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UniProtKB - P32266 (MGM1_YEAST)
Protein
Dynamin-like GTPase MGM1, mitochondrial
Gene
MGM1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Dynamin-related GTPase required for mitochondrial fusion. Coordinates interaction between the inner and outer mitochondrial membrane to promote the formation of the double membrane.
4 PublicationsMiscellaneous
Deletion of MGM1 causes the mitochondria to fragment and aggregate, and subsequently to lose their mitochondrial DNA and become respiration deficient.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 217 – 224 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 317 – 321 | GTPSequence analysis | 5 | |
Nucleotide bindingi | 385 – 388 | GTPSequence analysis | 4 |
GO - Molecular functioni
- cardiolipin binding Source: SGD
- GTPase activity Source: SGD
- GTP binding Source: UniProtKB-KW
- microtubule binding Source: GO_Central
- phosphatidic acid binding Source: SGD
- phosphatidylinositol-3,5-bisphosphate binding Source: SGD
- phosphatidylserine binding Source: SGD
GO - Biological processi
- heme transport Source: SGD
- membrane fusion Source: SGD
- mitochondrial fusion Source: UniProtKB
- mitochondrial genome maintenance Source: UniProtKB
- mitochondrial inner membrane fusion Source: ComplexPortal
- mitochondrial membrane organization Source: UniProtKB
- mitochondrial outer membrane fusion Source: ComplexPortal
- mitochondrion inheritance Source: UniProtKB
- mitochondrion organization Source: SGD
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-169911, Regulation of Apoptosis R-SCE-196025, Formation of annular gap junctions |
Protein family/group databases
TCDBi | 1.N.6.1.1, the mitochondrial inner/outer membrane fusion (mmf) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dynamin-like GTPase MGM1, mitochondrialAlternative name(s): Mitochondrial division and morphology protein 17 Mitochondrial genome maintenance protein 1 Cleaved into the following 2 chains: |
Gene namesi | Name:MGM1 Synonyms:MDM17 Ordered Locus Names:YOR211C ORF Names:YOR50-1 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005737, MGM1 |
VEuPathDBi | FungiDB:YOR211C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Single-pass type II membrane protein Sequence analysis; Intermembrane side 1 Publication
- Mitochondrion intermembrane space 1 Publication
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side
Cytoskeleton
- microtubule Source: GO_Central
Mitochondrion
- extrinsic component of mitochondrial inner membrane Source: SGD
- intrinsic component of mitochondrial inner membrane Source: SGD
- mitochondrial crista Source: SGD
- mitochondrial inner boundary membrane Source: SGD
- mitochondrial intermembrane space Source: UniProtKB
- mitochondrion Source: ComplexPortal
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 60 – 72 | Mitochondrial matrixSequence analysisAdd BLAST | 13 | |
Transmembranei | 73 – 92 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 93 – 881 | Mitochondrial intermembraneSequence analysisAdd BLAST | 789 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 | M → A: Abolishes translation. | 1 | |
Mutagenesisi | 39 | M → A: No effect on translation. | 1 | |
Mutagenesisi | 79 | G → D or K: Loss of function; most MGM1 processed to s-MGM1. 1 Publication | 1 | |
Mutagenesisi | 81 | M → A: No effect on translation. | 1 | |
Mutagenesisi | 92 | M → A: No effect on translation. | 1 | |
Mutagenesisi | 223 | K → A: Loss of stability. 1 Publication | 1 | |
Mutagenesisi | 224 | S → A: Loss of GTPase activity. 2 Publications | 1 | |
Mutagenesisi | 224 | S → N: Loss of GTPase activity. 2 Publications | 1 | |
Mutagenesisi | 244 | T → A: Loss of GTPase activity. 1 Publication | 1 | |
Mutagenesisi | 294 | E → K in mdm17; loss of function at 37 degrees Celsius. 1 Publication | 1 | |
Mutagenesisi | 824 | R → A: Loss of GED function. 1 Publication | 1 | |
Mutagenesisi | 854 | K → A: Loss of GED function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 59 | Mitochondrion1 PublicationAdd BLAST | 59 | |
ChainiPRO_0000007400 | 60 – 881 | Dynamin-like GTPase MGM1 large isoformAdd BLAST | 822 | |
ChainiPRO_0000007401 | 140 – 881 | Dynamin-like GTPase MGM1 small isoformAdd BLAST | 742 |
Post-translational modificationi
Cleavage of the transit peptide by mitochondrial processing protease (MPP) produces a long integral membrane form of MGM1 (l-MGM1). Further processing by the rhomboid protease PCP1 produces a short peripheral membrane form of MGM1 (s-MGM1). Both isoforms are required for full activity.1 Publication
Proteomic databases
MaxQBi | P32266 |
PaxDbi | P32266 |
PRIDEi | P32266 |
PTM databases
iPTMneti | P32266 |
Interactioni
Subunit structurei
Homooligomer. Associates with FZO1 through interaction with the intermembrane space domain of UGO1 which binds FZO1 through its cytoplasmic domain.
3 PublicationsBinary interactionsi
GO - Molecular functioni
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 34606, 240 interactors |
ComplexPortali | CPX-161, FZO1-MGM1-UGO1 complex |
DIPi | DIP-8008N |
IntActi | P32266, 6 interactors |
MINTi | P32266 |
STRINGi | 4932.YOR211C |
Miscellaneous databases
RNActi | P32266, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32266 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 207 – 505 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 299 | |
Domaini | 780 – 872 | GEDPROSITE-ProRule annotationAdd BLAST | 93 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 145 – 183 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 217 – 224 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 243 – 245 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 317 – 320 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 385 – 388 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 414 – 417 | G5 motifPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 161 – 179 | Acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation
Keywords - Domaini
Signal-anchor, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00550000074851 |
HOGENOMi | CLU_008640_0_1_1 |
InParanoidi | P32266 |
OMAi | GYIQVAG |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR022812, Dynamin IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR045063, Dynamin_N IPR030381, G_DYNAMIN_dom IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32266-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNASPVRLLI LRRQLATHPA ILYSSPYIKS PLVHLHSRMS NVHRSAHANA
60 70 80 90 100
LSFVITRRSI SHFPKIISKI IRLPIYVGGG MAAAGSYIAY KMEEASSFTK
110 120 130 140 150
DKLDRIKDLG ESMKEKFNKM FSGDKSQDGG HGNDGTVPTA TLIAATSLDD
160 170 180 190 200
DESKRQGDPK DDDDEDDDDE DDENDSVDTT QDEMLNLTKQ MIEIRTILNK
210 220 230 240 250
VDSSSAHLTL PSIVVIGSQS SGKSSVLESI VGREFLPKGS NMVTRRPIEL
260 270 280 290 300
TLVNTPNSNN VTADFPSMRL YNIKDFKEVK RMLMELNMAV PTSEAVSEEP
310 320 330 340 350
IQLTIKSSRV PDLSLVDLPG YIQVEAADQP IELKTKIRDL CEKYLTAPNI
360 370 380 390 400
ILAISAADVD LANSSALKAS KAADPKGLRT IGVITKLDLV DPEKARSILN
410 420 430 440 450
NKKYPLSMGY VGVITKTPSS INRKHLGLFG EAPSSSLSGI FSKGQHGQSS
460 470 480 490 500
GEENTNGLKQ IVSHQFEKAY FKENKKYFTN CQVSTKKLRE KLIKILEISM
510 520 530 540 550
SNALEPTSTL IQQELDDTSY LFKVEFNDRH LTPKSYLLNN IDVLKLGIKE
560 570 580 590 600
FQEKFHRNEL KSILRAELDQ KVLDVLATRY WKDDNLQDLS SSKLESDTDM
610 620 630 640 650
LYWHKKLELA SSGLTKMGIG RLSTMLTTNA ILKELDNILE STQLKNHELI
660 670 680 690 700
KDLVSNTAIN VLNSKYYSTA DQVENCIKPF KYEIDLEERD WSLARQHSIN
710 720 730 740 750
LIKEELRQCN SRYQAIKNAV GSKKLANVMG YLENESNLQK ETLGMSKLLL
760 770 780 790 800
ERGSEAIFLD KRCKVLSFRL KMLKNKCHST IEKDRCPEVF LSAVSDKLTS
810 820 830 840 850
TAVLFLNVEL LSDFFYNFPI ELDRRLTLLG DEQVEMFAKE DPKISRHIEL
860 870 880
QKRKELLELA LEKIDSILVF KKSYKGVSKN L
Sequence cautioni
The sequence CAA44637 differs from that shown. Reason: Frameshift.Curated
The sequence CAA63174 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99426 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA99428 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | S → T in CAA44637 (PubMed:1532158).Curated | 1 | |
Sequence conflicti | 129 | G → C in CAA44637 (PubMed:1532158).Curated | 1 | |
Sequence conflicti | 170 | E → A in L07419 (PubMed:7916673).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62834 Genomic DNA Translation: CAA44637.1 Frameshift. L07419 Genomic DNA No translation available. Z75119 Genomic DNA Translation: CAA99426.1 Different initiation. Z75120 Genomic DNA Translation: CAA99428.1 Different initiation. X92441 Genomic DNA Translation: CAA63174.1 Different initiation. L11274 Genomic DNA Translation: AAB59316.1 BK006948 Genomic DNA Translation: DAA10983.1 |
PIRi | S33918 |
RefSeqi | NP_014854.2, NM_001183630.1 |
Genome annotation databases
EnsemblFungii | YOR211C_mRNA; YOR211C; YOR211C |
GeneIDi | 854386 |
KEGGi | sce:YOR211C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X62834 Genomic DNA Translation: CAA44637.1 Frameshift. L07419 Genomic DNA No translation available. Z75119 Genomic DNA Translation: CAA99426.1 Different initiation. Z75120 Genomic DNA Translation: CAA99428.1 Different initiation. X92441 Genomic DNA Translation: CAA63174.1 Different initiation. L11274 Genomic DNA Translation: AAB59316.1 BK006948 Genomic DNA Translation: DAA10983.1 |
PIRi | S33918 |
RefSeqi | NP_014854.2, NM_001183630.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JSJ | X-ray | 3.20 | A/B/C | 184-881 | [»] | |
SMRi | P32266 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34606, 240 interactors |
ComplexPortali | CPX-161, FZO1-MGM1-UGO1 complex |
DIPi | DIP-8008N |
IntActi | P32266, 6 interactors |
MINTi | P32266 |
STRINGi | 4932.YOR211C |
Protein family/group databases
TCDBi | 1.N.6.1.1, the mitochondrial inner/outer membrane fusion (mmf) family |
PTM databases
iPTMneti | P32266 |
Proteomic databases
MaxQBi | P32266 |
PaxDbi | P32266 |
PRIDEi | P32266 |
Genome annotation databases
EnsemblFungii | YOR211C_mRNA; YOR211C; YOR211C |
GeneIDi | 854386 |
KEGGi | sce:YOR211C |
Organism-specific databases
SGDi | S000005737, MGM1 |
VEuPathDBi | FungiDB:YOR211C |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00550000074851 |
HOGENOMi | CLU_008640_0_1_1 |
InParanoidi | P32266 |
OMAi | GYIQVAG |
Enzyme and pathway databases
Reactomei | R-SCE-169911, Regulation of Apoptosis R-SCE-196025, Formation of annular gap junctions |
Miscellaneous databases
PROi | PR:P32266 |
RNActi | P32266, protein |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR022812, Dynamin IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR045063, Dynamin_N IPR030381, G_DYNAMIN_dom IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MGM1_YEAST | |
Accessioni | P32266Primary (citable) accession number: P32266 Secondary accession number(s): D6W2R7, Q02609, Q08627 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | April 26, 2004 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families