UniProtKB - P31977 (EZRI_RAT)
Ezrin
Ezr
Functioni
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis (By similarity).
By similarityActivity regulationi
GO - Molecular functioni
- actin binding Source: RGD
- actin filament binding Source: UniProtKB
- ATPase binding Source: RGD
- cell adhesion molecule binding Source: UniProtKB
- disordered domain specific binding Source: RGD
- identical protein binding Source: RGD
- microtubule binding Source: RGD
- protein-containing complex binding Source: RGD
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase A binding Source: RGD
- protein kinase A catalytic subunit binding Source: RGD
- protein kinase A regulatory subunit binding Source: RGD
- S100 protein binding Source: RGD
- structural molecule activity Source: RGD
GO - Biological processi
- actin cytoskeleton reorganization Source: RGD
- actin filament bundle assembly Source: UniProtKB
- astral microtubule organization Source: RGD
- cellular response to cAMP Source: RGD
- cortical microtubule organization Source: RGD
- epithelial cell differentiation Source: RGD
- establishment of centrosome localization Source: RGD
- establishment of endothelial barrier Source: RGD
- establishment or maintenance of apical/basal cell polarity Source: RGD
- filopodium assembly Source: RGD
- intestinal D-glucose absorption Source: RGD
- leukocyte cell-cell adhesion Source: RGD
- membrane to membrane docking Source: RGD
- microvillus assembly Source: RGD
- negative regulation of ERK1 and ERK2 cascade Source: RGD
- negative regulation of interleukin-2 production Source: RGD
- negative regulation of p38MAPK cascade Source: RGD
- negative regulation of T cell receptor signaling pathway Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- phosphatidylinositol-mediated signaling Source: RGD
- positive regulation of cellular protein catabolic process Source: RGD
- positive regulation of early endosome to late endosome transport Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of multicellular organism growth Source: RGD
- positive regulation of protein localization to early endosome Source: RGD
- positive regulation of protein localization to plasma membrane Source: RGD
- positive regulation of protein secretion Source: RGD
- protein-containing complex localization Source: RGD
- protein kinase A signaling Source: RGD
- protein localization to cell cortex Source: RGD
- protein localization to plasma membrane Source: RGD
- receptor internalization Source: RGD
- regulation of cell shape Source: RGD
- regulation of microvillus length Source: RGD
- regulation of organelle assembly Source: RGD
- sphingosine-1-phosphate receptor signaling pathway Source: RGD
- terminal web assembly Source: RGD
Keywordsi
Biological process | Cell shape |
Enzyme and pathway databases
Reactomei | R-RNO-373752, Netrin-1 signaling R-RNO-437239, Recycling pathway of L1 |
Names & Taxonomyi
Protein namesi | Recommended name: EzrinAlternative name(s): Cytovillin Villin-2 p81 |
Gene namesi | Name:Ezr Synonyms:Vil2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621161, Ezr |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- microvillus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- ruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoskeleton
- cytoskeleton By similarity
Other locations
- Cell projection By similarity
- cell cortex By similarity
- microvillus By similarity
Note: Localization to the apical membrane of parietal cells depends on the interaction with PALS1. Microvillar peripheral membrane protein (cytoplasmic side) (By similarity). Localizes to cell extensions and peripheral processes of astrocytes. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells.By similarity2 Publications
Cytoskeleton
- actin cytoskeleton Source: UniProtKB
- actin filament Source: UniProtKB
- ciliary basal body Source: RGD
Cytosol
- cytosol Source: RGD
Endosome
- endosome Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- basolateral plasma membrane Source: UniProtKB
- cytoplasmic side of apical plasma membrane Source: RGD
- immunological synapse Source: RGD
- microvillus membrane Source: UniProtKB
- plasma membrane Source: RGD
- plasma membrane raft Source: RGD
- ruffle membrane Source: UniProtKB-SubCell
- T-tubule Source: RGD
Other locations
- adherens junction Source: GO_Central
- apical part of cell Source: RGD
- astrocyte projection Source: RGD
- brush border Source: RGD
- cell body Source: RGD
- cell cortex Source: UniProtKB-SubCell
- cell periphery Source: RGD
- cell projection Source: RGD
- cell tip Source: RGD
- cytoplasm Source: RGD
- extrinsic component of membrane Source: UniProtKB
- filopodium Source: RGD
- membrane raft Source: RGD
- microspike Source: RGD
- microvillus Source: RGD
- perinuclear region of cytoplasm Source: RGD
- protein-containing complex Source: RGD
- ruffle Source: RGD
- Schwann cell microvillus Source: RGD
- uropod Source: RGD
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219411 | 1 – 586 | EzrinAdd BLAST | 586 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 146 | Phosphotyrosine; by PDGFRBy similarity | 1 | |
Modified residuei | 366 | PhosphoserineBy similarity | 1 | |
Modified residuei | 478 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 535 | PhosphoserineCombined sources | 1 | |
Modified residuei | 567 | Phosphothreonine; by ROCK2 and PKC/PRKCIBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, S-nitrosylationProteomic databases
jPOSTi | P31977 |
PaxDbi | P31977 |
PRIDEi | P31977 |
2D gel databases
World-2DPAGEi | 0004:P31977 |
PTM databases
iPTMneti | P31977 |
PhosphoSitePlusi | P31977 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSRNOG00000018524, Expressed in stomach and 20 other tissues |
ExpressionAtlasi | P31977, baseline and differential |
Genevisiblei | P31977, RN |
Interactioni
Subunit structurei
Interacts with PODXL and SLC9A3R2.
Found in a complex with EZR, PODXL and SLC9A3R2 (PubMed:11457882).
Interacts with PALS1.
Interacts with MCC, PLEKHG6, SCYL3/PACE1, SLC9A3R1 and TMEM8B.
Interacts (when phosphorylated) with FES/FPS.
Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin. Detected in a complex composed of at least EZR, AHNAK, PPL and PRX.
Interacts with PDPN (via cytoplasmic domain); activates RHOA and promotes epithelial-mesenchymal transition.
Interacts with SPN/CD43 cytoplasmic tail, CD44 and ICAM2 (By similarity).
By similarity1 PublicationBinary interactionsi
P31977
With | #Exp. | IntAct |
---|---|---|
Dcc [Q63155] | 2 | EBI-917242,EBI-1798965 |
GO - Molecular functioni
- actin binding Source: RGD
- actin filament binding Source: UniProtKB
- ATPase binding Source: RGD
- cell adhesion molecule binding Source: UniProtKB
- disordered domain specific binding Source: RGD
- identical protein binding Source: RGD
- microtubule binding Source: RGD
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase A binding Source: RGD
- protein kinase A catalytic subunit binding Source: RGD
- protein kinase A regulatory subunit binding Source: RGD
- S100 protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248535, 2 interactors |
CORUMi | P31977 |
IntActi | P31977, 3 interactors |
STRINGi | 10116.ENSRNOP00000046593 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 295 | FERMPROSITE-ProRule annotationAdd BLAST | 294 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 244 – 586 | Interaction with SCYL3By similarityAdd BLAST | 343 | |
Regioni | 306 – 338 | DisorderedSequence analysisAdd BLAST | 33 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 302 – 462 | Sequence analysisAdd BLAST | 161 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 115 – 120 | [IL]-x-C-x-x-[DE] motifBy similarity | 6 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3529, Eukaryota |
GeneTreei | ENSGT00960000186596 |
HOGENOMi | CLU_003623_6_2_1 |
InParanoidi | P31977 |
OMAi | HSGMLRE |
OrthoDBi | 627741at2759 |
PhylomeDBi | P31977 |
TreeFami | TF313935 |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit cd13194, FERM_C_ERM, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 2.30.29.30, 1 hit 6.10.360.10, 1 hit |
InterProi | View protein in InterPro IPR019749, Band_41_domain IPR011174, ERM IPR041789, ERM_FERM_C IPR000798, Ez/rad/moesin-like IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR008954, Moesin_tail_sf IPR011993, PH-like_dom_sf IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23281, PTHR23281, 1 hit |
Pfami | View protein in Pfam PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit |
PIRSFi | PIRSF002305, ERM, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF48678, SSF48678, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWYFGLQYV
60 70 80 90 100
DNKGFPTWLK LDKKVSAQEV RKENPVQFKF RAKFYPEDVA DELIQDITQK
110 120 130 140 150
LFFLQVKEGI LSDEIYCPPE TAVLLGSYAV QAKFGDYNKE MHKSGYLSSE
160 170 180 190 200
RLIPQRVMDQ HKLSRDQWED RIQVWHAEHR GMLKDSAMLE YLKIAQDLEM
210 220 230 240 250
YGINYFEIKN KKGTDLWLGV DALGLNIYEK DDKLTPKIGF PWSEIRNISF
260 270 280 290 300
NDKKFVIKPI DKKAPDFVFY APRLRINKRI LQLCMGNHEL YMRRRKPDTI
310 320 330 340 350
EVQQMKAQAR EEKHQKQLER QQLETEKKRR ETVEREKEQM LREKEELMLR
360 370 380 390 400
LQDFEQKTKR AEKELSEQIE KALQLEEERR RAQEEAERLE ADRMAALRAK
410 420 430 440 450
EELERQAQDQ IKSQEQLAAE LAEYTAKIAL LEEARRRKED EVEEWQHRAK
460 470 480 490 500
EAQDDLVKTK EELHLVMTAP PPPPPPVYEP VNYHVQEGLQ DEGAEPMGYS
510 520 530 540 550
AELSSEGILD DRNEEKRITE AEKNERVQRQ LLTLSNELSQ ARDENKRTHN
560 570 580
DIIHNENMRQ GRDKYKTLRQ IRQGNTKQRI DEFEAM
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K890 | A0A0G2K890_RAT | Ezrin | Ezr | 590 | Annotation score: | ||
A0A8I5Y4R7 | A0A8I5Y4R7_RAT | Ezrin | Ezr | 592 | Annotation score: | ||
A0A8I6A8I6 | A0A8I6A8I6_RAT | Ezrin | Ezr | 575 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 455 | D → E in AAL47844 (Ref. 3) Curated | 1 | |
Sequence conflicti | 531 | L → P in CAA48004 (PubMed:7640303).Curated | 1 | |
Sequence conflicti | 532 | L → Q in AAR91694 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY428869 mRNA Translation: AAR91694.1 BC081958 mRNA Translation: AAH81958.1 AF450298 mRNA Translation: AAL47844.1 X67788 mRNA Translation: CAA48004.1 |
PIRi | S58759 |
RefSeqi | NP_062230.1, NM_019357.1 |
Genome annotation databases
Ensembli | ENSRNOT00000046746; ENSRNOP00000046593; ENSRNOG00000018524 |
GeneIDi | 54319 |
KEGGi | rno:54319 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY428869 mRNA Translation: AAR91694.1 BC081958 mRNA Translation: AAH81958.1 AF450298 mRNA Translation: AAL47844.1 X67788 mRNA Translation: CAA48004.1 |
PIRi | S58759 |
RefSeqi | NP_062230.1, NM_019357.1 |
3D structure databases
AlphaFoldDBi | P31977 |
BMRBi | P31977 |
SMRi | P31977 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248535, 2 interactors |
CORUMi | P31977 |
IntActi | P31977, 3 interactors |
STRINGi | 10116.ENSRNOP00000046593 |
PTM databases
iPTMneti | P31977 |
PhosphoSitePlusi | P31977 |
2D gel databases
World-2DPAGEi | 0004:P31977 |
Proteomic databases
jPOSTi | P31977 |
PaxDbi | P31977 |
PRIDEi | P31977 |
Genome annotation databases
Ensembli | ENSRNOT00000046746; ENSRNOP00000046593; ENSRNOG00000018524 |
GeneIDi | 54319 |
KEGGi | rno:54319 |
Organism-specific databases
CTDi | 7430 |
RGDi | 621161, Ezr |
Phylogenomic databases
eggNOGi | KOG3529, Eukaryota |
GeneTreei | ENSGT00960000186596 |
HOGENOMi | CLU_003623_6_2_1 |
InParanoidi | P31977 |
OMAi | HSGMLRE |
OrthoDBi | 627741at2759 |
PhylomeDBi | P31977 |
TreeFami | TF313935 |
Enzyme and pathway databases
Reactomei | R-RNO-373752, Netrin-1 signaling R-RNO-437239, Recycling pathway of L1 |
Miscellaneous databases
PROi | PR:P31977 |
Gene expression databases
Bgeei | ENSRNOG00000018524, Expressed in stomach and 20 other tissues |
ExpressionAtlasi | P31977, baseline and differential |
Genevisiblei | P31977, RN |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit cd13194, FERM_C_ERM, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 2.30.29.30, 1 hit 6.10.360.10, 1 hit |
InterProi | View protein in InterPro IPR019749, Band_41_domain IPR011174, ERM IPR041789, ERM_FERM_C IPR000798, Ez/rad/moesin-like IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR008954, Moesin_tail_sf IPR011993, PH-like_dom_sf IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23281, PTHR23281, 1 hit |
Pfami | View protein in Pfam PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit |
PIRSFi | PIRSF002305, ERM, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF48678, SSF48678, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EZRI_RAT | |
Accessioni | P31977Primary (citable) accession number: P31977 Secondary accession number(s): Q5WQV4, Q66H97, Q8VHK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 184 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |