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UniProtKB - P32081 (CSPB_BACSU)
Protein
Cold shock protein CspB
Gene
cspB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
1 PublicationGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- nucleic acid binding Source: GO_Central
- RNA binding Source: GO_Central
- transcription antitermination factor activity, RNA binding Source: GO_Central
GO - Biological processi
- negative regulation of DNA-templated transcription, termination Source: GO_Central
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Stress response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU09100-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cold shock protein CspBAlternative name(s): Major cold shock protein |
Gene namesi | Name:cspB Synonyms:cspA Ordered Locus Names:BSU09100 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Other locations
- nucleoid 1 Publication
Note: Shows transcription-dependent localization at subcellular sites surrounding the nucleoid.
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
A double cshB-cspB disruption mutant cannot be made.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100295 | 1 – 67 | Cold shock protein CspBAdd BLAST | 67 |
Proteomic databases
jPOSTi | P32081 |
PaxDbi | P32081 |
PRIDEi | P32081 |
Expressioni
Inductioni
In response to low temperature.
Interactioni
Subunit structurei
Homodimer.
Interacts with RNA helicase CshB when cells are transcriptionally active.
1 PublicationProtein-protein interaction databases
STRINGi | 224308.BSU09100 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32081 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32081 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 63 | CSDAdd BLAST | 60 |
Phylogenomic databases
eggNOGi | COG1278, Bacteria |
InParanoidi | P32081 |
OMAi | HYSTIKM |
PhylomeDBi | P32081 |
Family and domain databases
CDDi | cd04458, CSP_CDS, 1 hit |
InterProi | View protein in InterPro IPR012156, Cold_shock_CspA IPR011129, CSD IPR019844, CSD_1 IPR002059, CSP_DNA-bd IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF00313, CSD, 1 hit |
PIRSFi | PIRSF002599, Cold_shock_A, 1 hit |
PRINTSi | PR00050, COLDSHOCK |
SMARTi | View protein in SMART SM00357, CSP, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00352, CSD_1, 1 hit PS51857, CSD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32081-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEGKVKWFN SEKGFGFIEV EGQDDVFVHF SAIQGEGFKT LEEGQAVSFE
60
IVEGNRGPQA ANVTKEA
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 17 | F → L in strain: Globigii. | 1 | |
Natural varianti | 44 | G → S in strain: Globigii. | 1 | |
Natural varianti | 46 | A → S. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59715 Genomic DNA Translation: CAA42235.1 U58859 Genomic DNA Translation: AAB01346.1 X96983 Genomic DNA Translation: CAA65693.1 AL009126 Genomic DNA Translation: CAB12738.1 U60029 Genomic DNA Translation: AAC80233.1 U60030 Genomic DNA Translation: AAC80234.1 X73463 Genomic DNA Translation: CAA51842.1 |
PIRi | A45723 |
RefSeqi | NP_388791.1, NC_000964.3 WP_003224086.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12738; CAB12738; BSU_09100 |
GeneIDi | 64302783 936224 |
KEGGi | bsu:BSU09100 |
PATRICi | fig|224308.179.peg.983 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59715 Genomic DNA Translation: CAA42235.1 U58859 Genomic DNA Translation: AAB01346.1 X96983 Genomic DNA Translation: CAA65693.1 AL009126 Genomic DNA Translation: CAB12738.1 U60029 Genomic DNA Translation: AAC80233.1 U60030 Genomic DNA Translation: AAC80234.1 X73463 Genomic DNA Translation: CAA51842.1 |
PIRi | A45723 |
RefSeqi | NP_388791.1, NC_000964.3 WP_003224086.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CSP | X-ray | 2.45 | A | 1-67 | [»] | |
1CSQ | X-ray | 2.70 | A | 1-67 | [»] | |
1NMF | NMR | - | A | 1-67 | [»] | |
1NMG | NMR | - | A | 1-67 | [»] | |
2ES2 | X-ray | 1.78 | A | 1-67 | [»] | |
2F52 | NMR | - | A | 1-67 | [»] | |
2I5L | X-ray | 2.55 | X | 1-67 | [»] | |
2I5M | X-ray | 2.30 | X | 1-67 | [»] | |
3PF4 | X-ray | 1.38 | A/B | 1-67 | [»] | |
3PF5 | X-ray | 1.68 | A/B | 1-67 | [»] | |
6SZZ | X-ray | 2.05 | A | 1-67 | [»] | |
6T00 | X-ray | 2.10 | A | 1-66 | [»] | |
SMRi | P32081 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU09100 |
Proteomic databases
jPOSTi | P32081 |
PaxDbi | P32081 |
PRIDEi | P32081 |
Genome annotation databases
EnsemblBacteriai | CAB12738; CAB12738; BSU_09100 |
GeneIDi | 64302783 936224 |
KEGGi | bsu:BSU09100 |
PATRICi | fig|224308.179.peg.983 |
Phylogenomic databases
eggNOGi | COG1278, Bacteria |
InParanoidi | P32081 |
OMAi | HYSTIKM |
PhylomeDBi | P32081 |
Enzyme and pathway databases
BioCyci | BSUB:BSU09100-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P32081 |
PROi | PR:P32081 |
Family and domain databases
CDDi | cd04458, CSP_CDS, 1 hit |
InterProi | View protein in InterPro IPR012156, Cold_shock_CspA IPR011129, CSD IPR019844, CSD_1 IPR002059, CSP_DNA-bd IPR012340, NA-bd_OB-fold |
Pfami | View protein in Pfam PF00313, CSD, 1 hit |
PIRSFi | PIRSF002599, Cold_shock_A, 1 hit |
PRINTSi | PR00050, COLDSHOCK |
SMARTi | View protein in SMART SM00357, CSP, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS00352, CSD_1, 1 hit PS51857, CSD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CSPB_BACSU | |
Accessioni | P32081Primary (citable) accession number: P32081 Secondary accession number(s): P41017, Q45690 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | September 29, 2021 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references