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UniProtKB - P32009 (DNRF_STRPE)
Protein
Aklavinone 12-hydroxylase DnrF
Gene
dnrF
Organism
Streptomyces peucetius
Status
Functioni
Involved in the biosynthesis of the anthracyclines carminomycin, rhodomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the incorporation of a hydroxyl group at position C-11 of aklavinone, resulting in epsilon-rhodomycinone. It cannot accept substrates glycosylated at position C-7. It can also hydroxylate 11-deoxycarminomycinone and can use both NAD or NADP.
1 PublicationCatalytic activityi
- EC:1.14.13.1801 Publication
Cofactori
FADBy similarity
: daunorubicin biosynthesis Pathwayi
This protein is involved in the pathway daunorubicin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway daunorubicin biosynthesis and in Antibiotic biosynthesis.
Pathwayi: carminomycin biosynthesis
This protein is involved in the pathway carminomycin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway carminomycin biosynthesis and in Antibiotic biosynthesis.
Pathwayi: rhodomycin biosynthesis
This protein is involved in the pathway rhodomycin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway rhodomycin biosynthesis and in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | FADBy similarity | 1 | |
Binding sitei | 121 | FADBy similarity | 1 | |
Binding sitei | 145 | FAD; via amide nitrogenBy similarity | 1 | |
Active sitei | 224 | Proton acceptorBy similarity | 1 | |
Binding sitei | 308 | FADBy similarity | 1 | |
Binding sitei | 317 | Aklavinone; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 18 | FADBy similarity | 2 |
GO - Molecular functioni
- FAD binding Source: InterPro
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Antibiotic biosynthesis |
Ligand | FAD, Flavoprotein, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 1.14.13.180, 6073 |
UniPathwayi | UPA00054 UPA01040 UPA01042 |
Names & Taxonomyi
Protein namesi | Recommended name: Aklavinone 12-hydroxylase DnrF (EC:1.14.13.180)Alternative name(s): Aklavinone 11-hydroxylase |
Gene namesi | Name:dnrF |
Organismi | Streptomyces peucetius |
Taxonomic identifieri | 1950 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425676 | 1 – 489 | Aklavinone 12-hydroxylase DnrFAdd BLAST | 489 |
Interactioni
Subunit structurei
Monomer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 402 – 428 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 455 – 489 | DisorderedSequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 402 – 421 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the PheA/TfdB FAD monooxygenase family.Curated
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
P32009-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALTKPDVDV LVVGGGLGGL STALFLARRG ARVLLVERHA STSVLPKAAG
60 70 80 90 100
QNPRTMELFR FGGVADEILA TDDIRGAQGD FTIKVVERVG GRVLHSFAES
110 120 130 140 150
FEELVGATEQ CTPMPWALAP QDRVEPVLVA HAAKHGAEIR FATELTSFQA
160 170 180 190 200
GDDGVTARLR DLGTGAESTV SARYLVAADG PRSAIRESLG ITRHGHGTLA
210 220 230 240 250
HFMGVIFEAD LTAVVPPGST GWYYLQHPDF TGTFGPTDRP NRHTFYVATT
260 270 280 290 300
PERGERPEDY TPQRCTELIR LAVDAPGLVP DILDIQAWDM AAYIADRWRE
310 320 330 340 350
GPVLLVGDAA KVTPPTGGMG GNTAIGDGFD VAWKLAAVLR GEAGERLLDS
360 370 380 390 400
YGAERSLVSR LVVDESLAIY AQRMAPHLLG SVPEERGTAQ VVLGFRYRST
410 420 430 440 450
AVAAEDDDPE PTEDPRRPSG RPGFRAPHVW IEQDGTRRST VELFGDCWVL
460 470 480
LAAPEGGAWP GRPPAPPRIW ASASTSISSA AMSPPPPAN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18082 Genomic DNA Translation: AAC43342.1 M73758 Genomic DNA Translation: AAA74716.1 |
PIRi | S27706 |
Genome annotation databases
KEGGi | ag:AAC43342 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18082 Genomic DNA Translation: AAC43342.1 M73758 Genomic DNA Translation: AAA74716.1 |
PIRi | S27706 |
3D structure databases
SMRi | P32009 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAC43342 |
Enzyme and pathway databases
UniPathwayi | UPA00054 UPA01040 UPA01042 |
BRENDAi | 1.14.13.180, 6073 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNRF_STRPE | |
Accessioni | P32009Primary (citable) accession number: P32009 Secondary accession number(s): P72497 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | March 19, 2014 | |
Last modified: | June 2, 2021 | |
This is version 64 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families