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Protein

Cyclomaltodextrin glucanotransferase

Gene

cgt

Organism
Bacillus sp. (strain 6.6.3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Calcium 1By similarity1
Metal bindingi63Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi66Calcium 1By similarity1
Metal bindingi67Calcium 1By similarity1
Metal bindingi85Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi87Calcium 1By similarity1
Metal bindingi173Calcium 2By similarity1
Binding sitei174SubstrateBy similarity1
Metal bindingi224Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi233Calcium 2By similarity1
Binding sitei261SubstrateBy similarity1
Active sitei263NucleophileBy similarity1
Metal bindingi267Calcium 2; via carbonyl oxygenBy similarity1
Active sitei291Proton donorBy similarity1
Binding sitei361SubstrateBy similarity1
Sitei362Transition state stabilizerBy similarity1
Binding sitei405SubstrateBy similarity1
Binding sitei409SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19 691

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgt
OrganismiBacillus sp. (strain 6.6.3)
Taxonomic identifieri29335 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000000144135 – 718Cyclomaltodextrin glucanotransferaseAdd BLAST684

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 84By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP31747
SMRiP31747
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini532 – 612IPT/TIGAdd BLAST81
Domaini613 – 718CBM20PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 172A1Add BLAST138
Regioni134 – 135Substrate bindingBy similarity2
Regioni173 – 236BAdd BLAST64
Regioni227 – 230Substrate bindingBy similarity4
Regioni237 – 440A2Add BLAST204
Regioni266 – 267Substrate bindingBy similarity2
Regioni441 – 528CAdd BLAST88
Regioni529 – 614DAdd BLAST86
Regioni615 – 718EAdd BLAST104

Domaini

May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR006311 TAT_signal
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31747-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFQMAKRAFL STTLTLGLLA GSALPFLPAS AAYADPDIAV TNKQSFSTDV
60 70 80 90 100
IYQVFTDRFL DGNPSNNPTG AAYDATCSNL KLYCGGDWQG LINKINDNYF
110 120 130 140 150
SDLGVTALWI SQPVENIFAT INYSGVTNTA YHGYWARDFK KTNPYFGTMA
160 170 180 190 200
DFQNLITTAH AKGIKIIIDF APNHTSPAME TDTSFAENGK LYDNGTLVGG
210 220 230 240 250
YTNDTNGYFH HNGGSDFSSL ENGIYKNLYD LADFNHNNAT IDKYFKDAIK
260 270 280 290 300
LWLDMGVDGI RVDAVKHIAL GWQKSWMSSI YVHKPVFTFG EWFLGSAASD
310 320 330 340 350
ADNTDFANKS GMSLLDFRFN SAVRNVFRDN TSNMYALDSM INSTATDYNQ
360 370 380 390 400
VNDQVTFIDN HDMDRFKTSA VNNRRLEQAL AFTLTSRGVP AIYYGTEQYL
410 420 430 440 450
TGNGDPDNRA KMPSFSKSTT AFNVISKLAP LRKSNPAIAY GSTQQRWINN
460 470 480 490 500
DVYVYERKFG KSVAVVAVNR NLSTPANITG LSTSLPTGSY TDVLGGVLNG
510 520 530 540 550
NNITSSNGSV NSFTLAAGAT AVWQYTAAET TPTIGHVGPV MGKPGNVVTI
560 570 580 590 600
DGRGFGSTKG TVYFGTTAVT GAAITSWEDT QIKVTIPSVA AGNYAVKVAA
610 620 630 640 650
NGVNSNAYNH FTILTGDQVT VRFVINNAST TLGQNIYLTG NVAELGNWST
660 670 680 690 700
GSTAIGPAFN QVIHQYPTWY YDVSVPAGKE LEFKFFKKNG STITWEGGSN
710
HKFTTPASGT ATVTVNWQ
Length:718
Mass (Da):78,015
Last modified:July 1, 1993 - v1
Checksum:i7644096D402707E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66106 Genomic DNA Translation: CAA46901.1
PIRiS21532 ALBSG6

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66106 Genomic DNA Translation: CAA46901.1
PIRiS21532 ALBSG6

3D structure databases

ProteinModelPortaliP31747
SMRiP31747
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.19 691

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR006311 TAT_signal
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
PS51318 TAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDGT_BACSS
AccessioniPrimary (citable) accession number: P31747
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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