UniProtKB - P32263 (P5CR_YEAST)
Protein
Pyrroline-5-carboxylate reductase
Gene
PRO3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 43500 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
: L-proline biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.Proteins known to be involved in this subpathway in this organism are:
- Pyrroline-5-carboxylate reductase (PRO3)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- identical protein binding Source: IntAct
- pyrroline-5-carboxylate reductase activity Source: SGD
GO - Biological processi
- L-proline biosynthetic process Source: GO_Central
- proline biosynthetic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Proline biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:YER023W-MONOMER |
Reactomei | R-SCE-8964539, Glutamate and glutamine metabolism |
UniPathwayi | UPA00098;UER00361 |
Names & Taxonomyi
Protein namesi | Recommended name: Pyrroline-5-carboxylate reductase (EC:1.5.1.2)Short name: P5C reductase Short name: P5CR |
Gene namesi | Name:PRO3 Synonyms:ORE2 Ordered Locus Names:YER023W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YER023W |
SGDi | S000000825, PRO3 |
Subcellular locationi
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187328 | 1 – 286 | Pyrroline-5-carboxylate reductaseAdd BLAST | 286 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 171 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 246 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 279 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P32263 |
PaxDbi | P32263 |
PRIDEi | P32263 |
PTM databases
iPTMneti | P32263 |
Interactioni
Subunit structurei
Homotetramer.
Binary interactionsi
P32263
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-13885,EBI-13885 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 36757, 41 interactors |
DIPi | DIP-1529N |
IntActi | P32263, 11 interactors |
MINTi | P32263 |
STRINGi | 4932.YER023W |
Miscellaneous databases
RNActi | P32263, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the pyrroline-5-carboxylate reductase family.Curated
Phylogenomic databases
eggNOGi | KOG3124, Eukaryota |
GeneTreei | ENSGT00950000183044 |
HOGENOMi | CLU_042344_1_2_1 |
InParanoidi | P32263 |
OMAi | VVRVMTN |
Family and domain databases
HAMAPi | MF_01925, P5C_reductase, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N IPR029036, P5CR_dimer IPR000304, Pyrroline-COOH_reductase |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF14748, P5CR_dimer, 1 hit |
PIRSFi | PIRSF000193, Pyrrol-5-carb_rd, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00112, proC, 1 hit |
PROSITEi | View protein in PROSITE PS00521, P5CR, 1 hit |
i Sequence
Sequence statusi: Complete.
P32263-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTYTLAILGC GVMGQALLSA IYNAPKAADE TAAAFYPSKI ITCNHDEPSA
60 70 80 90 100
QQVTDLVETF DESPNGIKVE STYGHNVSAV EEASVVLLGT KPFLAEEVLN
110 120 130 140 150
GVKSVIGGKL LISLAAGWTI DQLSQYTSTV CRVMTNTPAK YGYGCAVVSY
160 170 180 190 200
SADVSKEQKP LVNELISQVG KYVELPEKNM DAATALVGSG PAFVLLMLES
210 220 230 240 250
LMESGLKLGI PLQESKECAM KVLEGTVKMV EKSGAHPSVL KHQVCTPGGT
260 270 280
TIAGLCVMEE KGVKSGIING VEEAARVASQ LGQKKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57886 Genomic DNA Translation: AAA34905.1 X57338 Genomic DNA Translation: CAA40614.1 U18778 Genomic DNA Translation: AAB64556.1 BK006939 Genomic DNA Translation: DAA07676.1 |
PIRi | S25293 |
RefSeqi | NP_010940.3, NM_001178914.3 |
Genome annotation databases
EnsemblFungii | YER023W_mRNA; YER023W; YER023W |
GeneIDi | 856744 |
KEGGi | sce:YER023W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57886 Genomic DNA Translation: AAA34905.1 X57338 Genomic DNA Translation: CAA40614.1 U18778 Genomic DNA Translation: AAB64556.1 BK006939 Genomic DNA Translation: DAA07676.1 |
PIRi | S25293 |
RefSeqi | NP_010940.3, NM_001178914.3 |
3D structure databases
SMRi | P32263 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36757, 41 interactors |
DIPi | DIP-1529N |
IntActi | P32263, 11 interactors |
MINTi | P32263 |
STRINGi | 4932.YER023W |
PTM databases
iPTMneti | P32263 |
Proteomic databases
MaxQBi | P32263 |
PaxDbi | P32263 |
PRIDEi | P32263 |
Genome annotation databases
EnsemblFungii | YER023W_mRNA; YER023W; YER023W |
GeneIDi | 856744 |
KEGGi | sce:YER023W |
Organism-specific databases
EuPathDBi | FungiDB:YER023W |
SGDi | S000000825, PRO3 |
Phylogenomic databases
eggNOGi | KOG3124, Eukaryota |
GeneTreei | ENSGT00950000183044 |
HOGENOMi | CLU_042344_1_2_1 |
InParanoidi | P32263 |
OMAi | VVRVMTN |
Enzyme and pathway databases
UniPathwayi | UPA00098;UER00361 |
BioCyci | MetaCyc:YER023W-MONOMER |
Reactomei | R-SCE-8964539, Glutamate and glutamine metabolism |
Miscellaneous databases
PROi | PR:P32263 |
RNActi | P32263, protein |
Family and domain databases
HAMAPi | MF_01925, P5C_reductase, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N IPR029036, P5CR_dimer IPR000304, Pyrroline-COOH_reductase |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF14748, P5CR_dimer, 1 hit |
PIRSFi | PIRSF000193, Pyrrol-5-carb_rd, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00112, proC, 1 hit |
PROSITEi | View protein in PROSITE PS00521, P5CR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P5CR_YEAST | |
Accessioni | P32263Primary (citable) accession number: P32263 Secondary accession number(s): D3DLS2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD