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UniProtKB - P31979 (NUOF_ECOLI)

Entry version 160 (10 Feb 2021)
Sequence version 3 (01 Nov 1997)
Previous versions | rss
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Protein

NADH-quinone oxidoreductase subunit F

Gene

nuoF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi351Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi354Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi357Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi398Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi61 – 70NADBy similarity10
Nucleotide bindingi174 – 221FMNBy similarityAdd BLAST48

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTranslocase
Ligand4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:NUOF-MONOMER
MetaCyc:NUOF-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit F (EC:7.1.1.-)
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
NUO6
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nuoF
Ordered Locus Names:b2284, JW2279
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001185681 – 445NADH-quinone oxidoreductase subunit FAdd BLAST445

Keywords - PTMi

Quinone

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P31979

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P31979

PRoteomics IDEntifications database

More...PRIDEi		P31979

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260513, 64 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-243, Respiratory chain complex I

Database of interacting proteins

More...DIPi		DIP-10382N

Protein interaction database and analysis system

More...IntActi		P31979, 7 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2284

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P31979

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1894, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_014881_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P31979

Database for complete collections of gene phylogenies

More...PhylomeDBi		P31979

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1440.230, 1 hit
3.40.50.11540, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001949, NADH-UbQ_OxRdtase_51kDa_CS
IPR011537, NADH-UbQ_OxRdtase_suF
IPR011538, Nuo51_FMN-bd
IPR037225, Nuo51_FMN-bd_sf
IPR019575, Nuop51_4Fe4S-bd
IPR037207, Nuop51_4Fe4S-bd_sf
IPR019554, Soluble_ligand-bd

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01512, Complex1_51K, 1 hit
PF10589, NADH_4Fe-4S, 1 hit
PF10531, SLBB, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00928, NADH_4Fe-4S, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF140490, SSF140490, 1 hit
SSF142019, SSF142019, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01959, nuoF_fam, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00644, COMPLEX1_51K_1, 1 hit
PS00645, COMPLEX1_51K_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P31979-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKNIIRTPET HPLTWRLRDD KQPVWLDEYR SKNGYEGARK ALTGLSPDEI 
        60         70         80         90        100
VNQVKDAGLK GRGGAGFSTG LKWSLMPKDE SMNIRYLLCN ADEMEPGTYK 
       110        120        130        140        150
DRLLMEQLPH LLVEGMLISA FALKAYRGYI FLRGEYIEAA VNLRRAIAEA 
       160        170        180        190        200
TEAGLLGKNI MGTGFDFELF VHTGAGRYIC GEETALINSL EGRRANPRSK 
       210        220        230        240        250
PPFPATSGAW GKPTCVNNVE TLCNVPAILA NGVEWYQNIS KSKDAGTKLM 
       260        270        280        290        300
GFSGRVKNPG LWELPFGTTA REILEDYAGG MRDGLKFKAW QPGGAGTDFL 
       310        320        330        340        350
TEAHLDLPME FESIGKAGSR LGTALAMAVD HEINMVSLVR NLEEFFARES 
       360        370        380        390        400
CGWCTPCRDG LPWSVKILRA LERGEGQPGD IETLEQLCRF LGPGKTFCAH 
       410        420        430        440 
APGAVEPLQS AIKYFREEFE AGIKQPFSNT HLINGIQPNL LKERW
Length:445
Mass (Da):49,292
Last modified:November 1, 1997 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7F39A7DBE50C2075
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti3 – 11NIIRTPETH → KHYPYSRND in AAA53584 (PubMed:8366049).Curated9
Sequence conflicti26L → R in CAA48365 (PubMed:7690854).Curated1
Sequence conflicti38A → R in AAA53584 (PubMed:8366049).Curated1
Sequence conflicti41A → R in CAA48365 (PubMed:7690854).Curated1
Sequence conflicti51V → I in AAA53584 (PubMed:8366049).Curated1
Sequence conflicti370A → R (PubMed:7690854).Curated1
Sequence conflicti370A → R (PubMed:8157582).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48365.1
L25055 Unassigned DNA Translation: AAA03537.1
U00096 Genomic DNA Translation: AAC75344.1
AP009048 Genomic DNA Translation: BAA16113.1
L19569 Genomic DNA Translation: AAA53584.1

Protein sequence database of the Protein Information Resource

More...PIRi		B65000

NCBI Reference Sequences

More...RefSeqi		NP_416787.1, NC_000913.3
WP_000789500.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75344; AAC75344; b2284
BAA16113; BAA16113; BAA16113

Database of genes from NCBI RefSeq genomes

More...GeneIDi		946753

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2279
eco:b2284

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.4452

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48365.1
L25055 Unassigned DNA Translation: AAA03537.1
U00096 Genomic DNA Translation: AAC75344.1
AP009048 Genomic DNA Translation: BAA16113.1
L19569 Genomic DNA Translation: AAA53584.1
PIRiB65000
RefSeqiNP_416787.1, NC_000913.3
WP_000789500.1, NZ_LN832404.1

3D structure databases

SMRiP31979
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4260513, 64 interactors
ComplexPortaliCPX-243, Respiratory chain complex I
DIPiDIP-10382N
IntActiP31979, 7 interactors
STRINGi511145.b2284

Protein family/group databases

TCDBi3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Proteomic databases

jPOSTiP31979
PaxDbiP31979
PRIDEiP31979

Genome annotation databases

EnsemblBacteriaiAAC75344; AAC75344; b2284
BAA16113; BAA16113; BAA16113
GeneIDi946753
KEGGiecj:JW2279
eco:b2284
PATRICifig|1411691.4.peg.4452

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB1723

Phylogenomic databases

eggNOGiCOG1894, Bacteria
HOGENOMiCLU_014881_0_1_6
InParanoidiP31979
PhylomeDBiP31979

Enzyme and pathway databases

BioCyciEcoCyc:NUOF-MONOMER
MetaCyc:NUOF-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P31979

Family and domain databases

Gene3Di1.20.1440.230, 1 hit
3.40.50.11540, 1 hit
InterProiView protein in InterPro
IPR001949, NADH-UbQ_OxRdtase_51kDa_CS
IPR011537, NADH-UbQ_OxRdtase_suF
IPR011538, Nuo51_FMN-bd
IPR037225, Nuo51_FMN-bd_sf
IPR019575, Nuop51_4Fe4S-bd
IPR037207, Nuop51_4Fe4S-bd_sf
IPR019554, Soluble_ligand-bd
PfamiView protein in Pfam
PF01512, Complex1_51K, 1 hit
PF10589, NADH_4Fe-4S, 1 hit
PF10531, SLBB, 1 hit
SMARTiView protein in SMART
SM00928, NADH_4Fe-4S, 1 hit
SUPFAMiSSF140490, SSF140490, 1 hit
SSF142019, SSF142019, 1 hit
TIGRFAMsiTIGR01959, nuoF_fam, 1 hit
PROSITEiView protein in PROSITE
PS00644, COMPLEX1_51K_1, 1 hit
PS00645, COMPLEX1_51K_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNUOF_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P31979
Secondary accession number(s): P78239
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: February 10, 2021
This is version 160 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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