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Protein

Paired box protein Pax-4

Gene

Pax4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that competes with PAX6 in binding to a common element in the glucagon, insulin and somatostatin promoters.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi170 – 229HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Paired box protein Pax-4
Gene namesi
Name:Pax4
Synonyms:Pax-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:97488 Pax4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi272 – 273EP → DL: Loss of transactivation function. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000501811 – 349Paired box protein Pax-4Add BLAST349

Proteomic databases

PaxDbiP32115
PRIDEiP32115

PTM databases

PhosphoSitePlusiP32115

Expressioni

Tissue specificityi

Expressed in early pancreas. Later restricted to beta cells. Undetectable in adult islets.

Gene expression databases

BgeeiENSMUSG00000029706 Expressed in 60 organ(s), highest expression level in head
CleanExiMM_PAX4
ExpressionAtlasiP32115 baseline and differential
GenevisibleiP32115 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031718

Structurei

3D structure databases

ProteinModelPortaliP32115
SMRiP32115
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 131PairedPROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 349Transcription repressionAdd BLAST72

Sequence similaritiesi

Belongs to the paired homeobox family.Curated

Keywords - Domaini

Homeobox, Paired box

Phylogenomic databases

eggNOGiKOG0849 Eukaryota
ENOG410XS01 LUCA
GeneTreeiENSGT00680000099553
HOGENOMiHOG000230939
HOVERGENiHBG009115
InParanoidiP32115
KOiK08032
OrthoDBiEOG091G0S4E
PhylomeDBiP32115
TreeFamiTF320146

Family and domain databases

CDDicd00086 homeodomain, 1 hit
Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR001523 Paired_dom
IPR033559 Pax-4
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR24329:SF14 PTHR24329:SF14, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00292 PAX, 1 hit
PRINTSiPR00027 PAIREDBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00351 PAX, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00034 PAIRED_1, 1 hit
PS51057 PAIRED_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P32115-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS
60 70 80 90 100
NGCVSKILGR YYRTGVLEPK CIGGSKPRLA TPAVVARIAQ LKDEYPALFA
110 120 130 140 150
WEIQHQLCTE GLCTQDKAPS VSSINRVLRA LQEDQSLHWT QLRSPAVLAP
160 170 180 190 200
VLPSPHSNCG APRGPHPGTS HRNRTIFSPG QAEALEKEFQ RGQYPDSVAR
210 220 230 240 250
GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWEAQLPGA SQDLTVPKNS
260 270 280 290 300
PGIISAQQSP GSVPSAALPV LEPLSPSFCQ LCCGTAPGRC SSDTSSQAYL
310 320 330 340
QPYWDCQSLL PVASSSYVEF AWPCLTTHPV HHLIGGPGQV PSTHCSNWP
Length:349
Mass (Da):38,008
Last modified:May 30, 2000 - v2
Checksum:i215E0469F46DF49B
GO
Isoform 2 (identifier: P32115-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-251: Missing.

Show »
Length:336
Mass (Da):36,713
Checksum:i15B04846791607E4
GO
Isoform 3 (identifier: P32115-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-314: Missing.

Show »
Length:312
Mass (Da):34,039
Checksum:i9FA23A25741CB4A8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZNC8B7ZNC8_MOUSE
Paired box protein Pax-4
Pax4
335Annotation score:
G3UZE8G3UZE8_MOUSE
Paired box protein Pax-4
Pax4
307Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175T → A in CAA70772 (PubMed:9121556).Curated1
Sequence conflicti277S → P in AAF14073 (PubMed:10567552).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002361239 – 251Missing in isoform 2. 2 PublicationsAdd BLAST13
Alternative sequenceiVSP_002362278 – 314Missing in isoform 3. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031150 mRNA Translation: AAC40046.1
AB008912 mRNA Translation: BAA24505.1
AB010557 mRNA Translation: BAA24516.1
AB010558 mRNA Translation: BAA24517.1
AF145233 mRNA Translation: AAF03533.1
AF142321 Genomic DNA Translation: AAF03530.1
BC145168 mRNA Translation: AAI45169.1
AF104231 mRNA Translation: AAF14073.1
Y09584 mRNA Translation: CAA70772.1
CCDSiCCDS39447.1 [P32115-1]
CCDS51732.1 [P32115-2]
PIRiA41061
JC5827
JC5961
JC5962
RefSeqiNP_001153397.1, NM_001159925.1 [P32115-2]
NP_001153398.1, NM_001159926.1
NP_035168.1, NM_011038.2 [P32115-1]
XP_017176939.1, XM_017321450.1 [P32115-1]
UniGeneiMm.8026

Genome annotation databases

EnsembliENSMUST00000031718; ENSMUSP00000031718; ENSMUSG00000029706 [P32115-1]
ENSMUST00000164519; ENSMUSP00000131301; ENSMUSG00000029706 [P32115-2]
GeneIDi18506
KEGGimmu:18506
UCSCiuc009bcs.2 mouse [P32115-1]
uc012eit.1 mouse [P32115-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031150 mRNA Translation: AAC40046.1
AB008912 mRNA Translation: BAA24505.1
AB010557 mRNA Translation: BAA24516.1
AB010558 mRNA Translation: BAA24517.1
AF145233 mRNA Translation: AAF03533.1
AF142321 Genomic DNA Translation: AAF03530.1
BC145168 mRNA Translation: AAI45169.1
AF104231 mRNA Translation: AAF14073.1
Y09584 mRNA Translation: CAA70772.1
CCDSiCCDS39447.1 [P32115-1]
CCDS51732.1 [P32115-2]
PIRiA41061
JC5827
JC5961
JC5962
RefSeqiNP_001153397.1, NM_001159925.1 [P32115-2]
NP_001153398.1, NM_001159926.1
NP_035168.1, NM_011038.2 [P32115-1]
XP_017176939.1, XM_017321450.1 [P32115-1]
UniGeneiMm.8026

3D structure databases

ProteinModelPortaliP32115
SMRiP32115
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031718

PTM databases

PhosphoSitePlusiP32115

Proteomic databases

PaxDbiP32115
PRIDEiP32115

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031718; ENSMUSP00000031718; ENSMUSG00000029706 [P32115-1]
ENSMUST00000164519; ENSMUSP00000131301; ENSMUSG00000029706 [P32115-2]
GeneIDi18506
KEGGimmu:18506
UCSCiuc009bcs.2 mouse [P32115-1]
uc012eit.1 mouse [P32115-2]

Organism-specific databases

CTDi5078
MGIiMGI:97488 Pax4

Phylogenomic databases

eggNOGiKOG0849 Eukaryota
ENOG410XS01 LUCA
GeneTreeiENSGT00680000099553
HOGENOMiHOG000230939
HOVERGENiHBG009115
InParanoidiP32115
KOiK08032
OrthoDBiEOG091G0S4E
PhylomeDBiP32115
TreeFamiTF320146

Miscellaneous databases

PROiPR:P32115
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029706 Expressed in 60 organ(s), highest expression level in head
CleanExiMM_PAX4
ExpressionAtlasiP32115 baseline and differential
GenevisibleiP32115 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR001523 Paired_dom
IPR033559 Pax-4
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR24329:SF14 PTHR24329:SF14, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00292 PAX, 1 hit
PRINTSiPR00027 PAIREDBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00351 PAX, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00034 PAIRED_1, 1 hit
PS51057 PAIRED_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAX4_MOUSE
AccessioniPrimary (citable) accession number: P32115
Secondary accession number(s): B7ZNC7
, P97341, Q9QUR1, Q9R091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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