UniProtKB - P32048 (SYKM_YEAST)
Protein
Lysine--tRNA ligase, mitochondrial
Gene
MSK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 2280 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.1.1.6
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- lysine-tRNA ligase activity Source: SGD
- tRNA binding Source: GO_Central
GO - Biological processi
- lysyl-tRNA aminoacylation Source: SGD
- mitochondrial lysyl-tRNA aminoacylation Source: SGD
- mitochondrial translation Source: SGD
- tRNA processing Source: SGD
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine--tRNA ligase, mitochondrial (EC:6.1.1.6)Alternative name(s): Lysyl-tRNA synthetase Short name: LysRS |
Gene namesi | Name:MSK1 Ordered Locus Names:YNL073W ORF Names:N2364 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005017, MSK1 |
VEuPathDBi | FungiDB:YNL073W |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 30 | MitochondrionSequence analysisAdd BLAST | 30 | |
ChainiPRO_0000035812 | 31 – 576 | Lysine--tRNA ligase, mitochondrialAdd BLAST | 546 |
Proteomic databases
MaxQBi | P32048 |
PaxDbi | P32048 |
PRIDEi | P32048 |
PTM databases
iPTMneti | P32048 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35750, 88 interactors |
DIPi | DIP-5319N |
IntActi | P32048, 2 interactors |
MINTi | P32048 |
STRINGi | 4932.YNL073W |
Miscellaneous databases
RNActi | P32048, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1885, Eukaryota |
GeneTreei | ENSGT01020000230546 |
HOGENOMi | CLU_008255_6_0_1 |
InParanoidi | P32048 |
OMAi | CEFYHAY |
Family and domain databases
CDDi | cd04322, LysRS_N, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P32048-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNVLLKRRSL TFAPRWLWCK CRSSRSRPYS LAHAVDTSKM EATRRNGQIV
60 70 80 90 100
KDLGRYYPSM SESALHDLCQ EYKEVTIADF NERFLGNPAT LHHEDNPNLL
110 120 130 140 150
LSINGRIKSI RFSGQKIVFI DLYNGSSGLK NDTQLQLIVN YNKIGGSSED
160 170 180 190 200
KANFSEYMNF LKKGDYIKAL GYPGFSQSRV KMLSLICNKL PIVLSVSQLP
210 220 230 240 250
LPSRLNDETK IKSNRVVDYQ LNGTQTLLVR ARIIKLLRKF LDDRNFVEVE
260 270 280 290 300
TPILSSKSNG AMAKPFITSS KDFDHLELRI APELWLKRLI ISGLQKVYEI
310 320 330 340 350
GKVFRNEGID STHNAEFSTL EFYETYMSMD DIVTRTEDLF KFLITNLQKF
360 370 380 390 400
FQDTRLPVPK TFSELHLALS ENNWKFRKVE FLPTLNKELG IDLMNSGLDI
410 420 430 440 450
NKPSELLKAL PKDIAKKYFP SADNTGQLSS LQILNKLSDV FLEQRHCQST
460 470 480 490 500
LPTVIYHQPA ILSPLAKTDP QNKQVTKRFE VFIKGKEYIN AYEEENCPQL
510 520 530 540 550
QLQKFLQQKQ INELTGNKTE TLSPVIDYQY VETMKYGMPP VGGFGLGIDR
560 570
LCMLFCDKKR IEEVLPFGCV DDVNRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57360 Genomic DNA Translation: CAA40634.1 Sequence problems. X86470 Genomic DNA Translation: CAA60187.1 Z71349 Genomic DNA Translation: CAA95947.1 BK006947 Genomic DNA Translation: DAA10472.1 |
PIRi | S14834 |
RefSeqi | NP_014326.1, NM_001182911.1 |
Genome annotation databases
EnsemblFungii | YNL073W_mRNA; YNL073W; YNL073W |
GeneIDi | 855651 |
KEGGi | sce:YNL073W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57360 Genomic DNA Translation: CAA40634.1 Sequence problems. X86470 Genomic DNA Translation: CAA60187.1 Z71349 Genomic DNA Translation: CAA95947.1 BK006947 Genomic DNA Translation: DAA10472.1 |
PIRi | S14834 |
RefSeqi | NP_014326.1, NM_001182911.1 |
3D structure databases
SMRi | P32048 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35750, 88 interactors |
DIPi | DIP-5319N |
IntActi | P32048, 2 interactors |
MINTi | P32048 |
STRINGi | 4932.YNL073W |
PTM databases
iPTMneti | P32048 |
Proteomic databases
MaxQBi | P32048 |
PaxDbi | P32048 |
PRIDEi | P32048 |
Genome annotation databases
EnsemblFungii | YNL073W_mRNA; YNL073W; YNL073W |
GeneIDi | 855651 |
KEGGi | sce:YNL073W |
Organism-specific databases
SGDi | S000005017, MSK1 |
VEuPathDBi | FungiDB:YNL073W |
Phylogenomic databases
eggNOGi | KOG1885, Eukaryota |
GeneTreei | ENSGT01020000230546 |
HOGENOMi | CLU_008255_6_0_1 |
InParanoidi | P32048 |
OMAi | CEFYHAY |
Miscellaneous databases
PROi | PR:P32048 |
RNActi | P32048, protein |
Family and domain databases
CDDi | cd04322, LysRS_N, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR002313, Lys-tRNA-ligase_II IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYKM_YEAST | |
Accessioni | P32048Primary (citable) accession number: P32048 Secondary accession number(s): D6W1A6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | February 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families