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Protein

Dynamin-like GTPase MGM1, mitochondrial

Gene

MGM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane.4 Publications

Miscellaneous

Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi217 – 224GTPSequence analysis8
Nucleotide bindingi317 – 321GTPSequence analysis5
Nucleotide bindingi385 – 388GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

  • dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
  • membrane fusion Source: SGD
  • mitochondrial fission Source: GO_Central
  • mitochondrial fusion Source: UniProtKB
  • mitochondrial genome maintenance Source: UniProtKB
  • mitochondrial membrane organization Source: UniProtKB
  • mitochondrion inheritance Source: UniProtKB
  • mitochondrion organization Source: SGD
  • peroxisome fission Source: GO_Central

Keywordsi

Molecular functionHydrolase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33713-MONOMER
ReactomeiR-SCE-169911 Regulation of Apoptosis

Protein family/group databases

TCDBi9.B.25.1.1 the mitochondrial inner/outer membrane fusion (mmf) family

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-like GTPase MGM1, mitochondrial
Alternative name(s):
Mitochondrial division and morphology protein 17
Mitochondrial genome maintenance protein 1
Cleaved into the following 2 chains:
Gene namesi
Name:MGM1
Synonyms:MDM17
Ordered Locus Names:YOR211C
ORF Names:YOR50-1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR211C
SGDiS000005737 MGM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini60 – 72Mitochondrial matrixSequence analysisAdd BLAST13
Transmembranei73 – 92Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini93 – 881Mitochondrial intermembraneSequence analysisAdd BLAST789

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → A: Abolishes translation. 1
Mutagenesisi39M → A: No effect on translation. 1
Mutagenesisi79G → D or K: Loss of function; most MGM1 processed to s-MGM1. 1 Publication1
Mutagenesisi81M → A: No effect on translation. 1
Mutagenesisi92M → A: No effect on translation. 1
Mutagenesisi223K → A: Loss of stability. 1 Publication1
Mutagenesisi224S → A: Loss of GTPase activity. 2 Publications1
Mutagenesisi224S → N: Loss of GTPase activity. 2 Publications1
Mutagenesisi244T → A: Loss of GTPase activity. 1 Publication1
Mutagenesisi294E → K in mdm17; loss of function at 37 degrees Celsius. 1 Publication1
Mutagenesisi824R → A: Loss of GED function. 1 Publication1
Mutagenesisi854K → A: Loss of GED function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 59Mitochondrion1 PublicationAdd BLAST59
ChainiPRO_000000740060 – 881Dynamin-like GTPase MGM1 large isoformAdd BLAST822
ChainiPRO_0000007401140 – 881Dynamin-like GTPase MGM1 small isoformAdd BLAST742

Post-translational modificationi

Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.1 Publication

Proteomic databases

MaxQBiP32266
PaxDbiP32266
PRIDEiP32266

Miscellaneous databases

PMAP-CutDBiP32266

Interactioni

Subunit structurei

Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34606, 239 interactors
ComplexPortaliCPX-161 FZO1:MGM1:UGO1 complex
DIPiDIP-8008N
IntActiP32266, 8 interactors
MINTiP32266
STRINGi4932.YOR211C

Structurei

3D structure databases

ProteinModelPortaliP32266
SMRiP32266
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 505Dynamin-type GPROSITE-ProRule annotationAdd BLAST299
Domaini780 – 872GEDPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 224G1 motifPROSITE-ProRule annotation8
Regioni243 – 245G2 motifPROSITE-ProRule annotation3
Regioni317 – 320G3 motifPROSITE-ProRule annotation4
Regioni385 – 388G4 motifPROSITE-ProRule annotation4
Regioni414 – 417G5 motifPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 178Asp-rich (acidic)Add BLAST30

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074851
HOGENOMiHOG000206760
InParanoidiP32266
KOiK22140
OMAiKNKYYCP
OrthoDBiEOG092C0L57

Family and domain databases

CDDicd08771 DLP_1, 1 hit
InterProiView protein in InterPro
IPR001401 Dynamin_GTPase
IPR019762 Dynamin_GTPase_CS
IPR022812 Dynamin_SF
IPR030381 G_DYNAMIN_dom
IPR020850 GED_dom
IPR027417 P-loop_NTPase
PANTHERiPTHR11566 PTHR11566, 1 hit
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PRINTSiPR00195 DYNAMIN
SMARTiView protein in SMART
SM00053 DYNc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00410 G_DYNAMIN_1, 1 hit
PS51718 G_DYNAMIN_2, 1 hit
PS51388 GED, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32266-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA
60 70 80 90 100
LSFVITRRSI SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK
110 120 130 140 150
DKLDRIKDLG ESMKEKFNKM FSGDKSQDGG HGNDGTVPTA TLIAATSLDD
160 170 180 190 200
DESKRQGDPK DDDDEDDDDE DDENDSVDTT QDEMLNLTKQ MIEIRTILNK
210 220 230 240 250
VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS NMVTRRPIEL
260 270 280 290 300
TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP
310 320 330 340 350
IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI
360 370 380 390 400
ILAISAADVD LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN
410 420 430 440 450
NKKYPLSMGY VGVITKTPSS INRKHLGLFG EAPSSSLSGI FSKGQHGQSS
460 470 480 490 500
GEENTNGLKQ IVSHQFEKAY FKENKKYFTN CQVSTKKLRE KLIKILEISM
510 520 530 540 550
SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN IDVLKLGIKE
560 570 580 590 600
FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM
610 620 630 640 650
LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI
660 670 680 690 700
KDLVSNTAIN VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN
710 720 730 740 750
LIKEELRQCN SRYQAIKNAV GSKKLANVMG YLENESNLQK ETLGMSKLLL
760 770 780 790 800
ERGSEAIFLD KRCKVLSFRL KMLKNKCHST IEKDRCPEVF LSAVSDKLTS
810 820 830 840 850
TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE DPKISRHIEL
860 870 880
QKRKELLELA LEKIDSILVF KKSYKGVSKN L
Length:881
Mass (Da):99,178
Last modified:April 26, 2004 - v3
Checksum:i31524B40C5350096
GO

Sequence cautioni

The sequence CAA44637 differs from that shown. Reason: Frameshift at position 4.Curated
The sequence CAA63174 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99426 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99428 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → T in CAA44637 (PubMed:1532158).Curated1
Sequence conflicti129G → C in CAA44637 (PubMed:1532158).Curated1
Sequence conflicti170E → A in L07419 (PubMed:7916673).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62834 Genomic DNA Translation: CAA44637.1 Frameshift.
L07419 Genomic DNA No translation available.
Z75119 Genomic DNA Translation: CAA99426.1 Different initiation.
Z75120 Genomic DNA Translation: CAA99428.1 Different initiation.
X92441 Genomic DNA Translation: CAA63174.1 Different initiation.
L11274 Genomic DNA Translation: AAB59316.1
BK006948 Genomic DNA Translation: DAA10983.1
PIRiS33918
RefSeqiNP_014854.2, NM_001183630.1

Genome annotation databases

EnsemblFungiiYOR211C; YOR211C; YOR211C
GeneIDi854386
KEGGisce:YOR211C

Similar proteinsi

Entry informationi

Entry nameiMGM1_YEAST
AccessioniPrimary (citable) accession number: P32266
Secondary accession number(s): D6W2R7, Q02609, Q08627
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 26, 2004
Last modified: July 18, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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