UniProtKB - P31801 (CHAA_ECOLI)
Protein
Sodium-potassium/proton antiporter ChaA
Gene
chaA
Organism
Escherichia coli (strain K12)
Status
Functioni
Sodium exporter that functions mainly at alkaline pH. Can also function as a potassium/proton and calcium/proton antiporter at alkaline pH. Does not play a major role in calcium export.5 Publications
Activity regulationi
Pronounced pH dependence. Inhibited by magnesium.1 Publication
GO - Molecular functioni
- potassium:proton antiporter activity Source: EcoCyc
- sodium:proton antiporter activity Source: EcoCyc
GO - Biological processi
- cellular potassium ion homeostasis Source: GO_Central
- cellular sodium ion homeostasis Source: GO_Central
- sodium ion transport Source: EcoCyc
Keywordsi
Biological process | Antiport, Ion transport, Transport |
Ligand | Calcium, Potassium, Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:CHAA-MONOMER MetaCyc:CHAA-MONOMER |
Protein family/group databases
TCDBi | 2.A.19.1.1, the ca(2+):cation antiporter (caca) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium-potassium/proton antiporter ChaACuratedAlternative name(s): Na(+)/H(+) exchanger |
Gene namesi | Name:chaA Ordered Locus Names:b1216, JW1207 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 38 – 58 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 59 – 74 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 75 – 95 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 96 – 106 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 107 – 127 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 128 – 143 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 144 – 164 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 165 – 167 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 168 – 188 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 189 – 216 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 217 – 237 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 238 – 255 | PeriplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 256 – 276 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 277 – 290 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 291 – 311 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 312 – 318 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 319 – 339 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 340 – 345 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 346 – 366 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209506 | 1 – 366 | Sodium-potassium/proton antiporter ChaAAdd BLAST | 366 |
Proteomic databases
PaxDbi | P31801 |
PRIDEi | P31801 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260120, 11 interactors |
STRINGi | 511145.b1216 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 200 – 209 | Asp/Glu-rich (acidic) | 10 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0387, Bacteria |
HOGENOMi | CLU_050648_0_0_6 |
InParanoidi | P31801 |
PhylomeDBi | P31801 |
Family and domain databases
InterProi | View protein in InterPro IPR004713, CaH_exchang IPR004837, NaCa_Exmemb |
Pfami | View protein in Pfam PF01699, Na_Ca_ex, 2 hits |
TIGRFAMsi | TIGR00846, caca2, 1 hit |
i Sequence
Sequence statusi: Complete.
P31801-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNAQEAVKT RHKETSLIFP VLALVVLFLW GSSQTLPVVI AINLLALIGI
60 70 80 90 100
LSSAFSVVRH ADVLAHRLGE PYGSLILSLS VVILEVSLIS ALMATGDAAP
110 120 130 140 150
TLMRDTLYSI IMIVTGGLVG FSLLLGGRKF ATQYMNLFGI KQYLIALFPL
160 170 180 190 200
AIIVLVFPMA LPAANFSTGQ ALLVALISAA MYGVFLLIQT KTHQSLFVYE
210 220 230 240 250
HEDDSDDDDP HHGKPSAHSS LWHAIWLIIH LIAVIAVTKM NASSLETLLD
260 270 280 290 300
SMNAPVAFTG FLVALLILSP EGLGALKAVL NNQVQRAMNL FFGSVLATIS
310 320 330 340 350
LTVPVVTLIA FMTGNELQFA LGAPEMVVMV ASLVLCHISF STGRTNVLNG
360
AAHLALFAAY LMTIFA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28709 Genomic DNA Translation: AAA20200.1 U00096 Genomic DNA Translation: AAC74300.1 AP009048 Genomic DNA Translation: BAA36074.1 |
PIRi | A46716 |
RefSeqi | NP_415734.1, NC_000913.3 WP_000063607.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74300; AAC74300; b1216 BAA36074; BAA36074; BAA36074 |
GeneIDi | 945790 |
KEGGi | ecj:JW1207 eco:b1216 |
PATRICi | fig|1411691.4.peg.1066 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28709 Genomic DNA Translation: AAA20200.1 U00096 Genomic DNA Translation: AAC74300.1 AP009048 Genomic DNA Translation: BAA36074.1 |
PIRi | A46716 |
RefSeqi | NP_415734.1, NC_000913.3 WP_000063607.1, NZ_SSZK01000010.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260120, 11 interactors |
STRINGi | 511145.b1216 |
Protein family/group databases
TCDBi | 2.A.19.1.1, the ca(2+):cation antiporter (caca) family |
Proteomic databases
PaxDbi | P31801 |
PRIDEi | P31801 |
Genome annotation databases
EnsemblBacteriai | AAC74300; AAC74300; b1216 BAA36074; BAA36074; BAA36074 |
GeneIDi | 945790 |
KEGGi | ecj:JW1207 eco:b1216 |
PATRICi | fig|1411691.4.peg.1066 |
Organism-specific databases
EchoBASEi | EB1703 |
Phylogenomic databases
eggNOGi | COG0387, Bacteria |
HOGENOMi | CLU_050648_0_0_6 |
InParanoidi | P31801 |
PhylomeDBi | P31801 |
Enzyme and pathway databases
BioCyci | EcoCyc:CHAA-MONOMER MetaCyc:CHAA-MONOMER |
Miscellaneous databases
PROi | PR:P31801 |
Family and domain databases
InterProi | View protein in InterPro IPR004713, CaH_exchang IPR004837, NaCa_Exmemb |
Pfami | View protein in Pfam PF01699, Na_Ca_ex, 2 hits |
TIGRFAMsi | TIGR00846, caca2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHAA_ECOLI | |
Accessioni | P31801Primary (citable) accession number: P31801 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families