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Protein

Muscarinic acetylcholine receptor M4

Gene

Chrm4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390648 Muscarinic acetylcholine receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Alternative name(s):
Mm4 mAChR
Gene namesi
Name:Chrm4
Synonyms:Chrm-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88399 Chrm4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30ExtracellularBy similarityAdd BLAST30
Transmembranei31 – 53Helical; Name=1By similarityAdd BLAST23
Topological domaini54 – 67CytoplasmicBy similarityAdd BLAST14
Transmembranei68 – 88Helical; Name=2By similarityAdd BLAST21
Topological domaini89 – 105ExtracellularBy similarityAdd BLAST17
Transmembranei106 – 127Helical; Name=3By similarityAdd BLAST22
Topological domaini128 – 147CytoplasmicBy similarityAdd BLAST20
Transmembranei148 – 170Helical; Name=4By similarityAdd BLAST23
Topological domaini171 – 192ExtracellularBy similarityAdd BLAST22
Transmembranei193 – 215Helical; Name=5By similarityAdd BLAST23
Topological domaini216 – 401CytoplasmicBy similarityAdd BLAST186
Transmembranei402 – 422Helical; Name=6By similarityAdd BLAST21
Topological domaini423 – 436ExtracellularBy similarityAdd BLAST14
Transmembranei437 – 456Helical; Name=7By similarityAdd BLAST20
Topological domaini457 – 479CytoplasmicBy similarityAdd BLAST23

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4572

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690381 – 479Muscarinic acetylcholine receptor M4Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi13N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi104 ↔ 184PROSITE-ProRule annotation
Modified residuei459PhosphothreonineSequence analysis1
Modified residuei463PhosphothreonineSequence analysis1
Modified residuei477PhosphothreonineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32211
PRIDEiP32211

PTM databases

iPTMnetiP32211
PhosphoSitePlusiP32211
SwissPalmiP32211

Expressioni

Gene expression databases

BgeeiENSMUSG00000040495 Expressed in 45 organ(s), highest expression level in retina
CleanExiMM_CHRM4
ExpressionAtlasiP32211 baseline and differential
GenevisibleiP32211 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP32211, 1 interactor
MINTiP32211
STRINGi10090.ENSMUSP00000040808

Chemistry databases

BindingDBiP32211

Structurei

3D structure databases

ProteinModelPortaliP32211
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP32211
KOiK04132
OMAiTSTHNRY
OrthoDBiEOG091G06VI
PhylomeDBiP32211
TreeFamiTF320495

Family and domain databases

CDDicd15298 7tmA_mAChR_M4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001432 Musac_Ach_M4_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF6 PTHR43914:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00541 MUSCRINICM4R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32211-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI
60 70 80 90 100
LVMLSIKVNR QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG
110 120 130 140 150
AVVCDLWLAL DYVVSNASVM NLLIISFDRY FCVTKPLTYP ARRTTKMAGL
160 170 180 190 200
MIAAAWVLSF VLWAPAILFW QFVVGKRTVP DNQCFIQFLS NPAVTFGTAI
210 220 230 240 250
AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT LAFLKSPLMK
260 270 280 290 300
PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA
310 320 330 340 350
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG
360 370 380 390 400
SECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PERVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):52,973
Last modified:October 1, 1993 - v1
Checksum:i04F301E78814CD70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322 – 323AL → V in AAB33577 (PubMed:7874308).Curated2
Sequence conflicti351S → N in AAB33577 (PubMed:7874308).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA Translation: CAA45071.1
S74916 mRNA Translation: AAB33577.1
CCDSiCCDS16439.1
PIRiS33776
RefSeqiNP_031725.1, NM_007699.2
UniGeneiMm.330405

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495
GeneIDi12672
KEGGimmu:12672
UCSCiuc008kwv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63473 Genomic DNA Translation: CAA45071.1
S74916 mRNA Translation: AAB33577.1
CCDSiCCDS16439.1
PIRiS33776
RefSeqiNP_031725.1, NM_007699.2
UniGeneiMm.330405

3D structure databases

ProteinModelPortaliP32211
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32211, 1 interactor
MINTiP32211
STRINGi10090.ENSMUSP00000040808

Chemistry databases

BindingDBiP32211
ChEMBLiCHEMBL4572

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP32211
PhosphoSitePlusiP32211
SwissPalmiP32211

Proteomic databases

PaxDbiP32211
PRIDEiP32211

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495
GeneIDi12672
KEGGimmu:12672
UCSCiuc008kwv.2 mouse

Organism-specific databases

CTDi1132
MGIiMGI:88399 Chrm4

Phylogenomic databases

eggNOGiKOG4220 Eukaryota
ENOG410YCQR LUCA
GeneTreeiENSGT00780000121874
HOGENOMiHOG000231484
HOVERGENiHBG105720
InParanoidiP32211
KOiK04132
OMAiTSTHNRY
OrthoDBiEOG091G06VI
PhylomeDBiP32211
TreeFamiTF320495

Enzyme and pathway databases

ReactomeiR-MMU-390648 Muscarinic acetylcholine receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P32211
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040495 Expressed in 45 organ(s), highest expression level in retina
CleanExiMM_CHRM4
ExpressionAtlasiP32211 baseline and differential
GenevisibleiP32211 MM

Family and domain databases

CDDicd15298 7tmA_mAChR_M4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001432 Musac_Ach_M4_rcpt
IPR000995 Musac_Ach_rcpt
PANTHERiPTHR43914:SF6 PTHR43914:SF6, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00243 MUSCARINICR
PR00541 MUSCRINICM4R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACM4_MOUSE
AccessioniPrimary (citable) accession number: P32211
Secondary accession number(s): Q64056
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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Main funding by: National Institutes of Health

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