP32300
TreeFamiTF315737

Family and domain databases

InterProiView protein in InterPro
IPR000321 Delta_opi_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001418 Opioid_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00525 DELTAOPIOIDR
PR00237 GPCRRHODOPSN
PR00384 OPIOIDR
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32300-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELVPSARAE LQSSPLVNLS DAFPSAFPSA GANASGSPGA RSASSLALAI
60 70 80 90 100
AITALYSAVC AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS
110 120 130 140 150
TLPFQSAKYL METWPFGELL CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV
160 170 180 190 200
CHPVKALDFR TPAKAKLINI CIWVLASGVG VPIMVMAVTQ PRDGAVVCML
210 220 230 240 250
QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL RSVRLLSGSK
260 270 280 290 300
EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL
310 320 330 340 350
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRTPCGRQ EPGSLRRPRQ
360 370
ATTRERVTAC TPSDGPGGGA AA
Length:372
Mass (Da):40,561
Last modified:October 1, 1993 - v1
Checksum:iBC3B3BBD2E52D3F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06322 mRNA Translation: AAA37522.1
L07271 mRNA No translation available.
L11064 mRNA Translation: AAA37520.1
S65335 mRNA Translation: AAA16009.1
S66181 mRNA Translation: AAB28546.1
CCDSiCCDS18718.1
PIRiB48227
RefSeqiNP_038650.3, NM_013622.3
UniGeneiMm.5243

Genome annotation databases

EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511
GeneIDi18386
KEGGimmu:18386
UCSCiuc008vap.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06322 mRNA Translation: AAA37522.1
L07271 mRNA No translation available.
L11064 mRNA Translation: AAA37520.1
S65335 mRNA Translation: AAA16009.1
S66181 mRNA Translation: AAB28546.1
CCDSiCCDS18718.1
PIRiB48227
RefSeqiNP_038650.3, NM_013622.3
UniGeneiMm.5243

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EJ4X-ray3.40A36-342[»]
ProteinModelPortaliP32300
SMRiP32300
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46417N
IntActiP32300, 9 interactors
STRINGi10090.ENSMUSP00000050077

Chemistry databases

BindingDBiP32300
ChEMBLiCHEMBL3222
GuidetoPHARMACOLOGYi317

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP32300
PhosphoSitePlusiP32300

Proteomic databases

EPDiP32300
PaxDbiP32300
PRIDEiP32300

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056336; ENSMUSP00000050077; ENSMUSG00000050511
GeneIDi18386
KEGGimmu:18386
UCSCiuc008vap.1 mouse

Organism-specific databases

CTDi4985
MGIiMGI:97438 Oprd1

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230486
HOVERGENiHBG106919
InParanoidiP32300
KOiK04213
OMAiLCRAPCG
OrthoDBiEOG091G0HEN
PhylomeDBiP32300
TreeFamiTF315737

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P32300
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050511 Expressed in 42 organ(s), highest expression level in Ammon's horn
CleanExiMM_OPRD1
ExpressionAtlasiP32300 baseline and differential
GenevisibleiP32300 MM

Family and domain databases

InterProiView protein in InterPro
IPR000321 Delta_opi_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001418 Opioid_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00525 DELTAOPIOIDR
PR00237 GPCRRHODOPSN
PR00384 OPIOIDR
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOPRD_MOUSE
AccessioniPrimary (citable) accession number: P32300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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Main funding by: National Institutes of Health

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