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Entry version 137 (13 Nov 2019)
Sequence version 2 (01 Feb 1994)
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Protein

Prophage integrase IntA

Gene

intA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Part of the cryptic P4-like prophage CP4-57, it excises the prophage when overexpressed, which also requires integration host factor (encoded by ihfA and ihfB) (PubMed:7511583). Overexpression of AlpA leads to excision of the CP4-57 prophage, which inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA which is required to rescue stalled ribosomes in a process known as trans-translation (PubMed:7511582).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei248PROSITE-ProRule annotation1
Active sitei275PROSITE-ProRule annotation1
Active sitei337PROSITE-ProRule annotation1
Active sitei340PROSITE-ProRule annotation1
Active sitei363PROSITE-ProRule annotation1
Active sitei373O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • prophage integrase activity Source: EcoCyc

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding
Biological processDNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG11783-MONOMER
ECOL316407:JW2602-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Prophage integrase IntACurated
Alternative name(s):
Prophage CP4-57 integrase SlpA1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:intA
Synonyms:intX, slpA1 Publication
Ordered Locus Names:b2622, JW2602
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Loss of excision of its prophage (PubMed:7511583).1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001975111 – 413Prophage integrase IntAAdd BLAST413

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32053

PRoteomics IDEntifications database

More...
PRIDEi
P32053

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By AlpA (PubMed:7511582).1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4260622, 13 interactors
850520, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-10034N

Protein interaction database and analysis system

More...
IntActi
P32053, 26 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2622

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P32053

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini105 – 186Core-binding (CB)PROSITE-ProRule annotationAdd BLAST82
Domaini209 – 386Tyr recombinasePROSITE-ProRule annotationAdd BLAST178

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105DFP Bacteria
COG0582 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000263105

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32053

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P32053

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.150.130, 1 hit
1.10.443.10, 1 hit
3.30.160.390, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR002104 Integrase_catalytic
IPR038488 Integrase_DNA-bd_sf
IPR025166 Integrase_DNA_bind_dom
IPR010998 Integrase_recombinase_N

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13356 Arm-DNA-bind_3, 1 hit
PF00589 Phage_integrase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56349 SSF56349, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51900 CB, 1 hit
PS51898 TYR_RECOMBINASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32053-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARKTKPLTD TEIKAAKPKD ADYQLYDGDG LTLLIKSSGS KLWQFRYYRP
60 70 80 90 100
LTKQRTKQSF GAYPAVSLSD ARKLRAESKV LLAKDIDPQE HQKEQVRNSQ
110 120 130 140 150
EAKTNTFLLV AERWWNVKKT SVTEDYADDI WRSLERDIFP AIGDISITEI
160 170 180 190 200
KAHTLVKAVQ PVQARGALET VRRLCQRINE VMIYAQNTGL IDAVPSVNIG
210 220 230 240 250
KAFEKPQKKN MPSIRPDQLP QLMHTMRTAS ISMSTRCLFM WQLLTITRPA
260 270 280 290 300
EAAEARWDEI DFNASEWKIP AARMKMNRDH TVPLSDGALA ILEMMKPLSG
310 320 330 340 350
GREFIFPSRI KPNQPMNSQT VNAALKRAGL GGVLVSHGLR SIASTALNEE
360 370 380 390 400
GFPPDVIEAA LAHVDKNEVR RAYNRSDYLE QRRPMMQWWA DLVKAADSGS
410
IVLTHLSKIR LVG
Length:413
Mass (Da):46,652
Last modified:February 1, 1994 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE050A1F60860BCB5
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U03737 Unassigned DNA Translation: AAA18417.1
U36840 Genomic DNA Translation: AAA79791.1
U00096 Genomic DNA Translation: AAC75670.1
AP009048 Genomic DNA Translation: BAA20921.2
D12501 Genomic DNA Translation: BAA20981.1

Protein sequence database of the Protein Information Resource

More...
PIRi
H65040

NCBI Reference Sequences

More...
RefSeqi
NP_417111.1, NC_000913.3
WP_000101723.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75670; AAC75670; b2622
BAA20921; BAA20921; BAA20921

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946160

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2602
eco:b2622

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.4117

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03737 Unassigned DNA Translation: AAA18417.1
U36840 Genomic DNA Translation: AAA79791.1
U00096 Genomic DNA Translation: AAC75670.1
AP009048 Genomic DNA Translation: BAA20921.2
D12501 Genomic DNA Translation: BAA20981.1
PIRiH65040
RefSeqiNP_417111.1, NC_000913.3
WP_000101723.1, NZ_LN832404.1

3D structure databases

SMRiP32053
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4260622, 13 interactors
850520, 1 interactor
DIPiDIP-10034N
IntActiP32053, 26 interactors
STRINGi511145.b2622

Proteomic databases

PaxDbiP32053
PRIDEiP32053

Genome annotation databases

EnsemblBacteriaiAAC75670; AAC75670; b2622
BAA20921; BAA20921; BAA20921
GeneIDi946160
KEGGiecj:JW2602
eco:b2622
PATRICifig|1411691.4.peg.4117

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1731

Phylogenomic databases

eggNOGiENOG4105DFP Bacteria
COG0582 LUCA
HOGENOMiHOG000263105
InParanoidiP32053
PhylomeDBiP32053

Enzyme and pathway databases

BioCyciEcoCyc:EG11783-MONOMER
ECOL316407:JW2602-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32053

Family and domain databases

Gene3Di1.10.150.130, 1 hit
1.10.443.10, 1 hit
3.30.160.390, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR002104 Integrase_catalytic
IPR038488 Integrase_DNA-bd_sf
IPR025166 Integrase_DNA_bind_dom
IPR010998 Integrase_recombinase_N
PfamiView protein in Pfam
PF13356 Arm-DNA-bind_3, 1 hit
PF00589 Phage_integrase, 1 hit
SUPFAMiSSF56349 SSF56349, 1 hit
PROSITEiView protein in PROSITE
PS51900 CB, 1 hit
PS51898 TYR_RECOMBINASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiINTA_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32053
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: November 13, 2019
This is version 137 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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