UniProtKB - P32299 (BKRB2_MOUSE)
Protein
B2 bradykinin receptor
Gene
Bdkrb2
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
GO - Molecular functioni
- beta-2 adrenergic receptor binding Source: MGI
- bradykinin receptor activity Source: MGI
- G protein-coupled receptor activity Source: GO_Central
- protease binding Source: MGI
- protein heterodimerization activity Source: MGI
- type 1 angiotensin receptor binding Source: MGI
GO - Biological processi
- acute inflammatory response to antigenic stimulus Source: MGI
- arachidonic acid secretion Source: MGI
- G protein-coupled receptor signaling pathway Source: GO_Central
- maintenance of blood-brain barrier Source: MGI
- negative regulation of blood pressure Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress Source: MGI
- negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator Source: MGI
- negative regulation of peptidyl-serine phosphorylation Source: MGI
- positive regulation of cytosolic calcium ion concentration Source: MGI
- response to salt stress Source: MGI
- smooth muscle contraction Source: InterPro
- vasoconstriction Source: InterPro
- vasodilation Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-375276, Peptide ligand-binding receptors R-MMU-416476, G alpha (q) signalling events R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: B2 bradykinin receptorShort name: B2R Short name: BK-2 receptor |
Gene namesi | Name:Bdkrb2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102845, Bdkrb2 |
Subcellular locationi
Plasma membrane
Endosome
- endosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 61 | ExtracellularSequence analysisAdd BLAST | 61 | |
Transmembranei | 62 – 85 | Helical; Name=1Sequence analysisAdd BLAST | 24 | |
Topological domaini | 86 – 94 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 95 – 119 | Helical; Name=2Sequence analysisAdd BLAST | 25 | |
Topological domaini | 120 – 132 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 133 – 154 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 155 – 176 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 177 – 199 | Helical; Name=4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 200 – 222 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 223 – 249 | Helical; Name=5Sequence analysisAdd BLAST | 27 | |
Topological domaini | 250 – 268 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 269 – 293 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 294 – 312 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 313 – 336 | Helical; Name=7Sequence analysisAdd BLAST | 24 | |
Topological domaini | 337 – 392 | CytoplasmicSequence analysisAdd BLAST | 56 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069191 | 1 – 392 | B2 bradykinin receptorAdd BLAST | 392 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 29 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 40 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 131 ↔ 212 | PROSITE-ProRule annotation | ||
Modified residuei | 157 | PhosphotyrosineBy similarity | 1 | |
Glycosylationi | 208 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 348 | PhosphotyrosineBy similarity | 1 | |
Lipidationi | 352 | S-palmitoyl cysteineSequence analysis | 1 | |
Modified residuei | 367 | PhosphoserineBy similarity | 1 | |
Modified residuei | 370 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 374 | Phosphoserine; by GRK6By similarity | 1 | |
Modified residuei | 376 | Phosphoserine; by GRK6By similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P32299 |
PRIDEi | P32299 |
PTM databases
GlyGeni | P32299, 3 sites |
iPTMneti | P32299 |
PhosphoSitePlusi | P32299 |
SwissPalmi | P32299 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021070, Expressed in esophagus and 50 other tissues |
Genevisiblei | P32299, MM |
Interactioni
Subunit structurei
Forms a complex with PECAM1 and GNAQ.
Interacts with PECAM1 (By similarity).
By similarityGO - Molecular functioni
- beta-2 adrenergic receptor binding Source: MGI
- protease binding Source: MGI
- protein heterodimerization activity Source: MGI
- type 1 angiotensin receptor binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000001652 |
Miscellaneous databases
RNActi | P32299, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTX6, Eukaryota |
GeneTreei | ENSGT01010000222307 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | P32299 |
OMAi | DICNQIF |
OrthoDBi | 826485at2759 |
PhylomeDBi | P32299 |
TreeFami | TF330024 |
Family and domain databases
InterProi | View protein in InterPro IPR001504, Brdyknn_2_rcpt IPR000496, Brdyknn_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00425, BRADYKININR PR00994, BRADYKINNB2R PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform Long (identifier: P32299-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPCSWKLLGF LSVHEPMPTA ASFGIEMFNV TTQVLGSALN GTLSKDNCPD
60 70 80 90 100
TEWWSWLNAI QAPFLWVLFL LAALENLFVL SVFFLHKNSC TVAEIYLGNL
110 120 130 140 150
AAADLILACG LPFWAITIAN NFDWVFGEVL CRVVNTMIYM NLYSSICFLM
160 170 180 190 200
LVSIDRYLAL VKTMSMGRMR GVRWAKLYSL VIWGCTLLLS SPMLVFRTMR
210 220 230 240 250
EYSEEGHNVT ACVIVYPSRS WEVFTNVLLN LVGFLLPLSV ITFCTVRILQ
260 270 280 290 300
VLRNNEMKKF KEVQTERKAT VLVLAVLGLF VLCWVPFQIS TFLDTLLRLG
310 320 330 340 350
VLSGCWDEHA VDVITQISSY VAYSNSGLNP LVYVIVGKRF RKKSREVYRV
360 370 380 390
LCQKGGCMGE PVQMENSMGT LRTSISVERQ IHKLQDWAGK KQ
Isoform Short (identifier: P32299-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-26: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 298 | R → A (PubMed:8394991).Curated | 1 | |
Sequence conflicti | 298 | R → A (PubMed:7959003).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001866 | 1 – 26 | Missing in isoform Short. 2 PublicationsAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69676 Genomic DNA Translation: CAA49357.1 X69682 mRNA Translation: CAA49362.1 L26047 Unassigned DNA Translation: AAA19797.1 X78438 mRNA Translation: CAA55202.1 L27595 Genomic DNA Translation: AAA62616.1 BC137755 mRNA Translation: AAI37756.1 |
CCDSi | CCDS49160.1 [P32299-1] |
PIRi | I49519 |
RefSeqi | NP_033877.3, NM_009747.2 [P32299-1] XP_006515505.1, XM_006515442.3 [P32299-1] |
Genome annotation databases
Ensembli | ENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070 [P32299-1] |
GeneIDi | 12062 |
KEGGi | mmu:12062 |
UCSCi | uc007oym.1, mouse [P32299-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69676 Genomic DNA Translation: CAA49357.1 X69682 mRNA Translation: CAA49362.1 L26047 Unassigned DNA Translation: AAA19797.1 X78438 mRNA Translation: CAA55202.1 L27595 Genomic DNA Translation: AAA62616.1 BC137755 mRNA Translation: AAI37756.1 |
CCDSi | CCDS49160.1 [P32299-1] |
PIRi | I49519 |
RefSeqi | NP_033877.3, NM_009747.2 [P32299-1] XP_006515505.1, XM_006515442.3 [P32299-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000001652 |
Chemistry databases
DrugCentrali | P32299 |
GuidetoPHARMACOLOGYi | 42 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P32299, 3 sites |
iPTMneti | P32299 |
PhosphoSitePlusi | P32299 |
SwissPalmi | P32299 |
Proteomic databases
PaxDbi | P32299 |
PRIDEi | P32299 |
Protocols and materials databases
Antibodypediai | 14184, 280 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000001652; ENSMUSP00000001652; ENSMUSG00000021070 [P32299-1] |
GeneIDi | 12062 |
KEGGi | mmu:12062 |
UCSCi | uc007oym.1, mouse [P32299-1] |
Organism-specific databases
CTDi | 624 |
MGIi | MGI:102845, Bdkrb2 |
Phylogenomic databases
eggNOGi | ENOG502QTX6, Eukaryota |
GeneTreei | ENSGT01010000222307 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | P32299 |
OMAi | DICNQIF |
OrthoDBi | 826485at2759 |
PhylomeDBi | P32299 |
TreeFami | TF330024 |
Enzyme and pathway databases
Reactomei | R-MMU-375276, Peptide ligand-binding receptors R-MMU-416476, G alpha (q) signalling events R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 12062, 0 hits in 17 CRISPR screens |
ChiTaRSi | Bdkrb2, mouse |
PROi | PR:P32299 |
RNActi | P32299, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021070, Expressed in esophagus and 50 other tissues |
Genevisiblei | P32299, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001504, Brdyknn_2_rcpt IPR000496, Brdyknn_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00425, BRADYKININR PR00994, BRADYKINNB2R PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BKRB2_MOUSE | |
Accessioni | P32299Primary (citable) accession number: P32299 Secondary accession number(s): B9EHE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | May 30, 2000 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries