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UniProtKB - P32179 (MET22_YEAST)
Protein
3'(2'),5'-bisphosphate nucleotidase
Gene
MET22
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance. Confers resistance to lithium.
Miscellaneous
Present with 7330 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.1.3.7
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 72 | Magnesium 1 | 1 | |
Binding sitei | 72 | SubstrateBy similarity | 1 | |
Metal bindingi | 142 | Magnesium 1 | 1 | |
Metal bindingi | 142 | Magnesium 2 | 1 | |
Metal bindingi | 144 | Magnesium 1; via carbonyl oxygen | 1 | |
Metal bindingi | 145 | Magnesium 2 | 1 | |
Metal bindingi | 294 | Magnesium 2 | 1 | |
Binding sitei | 294 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 3'(2'),5'-bisphosphate nucleotidase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- phosphoric ester hydrolase activity Source: GO_Central
GO - Biological processi
- hyperosmotic salinity response Source: SGD
- inositol phosphate dephosphorylation Source: InterPro
- methionine biosynthetic process Source: SGD
- phosphatidylinositol phosphate biosynthetic process Source: InterPro
- sulfate assimilation Source: SGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Stress response |
Ligand | Lithium, Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.7, 984 |
SABIO-RKi | P32179 |
Names & Taxonomyi
Protein namesi | Recommended name: 3'(2'),5'-bisphosphate nucleotidase (EC:3.1.3.7)Alternative name(s): 3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase DPNPase Halotolerance protein HAL2 Methionine-requiring protein 22 |
Gene namesi | Name:MET22 Synonyms:HAL2 Ordered Locus Names:YOL064C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005425, MET22 |
VEuPathDBi | FungiDB:YOL064C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142537 | 1 – 357 | 3'(2'),5'-bisphosphate nucleotidaseAdd BLAST | 357 |
Proteomic databases
MaxQBi | P32179 |
PaxDbi | P32179 |
PRIDEi | P32179 |
PTM databases
iPTMneti | P32179 |
Expressioni
Inductioni
By salt stress.
Interactioni
Protein-protein interaction databases
BioGRIDi | 34337, 393 interactors |
DIPi | DIP-4072N |
IntActi | P32179, 2 interactors |
MINTi | P32179 |
STRINGi | 4932.YOL064C |
Miscellaneous databases
RNActi | P32179, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32179 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32179 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 147 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the inositol monophosphatase superfamily.Curated
Phylogenomic databases
eggNOGi | KOG1528, Eukaryota |
HOGENOMi | CLU_033446_2_1_1 |
OMAi | PISMRPV |
Family and domain databases
InterProi | View protein in InterPro IPR006239, Bisphos_HAL2 IPR020583, Inositol_monoP_metal-BS IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES |
TIGRFAMsi | TIGR01330, bisphos_HAL2, 1 hit |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32179-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALERELLVA TQAVRKASLL TKRIQSEVIS HKDSTTITKN DNSPVTTGDY
60 70 80 90 100
AAQTIIINAI KSNFPDDKVV GEESSSGLSD AFVSGILNEI KANDEVYNKN
110 120 130 140 150
YKKDDFLFTN DQFPLKSLED VRQIIDFGNY EGGRKGRFWC LDPIDGTKGF
160 170 180 190 200
LRGEQFAVCL ALIVDGVVQL GCIGCPNLVL SSYGAQDLKG HESFGYIFRA
210 220 230 240 250
VRGLGAFYSP SSDAESWTKI HVRHLKDTKD MITLEGVEKG HSSHDEQTAI
260 270 280 290 300
KNKLNISKSL HLDSQAKYCL LALGLADVYL RLPIKLSYQE KIWDHAAGNV
310 320 330 340 350
IVHEAGGIHT DAMEDVPLDF GNGRTLATKG VIASSGPREL HDLVVSTSCD
VIQSRNA
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 40 | N → S in strain: Montrache. | 1 | |
Natural varianti | 61 | K → M in strain: Montrache. | 1 | |
Natural varianti | 63 | N → S in strain: Montrache. | 1 | |
Natural varianti | 308 | I → V in strain: Montrache. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72847 Genomic DNA Translation: CAA51361.1 AY500154 Genomic DNA Translation: AAR89916.1 Z74806 Genomic DNA Translation: CAA99074.1 BK006948 Genomic DNA Translation: DAA10719.1 |
PIRi | S35318 |
RefSeqi | NP_014577.1, NM_001183319.1 |
Genome annotation databases
EnsemblFungii | YOL064C_mRNA; YOL064C; YOL064C |
GeneIDi | 854090 |
KEGGi | sce:YOL064C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72847 Genomic DNA Translation: CAA51361.1 AY500154 Genomic DNA Translation: AAR89916.1 Z74806 Genomic DNA Translation: CAA99074.1 BK006948 Genomic DNA Translation: DAA10719.1 |
PIRi | S35318 |
RefSeqi | NP_014577.1, NM_001183319.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K9Y | X-ray | 1.90 | A | 1-357 | [»] | |
1K9Z | X-ray | 1.50 | A | 1-357 | [»] | |
1KA0 | X-ray | 1.80 | A | 1-357 | [»] | |
1KA1 | X-ray | 1.30 | A | 1-357 | [»] | |
1QGX | X-ray | 1.60 | A | 1-357 | [»] | |
SMRi | P32179 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34337, 393 interactors |
DIPi | DIP-4072N |
IntActi | P32179, 2 interactors |
MINTi | P32179 |
STRINGi | 4932.YOL064C |
PTM databases
iPTMneti | P32179 |
Proteomic databases
MaxQBi | P32179 |
PaxDbi | P32179 |
PRIDEi | P32179 |
Genome annotation databases
EnsemblFungii | YOL064C_mRNA; YOL064C; YOL064C |
GeneIDi | 854090 |
KEGGi | sce:YOL064C |
Organism-specific databases
SGDi | S000005425, MET22 |
VEuPathDBi | FungiDB:YOL064C |
Phylogenomic databases
eggNOGi | KOG1528, Eukaryota |
HOGENOMi | CLU_033446_2_1_1 |
OMAi | PISMRPV |
Enzyme and pathway databases
BRENDAi | 3.1.3.7, 984 |
SABIO-RKi | P32179 |
Miscellaneous databases
EvolutionaryTracei | P32179 |
PROi | PR:P32179 |
RNActi | P32179, protein |
Family and domain databases
InterProi | View protein in InterPro IPR006239, Bisphos_HAL2 IPR020583, Inositol_monoP_metal-BS IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES |
TIGRFAMsi | TIGR01330, bisphos_HAL2, 1 hit |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MET22_YEAST | |
Accessioni | P32179Primary (citable) accession number: P32179 Secondary accession number(s): D6W203, Q6RFY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | September 29, 2021 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families