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Protein

Tautomerase PptA

Gene

pptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can use enol isomers of phenylpyruvate, 2-hydroxy-2,4-pentadienoate and (p-hydroxyphenyl)pyruvate as substrates.1 Publication

Enzyme regulationi

Inhibited by (E)-2-fluoro-p-hydroxycinnamate.1 Publication

Kineticsi

  1. KM=205 µM for enol isomers of phenylpyruvate1 Publication
  2. KM=220 µM for 2-hydroxy-2,4-pentadienoate1 Publication
  3. KM=180 µM for (p-hydroxyphenyl)pyruvate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei2Proton acceptor; via imino nitrogen1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11761-MONOMER
    SABIO-RKiP31992

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tautomerase PptA (EC:5.3.2.-)
    Gene namesi
    Name:pptA
    Synonyms:ydcE
    Ordered Locus Names:b1461, JW1456
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11761 pptA

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB02384 (E)-2-Fluoro-P-Hydroxycinnamate
    DB03793 Benzoic Acid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved
    ChainiPRO_00002095262 – 77Tautomerase PptAAdd BLAST76

    Proteomic databases

    PaxDbiP31992
    PRIDEiP31992

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262891, 27 interactors
    IntActiP31992, 14 interactors
    STRINGi316385.ECDH10B_1592

    Structurei

    Secondary structure

    177
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 9Combined sources7
    Helixi15 – 33Combined sources19
    Helixi37 – 39Combined sources3
    Beta strandi41 – 46Combined sources6
    Helixi49 – 51Combined sources3
    Helixi52 – 58Combined sources7
    Turni59 – 66Combined sources8

    3D structure databases

    ProteinModelPortaliP31992
    SMRiP31992
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31992

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105KZK Bacteria
    COG1942 LUCA
    HOGENOMiHOG000077848
    KOiK01821
    OMAiIKCFPRD

    Family and domain databases

    Gene3Di3.30.429.10, 1 hit
    HAMAPiMF_00718 Tautomerase_PptA, 1 hit
    InterProiView protein in InterPro
    IPR004370 4-oxalocrotonate_tautomerase
    IPR014347 Tautomerase/MIF_sf
    IPR017284 Tautomerase_PptA
    PfamiView protein in Pfam
    PF01361 Tautomerase, 1 hit
    PIRSFiPIRSF037799 Tautomer_YdcE_prd, 1 hit
    SUPFAMiSSF55331 SSF55331, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31992-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE
    60 70
    SWQAIWDAEI APQMEALIKK PGYSMNA
    Length:77
    Mass (Da):8,673
    Last modified:January 23, 2007 - v3
    Checksum:iD32FF4023FE556CA
    GO

    Mass spectrometryi

    Molecular mass is 8540 Da from positions 2 - 77. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X60998 Genomic DNA Translation: CAA43312.1
    U00096 Genomic DNA Translation: AAC74543.1
    AP009048 Genomic DNA Translation: BAA15092.1
    PIRiH64898
    RefSeqiNP_415978.1, NC_000913.3
    WP_001120143.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74543; AAC74543; b1461
    BAA15092; BAA15092; BAA15092
    GeneIDi945731
    KEGGiecj:JW1456
    eco:b1461
    PATRICifig|1411691.4.peg.807

    Similar proteinsi

    Entry informationi

    Entry nameiPPTA_ECOLI
    AccessioniPrimary (citable) accession number: P31992
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: June 20, 2018
    This is version 134 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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