UniProtKB - P32089 (TXTP_RAT)
Protein
Tricarboxylate transport protein, mitochondrial
Gene
Slc25a1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Citrate transporter that mediates the exchange of mitochondrial citrate for cytosolic malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis- but not trans-aconitate and to a lesser extend maleate and succinate. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD+ for the glycolytic pathway. Required for proper neuromuscular junction formation.By similarity
GO - Molecular functioni
- citrate secondary active transmembrane transporter activity Source: RGD
- citrate transmembrane transporter activity Source: RGD
GO - Biological processi
- mitochondrial citrate transmembrane transport Source: GO_Central
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-RNO-70263, Gluconeogenesis R-RNO-75105, Fatty acyl-CoA biosynthesis |
Protein family/group databases
TCDBi | 2.A.29.7.1, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Tricarboxylate transport protein, mitochondrialAlternative name(s): Citrate transport protein Short name: CTP Solute carrier family 25 member 1 Tricarboxylate carrier protein |
Gene namesi | Name:Slc25a1 Synonyms:Slc20a3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3703, Slc25a1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein Sequence analysis
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrion Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 29 – 46 | Helical; Name=1Sequence analysisAdd BLAST | 18 | |
Transmembranei | 86 – 105 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Transmembranei | 129 – 143 | Helical; Name=3Sequence analysisAdd BLAST | 15 | |
Transmembranei | 183 – 202 | Helical; Name=4Sequence analysisAdd BLAST | 20 | |
Transmembranei | 224 – 241 | Helical; Name=5Sequence analysisAdd BLAST | 18 | |
Transmembranei | 278 – 297 | Helical; Name=6Sequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 13 | Mitochondrion1 PublicationAdd BLAST | 13 | |
ChainiPRO_0000019263 | 14 – 311 | Tricarboxylate transport protein, mitochondrialAdd BLAST | 298 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 156 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P32089 |
PaxDbi | P32089 |
PRIDEi | P32089 |
PTM databases
CarbonylDBi | P32089 |
iPTMneti | P32089 |
PhosphoSitePlusi | P32089 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000038001, Expressed in female gonad and 21 other tissues |
Genevisiblei | P32089, RN |
Interactioni
Protein-protein interaction databases
BioGRIDi | 248354, 2 interactors |
IntActi | P32089, 3 interactors |
STRINGi | 10116.ENSRNOP00000000306 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 23 – 111 | Solcar 1Add BLAST | 89 | |
Repeati | 122 – 208 | Solcar 2Add BLAST | 87 | |
Repeati | 218 – 303 | Solcar 3Add BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0756, Eukaryota |
GeneTreei | ENSGT00550000074856 |
HOGENOMi | CLU_015166_5_1_1 |
InParanoidi | P32089 |
OMAi | FLSNKMR |
OrthoDBi | 1523221at2759 |
PhylomeDBi | P32089 |
TreeFami | TF105786 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32089-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPRAPRAL TAAAPGSGKA KLTHPGKAIL AGGLAGGIEI CITFPTEYVK
60 70 80 90 100
TQLQLDERAN PPRYRGIGDC VRQTVRSHGV LGLYRGLSSL LYGSIPKAAV
110 120 130 140 150
RFGMFEFLSN HMRDAQGRLD SRRGLLCGLG AGVAEAVVVV CPMETVKVKF
160 170 180 190 200
IHDQTSSNPK YRGFFHGVRE IVREQGLKGT YQGLTATVLK QGSNQAIRFF
210 220 230 240 250
VMTSLRNWYQ GDNPNKPMNP LITGVFGAVA GAASVFGNTP LDVIKTRMQG
260 270 280 290 300
LEAHKYRNTL DCGVQILKNE GPKAFYKGTV PRLGRVCLDV AIVFVIYDEV
310
VKLLNKVWKT D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12016 mRNA Translation: AAA18899.1 BC100077 mRNA Translation: AAI00078.1 |
PIRi | A46595 |
RefSeqi | NP_059003.1, NM_017307.3 |
Genome annotation databases
Ensembli | ENSRNOT00000000306; ENSRNOP00000000306; ENSRNOG00000038001 |
GeneIDi | 29743 |
KEGGi | rno:29743 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12016 mRNA Translation: AAA18899.1 BC100077 mRNA Translation: AAI00078.1 |
PIRi | A46595 |
RefSeqi | NP_059003.1, NM_017307.3 |
3D structure databases
SMRi | P32089 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248354, 2 interactors |
IntActi | P32089, 3 interactors |
STRINGi | 10116.ENSRNOP00000000306 |
Protein family/group databases
TCDBi | 2.A.29.7.1, the mitochondrial carrier (mc) family |
PTM databases
CarbonylDBi | P32089 |
iPTMneti | P32089 |
PhosphoSitePlusi | P32089 |
Proteomic databases
jPOSTi | P32089 |
PaxDbi | P32089 |
PRIDEi | P32089 |
Genome annotation databases
Ensembli | ENSRNOT00000000306; ENSRNOP00000000306; ENSRNOG00000038001 |
GeneIDi | 29743 |
KEGGi | rno:29743 |
Organism-specific databases
CTDi | 6576 |
RGDi | 3703, Slc25a1 |
Phylogenomic databases
eggNOGi | KOG0756, Eukaryota |
GeneTreei | ENSGT00550000074856 |
HOGENOMi | CLU_015166_5_1_1 |
InParanoidi | P32089 |
OMAi | FLSNKMR |
OrthoDBi | 1523221at2759 |
PhylomeDBi | P32089 |
TreeFami | TF105786 |
Enzyme and pathway databases
Reactomei | R-RNO-70263, Gluconeogenesis R-RNO-75105, Fatty acyl-CoA biosynthesis |
Miscellaneous databases
PROi | PR:P32089 |
Gene expression databases
Bgeei | ENSRNOG00000038001, Expressed in female gonad and 21 other tissues |
Genevisiblei | P32089, RN |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TXTP_RAT | |
Accessioni | P32089Primary (citable) accession number: P32089 Secondary accession number(s): Q498T8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families