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Protein

Lysine--tRNA ligase, mitochondrial

Gene

MSK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 2280 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lysine-tRNA ligase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • lysyl-tRNA aminoacylation Source: SGD
  • mitochondrial lysyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD
  • tRNA import into mitochondrion Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33102-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase, mitochondrial (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:MSK1
Ordered Locus Names:YNL073W
ORF Names:N2364
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL073W
SGDiS000005017 MSK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionSequence analysisAdd BLAST30
ChainiPRO_000003581231 – 576Lysine--tRNA ligase, mitochondrialAdd BLAST546

Proteomic databases

MaxQBiP32048
PaxDbiP32048
PRIDEiP32048

PTM databases

iPTMnetiP32048

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CRN1Q064402EBI-18739,EBI-4950

Protein-protein interaction databases

BioGridi35750, 86 interactors
DIPiDIP-5319N
IntActiP32048, 8 interactors
MINTiP32048
STRINGi4932.YNL073W

Structurei

3D structure databases

ProteinModelPortaliP32048
SMRiP32048
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000236577
InParanoidiP32048
KOiK04567
OMAiPAFRNEG
OrthoDBiEOG092C0ZU5

Family and domain databases

CDDicd00775 LysRS_core, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR002313 Lys-tRNA-ligase_II
IPR018149 Lys-tRNA-synth_II_C
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR00982 TRNASYNTHLYS
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00499 lysS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLLKRRSL TFAPRWLWCK CRSSRSRPYS LAHAVDTSKM EATRRNGQIV
60 70 80 90 100
KDLGRYYPSM SESALHDLCQ EYKEVTIADF NERFLGNPAT LHHEDNPNLL
110 120 130 140 150
LSINGRIKSI RFSGQKIVFI DLYNGSSGLK NDTQLQLIVN YNKIGGSSED
160 170 180 190 200
KANFSEYMNF LKKGDYIKAL GYPGFSQSRV KMLSLICNKL PIVLSVSQLP
210 220 230 240 250
LPSRLNDETK IKSNRVVDYQ LNGTQTLLVR ARIIKLLRKF LDDRNFVEVE
260 270 280 290 300
TPILSSKSNG AMAKPFITSS KDFDHLELRI APELWLKRLI ISGLQKVYEI
310 320 330 340 350
GKVFRNEGID STHNAEFSTL EFYETYMSMD DIVTRTEDLF KFLITNLQKF
360 370 380 390 400
FQDTRLPVPK TFSELHLALS ENNWKFRKVE FLPTLNKELG IDLMNSGLDI
410 420 430 440 450
NKPSELLKAL PKDIAKKYFP SADNTGQLSS LQILNKLSDV FLEQRHCQST
460 470 480 490 500
LPTVIYHQPA ILSPLAKTDP QNKQVTKRFE VFIKGKEYIN AYEEENCPQL
510 520 530 540 550
QLQKFLQQKQ INELTGNKTE TLSPVIDYQY VETMKYGMPP VGGFGLGIDR
560 570
LCMLFCDKKR IEEVLPFGCV DDVNRQ
Length:576
Mass (Da):66,128
Last modified:February 1, 1996 - v3
Checksum:iE3C440EEF86BB46E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57360 Genomic DNA Translation: CAA40634.1 Sequence problems.
X86470 Genomic DNA Translation: CAA60187.1
Z71349 Genomic DNA Translation: CAA95947.1
BK006947 Genomic DNA Translation: DAA10472.1
PIRiS14834
RefSeqiNP_014326.1, NM_001182911.1

Genome annotation databases

EnsemblFungiiYNL073W; YNL073W; YNL073W
GeneIDi855651
KEGGisce:YNL073W

Similar proteinsi

Entry informationi

Entry nameiSYKM_YEAST
AccessioniPrimary (citable) accession number: P32048
Secondary accession number(s): D6W1A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

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