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UniProtKB - P31992 (PPTA_ECOLI)
Protein
Tautomerase PptA
Gene
pptA
Organism
Escherichia coli (strain K12)
Status
Functioni
Can use enol isomers of phenylpyruvate, 2-hydroxy-2,4-pentadienoate and (p-hydroxyphenyl)pyruvate as substrates.
1 PublicationActivity regulationi
Inhibited by (E)-2-fluoro-p-hydroxycinnamate.1 Publication
Kineticsi
- KM=205 µM for enol isomers of phenylpyruvate1 Publication
- KM=220 µM for 2-hydroxy-2,4-pentadienoate1 Publication
- KM=180 µM for (p-hydroxyphenyl)pyruvate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | Proton acceptor; via imino nitrogen | 1 |
GO - Molecular functioni
GO - Biological processi
- cellular aromatic compound metabolic process Source: InterPro
Keywordsi
Molecular function | Isomerase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11761-MONOMER |
SABIO-RKi | P31992 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pptA Synonyms:ydcE Ordered Locus Names:b1461, JW1456 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000209526 | 2 – 77 | Tautomerase PptAAdd BLAST | 76 |
Proteomic databases
jPOSTi | P31992 |
PaxDbi | P31992 |
PRIDEi | P31992 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262891, 27 interactors |
IntActi | P31992, 14 interactors |
STRINGi | 511145.b1461 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P31992 |
SMRi | P31992 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31992 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1942, Bacteria |
HOGENOMi | CLU_183611_0_1_6 |
OMAi | IKCFPRD |
Family and domain databases
Gene3Di | 3.30.429.10, 1 hit |
HAMAPi | MF_00718, Tautomerase_PptA, 1 hit |
InterProi | View protein in InterPro IPR004370, 4-oxalocrotonate_tautomerase IPR014347, Tautomerase/MIF_sf IPR017284, Tautomerase_PptA |
Pfami | View protein in Pfam PF01361, Tautomerase, 1 hit |
PIRSFi | PIRSF037799, Tautomer_YdcE_prd, 1 hit |
SUPFAMi | SSF55331, SSF55331, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31992-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE
60 70
SWQAIWDAEI APQMEALIKK PGYSMNA
Mass spectrometryi
Molecular mass is 8540 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60998 Genomic DNA Translation: CAA43312.1 U00096 Genomic DNA Translation: AAC74543.1 AP009048 Genomic DNA Translation: BAA15092.1 |
PIRi | H64898 |
RefSeqi | NP_415978.1, NC_000913.3 WP_001120143.1, NZ_SSZK01000038.1 |
Genome annotation databases
EnsemblBacteriai | AAC74543; AAC74543; b1461 BAA15092; BAA15092; BAA15092 |
GeneIDi | 945731 |
KEGGi | ecj:JW1456 eco:b1461 |
PATRICi | fig|1411691.4.peg.807 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60998 Genomic DNA Translation: CAA43312.1 U00096 Genomic DNA Translation: AAC74543.1 AP009048 Genomic DNA Translation: BAA15092.1 |
PIRi | H64898 |
RefSeqi | NP_415978.1, NC_000913.3 WP_001120143.1, NZ_SSZK01000038.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GYJ | X-ray | 2.10 | A/B | 2-77 | [»] | |
1GYX | X-ray | 1.35 | A/B | 2-77 | [»] | |
1GYY | X-ray | 1.35 | A/B | 2-77 | [»] | |
AlphaFoldDBi | P31992 | |||||
SMRi | P31992 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262891, 27 interactors |
IntActi | P31992, 14 interactors |
STRINGi | 511145.b1461 |
Chemistry databases
DrugBanki | DB02384, (E)-2-Fluoro-P-Hydroxycinnamate |
Proteomic databases
jPOSTi | P31992 |
PaxDbi | P31992 |
PRIDEi | P31992 |
Genome annotation databases
EnsemblBacteriai | AAC74543; AAC74543; b1461 BAA15092; BAA15092; BAA15092 |
GeneIDi | 945731 |
KEGGi | ecj:JW1456 eco:b1461 |
PATRICi | fig|1411691.4.peg.807 |
Organism-specific databases
EchoBASEi | EB1711 |
Phylogenomic databases
eggNOGi | COG1942, Bacteria |
HOGENOMi | CLU_183611_0_1_6 |
OMAi | IKCFPRD |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11761-MONOMER |
SABIO-RKi | P31992 |
Miscellaneous databases
EvolutionaryTracei | P31992 |
PROi | PR:P31992 |
Family and domain databases
Gene3Di | 3.30.429.10, 1 hit |
HAMAPi | MF_00718, Tautomerase_PptA, 1 hit |
InterProi | View protein in InterPro IPR004370, 4-oxalocrotonate_tautomerase IPR014347, Tautomerase/MIF_sf IPR017284, Tautomerase_PptA |
Pfami | View protein in Pfam PF01361, Tautomerase, 1 hit |
PIRSFi | PIRSF037799, Tautomer_YdcE_prd, 1 hit |
SUPFAMi | SSF55331, SSF55331, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPTA_ECOLI | |
Accessioni | P31992Primary (citable) accession number: P31992 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families