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Protein

Macrosialin

Gene

Cd68

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Caution

CD68 is a commonly used marker for macrophages. However, a number of studies in human have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.Curated

GO - Biological processi

  • cellular response to organic substance Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Macrosialin
Alternative name(s):
CD_antigen: CD68
Gene namesi
Name:Cd68
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88342 Cd68

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 291ExtracellularSequence analysisAdd BLAST271
Transmembranei292 – 316HelicalPROSITE-ProRule annotationAdd BLAST25
Topological domaini317 – 326CytoplasmicPROSITE-ProRule annotation10

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001710321 – 326MacrosialinAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi129N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi139 ↔ 177PROSITE-ProRule annotation
Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi218N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi249 ↔ 286PROSITE-ProRule annotation
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N- and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP31996
PaxDbiP31996
PeptideAtlasiP31996
PRIDEiP31996

PTM databases

iPTMnetiP31996
PhosphoSitePlusiP31996
SwissPalmiP31996

Expressioni

Tissue specificityi

Expressed in tissue macrophages and to a lesser extent in dendritic cells.

Gene expression databases

BgeeiENSMUSG00000018774 Expressed in 185 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_CD68
ExpressionAtlasiP31996 baseline and differential
GenevisibleiP31996 MM

Interactioni

Protein-protein interaction databases

IntActiP31996, 1 interactor
MINTiP31996
STRINGi10090.ENSMUSP00000104294

Structurei

3D structure databases

ProteinModelPortaliP31996
SMRiP31996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati44 – 491-16
Repeati50 – 642-1Add BLAST15
Repeati65 – 721-28
Repeati73 – 882-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 109Mucin-likeAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi110 – 121Pro-rich (hinge)Add BLAST12

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818 Eukaryota
ENOG410XQ96 LUCA
GeneTreeiENSGT00530000063068
HOGENOMiHOG000070034
HOVERGENiHBG005287
InParanoidiP31996
KOiK06501
PhylomeDBiP31996
TreeFamiTF316339

Family and domain databases

InterProiView protein in InterPro
IPR018134 LAMP_CS
IPR002000 Lysosome-assoc_membr_glycop
PANTHERiPTHR11506 PTHR11506, 1 hit
PfamiView protein in Pfam
PF01299 Lamp, 1 hit
PRINTSiPR00336 LYSASSOCTDMP
PROSITEiView protein in PROSITE
PS00311 LAMP_2, 1 hit
PS51407 LAMP_3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform Long (identifier: P31996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRLPVCLILL GPLIAQGTEE DCPHKKAVTL LPSFTMTPTA TESTASPTTS
60 70 80 90 100
HRPTTTSHGN VTVHTSSGPT TVTHNPATTT SHGNATISHA TVSPTTNGTA
110 120 130 140 150
TSPRSSTVGP HPGPPPPSPS PRSKGALGNY TWANGSQPCV QLQAQIQIRI
160 170 180 190 200
LYPIQGGRKA WGISVLNPNK TKVQGGCDGT HPHLSLSFPY GQLTFGFKQD
210 220 230 240 250
LHQSPSTVYL DYMAVEYNVS FPQAAQWTFM AQNSSLRELQ APLGQSFCCG
260 270 280 290 300
NASIVLSPAV HLDLLSLRLQ AAQLPDKGHF GPCFSCNRDQ SLLLPLIIGL
310 320
VLLGLLTLVL IAFCITRRRQ STYQPL
Length:326
Mass (Da):34,818
Last modified:July 1, 1993 - v1
Checksum:iAB7203A9A7EA47BA
GO
Isoform Short (identifier: P31996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-326: Missing.

Show »
Length:318
Mass (Da):33,844
Checksum:iCB1AF96E96BD1DE9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J1C8A0A0R4J1C8_MOUSE
Macrosialin
Cd68
335Annotation score:

Sequence cautioni

The sequence BAA23738 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117P → T in AAC15685 (PubMed:9806844).Curated1
Sequence conflicti129N → K in BAB21975 (PubMed:16141072).Curated1
Sequence conflicti137Q → H in AAC15685 (PubMed:9806844).Curated1
Sequence conflicti205P → T in BAB21975 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003043319 – 326Missing in isoform Short. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA Translation: CAA48334.1
AF022651 Genomic DNA Translation: AAC40151.1
AF045554 Genomic DNA Translation: AAC15685.1
AF039399 Genomic DNA Translation: AAC40056.1
AB009287 Genomic DNA Translation: BAA23738.1 Sequence problems.
AK002264 mRNA Translation: BAB21975.1
AK170443 mRNA Translation: BAE41801.1
AL603707 Genomic DNA Translation: CAI51942.1
BC021637 mRNA Translation: AAH21637.1
DQ167574 mRNA Translation: ABA02181.1
CCDSiCCDS70216.1 [P31996-1]
PIRiA46676
RefSeqiNP_001277987.1, NM_001291058.1 [P31996-1]
UniGeneiMm.15819

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774 [P31996-1]
GeneIDi12514
KEGGimmu:12514
UCSCiuc007jqy.2 mouse [P31996-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA Translation: CAA48334.1
AF022651 Genomic DNA Translation: AAC40151.1
AF045554 Genomic DNA Translation: AAC15685.1
AF039399 Genomic DNA Translation: AAC40056.1
AB009287 Genomic DNA Translation: BAA23738.1 Sequence problems.
AK002264 mRNA Translation: BAB21975.1
AK170443 mRNA Translation: BAE41801.1
AL603707 Genomic DNA Translation: CAI51942.1
BC021637 mRNA Translation: AAH21637.1
DQ167574 mRNA Translation: ABA02181.1
CCDSiCCDS70216.1 [P31996-1]
PIRiA46676
RefSeqiNP_001277987.1, NM_001291058.1 [P31996-1]
UniGeneiMm.15819

3D structure databases

ProteinModelPortaliP31996
SMRiP31996
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31996, 1 interactor
MINTiP31996
STRINGi10090.ENSMUSP00000104294

PTM databases

iPTMnetiP31996
PhosphoSitePlusiP31996
SwissPalmiP31996

Proteomic databases

MaxQBiP31996
PaxDbiP31996
PeptideAtlasiP31996
PRIDEiP31996

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774 [P31996-1]
GeneIDi12514
KEGGimmu:12514
UCSCiuc007jqy.2 mouse [P31996-1]

Organism-specific databases

CTDi968
MGIiMGI:88342 Cd68

Phylogenomic databases

eggNOGiKOG4818 Eukaryota
ENOG410XQ96 LUCA
GeneTreeiENSGT00530000063068
HOGENOMiHOG000070034
HOVERGENiHBG005287
InParanoidiP31996
KOiK06501
PhylomeDBiP31996
TreeFamiTF316339

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:P31996
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018774 Expressed in 185 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_CD68
ExpressionAtlasiP31996 baseline and differential
GenevisibleiP31996 MM

Family and domain databases

InterProiView protein in InterPro
IPR018134 LAMP_CS
IPR002000 Lysosome-assoc_membr_glycop
PANTHERiPTHR11506 PTHR11506, 1 hit
PfamiView protein in Pfam
PF01299 Lamp, 1 hit
PRINTSiPR00336 LYSASSOCTDMP
PROSITEiView protein in PROSITE
PS00311 LAMP_2, 1 hit
PS51407 LAMP_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD68_MOUSE
AccessioniPrimary (citable) accession number: P31996
Secondary accession number(s): O54688
, O70321, Q3S4A9, Q5F2A8, Q9DD15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 12, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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