UniProtKB - P31979 (NUOF_ECOLI)
Protein
NADH-quinone oxidoreductase subunit F
Gene
nuoF
Organism
Escherichia coli (strain K12)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- FMNCuratedNote: Binds 1 FMN.Curated
- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 351 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 354 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 357 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 398 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 61 – 70 | NADBy similarity | 10 | |
Nucleotide bindingi | 174 – 221 | FMNBy similarityAdd BLAST | 48 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- FMN binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- NAD binding Source: EcoCyc
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- aerobic respiration Source: EcoCyc
- cellular respiration Source: GO_Central
Keywordsi
Molecular function | Translocase |
Ligand | 4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOF-MONOMER MetaCyc:NUOF-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit F (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit F NDH-1 subunit F NUO6 |
Gene namesi | Name:nuoF Ordered Locus Names:b2284, JW2279 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- NADH dehydrogenase complex Source: EcoliWiki
- plasma membrane Source: EcoliWiki
- plasma membrane respiratory chain complex I Source: EcoCyc
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118568 | 1 – 445 | NADH-quinone oxidoreductase subunit FAdd BLAST | 445 |
Keywords - PTMi
QuinoneProteomic databases
jPOSTi | P31979 |
PaxDbi | P31979 |
PRIDEi | P31979 |
Interactioni
Subunit structurei
Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Protein-protein interaction databases
BioGRIDi | 4260513, 64 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-10382N |
IntActi | P31979, 7 interactors |
STRINGi | 511145.b2284 |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I 51 kDa subunit family.Curated
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_0_1_6 |
InParanoidi | P31979 |
PhylomeDBi | P31979 |
Family and domain databases
Gene3Di | 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011537, NADH-UbQ_OxRdtase_suF IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR019554, Soluble_ligand-bd |
PANTHERi | PTHR11780, PTHR11780, 1 hit |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit PF10531, SLBB, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit |
TIGRFAMsi | TIGR01959, nuoF_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P31979-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNIIRTPET HPLTWRLRDD KQPVWLDEYR SKNGYEGARK ALTGLSPDEI
60 70 80 90 100
VNQVKDAGLK GRGGAGFSTG LKWSLMPKDE SMNIRYLLCN ADEMEPGTYK
110 120 130 140 150
DRLLMEQLPH LLVEGMLISA FALKAYRGYI FLRGEYIEAA VNLRRAIAEA
160 170 180 190 200
TEAGLLGKNI MGTGFDFELF VHTGAGRYIC GEETALINSL EGRRANPRSK
210 220 230 240 250
PPFPATSGAW GKPTCVNNVE TLCNVPAILA NGVEWYQNIS KSKDAGTKLM
260 270 280 290 300
GFSGRVKNPG LWELPFGTTA REILEDYAGG MRDGLKFKAW QPGGAGTDFL
310 320 330 340 350
TEAHLDLPME FESIGKAGSR LGTALAMAVD HEINMVSLVR NLEEFFARES
360 370 380 390 400
CGWCTPCRDG LPWSVKILRA LERGEGQPGD IETLEQLCRF LGPGKTFCAH
410 420 430 440
APGAVEPLQS AIKYFREEFE AGIKQPFSNT HLINGIQPNL LKERW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 – 11 | NIIRTPETH → KHYPYSRND in AAA53584 (PubMed:8366049).Curated | 9 | |
Sequence conflicti | 26 | L → R in CAA48365 (PubMed:7690854).Curated | 1 | |
Sequence conflicti | 38 | A → R in AAA53584 (PubMed:8366049).Curated | 1 | |
Sequence conflicti | 41 | A → R in CAA48365 (PubMed:7690854).Curated | 1 | |
Sequence conflicti | 51 | V → I in AAA53584 (PubMed:8366049).Curated | 1 | |
Sequence conflicti | 370 | A → R (PubMed:7690854).Curated | 1 | |
Sequence conflicti | 370 | A → R (PubMed:8157582).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48365.1 L25055 Unassigned DNA Translation: AAA03537.1 U00096 Genomic DNA Translation: AAC75344.1 AP009048 Genomic DNA Translation: BAA16113.1 L19569 Genomic DNA Translation: AAA53584.1 |
PIRi | B65000 |
RefSeqi | NP_416787.1, NC_000913.3 WP_000789500.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75344; AAC75344; b2284 BAA16113; BAA16113; BAA16113 |
GeneIDi | 48724650 946753 |
KEGGi | ecj:JW2279 eco:b2284 |
PATRICi | fig|1411691.4.peg.4452 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48365.1 L25055 Unassigned DNA Translation: AAA03537.1 U00096 Genomic DNA Translation: AAC75344.1 AP009048 Genomic DNA Translation: BAA16113.1 L19569 Genomic DNA Translation: AAA53584.1 |
PIRi | B65000 |
RefSeqi | NP_416787.1, NC_000913.3 WP_000789500.1, NZ_LN832404.1 |
3D structure databases
SMRi | P31979 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260513, 64 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-10382N |
IntActi | P31979, 7 interactors |
STRINGi | 511145.b2284 |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Proteomic databases
jPOSTi | P31979 |
PaxDbi | P31979 |
PRIDEi | P31979 |
Genome annotation databases
EnsemblBacteriai | AAC75344; AAC75344; b2284 BAA16113; BAA16113; BAA16113 |
GeneIDi | 48724650 946753 |
KEGGi | ecj:JW2279 eco:b2284 |
PATRICi | fig|1411691.4.peg.4452 |
Organism-specific databases
EchoBASEi | EB1723 |
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_0_1_6 |
InParanoidi | P31979 |
PhylomeDBi | P31979 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOF-MONOMER MetaCyc:NUOF-MONOMER |
Miscellaneous databases
PROi | PR:P31979 |
Family and domain databases
Gene3Di | 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011537, NADH-UbQ_OxRdtase_suF IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR019554, Soluble_ligand-bd |
PANTHERi | PTHR11780, PTHR11780, 1 hit |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit PF10531, SLBB, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit |
TIGRFAMsi | TIGR01959, nuoF_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOF_ECOLI | |
Accessioni | P31979Primary (citable) accession number: P31979 Secondary accession number(s): P78239 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families