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UniProtKB - P32240 (PE2R4_MOUSE)
Protein
Prostaglandin E2 receptor EP4 subtype
Gene
Ptger4
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Caution
Was originally designated as the EP2 subtype.Curated
GO - Molecular functioni
- prostaglandin E receptor activity Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
- bone development Source: UniProtKB
- cellular response to mechanical stimulus Source: UniProtKB
- cellular response to prostaglandin E stimulus Source: GO_Central
- chemokinesis Source: MGI
- ductus arteriosus closure Source: MGI
- ERK1 and ERK2 cascade Source: UniProtKB
- inflammatory response Source: GO_Central
- JNK cascade Source: UniProtKB
- negative regulation of circadian sleep/wake cycle, REM sleep Source: MGI
- negative regulation of cytokine production Source: UniProtKB
- negative regulation of ductus arteriosus closure Source: MGI
- negative regulation of endothelin production Source: MGI
- negative regulation of eosinophil extravasation Source: UniProtKB
- negative regulation of hydrogen peroxide metabolic process Source: MGI
- negative regulation of inflammatory response Source: MGI
- negative regulation of integrin activation Source: UniProtKB
- negative regulation of interleukin-1 alpha production Source: MGI
- negative regulation of nitric-oxide synthase biosynthetic process Source: MGI
- negative regulation of peptide secretion Source: MGI
- negative regulation of small intestine smooth muscle contraction Source: MGI
- negative regulation of tumor necrosis factor production Source: MGI
- positive regulation of antral ovarian follicle growth Source: MGI
- positive regulation of bone resorption Source: MGI
- positive regulation of calcitonin secretion Source: MGI
- positive regulation of cAMP-mediated signaling Source: MGI
- positive regulation of cell adhesion Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of chemokinesis Source: MGI
- positive regulation of circadian sleep/wake cycle, non-REM sleep Source: MGI
- positive regulation of cytokine production Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- positive regulation of gene expression Source: MGI
- positive regulation of hyaluronan biosynthetic process Source: MGI
- positive regulation of inflammatory response Source: UniProtKB
- positive regulation of interleukin-10 production Source: MGI
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of matrix metallopeptidase secretion Source: MGI
- positive regulation of mucus secretion Source: MGI
- positive regulation of neutrophil extravasation Source: MGI
- positive regulation of ossification Source: MGI
- positive regulation of osteoblast differentiation Source: MGI
- positive regulation of ovarian follicle development Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of protein secretion Source: MGI
- positive regulation of renal sodium excretion Source: MGI
- positive regulation of smooth muscle cell migration Source: MGI
- positive regulation of substance P secretion Source: MGI
- positive regulation of substance P secretion, neurotransmission Source: MGI
- positive regulation of tyrosine phosphorylation of STAT protein Source: MGI
- positive regulation of urine volume Source: MGI
- positive regulation of wound healing Source: MGI
- regulation of circadian sleep/wake cycle, wakefulness Source: MGI
- regulation of ossification Source: MGI
- regulation of stress fiber assembly Source: UniProtKB
- response to lipopolysaccharide Source: MGI
- T-helper cell differentiation Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-391908, Prostanoid ligand receptors R-MMU-418555, G alpha (s) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Prostaglandin E2 receptor EP4 subtypeShort name: PGE receptor EP4 subtype Short name: PGE2 receptor EP4 subtype Alternative name(s): Prostanoid EP4 receptor |
Gene namesi | Name:Ptger4 Synonyms:Ptgerep4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104311, Ptger4 |
VEuPathDBi | HostDB:ENSMUSG00000039942 |
Subcellular locationi
Plasma membrane
Nucleus
- nuclear membrane Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- neuron projection terminus Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 44 | ExtracellularSequence analysisAdd BLAST | 44 | |
Transmembranei | 45 – 68 | Helical; Name=1Sequence analysisAdd BLAST | 24 | |
Topological domaini | 69 – 80 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 81 – 104 | Helical; Name=2Sequence analysisAdd BLAST | 24 | |
Topological domaini | 105 – 121 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 122 – 140 | Helical; Name=3Sequence analysisAdd BLAST | 19 | |
Topological domaini | 141 – 160 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 161 – 185 | Helical; Name=4Sequence analysisAdd BLAST | 25 | |
Topological domaini | 186 – 209 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 210 – 236 | Helical; Name=5Sequence analysisAdd BLAST | 27 | |
Topological domaini | 237 – 295 | CytoplasmicSequence analysisAdd BLAST | 59 | |
Transmembranei | 296 – 323 | Helical; Name=6Sequence analysisAdd BLAST | 28 | |
Topological domaini | 324 – 340 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 341 – 360 | Helical; Name=7Sequence analysisAdd BLAST | 20 | |
Topological domaini | 361 – 513 | CytoplasmicSequence analysisAdd BLAST | 153 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL2489 |
DrugCentrali | P32240 |
GuidetoPHARMACOLOGYi | 343 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070065 | 1 – 513 | Prostaglandin E2 receptor EP4 subtypeAdd BLAST | 513 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 117 ↔ 195 | PROSITE-ProRule annotation | ||
Modified residuei | 402 | PhosphoserineBy similarity | 1 | |
Modified residuei | 405 | PhosphoserineBy similarity | 1 | |
Modified residuei | 407 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P32240 |
PeptideAtlasi | P32240 |
PRIDEi | P32240 |
ProteomicsDBi | 287665 |
PTM databases
GlyGeni | P32240, 1 site |
iPTMneti | P32240 |
PhosphoSitePlusi | P32240 |
Expressioni
Tissue specificityi
Abundant expression in ileum, thymus and mastocytoma P-815 cells. Also observed in lung, spleen, heart and uterus.
Gene expression databases
Bgeei | ENSMUSG00000039942, Expressed in renal corpuscle and 251 other tissues |
ExpressionAtlasi | P32240, baseline and differential |
Genevisiblei | P32240, MM |
Interactioni
Subunit structurei
Interacts with FEM1A.
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000048736 |
Chemistry databases
BindingDBi | P32240 |
Miscellaneous databases
RNActi | P32240, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 383 – 403 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 385 – 403 | Polar residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01030000234559 |
InParanoidi | P32240 |
OMAi | LLYMPDL |
OrthoDBi | 972015at2759 |
PhylomeDBi | P32240 |
TreeFami | TF324982 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001758, Prost_EP4_rcpt IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF6, PTHR11866:SF6, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00586, PRSTNOIDEP4R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P32240-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEVGGTIPR SNRELQRCVL LTTTIMSIPG VNASFSSTPE RLNSPVTIPA
60 70 80 90 100
VMFIFGVVGN LVAIVVLCKS RKEQKETTFY TLVCGLAVTD LLGTLLVSPV
110 120 130 140 150
TIATYMKGQW PGDQALCDYS TFILLFFGLS GLSIICAMSI ERYLAINHAY
160 170 180 190 200
FYSHYVDKRL AGLTLFAIYA SNVLFCALPN MGLGRSERQY PGTWCFIDWT
210 220 230 240 250
TNVTAYAAFS YMYAGFSSFL ILATVLCNVL VCGALLRMHR QFMRRTSLGT
260 270 280 290 300
EQHHAAAAAA VASVACRGHA GASPALQRLS DFRRRRSFRR IAGAEIQMVI
310 320 330 340 350
LLIATSLVVL ICSIPLVVRV FINQLYQPNV VKDISRNPDL QAIRIASVNP
360 370 380 390 400
ILDPWIYILL RKTVLSKAIE KIKCLFCRIG GSGRDSSAQH CSESRRTSSA
410 420 430 440 450
MSGHSRSFLA RELKEISSTS QTLLYLPDLT ESSLGGRNLL PGSHGMGLTQ
460 470 480 490 500
ADTTSLRTLR ISETSDSSQG QDSESVLLVD EVSGSHREEP ASKGNSLQVT
510
FPSETLKLSE KCI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ91VE4 | Q91VE4_MOUSE | Prostaglandin E2 receptor EP4 subty... | Ptger4 | 488 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13458 mRNA Translation: BAA02714.1 U70365 Genomic DNA Translation: AAC52897.1 |
CCDSi | CCDS27365.1 |
PIRi | A46638 |
RefSeqi | NP_032991.1, NM_008965.2 |
Genome annotation databases
Ensembli | ENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942 |
GeneIDi | 19219 |
KEGGi | mmu:19219 |
UCSCi | uc007vcy.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13458 mRNA Translation: BAA02714.1 U70365 Genomic DNA Translation: AAC52897.1 |
CCDSi | CCDS27365.1 |
PIRi | A46638 |
RefSeqi | NP_032991.1, NM_008965.2 |
3D structure databases
SMRi | P32240 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000048736 |
Chemistry databases
BindingDBi | P32240 |
ChEMBLi | CHEMBL2489 |
DrugCentrali | P32240 |
GuidetoPHARMACOLOGYi | 343 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P32240, 1 site |
iPTMneti | P32240 |
PhosphoSitePlusi | P32240 |
Proteomic databases
PaxDbi | P32240 |
PeptideAtlasi | P32240 |
PRIDEi | P32240 |
ProteomicsDBi | 287665 |
Protocols and materials databases
Antibodypediai | 2762, 375 antibodies from 36 providers |
DNASUi | 19219 |
Genome annotation databases
Ensembli | ENSMUST00000047379; ENSMUSP00000048736; ENSMUSG00000039942 |
GeneIDi | 19219 |
KEGGi | mmu:19219 |
UCSCi | uc007vcy.3, mouse |
Organism-specific databases
CTDi | 5734 |
MGIi | MGI:104311, Ptger4 |
VEuPathDBi | HostDB:ENSMUSG00000039942 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01030000234559 |
InParanoidi | P32240 |
OMAi | LLYMPDL |
OrthoDBi | 972015at2759 |
PhylomeDBi | P32240 |
TreeFami | TF324982 |
Enzyme and pathway databases
Reactomei | R-MMU-391908, Prostanoid ligand receptors R-MMU-418555, G alpha (s) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 19219, 3 hits in 64 CRISPR screens |
ChiTaRSi | Ptger4, mouse |
PROi | PR:P32240 |
RNActi | P32240, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039942, Expressed in renal corpuscle and 251 other tissues |
ExpressionAtlasi | P32240, baseline and differential |
Genevisiblei | P32240, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001758, Prost_EP4_rcpt IPR008365, Prostanoid_rcpt IPR001244, Prostglndn_DP_rcpt |
PANTHERi | PTHR11866, PTHR11866, 1 hit PTHR11866:SF6, PTHR11866:SF6, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00428, PROSTAGLNDNR PR01788, PROSTANOIDR PR00586, PRSTNOIDEP4R |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PE2R4_MOUSE | |
Accessioni | P32240Primary (citable) accession number: P32240 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families